IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 The interaction between wine polyphenolic classes and poly-L-proline is impacted by oxygen

The interaction between wine polyphenolic classes and poly-L-proline is impacted by oxygen

Abstract

Oxygen plays a key role in the evolution of wine chemistry, within the non-volatile matrix. Polyphenol composition and structure, as well as the process of tannin polymerisation are directly impacted by oxidation, and this can occur during both fermentation and ageing. Polyphenols play an important role in red wine and exhibit a wide diversity in their structure and properties. They are responsible for wine colour, texture and taste (astringency, bitterness) and exhibit some health properties. The principal class of non-flavonoid polyphenolic compounds are the phenolic acids and stilbenes. Among the flavonoids, anthocyanins and tannins are the major structural classes. The aim of this study was to characterise the detailed response of wine polyphenolic structure and composition to an oxygen treatment applied during fermentation. A specific focus was to determine the interaction of discrete polyphenolic classes with poly-L-proline (PLP). A control Shiraz wine was prepared under reductive conditions during fermentation, in triplicate. To the same grape source, an aeration treatment was initiated on day 3 following a 1.8 °Bé decrease for 48 h at 5 L/min, also in triplicate.  After a 12-month ageing period, wines were fractionated where: F1 = Phenolic acids, F2 = flavan-3-ol monomers, F3 = flavan-3-ol oligomers, F4 = anthocyanins, pyranoanthocyanins; and F5 = polymeric proanthocyanidins, pigmented proanthocyanins and other derived complexes. The composition of fractions F1 to F4 was verified by LC-MS, and F5 was characterised by a combination of analytical techniques specific to proanthocyanidins. The interaction between the polyphenol fractions and PLP was measured by isothermal titration calorimetry (ITC). A strong binding interaction was observed between F1, the phenolic acids, and PLP by ITC, and was not affected by the oxygen treatment. In fact, a strong hydrophobic interaction and hydrogen bonding was implicated in the interaction. It was found that for fractions F2 and F3, no binding events with PLP were observed by ITC, irrespective of the oxygen level applied. Stronger binding events with PLP were observed for the F4 and F5 polyphenolic fractions, but interestingly, only in those prepared from wines which had oxygen treatment. Moreover, hydrophobic interaction and hydrogen bonding was detected just for the oxygen treatment for F4 and F5. Contrary to expectation, no binding with PLP could be detected for F4 and F5 from the control wine. Further investigation of the properties of the fractions was conducted to account for the differences observed, including their composition, hydrophobicity and aggregation. This presentation will provide new insights into the potential role of discrete polyphenolic classes in driving in-mouth sensory properties, like astringency, which might be elicited following binding with proline-rich salivary proteins.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Jouin Alicia1, Falconer Robert J.2, Waterlot Aude3, Day Martin1, Schmidt Simon1 and Bindon Keren1

1The Australian Wine Research Institute, PO Box 197, Glen Osmond, South Australia, 5064, Australia 
2Department of Chemical Engineering and Advanced Materials, University of Adelaide, Adelaide, SA, 5005, Australia
3Department of Food Science and Human Nutrition, Courtesy Faculty, Horticulture, Iowa State University, 2567 Food Sciences Building, 536 Farm House Lane, Ames, IA 50011, USA

Contact the author

Keywords

Tannins, Anthocyanins, Oxygen, Isothermal Titration Calorimetry, Astringency

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Long-term drought resilience of traditional red grapevine varieties from a semi-arid region

In recent decades, the scarcity of water resources in agriculture in certain areas has been aggravated by climate change, which has caused an increase in temperatures, changes in rainfall patterns, as well as an increase in the frequency of extreme phenomena such as droughts and heat waves. Although the vine is considered a drought-tolerant specie, it has to satisfy important water requirements to complete its cycle, which coincides with the hottest and driest months. Achieving sustainable viticulture in this scenario requires high levels of efficiency in the use of water, a scarce resource whose use is expected to be severely restricted in the near future. In this regard, the use of drought-tolerant varieties that are able to maintain grape yield and quality could be an effective strategy to face this change. During three consecutive seasons (2018-2020) the behavior in rainfed regime of 13 traditional red grapevine varieties of the Spain central region was studied. These varieties were cultivated in a collection at Centro de Investigación de la Vid y el Vino de Castilla-La Mancha (IVICAM-IRIAF) located in Tomelloso (Castilla-La Mancha, Spain). Yield components (yield, mean bunch and berry weight, pruning weight), physicochemical parameters of the musts (brix degree, total acidity, pH) and some physiological parameters related with water stress during ripening period (δ13C, δ18O) were analysed. The application of different statistical techniques to the results showed the existence of significant differences between varieties in their response to stressful conditions. A few varieties highlighted for their high ability to adapt to drought, being able to maintain high yields due to their efficiency in the use of water. In addition, it was possible quantify to what extent climate can be a determinant in the δ18O of musts under severe water stress conditions.

Extreme canopy management for vineyard adaptation to climate change: is it a good idea?

Climate change constitutes an enormous challenge for humankind and for all human activities, viticulture not being an exception. Long-term strategic changes are probably needed the most, but growers also need to deal with short-term changes: summers that are getting progressively warmer, earlier harvest dates and higher pH in musts and wines. In the last 10-15 years, a relevant corpus of research is being developed worldwide in order to evaluate to which extent extreme canopy management operations, aimed at reducing leaf area and, thus, limiting the source to sink ratio, could be useful to delay ripening. Although extreme canopy management can result in relevant delays in harvest dates, longer term studies, as well as detailed analysis of their implications on carbohydrate reserves, bud fertility and future yield are desirable before these practices can be recommended.

Impact of climate change on the viticultural climate of the Protected Designation of Origin “Jumilla” (SE Spain)

Protected Designation of Origin “Jumilla” (PDO Jumilla) is located in the Spanish provinces of Albacete and Murcia, in the South-eastern part of the Iberian Peninsula, where most of the models predict a severe impact of climate change in next decades. PDO Jumilla covers an area of 247,054 hectares, of which more than 22,000 hectares

Influence of climatic conditions on grape composition of Tempranillo in La Mancha DO (Spain)

The aim of this work was to analyze the variability in grape composition of the Tempranillo cultivar related to climatic conditions, in La Mancha Designation of Origin. Grape composition (sugar content, total acidity, pH, malic acid, and total and extractable anthocyanins) recorded during ripening, were analysed for the period 2000-2019. The weather conditions at daily time scale, recorded during the same period, were also evaluated. The relationships between grape parameters with climatic variables related to temperature and to water deficits, referring different periods between phenological events along the growing cycle, were evaluated using regression analysis. High variability in grape composition was observed in the period analysed. Total acidity varied between 3.7 and 7.3 gL-1 while malic acid varied between 1.2 and 4 gL-1. The extractable anthocyanins ranged between 526 and 972 mgL-1, and total anthocyanins ranged between 922 and 1388 mgL-1, being the lowest values recorded in the hottest year (2017). Total acidity decreased 0.77 gL-1 for an increase of 100 GDD, while malic acid decrease in 0.42 gL-1 for the same GDD increase, being the period between veraison and harvest the one that seemed to have higher influence on acidity. In addition, it was confirmed that increasing water deficits decreased acidity. Total and extractable anthocyanins increased in about 210 and 105 mgL-1, respectively, with an increase of 100 GDD from veraison to harvest, and the increase in water deficits favour the increase of anthocyanins, both total and extractable anthocyanins. Total and extractable anthocyanins concentration increased in 35 and 22 mgL-1 per an increase of 10 mm in the water deficit. These results can be of interest to understand the potential changes that grapes composition may suffer under future warmer climates.

How does aromatic composition of red wines, resulting from varieties adapted to climate change, modulate fruity aroma?

One of the major issues for the wine sector is the impact of climate change linked to the increasing temperatures which affects physicochemical parameters of the grape varieties planted in Bordeaux vineyard and consequently, the quality of wine. In some varietals, the attenuation of their fresh fruity character is accompanied by the accentuation of dried-fruit notes [1]. As a new adaptive strategy on climate change, some winegrowers have initiated changes in the Bordeaux blend of vine varieties [2]. This study intends to explore the fruitiness in wines produced from grape varieties adapted to the future climate of Bordeaux. 10 commercial single–varietal wines from 2018 vintage made from the main grape varieties in the Bordeaux region (Cabernet franc, Cabernet-Sauvignon and Merlot) as well as from indigenous grape varieties from the Mediterranean basin, such as Cyprus (Yiannoudin), France (Syrah), Greece (Agiorgitiko and Xinomavro), Portugal (Touriga Nacional) and Spain (Garnacha and Tempranillo), were selected among 19 samples using sensory descriptive analyses. Both sensory and instrumental analyses were coupled, to investigate their fruity aroma expression. For sensory analysis, samples were prepared from wine, using a semi preparative HPLC method which preserves wine aroma and isolates fruity characteristics in 25 specific fractions [3,4]. Fractions of interest with intense fruity aromas were sensorially selected for each wine by a trained panel and mixed with ethanol and microfiltered water to obtain fruity aromatic reconstitutions (FAR) [5]. A free sorting task was applied to categorize FAR according to their similarities or dissimilarities, and different clusters were highlighted. Instrumental analysis of the different FAR and wines demonstrated variations in their molecular composition. Results obtained from sensory and gas chromatography analysis enrich the knowledge of the fruity expression of red wines from “new” grape varieties opening up new perspectives in wine technology, including blending, thus providing new tools for producers.