Macrowine 2021
IVES 9 IVES Conference Series 9 Micro-meteorological, compositional and transcriptional study of corvina grape color during ripening

Micro-meteorological, compositional and transcriptional study of corvina grape color during ripening

Abstract

Grape anthocyanin content and composition could affect the quality and the production strategies of red wines. Differences in the pigment composition modify the color properties in terms of hue, extractability and stability. Thus, for the production of a highly qualitative wine such as “Amarone”, variations in the pigment composition are not negligible. The aim of this work was the investigation of the anthocyanin profile changes during ripening in Corvina grapes, the main cultivar for the “Amarone” production. The experiment took place in 2015, in two vineyards located in Valpollicella (Italy). Micro-meteorological survey, carpological characterization, chemical analysis and transcriptional studies were carried out to clarify the anthocyanin biosynthesis regulation. Air and grape temperatures and global solar radiation inside the canopy where measured during the season. Grape samples were collected at seven stages of berry development from pre-véraison until harvest. Berry growth was followed by both weight and volume increases. At veraison, anthocyanin quantification, by spectroscopy, and profile composition analysis, by HPLC, started (5 developmental stages were analyzed). The expression level of structural and regulatory genes of anthocyanin pathway was studied via real time polymerase chain reaction during all the seven development stages considered. Even if the vineyards are close each other the different training systems (“Guyot” and “Pergola”) created different microclimates for grape ripening. As an example, in “Pergola” the grape temperatures rarely exceeded 40°C. On the opposite, in “Guyot” the temperatures rise over 59°C in hottest days, and often overpassed 50°C. Despite these differences, the anthocyanin profile similarly changed during ripening among the two vineyards. At color appearance (average anthocyanin content: 80 mg kg-1 of grapes), the di-substituted anthocyanins prevailed. During ripening, it was possible to observe a progressive increase of tri-substituted, methoxylated, and acylated pigments. The transcriptional levels of “Guyot” and “Pergola” confirmed to be similar. A clear correlation among expression of anthocyanin biosynthetic gene UDPglucose:flavonoid 3-O-glucosyltransferase (UFGT), transcription factors, MYBA1 and MYBA2, and total anthocyanin content during berry development has been identified. Chalcone synthase, flavonoid 3ʹ-hydroxylase (F3ʹH) and flavonoid 3ʹ,5ʹ-hydroxylase (F3ʹ5ʹH) genes of the flavonoid pathway showed high correlation as well. The proportion changes between tri- and di-substituted anthocyaninswas associated with an increasing ratio of F3ʹ5ʹH/F3ʹH gene transcription during ripening. The AOMT genes were expressed with a maximum at the onset of ripening, coherently with the rapid increase of methoxylated anthocyanin proportion in this stage. Detailed knowledges of grape color variations during ripening could support the vineyard management techniques including harvesting time.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Laura Rustioni*, Federica Zoli, Gabriella De Lorenzis, Lucio Brancadoro, Simone Parisi

*Università di Milano

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Some applications come from a method to concentrate proteins

All techniques usually used to assay proteins was not reliable in vegetable extract due to interferences with the components included in extracts like polyphenols, tanins, pectines, aromatics compounds. Absorbance at 280nm, Kjeldhal assay, Biuret and Lowry methods, Acid Bicinchonique technique and Bradford assay give the results depending on the composition of extract, on the presence or not of detergent and on the raw material (Marchal, 1995). Another difficulty in these extracts for the quantification of proteins comes from the large amount of water included in vegetable and the low concentration of proteins. Thus in red wines, proteins are usually not taken into account due to their low concentration (typically below 10 mgL-1) and to the presence of anthocyanis and polyphenols.

The effect of cropload on the volatile aroma characteristics of ‘Beihong’ and ‘Beimei’ red wine

Beihong and Beimei were bred as winemaking cultivars released by Institute of Botany, the Chinese Academy of Sciences in 2008. The cultivars are selected from the population of ‘Muscat Hamburg’ (Vitis vinifera) ×V. amurensis. They are extended to most provinces in North of China because they have strong resistance to cold and disease and need not be buried in soil in winter. To better understand the effect of cropload on volatile compounds during wine-making, we surveyed volatiles composition and content of different cropload level in 3-years-old ‘Beihong’ and ‘Beimei’ vines which planted in east foot of Helan mountain of Ningxia (EHN).

Multivariate strategies for red wines classification using stilbenes and flavonols content

Bioactive polyphenols from grapes and wines, like stilbenes and flavonols (SaF), are often determined to nutritional evaluation, but also for many other purposes. The objective of this study was to quantify SaF in red wines from “Campanha Gaúcha”, a large and young viticultural region from South Brazil. Moreover, through statistical analysis, evaluate the influence of these compounds according to varieties, production process, harvest years and micro-regions of cultivation. A total of 58 samples of red wines were analyzed by high-performance liquid chromatography coupled to diode array detector (HPLC-DAD) for determination of trans-resveratrol (R), quercetin (Q), myricetin (M), kaempferol (K), trans-e-viniferin (V) and their precursor, cinnamic acid (C).

Study of the content of amino acids and biogenic amines in sparkling red wines

The production of red sparkling wines is lower in Spain in comparison with the winemaking of white or rosé sparkling wines. In red sparkling wine processing it is essential to obtain suitable base wines that should have moderate alcohol content, high acidity, good color values, an adequate mouth-feel and a sweet tannin. Grapes for sparkling wine production have to be harvested at low maturity stages, with lower alcohol contents and higher acidities, which will that the phenolic maturity of the grapes is also low, showing green tannins. This paper analyses different treatments in order to minimize these inconveniences: cold maceration-prefermentation and delestage to elaborate the grapes with lower maturity, must nanofiltration, and the partial osmosis of the wines made from grapes with an adequate maturity degree.

Influence of wood chips addition during alcoholic fermentation on wine phenolic composition

This study investigates the effect of wood chips addition during the alcoholic fermentation on the phenolic
composition of the produced wines. A series of wood chips, originating from American, French, Slavonia
oak and Acacia were added at the beginning of wine alcoholic fermentation. Besides, a mixture consisting
of 50% French and 50% Americal oak chips were added during the experimentation. The wine samples
were analyzed one month after the end of malolactic fermentation, examining various chemical
parameters such as total anthocyanins, total phenolic content, tannins combined with protein (BSA) and
ellagitannin content.