terclim by ICS banner
IVES 9 IVES Conference Series 9 Impact on leaf morphology of Vitis vinifera L. cvs Riesling and Cabernet Sauvignon under Free Air Carbon dioxide Enrichment (FACE)

Impact on leaf morphology of Vitis vinifera L. cvs Riesling and Cabernet Sauvignon under Free Air Carbon dioxide Enrichment (FACE)

Abstract

Atmospheric carbon dioxide (CO2) concentration has continuously increased since pre-industrial times from 280 ppm in 1750, and is predicted to exceed 700 ppm by the end of 21st century. For most of C3 plant species elevated CO2 (eCO2) improve photosynthetic apparatus results in an increased plant biomass production. To investigate the effects of eCO2 on morphological leaf characteristics the two Vitis vinifera L. cultivars, Riesling and Cabernet Sauvignon, grown in the Geisenheim VineyardFACE (Free Air Carbon dioxide Enrichment) system were used. The FACE site is located at Geisenheim University (49° 59′ N, 7° 57′ E, 94 m above sea level), Germany and was implemented in 2014 comparing future atmospheric CO2-concentrations (eCO2, predicted for the mid-21st century) with current ambient CO2-conditions (aCO2). Experiments were conducted under rain-fed conditions for two consecutive years (2015 and 2016). Six leaves per repetition of the CO2 treatment were sampled in the field and immediately fixed in a FAA solution (ethanol, H2O, formaldehyde and glacial acetic acid). After 24 h leaf samples were transferred and stored in an ethanol solution. Subsequently, leaf tissue was dehydrated using ethanol series and embedded in paraffin. By using a rotary microtomesections of 5 µm were prepared and fixed on microscopic slides. Subsequent the samples were stained using consecutive staining and washing solutions. Afterwards pictures of the leaf cross-sections were taken using a light microscope and consecutive measurements were conducted with an open source image software. Differences found in leaf cross-sections of the two CO2 treatments were detected for the palisade parenchyma. Leaf thickness, upper and lower epidermis and spongy parenchyma remained less affected under eCO2 conditions. The observed results within grapevine leaf tissues can provide first insights to seasonal adaptation strategies of grapevines under future elevated CO2 concentrations.

DOI:

Publication date: May 31, 2022

Issue: Terclim 2022

Type: Article

Authors

Yvette Wohlfahrt1, Katja Krüger2, Susanne Tittmann1 and Manfred Stoll1

1Hochschule Geisenheim University, Department of General and Organic Viticulture, Geisenheim, Germany
2University of Applied Sciences Erfurt, Erfurt Research Centre for Horticultural Crops (FGK), Erfurt, Germany

Contact the author

Keywords

leaf morphology, Vitis vinifera, carbon dioxide, FACE

Tags

IVES Conference Series | Terclim 2022

Citation

Related articles…

The relationship between wind exposure and viticultural performance of Vitis vinifera L. cv. Merlot in a coastal vineyard (South Africa)

The South Western Cape of South Africa is exposed to strong southerly and south easterly synoptic winds during the growth period of the grapevine. The development of sea breezes in the afternoon is also a phenomenon associated with the ripening period of grapes cultivated in this coastal area. Wind is one of the environmental variables having the greatest spatial variation but the implications of regular exposure to wind for the performance of the grapevine has not yet been determined for vineyards in the South Western Cape. This study was initiated to meet this need.
The study was conducted in a hedge-trellised vineyard of Vitis vinifera L. cv Merlot with north east – south west row direction. Thirty experimental sites, each consisting of 14 vines, were identified as being exposed to wind or sheltered based on hand-held anemometer readings during the 2001/2002 season. Four stationary anemometers were strategically positioned between the thirty sites. Stomatal conductance and leaf temperature were measured with a PP systems porometer. Vegetative and yield measurements were performed during the 2002/2003 season. The t-test of equal variance was used to determine significant differences in measured parameters between exposed and sheltered grapevines.
Stomatal conductance and leaf area were significantly reduced by exposure to wind. This was associated with a significant reduction in the leaf area of primary shoots, related to shorter shoots, but a significant augmentation of secondary shoot leaf number and area. The number of bunches per vine and yield were also reduced for exposed vines. The berry potassium content was significantly increased for exposed grapevines.
This demonstrates that exposure to wind can result in significant within-vineyard, and potentially between-vineyard, variability in grapevine physiology, vegetative growth, yield and berry composition, with implications for wine style and quality.

From local classification to regional zoning-the use of a geographic information system (GIS) in Franconia / Germany. Part 1: specific GIS applications in viticulture

En vue d’une production économique de qualités des raisins optimales une connaissance des informations les plus différentes est importante. Les nouvelles technologies, telles qu’un SIG permettent de réunir les informations sur le terrain, la nature du sol, le danger d’érosion, le climat, la végétation, l’hydrographie, l’apparition de nuisible et de maladies, etc. Sur la base de cartes topographiques un SIG permet une vaste analyse, une appréciation des rapports complexes ainsi qu’une représentation cartographique. Sur la base de modélisations en trois dimensions du terrain avec le SIG, les ensembles de données saisies ainsi que leur classification au niveau local peuvent être utilisés dans la production de zonages régionaux.

PESTICIDE RESIDUES IN THE VINEYARD ENVIRONMENTS: VINE LEAVES, GRAPE BERRIES, WINES, HONEYBEES AND ASIAN HORNETS

Synthetic pesticides are widely used in viticulture to ensure steady harvest quality and quantity. Fungicides are primarily used to control grapevine diseases but insecticides and herbicides are likewise used. Pesticide residues in viticultural areas currently represent a strong societal concern, but may also affect different trophic chains in such areas. In this project we wish to analyse honeybees collected from hives placed in different vineyards, their natural predator (the invasive hornet Vespa velutina), as well as the honey, grape berries, and wines produced.

Sustainable vineyard management at the regional scale: insights from a Swiss winegrowing region

Swiss wine producers are faced to high production costs and low-priced wine imports.

Sensory significance of aroma carry-over during bottling from aromatized wine-based beverages into regular wine

In 2020 one out of  eight wine bottles were filled with a flavoured wine-based beverage.