IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Deciphering the color of rosé wines using polyphenol targeted metabolomics

Deciphering the color of rosé wines using polyphenol targeted metabolomics

Abstract

The color of rosés wines is extremely diverse  and a key element in their marketing. It is  due to the presence of red anthocyanins extracted from grape skins and pigments formed from them and other wine constituents during wine-making. To explore the link between composition and color, 268 commercial rosé wines were analyzed by ultra-high performance liquid chromatography coupled to triple quadrupole mass spectrometry analysis in the MRM (multiple reaction monitoring) mode [1] and their color characterized using spectrophotometry. The concentration of 125 phenolic compounds was thus determined and related to color parameters using chemometrics [2]. Color intensity is primarily determined by the extent of polyphenol extraction from the grapes. However, different compositions characterize the different color styles. Dark rosé wines contain high concentrations of anthocyanins and flavanols and their color, like that of red wines, is attributable to these molecules and their reaction products. In contrast, major phenolic compounds in light rosé wines are hydroxycinnamic acids and their salmon shade is mostly due to phenylpyranoanthocyanins and carboxypyranoanthocyanin pigments, resulting from reactions of anthocyanins, respectively with these phenolic acids and with pyruvic acid, a yeast metabolite. Redness of intermediate color wines is associated to anthocyanins and carboxypyranoanthocyanins while yellowness seems related to oxidation.The same approach was applied to monitor color and composition changes during fermentation of six rosé musts made from Grenache, Cinsault and Syrah grapes. Hydroxycinnamic acids were the major phenolic compounds in Grenache and Cinsault musts while the Syrah musts showed higher concentrations of anthocyanins and flavanols, indicating that polyphenol extraction is not only related to maceration conditions but also depends on varietal characteristics. These differences resulted in different proportions of derived pigments as observed on the rosé wine collection [2]. Comparison of the spectrophotometric and MRM data indicated that the majority of phenolic compounds in the Cinsault musts were not among the compounds targeted by MRM. Size exclusion chromatography (SEC) analysis of the musts showed different profiles for the three varieties, Cinsault musts containing large proportions of oligomeric compounds likely derived from hydroxycinnamates. These larger molecular weight compounds were no longer detected after fermentation and were partly recovered from the yeast lees. Comparison of the SEC profiles obtained at different wavelengths also suggest that pigments of Cinsault and Grenache are hydroxycinnamic acid derivatives, likely resulting from enzymatic oxidation. Non targeted metabolomics approaches provided further information on these pigments.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Cheynier, Véronique1, Leborgne Cécile2, Ducasse Marie-Agnès3, Meudec Emmanuelle1, Verbaere Arnaud1, Sommerer Nicolas1, Boulet Jean-Claude1, Masson Gilles2 and Mouret Jean-Roch11

SPO, INRAE, Univ Montpellier, Institut Agro, INRAE, PROBE research infrastructure, PFP Polyphenol Analytical Facility
2 SPO, INRAE, Univ Montpellier, Institut Agro, Montpellier, France; Institut Français de la Vigne et du Vin, Centre du rosé, Vidauban, France
3 Institut Français de la Vigne et du Vin, UMT OENOTYPAGE, Domaine de Pech Rouge, Gruissan, France

Contact the author

Keywords

UHPLC-MS/MS, polyphenols, rosé wines, color, chemometrics

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Mapping and tracking canopy size with VitiCanopy

Understanding vineyard variability to target management strategies, apply inputs efficiently and deliver consistent grape quality to the winery is essential. However, despite inherent vineyard variability, the majority are managed as if they are uniform. VitiCanopy is a simple, grower-friendly tool for precision/digital viticulture that allows users to collect and interpret objective spatial information about vineyard performance. After four years of field and market research, an upgraded VitiCanopy has been created to achieve a more streamlined, technology-assisted vine monitoring tool that provides users with a set of superior new features, which could significantly improve the way users monitor their grapevines. These new features include:
• New user interface
• User authentication
• Batch analysis of multiple images
• Ease the learning curve through enhanced help features
• Reporting via the creation of colour maps that will allow users to assess the spatial differences in canopies within a vineyard.
Use-case examples are presented to demonstrate the quantification and mapping of vineyard variability through objective canopy measurements, ground-truthing of remotely sensed measurements, monitoring of crop conditions, implementation of disease and water management decisions as well as creating a history of each site to forecast quality. This intelligent tool allows users to manage grapevines and make informed management choices to achieve the desired production targets and remain profitable.

Amino nitrogen content in grapes: the impact of crop limitation

As an essential element for grapevine development and yield, nitrogen is also involved in the winemaking process and largely affects wine composition. Grape must amino nitrogen deficiency affects the alcoholic fermentation kinetics and alters the development of wine aroma precursors. It is therefore essential to control and optimize nitrogen use efficiency by the plant to guarantee suitable grape nitrogen composition at harvest. Understanding the impact of environmental conditions and cultural practices on the plant nitrogen metabolism would allow us to better orientate our technical choices with the objective of quality and sustainability (less inputs, higher efficiency). This trial focuses on the impact of crop limitation – that is a common practice in European viticulture – on nitrogen distribution in the plant and particularly on grape nitrogen composition. A wide gradient of crop load was set up in a homogeneous plot of Chasselas (Vitis vinifera) in the experimental vineyard of Agroscope, Switzerland. Dry weight and nitrogen dynamics were monitored in the roots, trunk, canopy and grapes, during two consecutive years, using a 15N-labeling method. Grape amino nitrogen content was assessed in both years, at veraison and at harvest. The close relationship between fruits and roots in the maintenance of plant nitrogen balance was highlighted. Interestingly, grape nitrogen concentration remained unchanged regardless of crop load to the detriment of the growth and nitrogen content of the roots. Meanwhile, the size and the nitrogen concentration of the canopy were not affected. Leaf gas exchange rates were reduced in response to lower yield conditions, reducing carbon and nitrogen assimilation and increasing intrinsic water use efficiency. The must amino nitrogen profiles could be discriminated as a function of crop load. These findings demonstrate the impact of plant balance on grape nitrogen composition and contribute to the improvement of predictive models and sustainable cultural practices in perennial crops.

Variations of soil attributes in vineyards influence their reflectance spectra

Knowledge on the reflectance spectrum of soil is potentially useful since it carries information on soil chemical composition that can be used to the planning of agricultural practices. If compared with analytical methods such as conventional chemical analysis, reflectance measurement provides non-destructive, economic, near real-time data. This paper reports results from reflectance measurements performed by spectroradiometry on soils from two vineyards in south Brazil. The vineyards are close to each other, are on different geological formations, but were subjected to the same management. The objective was to detect spectral differences between the two areas, correlating these differences to variations in their chemical composition, to assess the technique’s potential to predict soil attributes from reflectance data.To that end, soil samples were collected from ten selected vine parcels. Chemical analysis yield data on concentration of twenty-one soil attributes, and spectroradiometry was performed on samples. Chemical differences significant to a 95% confidence level between the two studied areas were found for six soil attributes, and the average reflectance spectra were separated by this same level along most of the observed spectral domain. Correlations between soil reflectance and concentrations of soil attributes were looked for, and for ten soil traits it was possible to define wavelength domains were reflectance and concentrations are correlated to confidence levels from 95% to 99%. Partial Least Squares Regression (PLSR) analyses were performed comparing measured and predicted concentrations, and for fifteen out of 21 soil traits we found Pearson correlation coefficients r > 0.8. These preliminary results, which have to be validated, suggest that variations of concentration in the investigated soil attributes induce differences in reflectance that can be detected by spectroradiometry. Applications of these observations include the assessment of the chemical content of soils by spectroradiometry as a fast, low-cost alternative to chemical analytical methods.

The plantation frame as a measure of adaptation to climate change

The mechanization of vineyard work originally led to a reduction in planting densities due to the lack of machinery adapted to the vineyard. The current availability of specific machinery makes it possible to establish higher planting densities. In this work, three planting densities (1.40×0.80 m, 1.80×1 m and 2.20×1.20 m, corresponding to 8928, 5555 and 3787 plants/ha respectively) were studied with four varieties autochthonous of Galicia (northwestern Spain): Albariño and Treixadura (white), Sousón and Mencía (red). The vines were trained in a vertical shoot positioning system using a single Royat cordon, and pruned to spurs with two buds each. Agronomic data (yield, pruning wood weight, Ravaz index) and oenological data in must were collected. The higher planting density (1.40×0.80 m) had no significant effect on grape yield per vine in white varieties, although production per hectare was much higher due to the greater number of plants. In red varieties, this planting density resulted in a significantly lower production per vine, compensated by the greater number of plants. In addition, it significantly reduced the Brix degree in the must of the Albariño, Treixadura and Sousón varieties, and increased the total acidity in the latter two and Mencía. It also caused an increase in extractable and total anthocyanins and IPT in red grapes. The effects of high planting density on grapes are of great interest for the adaptation of varieties in the context of climate change. In the future, it could be advisable to modify the limits imposed by the appellations of origin on the planting density of these varieties in order to obtain more balanced wines.

Is wine terroir a valid concept under a changing climate?

The OIV[i] defines terroir as a concept referring to an area in which collective knowledge of the interactions between the physical and biological environment (soil, topography, climate, landscape characteristics and biodiversity features) and vitivinicultural practices develops, providing distinctive wine characteristics. Those are perceptible in the taste of wine, which drives consumer preference and, therefore, wine’s value in the marketplace. Geographical indications (GI) are recognized regulatory constructs formalizing and protecting the nexus between wine taste and the terroir generating it. Despite considering updates, GIs do not consider the nexus as a dynamic one and do not anticipate change, namely of climate. Being climate a fundamental feature of terroir, it strongly impacts wine characteristics, such as taste. According to IPCC[ii], many widespread, rapid and unprecedented changes of climate occurred, some being irreversible over hundreds to thousands of years. Climatic shifts and atmospheric-driven extreme events have been widely reported worldwide. Recent climatic trends are projected to strengthen in upcoming decades, whereas extremes are expected to increase in frequency and intensity, forcing wines away from GI definitions. Geographical shifts of viticultural suitability are projected, often moving into regions and countries different from current ones. Some authors propose adaptation in viticulture, winemaking and product innovation. We show evidence of climate changing wine characteristics in the Douro valley, home of 270-year-old Port GI. We discuss herein resist or adapt stances for when climate changes the nexus between terroir and wine characteristics. Using the MED-GOLD[iii] dashboard, a tool allowing for easy visual navigation of past and future climates, we demonstrate how policymakers can identify future moments, throughout the 21st century under different emission scenarios, when GI specifications will likely need updates (e.g., boundaries, varieties) to reduce climate-change impacts.