IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 The interaction between wine polyphenolic classes and poly-L-proline is impacted by oxygen

The interaction between wine polyphenolic classes and poly-L-proline is impacted by oxygen

Abstract

Oxygen plays a key role in the evolution of wine chemistry, within the non-volatile matrix. Polyphenol composition and structure, as well as the process of tannin polymerisation are directly impacted by oxidation, and this can occur during both fermentation and ageing. Polyphenols play an important role in red wine and exhibit a wide diversity in their structure and properties. They are responsible for wine colour, texture and taste (astringency, bitterness) and exhibit some health properties. The principal class of non-flavonoid polyphenolic compounds are the phenolic acids and stilbenes. Among the flavonoids, anthocyanins and tannins are the major structural classes. The aim of this study was to characterise the detailed response of wine polyphenolic structure and composition to an oxygen treatment applied during fermentation. A specific focus was to determine the interaction of discrete polyphenolic classes with poly-L-proline (PLP). A control Shiraz wine was prepared under reductive conditions during fermentation, in triplicate. To the same grape source, an aeration treatment was initiated on day 3 following a 1.8 °Bé decrease for 48 h at 5 L/min, also in triplicate.  After a 12-month ageing period, wines were fractionated where: F1 = Phenolic acids, F2 = flavan-3-ol monomers, F3 = flavan-3-ol oligomers, F4 = anthocyanins, pyranoanthocyanins; and F5 = polymeric proanthocyanidins, pigmented proanthocyanins and other derived complexes. The composition of fractions F1 to F4 was verified by LC-MS, and F5 was characterised by a combination of analytical techniques specific to proanthocyanidins. The interaction between the polyphenol fractions and PLP was measured by isothermal titration calorimetry (ITC). A strong binding interaction was observed between F1, the phenolic acids, and PLP by ITC, and was not affected by the oxygen treatment. In fact, a strong hydrophobic interaction and hydrogen bonding was implicated in the interaction. It was found that for fractions F2 and F3, no binding events with PLP were observed by ITC, irrespective of the oxygen level applied. Stronger binding events with PLP were observed for the F4 and F5 polyphenolic fractions, but interestingly, only in those prepared from wines which had oxygen treatment. Moreover, hydrophobic interaction and hydrogen bonding was detected just for the oxygen treatment for F4 and F5. Contrary to expectation, no binding with PLP could be detected for F4 and F5 from the control wine. Further investigation of the properties of the fractions was conducted to account for the differences observed, including their composition, hydrophobicity and aggregation. This presentation will provide new insights into the potential role of discrete polyphenolic classes in driving in-mouth sensory properties, like astringency, which might be elicited following binding with proline-rich salivary proteins.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Jouin Alicia1, Falconer Robert J.2, Waterlot Aude3, Day Martin1, Schmidt Simon1 and Bindon Keren1

1The Australian Wine Research Institute, PO Box 197, Glen Osmond, South Australia, 5064, Australia 
2Department of Chemical Engineering and Advanced Materials, University of Adelaide, Adelaide, SA, 5005, Australia
3Department of Food Science and Human Nutrition, Courtesy Faculty, Horticulture, Iowa State University, 2567 Food Sciences Building, 536 Farm House Lane, Ames, IA 50011, USA

Contact the author

Keywords

Tannins, Anthocyanins, Oxygen, Isothermal Titration Calorimetry, Astringency

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Effects of graft quality on growth and grapevine-water relations

Climate change is challenging viticulture worldwide compromising its sustainability due to warmer temperatures and the increased frequency of extreme events. Grafting Vitis vinifera L.

The concept of terroir: what place for microbiota?

Microbes play key roles on crop nutrient availability via biogeochemical cycles, rhizosphere interactions with roots as well as on plant growth and health. Recent advances in technologies, such as High Throughput Sequencing Techniques, allowed to gain deeper insight on the structure of bacterial and fungal communities associated with soil, rhizosphere and plant phyllosphere. Over the past 10 years, numerous scientific studies have been carried out on the microbial component of the vineyard. Whether the soil or grape compartments have been taken into account, many studies agree on the evidence of regional delineations of microbial communities, that may contribute to regional wine characteristics and typicity. Some authors proposed the term “microbial terroir” including “yeast terroir” for grapes to describe the connection between microbial biogeography and regional wine characteristics. Many factors are involved in terroir including climate, soil, cultivar and human practices as well as their interactions. Studies considering “microbial terroir” greatly contributed to improve our knowledge on factors that shape the vineyard microbial structure and diversity. However, the potential impact of “microbial terroir” on wine composition has yet not received strong scientific evidence and many questions remain to be addressed, related to the functional characterization of the microbial community and its impact on plant physiology and grape composition, the origins and interannual stability of vineyard microbiota, as well as their impact on wine sensorial attributes. The presentation will give an overview on the role of microbiota as a terroir component and will highlight future perspectives and challenges on this key subject for the wine industry.

Rootstock regulation of scion phenotypes: the relationship between rootstock parentage and petiole mineral concentration

Grapevine is grown as a graft since the end of the 19th century. Rootstocks not only provide tolerance to Phylloxera but also ensure the supply of water and mineral nutrients to the scion. Rootstocks are an important mean of adaptation to environmental conditions, because the scion controls the typical features of the grapes and wine. However, among the large diversity of rootstocks worldwide, few of them are commercially used in the vineyard. The aim of this study was to investigate the extent to which rootstocks modify the mineral composition of the petioles of the scion. Vitis vinifera cvs. Cabernet-Sauvignon, Pinot noir, Syrah and Ugni blanc were grafted onto 55 different rootstock genotypes and planted in a vineyard as three replicates of 5 vines. Petioles were collected in the cluster zone with 6 replicates per combination. Petiolar concentrations of 13 mineral elements (N, P, K, S, Mg, Ca, Na, B, Zn, Mn, Fe, Cu, Al) at veraison were determined. Scion, rootstock and the interaction explained the same proportion of the phenotypic variance for most mineral elements. Rootstock genotype showed a significant influence on the petiole mineral element composition. Rootstock effect explained from 7 % for Cu to 25 % for S of the variance. The difference of rootstock conferred mineral status is discussed in relation to vigor and fertility. Rootstocks were also genotyped with 23 microsatellite markers. Data were analysed according to genetic groups in order to determine whether the petiole mineral composition could be related to the genetic parentage of the rootstock. Thanks to a highly powerful design, it is the first time that such a large panel of rootstocks grafted with 4 scions has been studied. These results give the opportunity to better characterize the rootstocks and to enlarge the diversity used in the vineyard.

Impact of yeast derivatives to increase the phenolic maturity and aroma intensity of wine

Using viticultural and enological techniques to increase aromatics in white wine is a prized yet challenging technique for commercial wine producers. Equally difficult are challenges encountered in hastening phenolic maturity and thereby increasing color intensity in red wines. The ability to alter organoleptic and visual properties of wines plays a decisive role in vintages in which grapes are not able to reach full maturity, which is seen increasingly more often as a result of climate change. A new, yeast-based product on the viticultural market may give the opportunity to increase sensory properties of finished wines. Manufacturer packaging claims these yeast derivatives intensify wine aromas of white grape varieties, as well as improve phenolic ripeness of red varieties, but the effects of this application have been little researched until now. The current study applied the yeast derivative, according to the manufacture’s instructions, to the leaves of both neutral and aromatic white wine varieties, as well as on structured red wine varieties. Chemical parameters and volatile aromatics were analyzed in grape musts and finished wines, and all wines were subjected to sensory analysis by a tasting panel. Collective results of all analyses showed that the application of the yeast derivative in the vineyard showed no effect across all varieties examined, and did not intensify white wine aromatics, nor improve phenolic ripeness and color intensity in red wine.

Mesoclimate impact on Tannat in the Atlantic terroir of Uruguay

The study of climate is relevant as an element conditioning the typicity of a product, its quality and sustainability over the years. The grapevine development and growth and the final grape and wine composition are closely related to temperature, while climate components vary at mesoscale according to topography and/or proximity to large bodies of water. The objective of this work is to assess the mesoclimate of the Atlantic region of Uruguay and to determine the effect of topography and the ocean on temperature and consequently on Tannat grapevine behavior.