Macrowine 2021
IVES 9 IVES Conference Series 9 Micro-meteorological, compositional and transcriptional study of corvina grape color during ripening

Micro-meteorological, compositional and transcriptional study of corvina grape color during ripening

Abstract

Grape anthocyanin content and composition could affect the quality and the production strategies of red wines. Differences in the pigment composition modify the color properties in terms of hue, extractability and stability. Thus, for the production of a highly qualitative wine such as “Amarone”, variations in the pigment composition are not negligible. The aim of this work was the investigation of the anthocyanin profile changes during ripening in Corvina grapes, the main cultivar for the “Amarone” production. The experiment took place in 2015, in two vineyards located in Valpollicella (Italy). Micro-meteorological survey, carpological characterization, chemical analysis and transcriptional studies were carried out to clarify the anthocyanin biosynthesis regulation. Air and grape temperatures and global solar radiation inside the canopy where measured during the season. Grape samples were collected at seven stages of berry development from pre-véraison until harvest. Berry growth was followed by both weight and volume increases. At veraison, anthocyanin quantification, by spectroscopy, and profile composition analysis, by HPLC, started (5 developmental stages were analyzed). The expression level of structural and regulatory genes of anthocyanin pathway was studied via real time polymerase chain reaction during all the seven development stages considered. Even if the vineyards are close each other the different training systems (“Guyot” and “Pergola”) created different microclimates for grape ripening. As an example, in “Pergola” the grape temperatures rarely exceeded 40°C. On the opposite, in “Guyot” the temperatures rise over 59°C in hottest days, and often overpassed 50°C. Despite these differences, the anthocyanin profile similarly changed during ripening among the two vineyards. At color appearance (average anthocyanin content: 80 mg kg-1 of grapes), the di-substituted anthocyanins prevailed. During ripening, it was possible to observe a progressive increase of tri-substituted, methoxylated, and acylated pigments. The transcriptional levels of “Guyot” and “Pergola” confirmed to be similar. A clear correlation among expression of anthocyanin biosynthetic gene UDPglucose:flavonoid 3-O-glucosyltransferase (UFGT), transcription factors, MYBA1 and MYBA2, and total anthocyanin content during berry development has been identified. Chalcone synthase, flavonoid 3ʹ-hydroxylase (F3ʹH) and flavonoid 3ʹ,5ʹ-hydroxylase (F3ʹ5ʹH) genes of the flavonoid pathway showed high correlation as well. The proportion changes between tri- and di-substituted anthocyaninswas associated with an increasing ratio of F3ʹ5ʹH/F3ʹH gene transcription during ripening. The AOMT genes were expressed with a maximum at the onset of ripening, coherently with the rapid increase of methoxylated anthocyanin proportion in this stage. Detailed knowledges of grape color variations during ripening could support the vineyard management techniques including harvesting time.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Laura Rustioni*, Federica Zoli, Gabriella De Lorenzis, Lucio Brancadoro, Simone Parisi

*Università di Milano

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

A multivariate approach using attenuated total reflectance mid-infrared spectroscopy to measure the surface mannoproteins and β-glucans of yeast cell walls during wine fermentations

Yeast cells possess a cell wall comprising primarily glycoproteins, mannans, and glucan polymers. Several yeast phenotypes relevant for fermentation, wine processing, and wine quality are correlated with cell wall properties. To investigate the effect of wine fermentation on cell wall composition, a study was performed using mid-infrared (MIR) spectroscopy coupled with multivariate methods (i.e., PCA and OPLS-DA). A total of 40 yeast strains were evaluated, including Saccharomyces strains (laboratory and industrial) and non-Saccharomyces species. Cells were fermented in both synthetic MS300 and Chardonnay grape must to stationery phase, processed, and scanned in the MIR spectrum.

Screening sensory-directed methodology for the selection of non-saccharomyces wine yeasts based on perceived aroma quality

The present work contributes by developing a rapid sensory-directed methodology for the screening and selection of high quality wines with different sensory profiles Therefore, Verdejo and Tempranillo musts were fermented with 50 different yeasts each under controlled laboratory conditions. Resulting samples were firstly categorized according to five levels of quality by a panel of wine professionals (Sáenz-Navajas, Ballester et al. 2013). Higher quality samples were described by flash profiling by a semi-trained panel
(Valentin, Chollet et al. 2012) and most distinctive samples were screened by gas chromatography-olfactometry (GC-O) (López, Aznar et al. 2002).

Identification of green, aggressive and hard character of wines by a chemo-sensory directed methodology

With climate change, it is progressively more often to obtain grapes with an acceptable content in sugars or acids but with immature tannins described as green, aggressive or hard (noted as GAH onwards). During winemaking, the oenologist has to make decisions related to the elaboration of such grapes based mainly on empirical experience, given the lack of objective criteria to this concern. An increase in the chemical and sensory knowledge of immature tannins would allow managing this GAH character of grapes with the maximum possible efficiency during winemaking processes. The present work aims at isolating and identifying the group of compounds responsible for the GAH character present in wines.

Effect of nanofiltration on the chemical composition and wine quality

In Enology the conventional processes of filtration for clarification and stabilization are giving place to alternative membrane processes, including nanofiltration (NF). Furthermore, the increased alcohol content in wines recorded in recent years became an important issue for all the main wine producing countries. Among techniques available to the wine industry to reduce the ethanol content, NF is certainly one of the newest. This study is focused on the evaluation of NF influence on wine physical-chemical composition, including mineral content, which in accordance to our best knowledge is a novelty.

How pressing techniques affect must composition and wine quality of Pinot blanc

This study investigates how the sensory profile of Pinot Blanc is affected from different maceration and pressing techniques. Grapes were sourced from four vineyards in the village Tramin in South Tyrol. For the experiment 200 kg of grapes from each vineyard site were hand picked the day before harvest for the commercial winery took place. Grapes were stored over night at 4°C, homogenized and processed in the experimental winery at Laimburg research centre the day after harvest. Four different pressing techniques were applied in duplicates of 100kg each.