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IVES 9 IVES Conference Series 9 GiESCO 9 GiESCO 2015 9 GiESCO 2015 – Session 4: Grape berry, microclimate 9 The regulation of the grapevine berry transcriptome by temperature is dependent on the circadian cycle

The regulation of the grapevine berry transcriptome by temperature is dependent on the circadian cycle

Abstract

High throughput transcriptomic studies (microarray, RNA-seq) are powerful tools to decipher changes in gene expression during fruit development and to target candidate genes involved in the plant response to environmental stresses. However, the rapid RNA turnover rate constitutes a major drawback in transcriptomic studies upon introducing sensitivity to environmental and trophic changes within one day. Thus, their precision and reproducibility highly depends on the sampling protocol. The present study aimed to test the hypothesis that fast transcriptomic changes induced by heat stress may vary during the day in microvine berries. Berry development was characterized with whole genome microarrays on microvine plants grown under fully controlled environment. Developmentally regulated transcripts could be clustered the same way independently at day and night with 20 % more transcripts identified during the night. Strikingly day-night regulation varies to a high extent between green and ripening berries. Interestingly, an important shift in the day-night regulation of secondary metabolism was observed from green to ripening berries. For the first time, the present study revealed important circadian mechanisms related to secondary metabolism changing throughout fleshy fruit development.

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Publication date: 07/09/2026

Issue: GiESCO 2015

Type: Extended abstract

Authors

Markus Rienth1,2,3,*, Nathalie Luchaire2,4, Anne Pellegrino4, Gilbert Lopez2, Marc Farnos5, Catherine Roux5, Laurent Torregrosa2, Charles Romieu5

1 Fondation Jean Poupelain – Javrezac, France

2 Montpellier SupAgro, UMR AGAP, 2 place Pierre Viala, 34060 Montpellier, France; UMT Génovigne, INRA de Pech-Rouge, 11430 Gruissan, France

3 Changins, haute école de viticulture et œnologie, route de Dullier 50, 1260 Nyon, Suisse

4 Montpellier SupAgro, UMR LEPSE – Laboratoire d’Ecophysiologie des Plantes sous Stress Environnementaux, 2 Place Viala, F-34060 Montpellier, France

5 INRA, UMR AGAP, 2 place Pierre Viala, 34060 Montpellier, France

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Keywords

berry transcriptome, circadian rhythm, temperature, microvine, transcriptome

Tags

GiESCO | GiESCO 2015 | IVES Conference Series

Citation

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