Macrowine 2021
IVES 9 IVES Conference Series 9 Micro-meteorological, compositional and transcriptional study of corvina grape color during ripening

Micro-meteorological, compositional and transcriptional study of corvina grape color during ripening

Abstract

Grape anthocyanin content and composition could affect the quality and the production strategies of red wines. Differences in the pigment composition modify the color properties in terms of hue, extractability and stability. Thus, for the production of a highly qualitative wine such as “Amarone”, variations in the pigment composition are not negligible. The aim of this work was the investigation of the anthocyanin profile changes during ripening in Corvina grapes, the main cultivar for the “Amarone” production. The experiment took place in 2015, in two vineyards located in Valpollicella (Italy). Micro-meteorological survey, carpological characterization, chemical analysis and transcriptional studies were carried out to clarify the anthocyanin biosynthesis regulation. Air and grape temperatures and global solar radiation inside the canopy where measured during the season. Grape samples were collected at seven stages of berry development from pre-véraison until harvest. Berry growth was followed by both weight and volume increases. At veraison, anthocyanin quantification, by spectroscopy, and profile composition analysis, by HPLC, started (5 developmental stages were analyzed). The expression level of structural and regulatory genes of anthocyanin pathway was studied via real time polymerase chain reaction during all the seven development stages considered. Even if the vineyards are close each other the different training systems (“Guyot” and “Pergola”) created different microclimates for grape ripening. As an example, in “Pergola” the grape temperatures rarely exceeded 40°C. On the opposite, in “Guyot” the temperatures rise over 59°C in hottest days, and often overpassed 50°C. Despite these differences, the anthocyanin profile similarly changed during ripening among the two vineyards. At color appearance (average anthocyanin content: 80 mg kg-1 of grapes), the di-substituted anthocyanins prevailed. During ripening, it was possible to observe a progressive increase of tri-substituted, methoxylated, and acylated pigments. The transcriptional levels of “Guyot” and “Pergola” confirmed to be similar. A clear correlation among expression of anthocyanin biosynthetic gene UDPglucose:flavonoid 3-O-glucosyltransferase (UFGT), transcription factors, MYBA1 and MYBA2, and total anthocyanin content during berry development has been identified. Chalcone synthase, flavonoid 3ʹ-hydroxylase (F3ʹH) and flavonoid 3ʹ,5ʹ-hydroxylase (F3ʹ5ʹH) genes of the flavonoid pathway showed high correlation as well. The proportion changes between tri- and di-substituted anthocyaninswas associated with an increasing ratio of F3ʹ5ʹH/F3ʹH gene transcription during ripening. The AOMT genes were expressed with a maximum at the onset of ripening, coherently with the rapid increase of methoxylated anthocyanin proportion in this stage. Detailed knowledges of grape color variations during ripening could support the vineyard management techniques including harvesting time.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Laura Rustioni*, Federica Zoli, Gabriella De Lorenzis, Lucio Brancadoro, Simone Parisi

*Università di Milano

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Comparison of fortified, sfursat and passito winemaking techniques for the enhancement of the oenological potential of the black grape cultivar Moscato nero d’Acqui (Vitis vinifera L.)

One of the key factors of the economical development of viticulture and wine industry in specific limited areas is the exploitation of ancient, local grape varieties. Therefore, in recent years the growing interest to rediscover minor varieties, previously cultivated, has promoted many studies. With this regard, the focus of this study was the Vitis vinifera L. cultivar Moscato nero d’Acqui, nowadays found only in old vineyards in the Acqui zone (North-West Italy). In particular, the aims of this work were: i) to investigate secondary metabolites profile of the grapes, and ii) to evaluate the attitude to the production of special wines.

Accumulation of polyphenols in Barbera and Nebbiolo leaves during the vegetative season

Grapevine berries produce thousands of secondary metabolites of diverse chemical nature that have been largely detailed in the past due to their importance for defining wine quality. The wide Vitis vinifera diversity, resulting in thousands of different varieties well detailed in many studies regarding berries, is still not investigated in vegetative organs, leaves in particular. Deepening knowledge related to this aspect could be of great interest for many reasons (for example the possibility of using leaf extract for pharmaceutical, cosmetic and nutrition purposes) but, above all, for understanding the susceptibility of different grapevine varieties to pathogens.

Effect of malolactic fermentation in barrels or stainless steel tanks on wine composition. Influence of the barrel toasting

Ellagitannin, anthocyanin and woody volatile composition of Cabernet Sauvignon wines aged in oak barrels for 12 months was evaluated. Depending on the container where malolactic fermentation (MLF) was carried out, two wine modalities were investigated: wines with MLF carried out in stainless steel tanks and barrel-fermented wines. Three toasting methods (medium toast, MT; medium toast with watering, MTAA; noisette) were considered for ageing of each wine modality. Sensory analyses (triangle and rating tests) were also performed. Two-way ANOVA of the raw experimental data revealed that the toasting method and the container where MLF took place, as well as the interaction between both factors, have a significant influence (p < 0.05) on ellagitannin, anthocyanin and woody volatile profiles of Cabernet Sauvignon wines.

Use of chitosan as a secondary antioxidant in juices and wines

Chitosan is a polysaccharide produced from the deacetylation of chitin extracted from crustaceous and fungi. In winemaking chitosan is mainly used in the clarification of grape juice and wine, stabilization of white wines, removal of metals and to prevent wine spoilage by undesired microorganisms. The addition of chitosan to model wine systems was able to retard browning, reduce levels of metallic ions (Fe and Cu) and to protect varietal thiols due to its antiradical activity1. The present experiment was planned in order to evaluate the use of chitosan as a secondary antioxidant at three different stages of Sauvignon blanc fermentation and winemaking. Sauvignon blanc juices from three different locations were obtained at a commercial winery in Marlborough, New Zealand. One lots of grapes was collected from a receival bin and pressed into juice with a water-bag press, and a further juice sample was collected from a commercial pressing operation. Chitosan (1 g/L, low molecular weight, 75 – 85% deacetylated) was added to the juice after pressing, after cold settling, after fermentation, or at all these stages. Controls without any chitosan additions were also prepared.

Cover crops influence on soil N availability and grapevine N status, and its relationship with biogenic

The type of soil management, tillage versus cover crops, can modify the soil microbial activity, which causes the mineralization of organic N to NO3–N and, therefore, may change the soil NO3–N availability in vineyard. The soil NO3–N availability could influence the grapevine nutritional status and the grape amino acid composition. Amino acids are precursors of biogenic amines, compounds mainly formed during the malolactic fermentation. Biogenic amines have negative effects on consumer health and on the wine organoleptic quality. The objective was to study if the effect of conventional tillage and two different cover crops (leguminous versus gramineous) on grapevine N status, could relate to the wine biogenic amines composition.