IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Deciphering the color of rosé wines using polyphenol targeted metabolomics

Deciphering the color of rosé wines using polyphenol targeted metabolomics

Abstract

The color of rosés wines is extremely diverse  and a key element in their marketing. It is  due to the presence of red anthocyanins extracted from grape skins and pigments formed from them and other wine constituents during wine-making. To explore the link between composition and color, 268 commercial rosé wines were analyzed by ultra-high performance liquid chromatography coupled to triple quadrupole mass spectrometry analysis in the MRM (multiple reaction monitoring) mode [1] and their color characterized using spectrophotometry. The concentration of 125 phenolic compounds was thus determined and related to color parameters using chemometrics [2]. Color intensity is primarily determined by the extent of polyphenol extraction from the grapes. However, different compositions characterize the different color styles. Dark rosé wines contain high concentrations of anthocyanins and flavanols and their color, like that of red wines, is attributable to these molecules and their reaction products. In contrast, major phenolic compounds in light rosé wines are hydroxycinnamic acids and their salmon shade is mostly due to phenylpyranoanthocyanins and carboxypyranoanthocyanin pigments, resulting from reactions of anthocyanins, respectively with these phenolic acids and with pyruvic acid, a yeast metabolite. Redness of intermediate color wines is associated to anthocyanins and carboxypyranoanthocyanins while yellowness seems related to oxidation.The same approach was applied to monitor color and composition changes during fermentation of six rosé musts made from Grenache, Cinsault and Syrah grapes. Hydroxycinnamic acids were the major phenolic compounds in Grenache and Cinsault musts while the Syrah musts showed higher concentrations of anthocyanins and flavanols, indicating that polyphenol extraction is not only related to maceration conditions but also depends on varietal characteristics. These differences resulted in different proportions of derived pigments as observed on the rosé wine collection [2]. Comparison of the spectrophotometric and MRM data indicated that the majority of phenolic compounds in the Cinsault musts were not among the compounds targeted by MRM. Size exclusion chromatography (SEC) analysis of the musts showed different profiles for the three varieties, Cinsault musts containing large proportions of oligomeric compounds likely derived from hydroxycinnamates. These larger molecular weight compounds were no longer detected after fermentation and were partly recovered from the yeast lees. Comparison of the SEC profiles obtained at different wavelengths also suggest that pigments of Cinsault and Grenache are hydroxycinnamic acid derivatives, likely resulting from enzymatic oxidation. Non targeted metabolomics approaches provided further information on these pigments.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Cheynier, Véronique1, Leborgne Cécile2, Ducasse Marie-Agnès3, Meudec Emmanuelle1, Verbaere Arnaud1, Sommerer Nicolas1, Boulet Jean-Claude1, Masson Gilles2 and Mouret Jean-Roch11

SPO, INRAE, Univ Montpellier, Institut Agro, INRAE, PROBE research infrastructure, PFP Polyphenol Analytical Facility
2 SPO, INRAE, Univ Montpellier, Institut Agro, Montpellier, France; Institut Français de la Vigne et du Vin, Centre du rosé, Vidauban, France
3 Institut Français de la Vigne et du Vin, UMT OENOTYPAGE, Domaine de Pech Rouge, Gruissan, France

Contact the author

Keywords

UHPLC-MS/MS, polyphenols, rosé wines, color, chemometrics

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Effects of organic mulches on the soil environment and yield of grapevine

Farming management practices aiming at conserving soil moisture have been developed in arid and semiarid-areas facing water scarcity problems. Organic mulching is an effective method to manipulate the crop-growing microclimate increasing crop yield by controlling soil temperature, and retaining soil moisture by reducing soil evaporation. In this sense, the effectiveness of different organic mulching materials (straw mulch and grapevine pruning debris) applied within the row of a vineyard was evaluated on the soil and on the vine in a Tempranillo vineyard located in La Rioja (Spain). Organic mulches were compared with a traditional bare soil management technique (based on the use of herbicides to avoid weed incidence). Mulching coverages favourably influenced the soil water retention throughout all the grapevine vegetative cycle. However, the soil-moisture variation was not the same under different mulching materials, being the straw mulch (SM) the one that retained more water in comparison with grapevine pruning debris (GPD) based-cover. The changes of soil moisture in the upper surface layer (0–10 cm) were highly dynamic, probably due to water vapour fluxes across the soil-atmospheric interface. However, both, SM and GPD reduced these fluctuations as compared with bare soils. A similar trend occurred with soil temperature. Both organic mulches altered soil temperature in comparison with bare soil by reducing soil temperature in summer and raising it in winter. Moreover, the same buffering effect for the temperature on the covered soil also remains in the deeper layers. To conclude, we could see that organic mulching had a positive impact on soil-moisture storage and soil temperature and the extent of this effect depends on the type of mulching materials. These changes led to higher rates of photosynthesis and stomatal conductivity compared to bare soils, also favouring crop growth and grape yields.

Postveraison shoot trimming in Tannat and Merlot: preliminary results on yield components, plant balance and berry composition

There is currently a trend towards the production of wines with low alcohol content. To achieve this, grapes with low sugar content must be used. There are techniques at the vineyard level that can delay ripening and avoid excessive sugar accumulation without, a priori, affecting the final polyphenol content. Postveraison shoot trimming (PVST) is experimentally evaluated for these purposes, but its impact under Uruguayan climatic conditions with high interannual variability is not known. The aim of this work is to assess the PVST in Tannat and Merlot cultivars and their impact on yield components, plant balance and berry primary composition. In this study, two commercial vineyards of 10 years old Tannat and Merlot (grafted on SO4) at Canelones Department were selected. During the 2020-201 growing season, grapevines were submitted to PVST when grapes reached 15º Brix. In a randomized block, trimmed (T) and control (C) plants were evaluated with three repetitions each cultivar. Evaluation of the evolution of primary berry composition during ripening, measurement of yield components and plant balance were performed. For both cultivars, PVST did not affect yield components. Merlot reached 5.4 kg per plant and Tannat 7.1 kg, with not statistical significance between treatments. However, statistical differences were observed in terms of plant balance. In Merlot Ravaz Index reached a difference of 5.3 (12.0 in T and 6.7 in C) meanwhile Tannat reached 3.5 of statistical difference (13.7 in T and 10.2 in C). The tendency to imbalance for the treated plants had an impact on the final grape composition. Merlot grapes showed statistical difference in final total acidity (0.3 g of difference between treatments) while treatments impact final sugar content on Tannat grapes (10.0 g of difference between treatments). Further studies are needed to assess the impact of different canopy management techniques in our conditions.

Effects of graft quality on growth and grapevine-water relations

Climate change is challenging viticulture worldwide compromising its sustainability due to warmer temperatures and the increased frequency of extreme events. Grafting Vitis vinifera L.

Influence of agronomic practices in soil water content in mid-mountain vineyards

In the context of LIFE project MIDMACC (LIFE18 CCA/ES/001099), several pilots have been installed in vineyards in mid mountain areas of Catalonia (NE Spain) to test well stablished agronomic practices to increase the adaptation of Mediterranean mid mountain to climate change. Soil water content (SWC) at three different depths (15, 30 and 45cm) was measured in continuum from August 2020. One pilot (WC) included a well-established green cover (GC), a new GC (NC) and a conventional soil management (CM, tilling+herbicides). NC presented an intermediate state between WC and CM, responding similarly to CM in autumn but quickly reaching similar SWC to WC, then following the same evolution till next spring, with CM presenting lower values along autumn and winter. Then vegetation activation decreased SWC in all plots, (much slower in CM, lacking GC). Sensibility to spring rains is again intermediate for NC, which joins SWC evolution of CM by the end of spring till next autumn. It is expected that NC will resemble WC more and more as its GC develops. In the pilot combining vine training (VSP vs Gobelet) and hillside management (slope vs terrace), no clear pattern could be related with these conditions. However, both terraces seem to be more sensitive to spring rains. A third pilot included new vineyards (7 and 1 year old). In the new vineyard (N), higher canopy development, a spontaneous green cover and row straw resulted in a slower SWC dynamic, not so sensitive to rains but conserving more soil water in spring and most of summer, even with presumably a higher water extraction by vines. In the newest vineyard (VN) the deepest sensor is still sensitive to rain events all over the year and SWC is always highest at this depth, revealing small water capture by vines.

Elucidating vineyard site contributions to key sensory molecules: Identification of correlations between elemental composition and volatile aroma profile of site-specific Pinot noir wines

The reproducibility of elemental profile in wines produced across multiple vintages has been previously reported using grapes from a single scion clone of Vitis vinifera L. cv. Pinot noir. The grapevines were grown on fourteen different vineyard sites, from Oregon to southern California in the U.S.A., which span distances from approximately hundreds of meters to 1450 km, while elevations range from near sea level to nearly 500 m. In addition, sensorial (i.e. aroma, taste, and mouthfeel) and chemical (i.e. polyphenolic and volatile) differences across the different vineyard sites have also been observed among these wines at two aging time points. While strong evidence exists to support that grapes grown in different regions can produce wines with unique chemical and sensorial profiles, even when a single clone is used, the understanding of growing site characteristics that result in this reproducible differentiation continues to emerge. One hypothesis is that the elemental profile that a vineyard site imparts to the grape berries and the resulting wine is an important contributor to this differentiation in chemistry and sensory of wines. For example, various classes of enzymes that catalyze the formation of key aroma compounds or their precursors require specific metals. In this work, we begin to report correlations between elemental and volatile aroma profiles of site-specific Pinot noir wines, made under standardized winemaking conditions, that have been previously shown to be distinguished separately by these chemical analyses.