Pedological factor influence on the viticultural zoning of the Aljarafe Alto (Seville, Spain)

Climate transition with frequent heat waves and long drought periods threatens grapevine productivity and wine quality in the Mediterranean regions. Microorganisms are known to contribute to plant fitness and to stimulate plant resilience against biotic and abiotic factors.
In this work, it was assessed the impact of long-term drought on soil microbiome associated to grapevine in open field in Alentejo, renowned Portuguese wine region.
Soil and plant tissues of drought tolerant Syrah cultivar exposed to three irrigation levels (100%- FI, 50%-DI ETc; rain-fed–NI) for 5 years were sampled for two years (2022-2023). Metabarcoding analysis of soil bacteria (16S V4 rRNA) and fungi (ITS sub-region) were integrated with soil physiochemical properties and leaves´ physiological data. Pre-dawn leaf water potential and stomatal conductance confirmed the imposed drought scenarios. Even though, α- and β-diversity of prokaryotic and eukaryotic microbial communities differed more by season than water availability, samples clustered according to soil water content and pH (p<0.05). Fungal communities show higher differences in the structure across treatments than bacteria. In 2023, 16 bacterial against 61 fungal ASVs were significatively different in abundance between NI and FI. Beijerinckiaceae, Bradyrhizobiaceae (Alphaproteobacteria) and Nocardioidaceae, Streptomycetaceae (Actinobacteria) families resulted to be significatively more abundant in NI, while Ascomycota, Basidyomicota and Mortierellomycota are the most important fungal phyla in NI. With culturomics data, this study aims to gather insights into how soil microbiome is remodelled under drought and contribute to select bacterial and fungal taxa with potential to mitigate drought stress in vineyards.

Vine water use is a critical determinant of vineyard management practices, especially in the context of climate change.

Viticulture is facing many challenges due to climate change effects with increasingly attention to save resources, such as water, considering that drought events have been predicted to dramatically increase over the next future. Thanks to the -omics techniques, research pushed forward knowledge to deepen facets of drought response in diverse grapevine-rootstock combinations. However, the regulatory mechanisms orchestrating adaptation strategies during drought and recovery in grafted grapevines need further exploration. Herein, we combined ecophysiological, biochemical and molecular approaches to unravel drought and recovery-induced changes in potted Nebbiolo (NE) plants grafted onto three different rootstocks (3309, Kober5BB, Gravesac), by analysing root and leaf tissues.

The analysis of consumer attitudes towards wine, especially towards wines perceived as faulty, is an aspect that requires more research than has been carried out so far [1]. This study aims to analyse consumer behaviour in situations involving the consumption of faulty wines and to assess the level of acceptance of such wines.

There is a growing interest in the exploitation of vine-shoots waste, since they are often left or burned. Sánchez-Gómez et al. [1] have shown that vines-shoots aqueous extracts have significant contents of bioactive compounds, among which several polyphenols and volatiles are highlighted. Recent studied had demonstrated that the chemical composition of vine-shoots is enhanced when vine-shoots are toasted
[2,3]. The application of vegetable products in the vineyards has led to significant changes towards a more “Sustainable Viticulture”. An innovative foliar application for Airén vine-shoot extracts have been carried out to the vineyard. It has been shown that they act as grape biostimulants, improving certain wine quality characteristics [4].