IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Effect of foliar treatment of methyl jasmonate and nanoparticles doped with methyl jasmonate on Monastrell grape skin cell wall

Effect of foliar treatment of methyl jasmonate and nanoparticles doped with methyl jasmonate on Monastrell grape skin cell wall

Abstract

The use of elicitors to promote the biosynthesis of secondary metabolites in grapes has been tackled in several reports, however its study linked to nanotechnology is less developed. On the other hand, many works have studied the phenomenon of the extraction in the wine of the compounds that are present in the skin which is affected by several factors as maceration time, temperature, pH, alcoholic degree, use of enzymes. Other important factor to take into account in their extractability of these compounds is the grape cell wall composition, influenced by variety, culture practices, climate conditions, and its facility to be break down. Thus, in this work we present for the first time, the effect  of methyl jasmonate (MeJ) in conventional way and  as nanoparticles doped with MeJ (nano-MeJ) on the cell wall composition of Monastrell and also the relation with its wine phenolic content.Three foliar treatments were applied by triplicate on veraison and a week later, with 10 vines each replicate:  i) Control, ii) MeJ 10 mM  and iii) Nano-MeJ (equivalent to 1mM in MeJ) over three seasons (2019, 2020 and 2021).Cell wall material was isolated using the procedure described by Paladines-Quezada et al. [1]. Uronic acids, proteins, total phenols and cellulosic glucose were analysed following the methodology propose by Apolinar-Valiente et al. [2] .On the other hand the following parameters were evaluated in wine: colour intensity, total polyphenols index and anthocyanins.Regarding cell wall composition, the results showed a decrease in the concentration of cellulosic glucose and total phenols, an increased in proteins and specially in the uronic acids in both treatments applied.With respect to the wine results, the highest anthocyanin concentration was obtained in wines elaborated with MeJ treated grapes but this increase was not evident in wines elaborated with grapes treated with nano-MeJ. So it is possible that the interaction of the increment in proteins and uronic acids and the reduction of cellulosic glucose in the cell wall for this treatment diminished the extractability of phenolic compounds into wine. Other possibility is that the concentration applied in the form of nanoparticles was not sufficient to increase its amount of phenolic compounds in grapes and therefore in wines.Finally, all the parameters studied in grapes and wines were affected by the season studied, being evident the interaction between treatment and year for all of them except for cellulosic glucose and colour intensity. 

References

1. Paladines-Quezada, D.F.; Moreno-Olivares, J.D.; Fernández-Fernández, J.I.; Bautista-Ortín, A.B.; Gil-Muñoz, R. Influence of methyl jasmonate and benzothiadiazole on the composition of grape skin cell walls and wines. Food Chem. 2019, 277, 691–697, doi:10.1016/j.foodchem.2018.11.029.
2. Apolinar-Valiente, R.; Romero-Cascales, I.; Gómez-Plaza, E.; López-Roca, J.M.; Ros-García, J.M. Cell wall compounds of red grapes skins and their grape marcs from three different winemaking techniques. Food Chem. 2015, 187, 89–97, doi:10.1016/j.foodchem.2015.04.042.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Poster

Authors

Giménez-Bañón María José1, Paladines-Quezada Diego F.1, Moreno-Olivares Juan D.1, Parra-Torrejón Belén2, Ramírez-Rodríguez Gloria B.2, Delgado-López José M.2, Fernández-Fernández José-Ignacio1 and Gil-Muñoz Rocío1

1Instituto Murciano de Investigación y Desarrollo Agrario y Medioambiental (IMIDA)
2Departamento de Química Inorgánica, Facultad de ciencias, Universidad de Granada

Contact the author

Keywords

elicitor, nanotechnology, anthocianyn, uronic acids, sustainable agriculture

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Modulation of berry composition by different vineyard management practices

High concentration of sugars in grapes and alcohol in wines is one of the consequences of climate change on viticulture production in several wine-growing regions. In order to investigate the possibilities of adaptation of vineyard management practices aimed to reduce the accumulation of sugar during the maturation phase without reducing the accumulation of anthocyanins in grapes, a study with severe shoot trimming, shoot thinning, cluster thinning and date of harvest was conducted on Merlot variety in Istria region (Croatia), under the Mediterranean climate. Four factors which may affect grape maturation and its composition at harvest were investigated in a two-years experiment; severe shoot trimming applied at veraison when >80% of berries changed colour (in comparison to untreated control), shoot thinning (0 and 30%), cluster thinning (0 and 30%), and the date of harvest (early and standard harvest dates). Shoot thinning had no significant impact on berry composition, despite the obtained reduction in yield per vine. Lower Brix in grapes were obtained with earlier harvest date and if no cluster thinning was applied, although at the same time a reduction in the concentration of anthocyanins in berries was observed in these treatments. On the other hand, if severe shoot trimming was applied when >80% of berries changed colour, a reduction of Brix was obtained without a negative impact on berry anthocyanins concentration. We conclude that in cases when undesirably high sugar concentrations at harvest are expected, severe shoot trimming at 80% veraison may effectively be used in order to obtain moderate sugar concentration in berries together with the adequate phenolic composition.

Heatwaves and grapevine yield in the Douro region, crop model simulations

Heatwaves or extreme heat events can be particularly harmful to agriculture. Grapevines grown in the Douro winemaking region are particularly exposed to this threat, due to the specificities of the already warm and dry climatic conditions. Furthermore, climate change simulations point to an increase in the frequency of occurrence of these extreme heat events, therefore posing a major challenge to winegrowers in the Mediterranean type climates. The current study focuses on the application of the STICS crop model to assess the potential impacts of heatwaves in grapevine yields over the Douro valley winemaking region. For this purpose, STICS was applied to grapevines using high-resolution weather, soil and terrain datasets over the Douro. To assess the impact of heatwaves, the weather dataset (1989-2005) was artificially modified, generating periods with anomalously high temperatures (+5 ºC), at certain onset dates and with specific durations (from 5 to 9 days). The model was run with this modified weather dataset and results were compared to the original unmodified runs. The results show that heatwaves can have a very strong impact on grapevine yields, strongly depending on the onset dates and duration of the heatwaves. The highest negative impacts may result in a decrease in the yield by up to -35% in some regions. Despite some uncertainties inherent to the current modelling assessment, the present study highlights the negative impacts of heatwaves on viticultural yields in the Douro region, which is critical information for stakeholders within the winemaking sector for planning suitable adaptation measures.

Late season canopy management practices to reduce sugar loading and improve color profile of Cabernet-Sauvignon grapes and wines in the high irradiance and hot conditions of California Central Valley

Global warming is accelerating grape ripening, leading to unbalanced wines from fruit with high sugar content but poor aroma and colour development. Reducing the size of the photosynthetic apparatus after veraison has been shown to delay technological ripeness in cool climates, but methods have not been tested in areas with high irradiance and temperature where fruit exposure could have disastrous effects on berry composition. In this Cabernet-Sauvignon trial, we compared the application of an antitranspirant (pinolene), to severe canopy topping and above bunch zone leaf removal, all performed at mid-ripening, with an untouched control. We monitored the vines weekly by measuring stem water potential, gas exchange, fruit zone light exposure. We sampled berries to measure berry weight, total soluble solids, pH, titratable acidity, and the anthocyanin profile. At harvest, we assessed yield components, measured carbon isotope discrimination, rated sunburn on clusters, and produced experimental wines. We submitted harvest samples to metabolomic profiling through PFP-Q Exactive MS/MS and wines to sensory analysis. Application of the antitranspirant significantly reduced stomatal conductance and assimilation rate but did not affect the stem water potential. Inversely, leaf removal and topping increased water potential but did not affect leaf gas exchange. The late topping was the only treatment able to decrease sugar content (up to 2Bx), increase titratable acidity and pH, and improve anthocyanin content because of lower degradation of di-hydroxylated forms. Late leaf removal above the bunch zone increased lightning conditions in the canopy and produced the most significant damage on fruits. Yield components were not affected. This work suggests that late-season canopy management can effectively control ripening speeds and improve grapes and wines. Still, the effect on grape exposure in a critical time must be well balanced to avoid problems with the appropriate technique.

Variety and climatic effects on quality scores in the Western US winegrowing regions

Wine quality is strongly linked to climate. Quality scores are often driven by climate variation across different winegrowing regions and years, but also influenced by other aspects of terroir, including variety. While recent work has looked at the relationship between quality scores and climate across many European regions, less work has examined New World winegrowing regions. Here we used scores from three major rating systems (Wine Advocate, Wine Enthusiast and Wine Spectator) combined with daily climate and phenology data to understand what drives variation across wine quality scores in major regions of the Western US, including regions in California, Oregon and Washington. We examined effects of variety, region, and in what phenological period climate was most predictive of quality. As in other studies, we found climate, based mainly on growing degree day (GDD) models, was generally associated with quality—with higher GDD associated with higher scores—but variety and region also had strong effects. Effects of region were generally stronger than variety. Certain varieties received the highest scores in only some areas, while other varieties (e.g., Merlot) generally scored lower across regions. Across phenological stages, GDD during budbreak was often most strongly associated with quality. Our results support other studies that warmer periods generally drive high quality wines, but highlight how much region and variety drive variation in scores outside of climate.

Spatial determination of areas in the Western Balkans region favorable for organic production

In problematic conditions for production of grapes and wine caused by the COVID-19 pandemic and the resulting occurrence of wine surpluses, producers are increasingly turning to the innovative viticulture and winemaking of products that are more appealing to the market and the consumers. On the other hand, consumption of the food safety or organic products, and therefore of organic grapes and wine, is increasingly common in the world, in particular in Europe. The Regional Rural Development Standing Working Group (SWG RRD), as a regional intergovernmental organization gathers actors in the viticulture and winemaking sector from states and territories of the Western Balkans (South-East Europe) in the Expert Working Group for Wine, with the aim of improving viticulture and winemaking in this region through joint activities. In accordance with the aforementioned, the SWG RRD is working on advancing organic production of grapes and wine, and on recognition of specificities of the terroir of wine-growing areas in Western Balkans. In addition, as part of the project “Facilitation of Exchange and Advice on Wine Regulations in Western Balkan Countries” helmed by the German Federal Ministry of Food and Agriculture, in addition to harmonization of relevant legislation with EU regulations, efforts are being invested towards recognition of organic wines. Within activities and project implemented by this organization, expert analyses and scientific research of the terroir of Western Balkans were carried out, and some of the results are presented in this paper.