terclim by ICS banner
IVES 9 IVES Conference Series 9 Metabolomic discrimination of grapevine water status for Chardonnay and Pinot noir

Metabolomic discrimination of grapevine water status for Chardonnay and Pinot noir

Abstract

Water status impact in viticulture has been widely explored, as it strongly affects grapevine physiology and grape chemical composition. It is considered as a key component of vitivinicultural terroir. Most of the studies concerning grapevine water status have focused on either physiological traits, or berry compounds, or traits involved in wine quality. Here, the response of grapevine to water availability during the ripening period is assessed through non-targeted metabolomics analysis of grape berries by ultra-high resolution mass spectrometry. The grapevine water status has been assessed during 2 consecutive years (2019 & 2020), through carbon isotope discrimination on juices from berries collected at maturity (21.5 brix approx.) for 2 Vitis vinifera cv. Pinot noir (PN) and Chardonnay (CH). A total of 220 grape juices were collected from 5 countries worldwide (Italy; Argentina; France; Germany; Portugal). Measured δ13C (‰) varied from -28.73 to -22.6 for PN, and from -28.79 to -21.67 for CH. These results also clearly revealed higher water stress for the 2020 vintage. The same grape juices have been analysed by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR-MS) and Liquid Chromatography coupled to Mass Spectrometry (LC-qTOF-MS), leading to the detection of up to 4500 CHONS containing elemental compositions, and thus likely tens of thousands of individual compounds, which include fatty acids, organic acids, peptides, phenolics, also with high levels of glycosylation.  Multivariate statistical analysis revealed that up to 160 elemental compositions, covering the whole range of detected masses (100 –1000 m/z), were significantly correlated to the observed gradients of water status. Examples of chemical markers, which are representative of these complex fingerprints, include various derivatives of the known abscisic acid (ABA), such as phaesic acid or abscisic acid glucose ester, which are significantly correlated with higher water stress, regardless of the variety. Cultivar-specific behaviours could also be identified from these fingerprints. Our results provide an unprecedented representation of the metabolic diversity, which is involved in the water status regulation at the grape level, and which could contribute to a better knowledge of the grapevine mitigation strategy in a climate change context.

DOI:

Publication date: May 31, 2022

Issue: Terclim 2022

Type: Article

Authors

Sébastien Nicolas1, Benjamin Bois2, Kévin Billet1, Mourad Harir3, Marianna Lucio3, Olivier Mathieu2, Anne-Lise Santoni2, Roy Urvieta4, Fernando Buscema4, Héloise Mahé5, Christine Monamy5, Sébastien Debuisson6, Julie Perry6, Fernando Alves7, Agnes Destrac8, Olivier Yobregat9, Laurent Audeguin10, Manfred Stoll11, Jean-Yves Cahurel12, Florian Haas13, Marianne Henner14, Philippe Schmitt-kopplin3 and Régis D. Gougeon1

1Univ. Bourgogne Franche-Comté, AgroSup Dijon, PAM UMR A 02.102, Institut Universitaire de la Vigne et du Vin – Jules Guyot, Dijon, France
2Biogéosciences UMR 6282 CNRS/Univ Bourgogne Franche Comté – Institut Universitaire de la Vigne et du Vin, 21000 Dijon, France
3Research Unit Analytical BioGeoChemistry, Helmholtz Zentrum München, Neuherberg, Germany
4Catena Institute of Wine, Bodega Catena Zapata, Mendoza, Argentina
5Bureau Interprofessionnel des Vins de Bourgogne, Centre Interprofessionnel Technique, Beaune, France
6CIVC, Comité interprofessionnel du vin de Champagne, Epernay, France
7Symington,  Vila Nova de Gaia, Portugal
8EGFV, Univ. Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV, Villenave d’Ornon, France
9Institut Français de la Vigne et du Vin Pôle Sud-Ouest, V’innopôle, Lisle Sur Tarn, France
10Institut Français de la Vigne et du Vin, Domaine de l’Espiguette, Le Grau du Roi, France
11Institut für Weinbau und Rebenzüchtung, Fachgebiet Weinbau, Forschungsanstalt Geisenheim, Geisenheim, Germany
12Institut Français de la Vigne et du Vin, Pôle Bourgogne – Beaujolais – Jura – Savoie, Villefranche/Saône cedex, France
13Laimburg Research Centre, Ora, Italy
14Chambre d’agriculture Alsace, Sainte-croix-en-plaine, France

Contact the author

Keywords

 climate change, water stress, mass spectrometry, untargeted metabolomics, Pinot noir, Chardonnay

Tags

IVES Conference Series | Terclim 2022

Citation

Related articles…

The surprising role of VvLYK6 in grapevine immune responses triggered by chitin oligomers

For sustainable viticulture, the substitution of chemical inputs with biocontrol products has become one of the most considered strategies. This strategy is based on elicitor-triggered immunity that requires a deep understanding of the molecular mechanisms involved in plant defense activation. Plant immune responses are triggered through the perception of conserved microbe-associated molecular patterns (MAMPs) which are recognized by pattern recognition receptors (PRRs) at the plasma membrane.

Digitalization and valorization of the genotypic and phenotypic information retained within the FEM grapevine germplasm

The maintenance and valorization of genetic diversity is an undoubtable resource for the viticulture of the future, since the climate crisis is forcing us to think of new, more resilient varieties. For this reason, the grapevine germplasm of the Fondazione Edmund Mach has been continuously expanded in the last decade to a total of 3,120 accessions, whose trueness-to-type has been verified by means of the universal set of nine microsatellites. About two thirds are V. vinifera subsp. vinifera accessions, while the rest consists of naturalized and selected hybrids, V. vinifera subsp. sylvestris, and pure species. The genetic material has also been characterized over three consecutive years for ampelographic, vine development, and biotic stress response traits to be exploited for experimental purposes.

Analysis of peptide fraction from white wines

Among nitrogen compounds included in white wines, the peptide fraction is certainly the least studied, however this fraction is quantitatively the most important (Feuillat, 1974). Existing studies concern the fraction below 1 kDa and only for white and sparkling wines (Bartolomé et al, 1997, Desportes et al 2000). In this report, we have developed methods to isolate peptides from reference white wines. Then, we have applied this methodology with bitter wine to answer a research question: is there a relation between peptides and the bitterness of white wine as for some cheese for example (Furtado, 1984)?

Isotopes to distinguish production system in Brazilian viticulture

Organic viticulture integrates practices aimed at foresting positive relationships among, vines, soil, and climate, with a focus on sustainability, social responsibility, and environmental protection. To safeguard production integrity, regulatory bodies worldwide conduct organic certifications in accordance with relevant regulations. Considering that agriculture practices influence the nitrogen, carbon and oxygen isotope composition, the study aimed to investigate the response of these isotopes in grape must cultivated by organic, biodynamic and conventional methods to distinguish between production systems.

Variabilité des critères de délimitation dans les AOC françaises

La délimitation de l’aire de production d’une appellation d’origine contrôlée française est une opération essentielle. Le décret-loi du 30 juillet 1935, qui a créé le système des appellations d’origine contrôlées