Macrowine 2021
IVES 9 IVES Conference Series 9 Micro-meteorological, compositional and transcriptional study of corvina grape color during ripening

Micro-meteorological, compositional and transcriptional study of corvina grape color during ripening

Abstract

Grape anthocyanin content and composition could affect the quality and the production strategies of red wines. Differences in the pigment composition modify the color properties in terms of hue, extractability and stability. Thus, for the production of a highly qualitative wine such as “Amarone”, variations in the pigment composition are not negligible. The aim of this work was the investigation of the anthocyanin profile changes during ripening in Corvina grapes, the main cultivar for the “Amarone” production. The experiment took place in 2015, in two vineyards located in Valpollicella (Italy). Micro-meteorological survey, carpological characterization, chemical analysis and transcriptional studies were carried out to clarify the anthocyanin biosynthesis regulation. Air and grape temperatures and global solar radiation inside the canopy where measured during the season. Grape samples were collected at seven stages of berry development from pre-véraison until harvest. Berry growth was followed by both weight and volume increases. At veraison, anthocyanin quantification, by spectroscopy, and profile composition analysis, by HPLC, started (5 developmental stages were analyzed). The expression level of structural and regulatory genes of anthocyanin pathway was studied via real time polymerase chain reaction during all the seven development stages considered. Even if the vineyards are close each other the different training systems (“Guyot” and “Pergola”) created different microclimates for grape ripening. As an example, in “Pergola” the grape temperatures rarely exceeded 40°C. On the opposite, in “Guyot” the temperatures rise over 59°C in hottest days, and often overpassed 50°C. Despite these differences, the anthocyanin profile similarly changed during ripening among the two vineyards. At color appearance (average anthocyanin content: 80 mg kg-1 of grapes), the di-substituted anthocyanins prevailed. During ripening, it was possible to observe a progressive increase of tri-substituted, methoxylated, and acylated pigments. The transcriptional levels of “Guyot” and “Pergola” confirmed to be similar. A clear correlation among expression of anthocyanin biosynthetic gene UDPglucose:flavonoid 3-O-glucosyltransferase (UFGT), transcription factors, MYBA1 and MYBA2, and total anthocyanin content during berry development has been identified. Chalcone synthase, flavonoid 3ʹ-hydroxylase (F3ʹH) and flavonoid 3ʹ,5ʹ-hydroxylase (F3ʹ5ʹH) genes of the flavonoid pathway showed high correlation as well. The proportion changes between tri- and di-substituted anthocyaninswas associated with an increasing ratio of F3ʹ5ʹH/F3ʹH gene transcription during ripening. The AOMT genes were expressed with a maximum at the onset of ripening, coherently with the rapid increase of methoxylated anthocyanin proportion in this stage. Detailed knowledges of grape color variations during ripening could support the vineyard management techniques including harvesting time.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Laura Rustioni*, Federica Zoli, Gabriella De Lorenzis, Lucio Brancadoro, Simone Parisi

*Università di Milano

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Influence of methyl jasmonate foliar application to vineyard on grape volatile composition over three consecutive vintages

An alternative to improve grape quality is the application to the vineyard of elicitors. Although these compounds were first used to increase resistance of plants against pathogens, it has been found that they are also able to induce mechanisms involved in the synthesis of phenolic compounds and some amino acids. However, researches about the influence of elicitors on grape volatile composition are scarcely. Therefore, the aim of this work was to study the influence of methyl jasmonate (MeJ) foliar application on grape aroma composition over three consecutive vintages. MeJ was applied to Tempranillo grapevines at a concentration of 10 mM in 2013, 2014, and 2015 years. Control plants were sprayed with water.

Study of the volatil profile of minority white varieties

The genetic material preservation is a priority issue in winemaking research. The recovery of minority grape varieties can control the genetic erosion, contributing also to preserve wine typical characteristics. In D.O.Ca. Rioja (Spain) the number of grown white varieties has been very limited, representing Viura the 91% of the cultivated white grape area in 2005, while the others, Garnacha Blanca and Malvasía riojana, hardly were grown. For this reason, a recovery and characterization study of plant material was carried out in this region. In 2008, the results obtained allowed the authorization of three minority white varieties: Tempranillo Blanco, Maturana Blanca and Turruntés.

Supramolecular approaches to the study of the astringency elicited by wine phenolic compounds

The objective of this study is to review the scientific evidences and to advance into the knowledge of the molecular mechanisms of astringency. Astringency has been described as the drying, roughing and puckering sensation perceived when some food and beverages are tasted (1). The main, but possibly not the only, mechanism for the astringency is the precipitation of salivary proteins (2,3). Between phenolic compounds found in red wines, flavan-3-ols are the group usually related to the development of this sensation. Other compounds, phenolic or not, like anthocyanins, polysaccharides and mannoproteins could act modifying or modulating astringency perception by hindering the interaction between flavanols and salivary proteins either because of their interaction with the flavanols or because of their interaction with the salivary proteins.

Nitrogen – Lipid Balance in alcoholic fermentations. Example of Champagne musts

Nutrient availability – nitrogen, lipids, vitamins or oxygen – has a major impact on the kinetics of winemaking fermentations. Nitrogen is usually the growth-limiting nutrient and its availability determines the fermentation rate, and therefore the fermentation duration. In some cases, in particular in Champagne, grape musts have high nitrogen concentrations and are sometimes clarified with turbidity below 50 NTU. In these conditions, lipid deficiencies may occur and longer fermentations can be observed. To better understand this situation, a study was realized using a synthetic medium simulating the composition of a Champagne must : 180 g/L of sugar, 360 mg/L of assimilable nitrogen and a lipid content ranging from 1 to 8 mg/L of phytosterols (mainly β-sitosterol).

New acylated flavonols identified in the grape skin of Vitis vinifera cv. Tannat and their wines

Flavonols are a class of flavonoid compounds derived from plant secondary metabolism. There they play different roles like antioxidants, internal regulators and UV screenings. In red wines, flavonols have increasingly received consideration by part of scientific and winemakers according their properties began to arise known. Among these stand out wine colour stabilization and their value as bioactive compounds. In this work the complete series of the acetylated and p-coumaroylated derivatives of the 3-O-glycosides of methoxylated flavonols, namely isorhamnetin, laricitrin and syringetin, have been identified in grapes and their respective wines from Vitis vinifera cv. Tannat.