Vine responses to two irrigation systems in the region of Vinhos Verdes

Polyphenols, bioactive secondary metabolites abundantly found in various grapevine components such as stalks, skins, and seeds, have attracted considerable attention in recent decades due to their potential health benefits. These compounds, including flavan-3-ols, flavanols, flavones, and stilbenes, are known for their antioxidant and anti-inflammatory properties.

Dans des travaux précédents sur le zonage, on a traité de la « Grande Filière », du « terroir », du « territoire », de la «″Terra »″ (« Terre »”), des « Petits zonages ou sub-zonages », du « Grand Zonage », de la qualité (nous en avons classifié plus de quatre-vingt-dix), des « Grands Objectifs » (GO) de l’activité vitivinicole et des moyens utilisés pour les atteindre. Dans le « GRAND ZONAGE » (GZ) nous avons précisé que pour zoner, nous partons des aspects

The Champagne vine-growing region has played a pioneering role in the delimitation of appellations of origin (AOC). The implementation of the Act of July, 22nd 1927 has led to drawing up lists of vine plots based on the criterion of vine cultivation antecedence.

Valbelluna valley is an area located in the northeastern Italy. It is extended from the East-West between Feltre and Belluno, along the Piave waterway and enclosed between Cansiglio valley on the South and the Dolomites in the North. Here, the villages of Limana and Trichiana are present, which are considered for decades potentially interesting areas to aim a niche production with own particular properties.The position of this area, its sun exposition, its soil composition and the microclimate, are ideal factors to obtain vines and consequently wines with unique features especially regarding the diversity and complexity aroma.

Obtaining high-quality RNA from grape tissues, including berry pulp, berry skins, stems, rachis, or roots, is challenging due to their composition, which includes polysaccharides, phenolic compounds, sugars, and organic acids that can negatively affect RNA extraction. For instance, polyphenols and other secondary metabolites can bind to RNA, making it difficult to extract a pure sample. Additionally, RNA can co-precipitate with polysaccharides, leading to lower extraction yield. Also, sugars and organic acids can interfere with the pH and ionic properties of the extraction buffer. To address these challenges, we optimized a protocol for RNA isolation from grape tissues.