IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Deciphering the color of rosé wines using polyphenol targeted metabolomics

Deciphering the color of rosé wines using polyphenol targeted metabolomics

Abstract

The color of rosés wines is extremely diverse  and a key element in their marketing. It is  due to the presence of red anthocyanins extracted from grape skins and pigments formed from them and other wine constituents during wine-making. To explore the link between composition and color, 268 commercial rosé wines were analyzed by ultra-high performance liquid chromatography coupled to triple quadrupole mass spectrometry analysis in the MRM (multiple reaction monitoring) mode [1] and their color characterized using spectrophotometry. The concentration of 125 phenolic compounds was thus determined and related to color parameters using chemometrics [2]. Color intensity is primarily determined by the extent of polyphenol extraction from the grapes. However, different compositions characterize the different color styles. Dark rosé wines contain high concentrations of anthocyanins and flavanols and their color, like that of red wines, is attributable to these molecules and their reaction products. In contrast, major phenolic compounds in light rosé wines are hydroxycinnamic acids and their salmon shade is mostly due to phenylpyranoanthocyanins and carboxypyranoanthocyanin pigments, resulting from reactions of anthocyanins, respectively with these phenolic acids and with pyruvic acid, a yeast metabolite. Redness of intermediate color wines is associated to anthocyanins and carboxypyranoanthocyanins while yellowness seems related to oxidation.The same approach was applied to monitor color and composition changes during fermentation of six rosé musts made from Grenache, Cinsault and Syrah grapes. Hydroxycinnamic acids were the major phenolic compounds in Grenache and Cinsault musts while the Syrah musts showed higher concentrations of anthocyanins and flavanols, indicating that polyphenol extraction is not only related to maceration conditions but also depends on varietal characteristics. These differences resulted in different proportions of derived pigments as observed on the rosé wine collection [2]. Comparison of the spectrophotometric and MRM data indicated that the majority of phenolic compounds in the Cinsault musts were not among the compounds targeted by MRM. Size exclusion chromatography (SEC) analysis of the musts showed different profiles for the three varieties, Cinsault musts containing large proportions of oligomeric compounds likely derived from hydroxycinnamates. These larger molecular weight compounds were no longer detected after fermentation and were partly recovered from the yeast lees. Comparison of the SEC profiles obtained at different wavelengths also suggest that pigments of Cinsault and Grenache are hydroxycinnamic acid derivatives, likely resulting from enzymatic oxidation. Non targeted metabolomics approaches provided further information on these pigments.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Cheynier, Véronique1, Leborgne Cécile2, Ducasse Marie-Agnès3, Meudec Emmanuelle1, Verbaere Arnaud1, Sommerer Nicolas1, Boulet Jean-Claude1, Masson Gilles2 and Mouret Jean-Roch11

SPO, INRAE, Univ Montpellier, Institut Agro, INRAE, PROBE research infrastructure, PFP Polyphenol Analytical Facility
2 SPO, INRAE, Univ Montpellier, Institut Agro, Montpellier, France; Institut Français de la Vigne et du Vin, Centre du rosé, Vidauban, France
3 Institut Français de la Vigne et du Vin, UMT OENOTYPAGE, Domaine de Pech Rouge, Gruissan, France

Contact the author

Keywords

UHPLC-MS/MS, polyphenols, rosé wines, color, chemometrics

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

From a local to an international scale: sensory benchmarking of PDO wines. Quincy and Reuilly PDO wines (Sauvignon blanc) as a case study (France)

In a collective marketing strategy, the Protected Designation of Origin (PDO) can be used as a quality indicator. To highlight terroir specificities, it is useful to know how the wines are positioned on the local, national or international market from a sensory point of view. This is especially true for a comparison of varietal wines (e.g. Sauvignon blanc). We focus on the case of two closed Loire Valley PDO (France): Quincy and Reuilly. Three distinct tastings were organized. Firstly, at the local level comparing the 2 PDO (11 and 9 wines, 17 professional assessors); secondly at a regional level adding 3 closed PDO: Menetou-Salon, Sancerre and Pouilly-Fumé (3 wines per PDO, 16 assessors) and thirdly at an international level comparing these 5 PDO with Sauvignon Blanc wines coming from South Africa, New Zealand and Chile (1 to 3 wines per PDO, 19 assessors). All the wines were from the 2019 vintage and were considered to have a traditional elaboration process without contact with oak. A sensory descriptive analysis was performed using an aroma wheel allowing to combine a Check-All-That-Apply methodology, often used in sensory benchmarking, with a hierarchical structuration of the attributes. The aim is to facilitate data acquisition in a professional context without common training, to consider the hierarchical relationships among the attributes during the data analysis and to be able to characterize wines with a large range of sensorial variability. We use univariate, multivariate and clustering analyses. Similarities and differences between Quincy and Reuilly PDO wines and other Sauvignon blanc wines were identified. Specific attributes can distinguish the two PDO and different proximities exist with other local PDO, while clear differences were observed compared to international wines. Our study contributes to propose and discuss a method to do a wine sensory benchmarking highlighting sensory specificities linked to origin.

Analysis of Cabernet Sauvignon and Aglianico winegrape (V. vinifera L.) responses to different pedo-climatic environments in southern Italy

Water deficit is one of the most important effects of climate change able to affect agricultural sectors. In general, it determines a reduction in biomass production, and for some plants, as in the case of grapevine, it can endorse fruit quality. The monitoring and management of plant water stress in the vineyard

Co-design and evaluation of spatially explicit strategies of adaptation to climate change in a Mediterranean watershed

Climate change challenges differently wine growing systems, depending on their biophysical, sociological and economic features. Therefore, there is a need to locally design and evaluate adaptation strategies combining several technical options, and considering the local opportunities and constraints (e.g. water access, wine typicity). The case study took place in a typical and heterogeneous Mediterranean vineyard of 1,500 ha in the South of France. We developed a participatory modeling approach to (1) conceptualize local climate change issues and design spatially explicit adaptation strategies with stakeholders, (2) numerically evaluate their effects on phenology, yield and irrigation needs under the high-emissions climate change scenario RCP 8.5, and (3) collectively discuss simulation results. We organized five sets of workshops, with in-between modeling phases. A process-based model was developed that allowed to evaluate the effects of six technical options (late varieties, irrigation, water saving by reducing canopy size, adjusting cover cropping, reducing density, and shading) with various distributions in the watershed, as well as vineyard relocation. Overall, we co-designed three adaptation strategies. Delay harvest strategy with late varieties showed little effects on decreasing air temperature during ripening. Water constraint limitation strategy would compensate for production losses if disruptive adaptations (e.g. reduced density) were adopted, and more land got access to irrigation. Relocation strategy would foster high premium wine production in the constrained mountainous areas where grapevine is less impacted by climate change. This research shows that a spatial distribution of technical changes gives room for adaptation to climate change, and that the collaboration with local stakeholders is a key to the identification of relevant adaptation. Further research should explore the potential of adaptation strategies based on soil quality improvement and on water stress tolerant varieties.

The plantation frame as a measure of adaptation to climate change

The mechanization of vineyard work originally led to a reduction in planting densities due to the lack of machinery adapted to the vineyard. The current availability of specific machinery makes it possible to establish higher planting densities. In this work, three planting densities (1.40×0.80 m, 1.80×1 m and 2.20×1.20 m, corresponding to 8928, 5555 and 3787 plants/ha respectively) were studied with four varieties autochthonous of Galicia (northwestern Spain): Albariño and Treixadura (white), Sousón and Mencía (red). The vines were trained in a vertical shoot positioning system using a single Royat cordon, and pruned to spurs with two buds each. Agronomic data (yield, pruning wood weight, Ravaz index) and oenological data in must were collected. The higher planting density (1.40×0.80 m) had no significant effect on grape yield per vine in white varieties, although production per hectare was much higher due to the greater number of plants. In red varieties, this planting density resulted in a significantly lower production per vine, compensated by the greater number of plants. In addition, it significantly reduced the Brix degree in the must of the Albariño, Treixadura and Sousón varieties, and increased the total acidity in the latter two and Mencía. It also caused an increase in extractable and total anthocyanins and IPT in red grapes. The effects of high planting density on grapes are of great interest for the adaptation of varieties in the context of climate change. In the future, it could be advisable to modify the limits imposed by the appellations of origin on the planting density of these varieties in order to obtain more balanced wines.

Mapping and tracking canopy size with VitiCanopy

Understanding vineyard variability to target management strategies, apply inputs efficiently and deliver consistent grape quality to the winery is essential. However, despite inherent vineyard variability, the majority are managed as if they are uniform. VitiCanopy is a simple, grower-friendly tool for precision/digital viticulture that allows users to collect and interpret objective spatial information about vineyard performance. After four years of field and market research, an upgraded VitiCanopy has been created to achieve a more streamlined, technology-assisted vine monitoring tool that provides users with a set of superior new features, which could significantly improve the way users monitor their grapevines. These new features include:
• New user interface
• User authentication
• Batch analysis of multiple images
• Ease the learning curve through enhanced help features
• Reporting via the creation of colour maps that will allow users to assess the spatial differences in canopies within a vineyard.
Use-case examples are presented to demonstrate the quantification and mapping of vineyard variability through objective canopy measurements, ground-truthing of remotely sensed measurements, monitoring of crop conditions, implementation of disease and water management decisions as well as creating a history of each site to forecast quality. This intelligent tool allows users to manage grapevines and make informed management choices to achieve the desired production targets and remain profitable.