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IVES 9 IVES Conference Series 9 Raffinose: a sweet solution for grapevine drought tolerance

Raffinose: a sweet solution for grapevine drought tolerance

Abstract

Water tolerance in plants is often associated with the accumulation of osmotic protectants, which are secondary metabolites that can help the plant to cope with water stress. One of the key osmotic protectants is a sugar called Raffinose, which is synthesized by a family of enzymes called Raffinose synthases. In this work, we focused on one of these enzymes, VviRAF2, which is a gene that shows different expression levels and genetic variants (SNPs) among different grapevine cultivars, ranging from tolerant to susceptible to water stress, and the transcription factors that may regulate the expression of this gene family. We analyzed the transcriptome data of these cultivars and constructed a gene co-expression network based on the reference genome, which revealed the involvement of the MYB transcription factor named ‘AQUILO’. To test the function of VviRAF2 and ‘AQUILO’ in water-stress tolerance, we engineered such genes via Agrobacterium tumefaciens using both, transgenic and cisgenic approach: one VviRAF2 under the control of the 35-s promoter, and another with the insertion of AQUILO controlled by its own promoter. During this study, we performed gene expression experiments on transformed lines to compare the DEGs in response to water-stress. Finally, we present the preliminary results related to stress response underlying the pathways of water stress tolerance.  

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Álvaro Vidal Valenzuela1,2,3,4*, José Tomás Matus2, David Navarro-Paya2,Felipe Gainza-Cortés3, Maria Stella Grando4, Olivier Zekri5, Pierre Videau5, Katerina Labonova5, Lorenza Dalla Costa1, Mickael Malnoy1

1 Research and Innovation centre, Fondazione Edmund Mach, Via Mach 1, 38098 San michelle all’adige(TN), Italy
2 Institute for Integrative Systems Biology (I2SysBio), Universitat de València-CSIC, Paterna, 46980, Valencia, Spain
3 Center for Research and Innovation (CII), Viña Concha y Toro, 3550000, Pencahue, Chile
4 Center Agriculture Food Environment (C3A), University of Trento, via E Mach 1, 38010 San Michele all’Adige, Italy
5 Mercier Novatech, Le Champ des Noëls, 85770 – Le gué de velluire, France

Contact the author*

Keywords

Raffinose, Metabolites, Drought, Abiotic stress, sugar

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

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