Macrowine 2021
IVES 9 IVES Conference Series 9 Micro-meteorological, compositional and transcriptional study of corvina grape color during ripening

Micro-meteorological, compositional and transcriptional study of corvina grape color during ripening

Abstract

Grape anthocyanin content and composition could affect the quality and the production strategies of red wines. Differences in the pigment composition modify the color properties in terms of hue, extractability and stability. Thus, for the production of a highly qualitative wine such as “Amarone”, variations in the pigment composition are not negligible. The aim of this work was the investigation of the anthocyanin profile changes during ripening in Corvina grapes, the main cultivar for the “Amarone” production. The experiment took place in 2015, in two vineyards located in Valpollicella (Italy). Micro-meteorological survey, carpological characterization, chemical analysis and transcriptional studies were carried out to clarify the anthocyanin biosynthesis regulation. Air and grape temperatures and global solar radiation inside the canopy where measured during the season. Grape samples were collected at seven stages of berry development from pre-véraison until harvest. Berry growth was followed by both weight and volume increases. At veraison, anthocyanin quantification, by spectroscopy, and profile composition analysis, by HPLC, started (5 developmental stages were analyzed). The expression level of structural and regulatory genes of anthocyanin pathway was studied via real time polymerase chain reaction during all the seven development stages considered. Even if the vineyards are close each other the different training systems (“Guyot” and “Pergola”) created different microclimates for grape ripening. As an example, in “Pergola” the grape temperatures rarely exceeded 40°C. On the opposite, in “Guyot” the temperatures rise over 59°C in hottest days, and often overpassed 50°C. Despite these differences, the anthocyanin profile similarly changed during ripening among the two vineyards. At color appearance (average anthocyanin content: 80 mg kg-1 of grapes), the di-substituted anthocyanins prevailed. During ripening, it was possible to observe a progressive increase of tri-substituted, methoxylated, and acylated pigments. The transcriptional levels of “Guyot” and “Pergola” confirmed to be similar. A clear correlation among expression of anthocyanin biosynthetic gene UDPglucose:flavonoid 3-O-glucosyltransferase (UFGT), transcription factors, MYBA1 and MYBA2, and total anthocyanin content during berry development has been identified. Chalcone synthase, flavonoid 3ʹ-hydroxylase (F3ʹH) and flavonoid 3ʹ,5ʹ-hydroxylase (F3ʹ5ʹH) genes of the flavonoid pathway showed high correlation as well. The proportion changes between tri- and di-substituted anthocyaninswas associated with an increasing ratio of F3ʹ5ʹH/F3ʹH gene transcription during ripening. The AOMT genes were expressed with a maximum at the onset of ripening, coherently with the rapid increase of methoxylated anthocyanin proportion in this stage. Detailed knowledges of grape color variations during ripening could support the vineyard management techniques including harvesting time.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Laura Rustioni*, Federica Zoli, Gabriella De Lorenzis, Lucio Brancadoro, Simone Parisi

*Università di Milano

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

DNA and type of grain: which factor does better explain sensory differences of sessile and pedunculate oaks?

Sessile oak and pedunculate oak have shown several differences of interest for enological purposes. Tannic and aromatic composition among sessile oak or pedonculate oak has been well studied. Sessile oak is generally more aromatic than pedunculated, while the later is more tannic. This scientific point of view is rarely applied to classify oak in cooperages. Most coopers use the type of grain to distinguish wide and thin grain.

Comprehensive exploration of wine aroma-related compounds as promoted by alternative vinification procedures in case of Zelen (Vitis vinifera L.) grapes processing

Not only vintner’s decisions in the vineyard, but also winemaker’s choices of technology approaches in the cellar play a significant role in the final wine style and quality. Whereas traditional technologies within chosen terroir are quite well explored and thus somehow predictable, there is no proper knowledge available on possible outcomes in case of implementing novel, alternative winemaking strategies. To reveal their effects on wine aroma compounds and sensory characteristics, two alternative strategies
(cryoextraction or addition of whole grape berries during last stages of fermentation) were compared to classical Vipava valley winemaking approach as normally used for an autochthonous variety Zelen. After separate vinification and bottling, all the experimental wines were subjected to semiquantitative metabolic profiling of volatile compounds (VOCs) by means of GC/MS and were then also sensorialy evaluated by pre-trained panel.

Identification of green, aggressive and hard character of wines by a chemo-sensory directed methodology

With climate change, it is progressively more often to obtain grapes with an acceptable content in sugars or acids but with immature tannins described as green, aggressive or hard (noted as GAH onwards). During winemaking, the oenologist has to make decisions related to the elaboration of such grapes based mainly on empirical experience, given the lack of objective criteria to this concern. An increase in the chemical and sensory knowledge of immature tannins would allow managing this GAH character of grapes with the maximum possible efficiency during winemaking processes. The present work aims at isolating and identifying the group of compounds responsible for the GAH character present in wines.

New biological tools to control and secure malolactic fermentation in high pH wines

Originally, the role of the malolactic fermentation (MLF) was simply to improve the microbial stability of wine via biological deacidification. However, there is an accumulation of evidence to support the fact that lactic acid bacteria (LAB) also contribute positively to the taste and aroma of wine. Many different LAB enter into grape juice and wine from the surface of grape berries, cluster stems, vine leaves, soil and winery equipment. Due to the highly selective environment of juices and wine, only a few types of LAB are able to grow.

Impact of smoke exposure on the chemical composition of grapes

Vineyard exposure to smoke can lead to grapes and wine which exhibit objectionable smoky and ashy aromas and flavours, more commonly known as ‘smoke taint’ [1, 2]. In the last decade, significant bushfires have occurred around the world, including near wine regions in Australia, Canada, South Africa and the USA, as a consequence of the warmer, drier conditions associated with climate change. Considerable research has subsequently been undertaken to determine the chemical, sensory and physiological consequences of grapevine exposure to smoke. The sensory attributes associated with smoke-tainted wine have been linked to the presence of several smoke-derived volatile phenols, such as guaiacols, syringols and cresols [2].