terclim by ICS banner
IVES 9 IVES Conference Series 9 Exploring diversity of grapevine responses to Flavescence dorée infection

Exploring diversity of grapevine responses to Flavescence dorée infection

Abstract

Flavescence dorée, a serious threat to grapevine cultivation in several European Countries, is caused by phytoplasmas in the 16Sr-V ribosomal group, classified as quarantine organisms in the EU and transmitted mainly by the insect vector Scaphoideus titanus. The disease is controlled only by indirect and preventive measures, with important economic and environmental concerns. Genetic resources from the great variety of Vitis vinifera germplasm together with application of new genomic techniques could be applied to produce resistant/tolerant plants, once the genetic bases of susceptibility are elucidated. In a current Italian project (BIORES*) we are evaluating different international and local grapevine cvs. as well as microvine plants for their response to FD transmission and multiplication in controlled conditions. Infective vectors were caged onto potted plants of 8 varieties for 5 weeks. Insect survival and infectivity were monitored and plant infections were quantitatively tested 11 weeks post inoculation.
In parallel, previous data and materials generated by the Project Consortium are being mined to select putative resistance- or susceptibility-related genes, to be further validated in functional analyses, either on already produced transgenic plants or in future genome editing experiments to introduce precise targeted mutations in candidate genes of the highly susceptible cv. Chardonnay, with the final goal of reducing FD susceptibility without compromising other productive traits.

*Funded by the European Union – NextGenerationEU.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Francesca Cavagna1, Enea Guerrieri1, Davide Danzi1, Sabrina Palmano2, Cristina Marzachì2, Nicola Mori1, Annalisa Polverari1*

1 Department of Biotechnology, University of Verona, via della Pieve 70, San Pietro in Cariano, Verona, Italy
2 Institute for Sustainable Plant Protection, CNR, Strada delle Cacce 73, 10135 Torino, Italy

Contact the author*

Keywords

Phytoplasma, Germplasm, Breeding, Genome editing, Sustainable viticulture

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

A multilayer interactive web map of the wine growing region carnuntum with emphasis on geochemical and mineralogical zoning

During a three-year study the vineyards of the wine-growing region Carnuntum have been investigated for their terroir characteristics (climate, soil, rocks) and major viticulture functions. As an outcome of the study, various thematic layers and geodata analyses describe the geo-environmental properties and variability of the wine growing region and delimit homogenous multilayer mapping units by using a Geographic Information System.

Zoning influence in chromatic parameters in Monastrell grape

Zoning analysis determine homogeneous areas principally from the point of view of the medium, giving as a result a map which cartographic units synthesize the relations between the edaphic factors; morphological factors of the soil and climatic factors

On the losses of dissolved CO2 during champagne aging

A misconception lingers in the minds of some wine consumers that Champagne wines don’t age. It’s largely a myth, certainly as far as the best cuvees are concerned. Actually, during the so-called autolysis period of time (in the closed bottle, after the “prise de mousse”), complex chemical reactions take place when the wine remains in contact with the dead yeast cells, which progressively bring complex and very much sought-after aromas to champagne. Nevertheless, despite their remarkable impermeability to liquid and air, caps or natural cork stoppers used to cork the bottles are not 100% hermetic with regard to gas transfers. Gas species therefore very slowly diffuse through the cap or cork stopper, along their respective inverse partial pressure. After the “prise de mousse”, because the partial pressure of CO2 in the bottleneck reaches up to 6 bars (at 12 °C), gaseous CO2 progressively diffuse from the bottle to the ambient air
(where the partial pressure of gaseous CO2 is only of order of 0,0004 bar).

Genetic determinism of grapevine development stages as a tool for the adaptation to climate change

A major goal of modern grapevine (Vitis vinifera L.) breeding programs is the introgression of resistance genes along with desirable traits for better adaptation to climate change. Developmental stages have an impact on yield components and berry composition and are expected to shift towards earlier dates in the future. We investigated the genetic determinism of phenological stages in the progeny of a cross between two grapevine hybrids, each carrying several quantitative trait loci (QTL) for downy mildew and powdery mildew resistance.

INFLUENCE OF GRAPE RIPENESS ON MACROMOLECULES EXTRACTABILITY FROM GRAPE SKIN TISSUES AND GRAPE SEEDS DURING WINEMAKING

A consequence of climate change is the modification of grape harvest quality and physico-chemical parameters of the obtained wine: increase in alcoholic degree, decrease in pH, and modification of the extractability of macromolecules, which leads to problems of microbiological, tartaric, colour and colloidal stability. In order to respond to these problems, the winemaking processes must be anticipated and adapted with a better knowledge of macromolecule extractability in grapes and their evolution, according to the grape variety, vintage and winemaking process. The purpose of this study was to understand 1) how the harvest date can influence the extractability of macromolecules, polysaccharides and phenolic compounds, which are responsible for wine stability 2) how to adapt the winemaking process to the harvest date in order to optimise wine quality.