terclim by ICS banner
IVES 9 IVES Conference Series 9 Raffinose: a sweet solution for grapevine drought tolerance

Raffinose: a sweet solution for grapevine drought tolerance

Abstract

Water tolerance in plants is often associated with the accumulation of osmotic protectants, which are secondary metabolites that can help the plant to cope with water stress. One of the key osmotic protectants is a sugar called Raffinose, which is synthesized by a family of enzymes called Raffinose synthases. In this work, we focused on one of these enzymes, VviRAF2, which is a gene that shows different expression levels and genetic variants (SNPs) among different grapevine cultivars, ranging from tolerant to susceptible to water stress, and the transcription factors that may regulate the expression of this gene family. We analyzed the transcriptome data of these cultivars and constructed a gene co-expression network based on the reference genome, which revealed the involvement of the MYB transcription factor named ‘AQUILO’. To test the function of VviRAF2 and ‘AQUILO’ in water-stress tolerance, we engineered such genes via Agrobacterium tumefaciens using both, transgenic and cisgenic approach: one VviRAF2 under the control of the 35-s promoter, and another with the insertion of AQUILO controlled by its own promoter. During this study, we performed gene expression experiments on transformed lines to compare the DEGs in response to water-stress. Finally, we present the preliminary results related to stress response underlying the pathways of water stress tolerance.  

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Álvaro Vidal Valenzuela1,2,3,4*, José Tomás Matus2, David Navarro-Paya2,Felipe Gainza-Cortés3, Maria Stella Grando4, Olivier Zekri5, Pierre Videau5, Katerina Labonova5, Lorenza Dalla Costa1, Mickael Malnoy1

1 Research and Innovation centre, Fondazione Edmund Mach, Via Mach 1, 38098 San michelle all’adige(TN), Italy
2 Institute for Integrative Systems Biology (I2SysBio), Universitat de València-CSIC, Paterna, 46980, Valencia, Spain
3 Center for Research and Innovation (CII), Viña Concha y Toro, 3550000, Pencahue, Chile
4 Center Agriculture Food Environment (C3A), University of Trento, via E Mach 1, 38010 San Michele all’Adige, Italy
5 Mercier Novatech, Le Champ des Noëls, 85770 – Le gué de velluire, France

Contact the author*

Keywords

Raffinose, Metabolites, Drought, Abiotic stress, sugar

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Phenology, thermal requirements and maturation of the SR 0.501-17 wine grape hybrid cultivated in contrasting climate

The use of hybrids in viticulture is one of the alternatives for sustainable production in hot and rainy regions during grapevine maturation. This sustainable production concerns the reduction of pesticide use, adaptation to climate and control of vine decline. The SR 0.501-17 wine grape hybrid, developed in the grapevine program of the Agronomic Institute of Campinas (IAC), is characterized by producing white grapes with small spherical berries with seeds. The agronomic characterization of this hybrid, especially in different climatic conditions, as well as the evaluation of its performance in winemaking are necessary. The objective of this work was to characterize the duration and thermal requirements of the different phenological stages and the influence of rainfall on the physicochemical characteristics of the must in two contrasting climate regions of the State of São Paulo.

La certificazione ambientale del territorio: fattibilita’ e prospettive

In the next years the territorial environmental certification could become realistic if the following conditions will be fully satisfied:
– the enhancement of the environmental awareness among the industries, the public administration, the authorization bodies, the living people of that territory as well as the tourists and visitors.

White wine light-strike fault: a comparison between flint and green bottles under the typical supermarket conditions

Consumer preference favors flint-glass wine bottles over the traditional dark-colored, but it is documented that light exposure can cause white wines to produce off-aromas and change in color, and consequently da[1]mage their quality. Aim of the study was to study the white wine shelf life under the typical supermarket conditions, by recording the light and temperature exposure, the colorimetric changes, and the light-strike fault. METHODS: One pilot experiment based on two white wines and eight-time points and one kinetic experiment based on four white wines and seven-time points were designed and realized using a typical supermarket shelf for 32 and 50 days, correspondently. By installing prototype sensors at 32 points of the shelf, the temperature, UV, IR, and Visible light exposure were registered every 10 min. Approximately 600 commercial wines, bottled in flint and colored glass, were used. The colorimetric changes of the wines were registered and the light-strike fault was evaluated.

Management of cover plants impacted the composition of Cabernet Sauvignon red wines in a temperate region of Brazil

– Several practices can be applied to vineyards in order to ensure good healthy for grapevines, adequate yield and fruit quality. Among them, the use of cover crops is a relevant option for soil management. It increases the organic matter, improves water infiltration, reduces risks of soil erosion and greenhouse gas emissions, in addition improving biodiversity in the vineyard.

Influence of canopy management on yield, grape and wine quality. Relationship between the potassium content and pH in must and wine of the cultivar “Tempranillo”

In recent years red wines are being produced in Andalusia from indigenous and foreign grape varieties, one of which is the Spanish variety Tempranillo.