terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Effect of pH and ethanol on Lactiplantibacillus plantarum in red must fermentation: potential use of wine lees

Effect of pH and ethanol on Lactiplantibacillus plantarum in red must fermentation: potential use of wine lees

Abstract

Wine is the result of the alcoholic fermentation (AF) of grape must. Besides AF, wine can also undergo the malolactic fermentation (MLF) driven out by lactic acid bacteria (LAB). Among LAB, Oenococcus oeni and Lactiplantibacillus plantarum are the dominant species in wine. Even if O. oeni is the most common LAB undergoing MLF in wine, due to its high tolerance to wine conditions, L. plantarum can be used to undergo MLF in must. The moderate tolerance of L. plantarum to low pH and ethanol, may compromise the fermentative process in harsh wines. Besides, the use of wine lees has been reported to stimulate MLF of O. oeni in wine [1]. Altogether, the aim of this work was to evaluate the MLF performance of L. plantarum in red grape must at different pHs, ethanol concentrations, and in the presence or absence of wine lees.

The results showed that L. plantarum can perform MLF even at pH 2.5. However, in presence of 6% (vol/vol) of ethanol, which can correspond to an early AF stage, MLF failed below pH 3.25. This behavior was observed in two commercial strains. However, the presence of wine lees in grape must at pH 3.25 allowed the completion of MLF. The addition of wine lees to the preinoculum or to the grape must also enhanced bacterial survival. Indeed, the supplementation in the preinoculum allowed the depletion of L-malic acid with one strain. In summary, wine lees potentially increase the survival of L. plantarum in red grape must, and thus, enhance MLF performance.

Acknowledgements: this work was supported by grant PID2021-124943OB-I00 (Spanish Research Agency). Aitor Balmaseda is a Margarita Salas postdoc researcher (2021URVMS25, Spanish Ministry of Universities financed with European Union-NextGenerationEU).

References:

1)  Balmaseda A. et al. (2021). Simulated lees of different yeast species modify the performance of malolactic fermentation by Oenococcus oeni in wine-like medium. Food Microbiology, 99, 103839.

DOI:

Publication date: October 10, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Aitor Balmaseda1, *, Albert Bordons1, Nicolas Rozès2, Cristina Reguant1

1Universitat Rovira i Virgili, Departament de Bioquímica i Biotecnologia, Facultat d’Enologia, Grup de Biotecnologia Enològica, C/ Marcellí Domingo 1, 43007 Tarragona, Catalonia, Spain
2Universitat Rovira i Virgili, Departament de Bioquímica i Biotecnologia, Facultat d’Enologia, Grup de Biotecnologia Microbiana dels Aliments, C/ Marcellí Domingo 1, 43007 Tarragona, Catalonia, Spain

Contact the author*

Keywords

Lactiplantibacillus plantarum, malolactic fermentation, wine lees, must

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Long-lasting flavour perception of wines treated with oenological additives considering the individual PROP taste-phenotype

The use of oenological additives is becoming a common practice due to the technological and sensory properties they provide to the wines. However, the number of studies focused on the impact that these additives might induce on wine flavor perception during wine tasting is still quite scarce. The aim of this work was to evaluate the effect of three different types of common oenological additives: two oenotannins (ellagitannin and gallotannin) and a commercial preparation of yeast mannoproteins on the long-lasting flavor perception (aroma and astringency).

Grape pomace, an active ingredient at the intestinal level: Updated evidence

Grape pomace (GP) is a winemaking by-product particularly rich in (poly)phenols and dietary fiber, which are the main active compounds responsible for its health-promoting effects. GP-derived products have been proposed to manage cardiovascular risk factors, including endothelial dysfunction, inflammation, hypertension, hyperglycemia, and obesity. Studies on the potential impact of GP on gut health are much more recent. However, it is suggested that, to some extent, this activity of GP as a cardiometabolic health-promoting ingredient would begin in the gastrointestinal tract as GP components (i.e., (poly)phenols and fiber) undergo extensive catabolism, mainly by the action of the intestinal microbiota, that gives rise to low-molecular-weight bioactive compounds that can be absorbed and utilized by the body.

Survey of pesticide residues in vineyard soils from the Denomination of Origin Ribeiro

Vineyards from mild temperature, high humidity locations receive often treatments with fungicides to prevent damages produced by fungi responsible for mildium, oidium and botrytis infections. In addition, insecticides are also applied to vineyards to fight again pests, which affect directly, or indirectly (as vectors of different diseases), their productivity. A fraction of the above compounds reaches the soil of vineyards, either during application, or when released from the canopy of vines due to rain-wash-off. Thereafter, depending on soil conditions (pH, organic matter) and environmental variables (regimen of rain, slope of vineyards), they might persist in this compartment, be degraded and/or transferred to water masses, modifying the biodiversity of soils and/or affecting the quality of water reservoirs.

Effect of spray with autochthonous Trichoderma strains and its secondary metabolites on the quality of Tempranillo grape

Trichoderma is one of the most widely used fungal biocontrol agents on vineyards due to its multiple benefits on this crop, such as its fungicidal and growth promoting capacity. In this work, we have analyzed the effect on the concentration of nutrients in grapevine leaves and on the quality of the grape must after spraying an autochthonous strain of Trichoderma harzianum and one of the main secondary metabolites produced by this genus, 6-pentyl-α-pyrone (6PP).

First results on the chemical composition of red wines from the pressing of marc

In the Bordeaux vineyards, press wine represents approximately 15% of the total volume of wine produced[1]. Valuing this large volume of wine is necessary from an economic point of view, but also because of their organoleptic contribution to the blend, and their contribution to the construction of wines for laying down. Therefore, this study was developed considering the lack of recent scientific knowledge on the composition of red press wines. The aim of this study is to establish an initial assessment of their chemical composition including aromatic compounds and a phenolic part.