IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Effect of foliar treatment of methyl jasmonate and nanoparticles doped with methyl jasmonate on Monastrell grape skin cell wall

Effect of foliar treatment of methyl jasmonate and nanoparticles doped with methyl jasmonate on Monastrell grape skin cell wall

Abstract

The use of elicitors to promote the biosynthesis of secondary metabolites in grapes has been tackled in several reports, however its study linked to nanotechnology is less developed. On the other hand, many works have studied the phenomenon of the extraction in the wine of the compounds that are present in the skin which is affected by several factors as maceration time, temperature, pH, alcoholic degree, use of enzymes. Other important factor to take into account in their extractability of these compounds is the grape cell wall composition, influenced by variety, culture practices, climate conditions, and its facility to be break down. Thus, in this work we present for the first time, the effect  of methyl jasmonate (MeJ) in conventional way and  as nanoparticles doped with MeJ (nano-MeJ) on the cell wall composition of Monastrell and also the relation with its wine phenolic content.Three foliar treatments were applied by triplicate on veraison and a week later, with 10 vines each replicate:  i) Control, ii) MeJ 10 mM  and iii) Nano-MeJ (equivalent to 1mM in MeJ) over three seasons (2019, 2020 and 2021).Cell wall material was isolated using the procedure described by Paladines-Quezada et al. [1]. Uronic acids, proteins, total phenols and cellulosic glucose were analysed following the methodology propose by Apolinar-Valiente et al. [2] .On the other hand the following parameters were evaluated in wine: colour intensity, total polyphenols index and anthocyanins.Regarding cell wall composition, the results showed a decrease in the concentration of cellulosic glucose and total phenols, an increased in proteins and specially in the uronic acids in both treatments applied.With respect to the wine results, the highest anthocyanin concentration was obtained in wines elaborated with MeJ treated grapes but this increase was not evident in wines elaborated with grapes treated with nano-MeJ. So it is possible that the interaction of the increment in proteins and uronic acids and the reduction of cellulosic glucose in the cell wall for this treatment diminished the extractability of phenolic compounds into wine. Other possibility is that the concentration applied in the form of nanoparticles was not sufficient to increase its amount of phenolic compounds in grapes and therefore in wines.Finally, all the parameters studied in grapes and wines were affected by the season studied, being evident the interaction between treatment and year for all of them except for cellulosic glucose and colour intensity. 

References

1. Paladines-Quezada, D.F.; Moreno-Olivares, J.D.; Fernández-Fernández, J.I.; Bautista-Ortín, A.B.; Gil-Muñoz, R. Influence of methyl jasmonate and benzothiadiazole on the composition of grape skin cell walls and wines. Food Chem. 2019, 277, 691–697, doi:10.1016/j.foodchem.2018.11.029.
2. Apolinar-Valiente, R.; Romero-Cascales, I.; Gómez-Plaza, E.; López-Roca, J.M.; Ros-García, J.M. Cell wall compounds of red grapes skins and their grape marcs from three different winemaking techniques. Food Chem. 2015, 187, 89–97, doi:10.1016/j.foodchem.2015.04.042.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Poster

Authors

Giménez-Bañón María José1, Paladines-Quezada Diego F.1, Moreno-Olivares Juan D.1, Parra-Torrejón Belén2, Ramírez-Rodríguez Gloria B.2, Delgado-López José M.2, Fernández-Fernández José-Ignacio1 and Gil-Muñoz Rocío1

1Instituto Murciano de Investigación y Desarrollo Agrario y Medioambiental (IMIDA)
2Departamento de Química Inorgánica, Facultad de ciencias, Universidad de Granada

Contact the author

Keywords

elicitor, nanotechnology, anthocianyn, uronic acids, sustainable agriculture

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Measurement of redox potential as a new analytical winegrowing tool

Excell laboratory has initiated the development of an analytical method based on electrochemistry to evaluate the ability of wines to undergo or resist to oxidative phenomena. Electrochemistry is a powerful tool to probe reactions involving electron transfers and offers possibility of real-time measurements. In that context, the laboratory has implemented electrochemical analysis to assess oxidation state of different wine matrices but also in order to evaluate oxidative or reduced character of leaf and soil. Initially, our laboratory focused on dosage of compounds involved in responses of plant stresses and we were also interested in microbiological activity of soils. These analyses were compared with the measurement of redox potential (Eh) and pH which are two fundamental variables involved in the modulation of plant metabolism. Indeed, the variation of redox states of the plant reflects its biological activity but also its capacity to absorb nutriments. The Eh-pH conditions mainly determine metabolic processes involved in soil and leaf and our goal is to determine if this combined analytical approach will be sufficiently precise to detect biological evolutions (plant health, parasitic attack…).

Underpinning terroir with data: rethinking the zoning paradigm

Agriculture, natural resource management and the production and sale of products such as wine are increasingly data-driven activities. Thus, the use of remote and proximal crop and soil sensors to aid management decisions is becoming commonplace and ‘Agtech’ is proliferating commercially; mapping, underpinned by geographical information systems and complex methods of spatial analysis, is widely used. Likewise, the chemical and sensory analysis of wines draws on multivariate statistics; the efficient winery intake of grapes, subsequent production of wines and their delivery to markets relies on logistics; whilst the sales and marketing of wines is increasingly driven by artificial intelligence linked to the recorded purchasing behaviour of consumers. In brief, there is data everywhere!

Opinions will vary on whether these developments are a good thing. Those concerned with the ‘mystique’ of wine, or the historical aspects of terroir and its preservation, may find them confronting. In contrast, they offer an opportunity to those interested in the biophysical elements of terroir, and efforts aimed at better understanding how these impact on vineyard performance and the sensory attributes of resultant wines. At the previous Terroir Congress, we demonstrated the potential of analytical methods used at the within-vineyard scale in the development of Precision Viticulture, in contributing to a quantitative understanding of regional terroir. For this conference, we take this approach forward with examples from contrasting locations in both the northern and southern hemispheres. We show how, by focussing on the vineyards within winegrowing regions, as opposed to all of the land within those regions, we might move towards a more robust terroir zoning than one derived from a mixture of history, thematic mapping, heuristics and the whims of marketers. Aside from providing improved understanding by underpinning terroir with data, such methods should also promote improved management of the entire wine value chain.

Use of a new, miniaturized, low-cost spectral sensor to estimate and map the vineyard water status from a mobile 

Optimizing the use of water and improving irrigation strategies has become increasingly important in most winegrowing countries due to the consequences of climate change, which are leading to more frequent droughts, heat waves, or alteration of precipitation patterns. Optimized irrigation scheduling can only be based on a reliable knowledge of the vineyard water status.

In this context, this work aims at the development of a novel methodology, using a contactless, miniaturized, low-cost NIR spectral tool to monitor (on-the-go) the vineyard water status variability. On-the-go spectral measurements were acquired in the vineyard using a NIR micro spectrometer, operating in the 900–1900 nm spectral range, from a ground vehicle moving at 3 km/h. Spectral measurements were collected on the northeast side of the canopy across four different dates (July 8th, 14th, 21st and August 12th) during 2021 season in a commercial vineyard (3 ha). Grapevines of Vitis vinifera L. Graciano planted on a VSP trellis were monitored at solar noon using stem water potential (Ψs) as reference indicators of plant water status. In total, 108 measurements of Ψs were taken (27 vines per date).

Calibration and prediction models were performed using Partial Least Squares (PLS) regression. The best prediction models for grapevine water status yielded a determination coefficient of cross-validation (r2cv) of 0.67 and a root mean square error of cross-validation (RMSEcv) of 0.131 MPa. This predictive model was employed to map the spatial variability of the vineyard water status and provided useful, practical information towards the implementation of appropriate irrigation strategies. The outcomes presented in this work show the great potential of this low-cost methodology to assess the vineyard stem water potential and its spatial variability in a commercial vineyard.

A multidisciplinary approach to evaluate the effects of the training system on the performance of “Aglianico del Vulture” vineyards

Vineyards are complex agro-ecosystems with high spatial and temporal variability. An efficient training system may counteract the adverse effects of this variability. Moreover, considering the climate change issues, choosing an efficient training system that enhances water use and protects the vines from radiative thermal stress has become a priority for the farmers. A multidisciplinary approach that assesses the soil-crop-yield-wine relationships of vineyards in a distributed and holistic way could bring added knowledge on the behavior of the different training systems. This ongoing research aimed to implement a multidisciplinary approach to study the behavior of “Aglianico del Vulture” grapevines trained with two different systems: a spurred cordon (SC) and an “Alberello in parete” (AL), grown in a high-quality wine production area of Basilicata region (Italy). The approach merged several methods and scales of soil, ecophysiology, must/wine quality, and spectral data collection to assess the influence of the training system. Homogeneous zones (HZs) in both training systems were defined through a procedure based on geomorphological classification, unmanned aerial vehicles (UAV) images analysis, and a traditional soil survey supported by geophysical scanning. During the 2021 season, TDR probes monitored soil water content, while grapevine health status was assessed using eco-physiological measurements (LWP, chlorophyll content, PSII photosynthetic efficiency, LAI, and point-based field spectroscopy). These grapevine in-vivo measurements validated the spectral vegetation indexes (NDVI, RENDVI, CVI, and TVI) derived from the UAV multispectral imagery, which monitored the grapevine status in a distributed and non-invasive way. Grape yield, quality of berries, must and wine were measured to assess the effects of the training systems. The first experimental year results showed the variability of the vineyards and revealed relationships among soil parameters, crop characteristics, and vegetation indices of the SC and AL training systems. This multidisciplinary study could bring new insights into the vineyard training system’s effects on grape yield and wine quality.

Revealing the Barossa zone sub-divisions through sensory and chemical analysis of Shiraz wine

The Barossa zone is arguably one of the most well-recognised wine producing regions in Australia and internationally; known mainly for the production of its distinct Shiraz wines. However, within the broad Barossa geographical delimitation, a variation in terroir can be perceived and is expressed as sensorial and chemical profile differences between wines. This study aimed to explore the sub-division classification across the Barossa region using chemical and sensory measurements. Shiraz grapes from 4 different vintages and different vineyards across the Barossa (2018, n = 69; 2019, n = 72; 2020, n = 79; 2021, n = 64) were harvested and made using a standardised small lot winemaking procedure. The analysis involved a sensory descriptive analysis with a highly trained panel and chemical measurement including basic chemistry (e.g. pH, TA, alcohol content, total SO2), phenolic composition, volatile compounds, metals, proline, and polysaccharides. The datasets were combined and analysed through an unsupervised, clustering analysis. Firstly, each vintage was considered separately to investigate any vintage to vintage variation. The datasets were then combined and analysed as a whole. The number of sub-divisions based on the measurements were identified and characterised with their sensory and chemical profile and some consistencies were seen between the vintages. Preliminary analysis of the sensory results showed that in most vintages, two major groups could be identified characterised with one group showing a fruit-forward profile and another displaying savoury and cooked vegetables characters. The exploration of distinct profiles arising from the Barossa wine producing region will provide producers with valuable information about the regional potential of their wine assisting with tools to increase their target market and reputation. This study will also provide a robust and comprehensive basis to determine the distinctive terroir characteristics which exist within the Barossa wine producing region.