IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 The antioxidant properties of wine lees extracts in model wine

The antioxidant properties of wine lees extracts in model wine

Abstract

While the ethanol and tartaric acid contained in wine lees are typically recovered by distilleries, the remaining solid fraction (yeast biomass) is usually disposed of, thus negatively affecting the overall sustainability of the wine industry. Previously we demonstrated that the wine lees’ solid fraction could be submitted to a food-grade physical extraction method (autoclave, 20 min, 121°C) to yield yeast polysaccharides with proven foaming, emulsifying and wine stabilizing properties [1,2]. In this study, the autoclave extraction procedure was applied directly on lees from red winemaking. As a result, two extracts were obtained: the Total extract, namely the whole lees after autoclave containing the soluble and insoluble fractions; the Supernatant, containing only the soluble compounds released during extraction. The composition of the extracts in terms of protein, polysaccharides, glutathione, total thiols, and total polyphenol content, was determined by spectrophotometric and chromatographic analytical methods. Subsequently, the extract’s oxidative behavior was tested by dissolving them (0.5 g/L) in model wine (20% EtOH, 5 g/L tartaric acid, 5 mg/L Fe, 0.5 mg/L Cu) containing 30 mg/L free SO2 and 0.5g/L catechin. The O2 and SO2 consumption, color development (as a function of catechin degradation), and linear sweep voltammetry (LSV) behavior were investigated. The effect of the wine lees’ extracts was benchmarked against analogs extracts obtained from a lab-grown culture of the same yeast strain present in the wine lees. Samples prepared with the wine lees’ extracts showed a higher O2 and SO2 consumption rates compared to those prepared with the lab-grown yeast extracts. All extracts protected the catechin from oxidation, with the best protective action achieved by the Total wine lees extract. This extract, along with its analog from the lab-grown yeast culture, showed the greatest resistance to anodic oxidation according to LSV. The protective action on catechin displayed by all the extracts was not fully explainable by their content in antioxidant compounds as glutathione, thiols, and wine polyphenols. Interestingly, the fact that the best results were obtained using the Total extracts in which both the soluble (released polysaccharides) and insoluble (yeast cell walls) fractions were present, allowed to hypothesize that other compounds are involved in limiting the catechin oxidation. In this scenario, the candidates are the yeast membrane sterols as they possess an oxygen-consuming action, and yeast cell wall polysaccharides as they could bind to catechin thus making it unavailable for oxidation. To conclude, wine lees can be considered a novel source of yeast extract with potential oenological application also against quality-affecting oxidative reactions. If adopted on a large scale,  this wine lees valorization strategy would result in an improvement of the overall sustainability of the wine industry.

References

[1] De Iseppi, A., Marangon, M., Vincenzi, S., Lomolino, G., Curioni, A., & Divol, B. (2021). A novel approach for the valorization of wine lees as a source of compounds able to modify wine properties. LWT, 136, 110274.
[2] De Iseppi, A., Marangon, M., Lomolino, G., Crapisi, A., & Curioni, A. (2021). Red and white wine lees as a novel source of emulsifiers and foaming agents. LWT, 152, 112273.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

De IseppiAlberto1, Curioni Andrea1,2, Marangon Matteo1,2, Invincibile Diletta3, Slaghenaufi Davide3 and Ugliano Maurizio3

1Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Viale dell’Università, 16, 35020 Legnaro, Padua, Italy
2Centre for Research in Viticulture and Enology (CIRVE), University of Padova, Conegliano, Italy
3Department of Biotechnology, University of Verona, San Pietro in Cariano, Italy

Contact the author

Keywords

wine lees, wine oxidation, voltammetry, wine color, by-product valorization

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

From a local to an international scale: sensory benchmarking of PDO wines. Quincy and Reuilly PDO wines (Sauvignon blanc) as a case study (France)

In a collective marketing strategy, the Protected Designation of Origin (PDO) can be used as a quality indicator. To highlight terroir specificities, it is useful to know how the wines are positioned on the local, national or international market from a sensory point of view. This is especially true for a comparison of varietal wines (e.g. Sauvignon blanc). We focus on the case of two closed Loire Valley PDO (France): Quincy and Reuilly. Three distinct tastings were organized. Firstly, at the local level comparing the 2 PDO (11 and 9 wines, 17 professional assessors); secondly at a regional level adding 3 closed PDO: Menetou-Salon, Sancerre and Pouilly-Fumé (3 wines per PDO, 16 assessors) and thirdly at an international level comparing these 5 PDO with Sauvignon Blanc wines coming from South Africa, New Zealand and Chile (1 to 3 wines per PDO, 19 assessors). All the wines were from the 2019 vintage and were considered to have a traditional elaboration process without contact with oak. A sensory descriptive analysis was performed using an aroma wheel allowing to combine a Check-All-That-Apply methodology, often used in sensory benchmarking, with a hierarchical structuration of the attributes. The aim is to facilitate data acquisition in a professional context without common training, to consider the hierarchical relationships among the attributes during the data analysis and to be able to characterize wines with a large range of sensorial variability. We use univariate, multivariate and clustering analyses. Similarities and differences between Quincy and Reuilly PDO wines and other Sauvignon blanc wines were identified. Specific attributes can distinguish the two PDO and different proximities exist with other local PDO, while clear differences were observed compared to international wines. Our study contributes to propose and discuss a method to do a wine sensory benchmarking highlighting sensory specificities linked to origin.

Inhibition of Oenococcus oeni during alcoholic fermentation by a selected Lactiplantibacillus plantarum strain

The use of selected cultures of the species Lactiplantibacillus plantarum in Oenology has grown in prominence in recent years. While initial applications of this species centred very much around malolactic fermentation (MLF), there is strong evidence to show that certain strains can be harnessed for their bio-protective effects. Unwanted spontaneous MLF during alcoholic fermentation (AF), driven by rogue Oenococcus oeni, is a winemaking deviation that is very difficult to manage when it occurs. This work set out to determine the efficacy of one particular strain of Lactiplantibacillus plantarum(Viniflora® NoVA™ Protect), against this problem in Cabernet Sauvignon must. The work was carried out at commercial scale and in a winery environment and compared the bio-protective culture with the more traditional approach of reducing must pH by the addition of tartaric acid. The combination of both was also investigated. The concentration of both Oenococcus oeni and Lactiplantibacillus plantarum was determined using qPCR. The adventitious Oenococcus oeni showed the most growth during AF in the control wine, whereas in the wines treated with Lactiplantibacillus plantarum a bacteriostatic effect against this species was observed. This effect was comparable to the wines treated with tartaric acid. This has particular commercial relevance for controlling the flora in musts with high pH, or when the addition of tartaric acid is either not permitted or is prohibitive for other reasons.

Adaptation to soil and climate through the choice of plant material

Choosing the rootstock, the scion variety and the training system best suited to the local soil and climate are the key elements for an economically sustainable production of wine. The choice of the rootstock/scion variety best adapted to the characteristics of the soil is essential but, by changing climatic conditions, ongoing climate change disrupts the fine-tuned local equilibrium. Higher temperatures induce shifts in developmental stages, with on the one hand increasing fears of spring frost damages and, on the other hand, ripening during the warmest periods in summer. Expected higher water demand and longer and more frequent drought events are also major concerns. The genetic control of the phenotypes, by genomic information but also by the epigenetic control of gene expression, offers a lot of opportunities for adapting the plant material to the future. For complex traits, genomic selection is also a promising method for predicting phenotypes. However, ecophysiological modelling is necessary to better anticipate the phenotypes in unexplored climatic conditions Genetic approaches applied on parameters of ecophysiological models rather than raw observed data are more than ever the basis for finding, or building, the ideal varieties of the future.

Local adaptation tools to ensure the viticultural sustainability in a changing climate

[lwp_divi_breadcrumbs home_text="IVES" use_before_icon="on" before_icon="||divi||400" module_id="publication-ariane" _builder_version="4.19.4" _module_preset="default" module_text_align="center" module_font_size="16px" text_orientation="center"...

Impact of climate variability and change on grape yield in Italy

Viticulture is entangled with weather and climate. Therefore, areas currently suitable for grape production can be challenged by climate change. Winegrowers in Italy already experiences the effect of climate change, especially in the form of warmer growing season, more frequent drought periods, and increased frequency of weather extremes.
The aim of this study is to investigate the impact of climate variability and change on grape yield in Italy to provide winegrowers the information needed to make their business more sustainable and resilient to climate change. We computed a specific range of bioclimatic indices, selected by the International Organisation of Vine and Wine (OIV), and correlated them to grape yield data. We have worked in collaboration with some wine consortiums in northern and central Italy, which provided grape yield data for our analysis.
Using climate variables from the E-OBS dataset we investigate how the bioclimatic indices changed in the past, and the impact of this change on grape productivity in the study areas. The climate impact on productivity is also investigated by using high-resolution convection-permitting models (CPMs – 2.2 horizontal resolution), with the purpose of estimating productivity in future emission scenarios. The CPMs are likely the best available option for this kind of impact studies since they allow a better representation of small-scale processes and features, explicitly resolve deep convection, and show an improved representation of extremes. In our study, we also compare CPMs with regional climate models (RCMs – 12 km horizontal resolution) to assess the added value of high-resolution models for impact studies. Further development of our study will lead to assessing the future suitability for vine cultivation and could lead to the construction of a statistical model for future projection of grape yield.