IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Hyperspectral imaging and Raman spectroscopy, nondestructive methods to assess wine grape composition

Hyperspectral imaging and Raman spectroscopy, nondestructive methods to assess wine grape composition

Abstract

Grape composition is of high interest for producing quality wines. For that, grape analyses are necessary, and they still require sample preparation, whether with classical analyses or with NIR analyses. The aim of the study was to test the ability of two nondestructive analyses, directly on grapes, hyperspectral imaging (HSI) and Raman spectroscopy to assess their composition.
For that, 7 grape varieties were analyzed for 2 vintages. Each grape was characterized by its technological ripening (levels of sugars, organic acids and pH) and its phenolic ripeness (total phenolic, total flavonoids, total anthocyanins contents, as well as extractable phenolic, extractable flavonoids, extractable anthocyanins, values obtained from a model wine maceration from skins, and color intensity). Spectra were recorded on 100 and 40 fresh berries per date and variety respectively with hyperspectral imaging and Raman. Raw data underwent different pretreatments (SNV, 1st and 2nd derivative) and PLS-R were then realized in order to provide models to assess grape composition.
The results showed that the 1st derivative data pretreatment generated better models and was then kept for all following analyses. Both methods, Raman spectroscopy and hyperspectral imaging, showed good ability to assess technological ripening parameters (sugar and acid contents) as well as phenolic content (TPI, Total Phenolics, Total Anthocyanins, Total Flavonoids and their extractable equivalents) (with globally R² > 0.81). However, it was not possible to reach the color intensity of grapes.
Even if both methods have the potential to assess wine grape quality on 11 important parameters, the quality of the models generated in our study was dependent on the quality parameter, the type of grapes (color) and the method, except for fructose, TSS and Extractable Anthocyanin contents, which were equivalent. Thus, the glucose concentration and the Total Phenolic Index (TPI) were better assessed by Raman spectroscopy, whereas Extractable Phenolics content was better estimated by HSI for both white and red grapes as well as Total  Anthocyanin content. Tartaric acid, Total Flavonoids, Color Intensity and extractable Flavonoids were better assessed by HSI for red grapes but by Raman for white grapes.
The quality of the generated models was yet dependent on the color of grapes and the parameter considered. More data would be necessary to strengthen the models but the proof of concept was successful with this study

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Maury Chantal¹, Gabrielli Mario², Ounaissi Daoud¹, Lançon-Verdier Vanessa¹, Julien Séverine¹and Le Meurlay Dominique ¹

¹USC 1422 GRAPPE, INRAE, Ecole Supérieure d’Agricultures, SFR 4207 QUASAV
²Dipartimento di Scienze e Tecnologie Alimentari per una filiera agro-alimentare Sostenibile, Università Cattolica del Sacro Cuore

Contact the author

Keywords

wine grape, hyperspectral imaging, Raman spectroscopy, phenolics, composition

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Spatiotemporal patterns of chemical attributes in Vitis vinifera L. cv. Cabernet Sauvignon vineyards in Central California

Spatial variability of vine productivity in winegrapes is important to characterise as both yield and quality are relevant for the production of different wine styles and products. The objectives were to understand how patterns of variability of Cabernet Sauvignon fruit composition changed over time and space, how these patterns could be characterised with indirect measurements, and how spatial patterns of the variation in fruit compositional attributes can aid in improving management. Prior to the 2017 vintage, 125 data vines were distributed across each of four vineyards in the Lodi American Viticultural Area (AVA) of California. Each data vine was sampled at commercial harvest in 2017, 2018, and 2019. Yield components and fruit composition were measured at harvest for each data vine, and maps of yield and fruit composition were produced for eight ‘objective measures of fruit quality’: total anthocyanins, polymeric tannins, quercetin glycosides, malic acid, yeast assimilable nitrogen, β-damascenone, C6 alcohols and aldehydes, and 3-isobutyl-2-methoxypyrazine. Patterns of variation in anthocyanins and phenolic compounds were found to be most stable over time. Given this relative stability, management decisions focused on fruit quality could be based on zonal descriptions of anthocyanins or phenolics to increase profitability in some vineyards. In each vineyard, dormant season pruning weights and soil cores were collected at each location, elevation and soil apparent electrical conductivity surveys were completed, and remotely sensed imagery was captured by fixed wing aircraft and two satellite platforms at major phenological stages. The data collected were used to develop relationships among biophysical data, soil, imagery, and fruit composition. The standardised and aggregated samples from four vineyards over three seasons were included in the estimation of ‘common variograms’ to assess how this technique could aid growers in producing geostatistically rigorous maps of fruit composition variability without cumbersome, single season sampling efforts.

The plantation frame as a measure of adaptation to climate change

The mechanization of vineyard work originally led to a reduction in planting densities due to the lack of machinery adapted to the vineyard. The current availability of specific machinery makes it possible to establish higher planting densities. In this work, three planting densities (1.40×0.80 m, 1.80×1 m and 2.20×1.20 m, corresponding to 8928, 5555 and 3787 plants/ha respectively) were studied with four varieties autochthonous of Galicia (northwestern Spain): Albariño and Treixadura (white), Sousón and Mencía (red). The vines were trained in a vertical shoot positioning system using a single Royat cordon, and pruned to spurs with two buds each. Agronomic data (yield, pruning wood weight, Ravaz index) and oenological data in must were collected. The higher planting density (1.40×0.80 m) had no significant effect on grape yield per vine in white varieties, although production per hectare was much higher due to the greater number of plants. In red varieties, this planting density resulted in a significantly lower production per vine, compensated by the greater number of plants. In addition, it significantly reduced the Brix degree in the must of the Albariño, Treixadura and Sousón varieties, and increased the total acidity in the latter two and Mencía. It also caused an increase in extractable and total anthocyanins and IPT in red grapes. The effects of high planting density on grapes are of great interest for the adaptation of varieties in the context of climate change. In the future, it could be advisable to modify the limits imposed by the appellations of origin on the planting density of these varieties in order to obtain more balanced wines.

Mesoclimate impact on Tannat in the Atlantic terroir of Uruguay

The study of climate is relevant as an element conditioning the typicity of a product, its quality and sustainability over the years. The grapevine development and growth and the final grape and wine composition are closely related to temperature, while climate components vary at mesoscale according to topography and/or proximity to large bodies of water. The objective of this work is to assess the mesoclimate of the Atlantic region of Uruguay and to determine the effect of topography and the ocean on temperature and consequently on Tannat grapevine behavior.

Different soil types and relief influence the quality of Merlot grapes in a relatively small area in the Vipava Valley (Slovenia) in relation to the vine water status

Besides location and microclimatic conditions, soil plays an important role in the quality of grapes and wine. Soil properties influence…

Rapid damage assessment and grapevine recovery after fire

There is increasing scientific consensus that climate changeis the underlying cause of the prolonged dry and hot conditions that have increased the risk of extreme fire weather in many countries around the world. In December 2019, a bushfire event occurred in the Adelaide Hills, South Australia where 25,000 hectares were burnt and in vineyards and surrounding areas various degrees of scorching and infrastructure damage occurred. The ability to coordinate and plan recovery after a fire event relies on robust and timely data. The current practice for measuring the scale and distribution of fire damage is to walk or drive the vineyard and score individual vines based on visual observation. The process is time consuming, subjective, or semi-quantitative at best. After the December 2019 fires, it took many months to access properties and estimate the area of vineyard damaged. This study compares the rapid assessment and mapping of fire damage using high-resolution satellite imagery with more traditional ground based measures. Satellite imagery tracking vineyard recovery in the season following the bushfire is being correlated to field assessments of vineyard productivity such as canopy health and development, fertility and carbohydrate storage. Canopy health in the seasons following the fires correlated to the severity of the initial fire damage. Severely damaged vines had reduced canopy growth, were infertile or had very low fertility as well as lower carbohydrate levels in buds and canes during dormancy, which reduced productivity in the seasons following the bushfire event. In contrast, vines that received minor damage were able to recover within 1-2 years. Tools that rapidly and affordably capture the extent and severity of damage over large vineyard area will allow producers, government and industry bodies to manage decisions in relation to fire recovery planning, coordination and delivery, improving the efficiency and effectiveness of their response.