IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Deciphering the color of rosé wines using polyphenol targeted metabolomics

Deciphering the color of rosé wines using polyphenol targeted metabolomics

Abstract

The color of rosés wines is extremely diverse  and a key element in their marketing. It is  due to the presence of red anthocyanins extracted from grape skins and pigments formed from them and other wine constituents during wine-making. To explore the link between composition and color, 268 commercial rosé wines were analyzed by ultra-high performance liquid chromatography coupled to triple quadrupole mass spectrometry analysis in the MRM (multiple reaction monitoring) mode [1] and their color characterized using spectrophotometry. The concentration of 125 phenolic compounds was thus determined and related to color parameters using chemometrics [2]. Color intensity is primarily determined by the extent of polyphenol extraction from the grapes. However, different compositions characterize the different color styles. Dark rosé wines contain high concentrations of anthocyanins and flavanols and their color, like that of red wines, is attributable to these molecules and their reaction products. In contrast, major phenolic compounds in light rosé wines are hydroxycinnamic acids and their salmon shade is mostly due to phenylpyranoanthocyanins and carboxypyranoanthocyanin pigments, resulting from reactions of anthocyanins, respectively with these phenolic acids and with pyruvic acid, a yeast metabolite. Redness of intermediate color wines is associated to anthocyanins and carboxypyranoanthocyanins while yellowness seems related to oxidation.The same approach was applied to monitor color and composition changes during fermentation of six rosé musts made from Grenache, Cinsault and Syrah grapes. Hydroxycinnamic acids were the major phenolic compounds in Grenache and Cinsault musts while the Syrah musts showed higher concentrations of anthocyanins and flavanols, indicating that polyphenol extraction is not only related to maceration conditions but also depends on varietal characteristics. These differences resulted in different proportions of derived pigments as observed on the rosé wine collection [2]. Comparison of the spectrophotometric and MRM data indicated that the majority of phenolic compounds in the Cinsault musts were not among the compounds targeted by MRM. Size exclusion chromatography (SEC) analysis of the musts showed different profiles for the three varieties, Cinsault musts containing large proportions of oligomeric compounds likely derived from hydroxycinnamates. These larger molecular weight compounds were no longer detected after fermentation and were partly recovered from the yeast lees. Comparison of the SEC profiles obtained at different wavelengths also suggest that pigments of Cinsault and Grenache are hydroxycinnamic acid derivatives, likely resulting from enzymatic oxidation. Non targeted metabolomics approaches provided further information on these pigments.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Cheynier, Véronique1, Leborgne Cécile2, Ducasse Marie-Agnès3, Meudec Emmanuelle1, Verbaere Arnaud1, Sommerer Nicolas1, Boulet Jean-Claude1, Masson Gilles2 and Mouret Jean-Roch11

SPO, INRAE, Univ Montpellier, Institut Agro, INRAE, PROBE research infrastructure, PFP Polyphenol Analytical Facility
2 SPO, INRAE, Univ Montpellier, Institut Agro, Montpellier, France; Institut Français de la Vigne et du Vin, Centre du rosé, Vidauban, France
3 Institut Français de la Vigne et du Vin, UMT OENOTYPAGE, Domaine de Pech Rouge, Gruissan, France

Contact the author

Keywords

UHPLC-MS/MS, polyphenols, rosé wines, color, chemometrics

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Variations of soil attributes in vineyards influence their reflectance spectra

Knowledge on the reflectance spectrum of soil is potentially useful since it carries information on soil chemical composition that can be used to the planning of agricultural practices. If compared with analytical methods such as conventional chemical analysis, reflectance measurement provides non-destructive, economic, near real-time data. This paper reports results from reflectance measurements performed by spectroradiometry on soils from two vineyards in south Brazil. The vineyards are close to each other, are on different geological formations, but were subjected to the same management. The objective was to detect spectral differences between the two areas, correlating these differences to variations in their chemical composition, to assess the technique’s potential to predict soil attributes from reflectance data.To that end, soil samples were collected from ten selected vine parcels. Chemical analysis yield data on concentration of twenty-one soil attributes, and spectroradiometry was performed on samples. Chemical differences significant to a 95% confidence level between the two studied areas were found for six soil attributes, and the average reflectance spectra were separated by this same level along most of the observed spectral domain. Correlations between soil reflectance and concentrations of soil attributes were looked for, and for ten soil traits it was possible to define wavelength domains were reflectance and concentrations are correlated to confidence levels from 95% to 99%. Partial Least Squares Regression (PLSR) analyses were performed comparing measured and predicted concentrations, and for fifteen out of 21 soil traits we found Pearson correlation coefficients r > 0.8. These preliminary results, which have to be validated, suggest that variations of concentration in the investigated soil attributes induce differences in reflectance that can be detected by spectroradiometry. Applications of these observations include the assessment of the chemical content of soils by spectroradiometry as a fast, low-cost alternative to chemical analytical methods.

VINIoT: Precision viticulture service for SMEs based on IoT sensors network

The main innovation in the VINIoT service is the joint use of two technologies that are currently used separately: vineyard monitoring using multispectral imaging and deployed terrain sensors. One part of the system is based on the development of artificial intelligence algorithms that are feed on the images of the multispectral camera and IoT sensors, high-level information on water stress, grape ripening status and the presence of diseases. In order to obtain algorithms to determine the state of ripening of the grapes and avoid losing information due to the diversity of the grape berries, it was decided to work along the first year 2020 at berry scale in the laboratory, during the second year at the cluster scale and on the last year at plot scale. Different varieties of white and red grapes were used; in the case of Galicia we worked with the white grape variety Treixadura and the red variety Mencía. During the 2020 and 2021 campaigns, multispectral images were taken in the visible and infrared range of: 1) sets of 100 grapes classifying them by means of densimetric baths, 2) individual bunches. The images taken with the laboratory analysis of the ripening stage were correlated. Technological maturity, pH, probable degree, malic acid content, tartaric acid content and parameters for assessing phenolic maturity, IPT, anthocyanin content were determined. It has been calculated for each single image the mean value of each spectral band (only taking into account the pixels of interest) and a correlation study of these values with laboratory data has been carried out. These studies are still provisional and it will be necessary to continue with them, jointly with the training of the machine learning algorithms. Processed data will allow to determine the sensitivity of the multispectral images and select bands of interest in maturation.

Green berries on Gewürztraminer (Vitis vinifera L.) in South Tyrol (Italy)

The grape variety Gewürztraminer is known to be affected by two physiological disorders namely berry shrivel and bunch stem necrosis. During the season 2014 we noticed a new symptomatology type of ripening disorder on the variety. The new symptom showed not all berries fallowing the normal maturation stages, but single berries remaining at a soft but green stage till harvest. The broad distribution of these so called “green berries” symptoms in different production sites of our region, caused huge damage due to the difficulty of eliminating single berries per bunch before harvesting. Therefore, the Research Centre Laimburg began to investigate the reasons and origins of this new symptom. This work shows the results of first attempts to find causes for the symptom as well as the resulting approach to mitigate symptoms. Applications of magnesium leaf fertilizer showed first promising results against this putative disorder. To study the causal effect of the green berries 30 symptomatic vineyards in 2014 have been selected for a monitoring during the season 2016. To evaluate the foliar nutrient treatment two vineyards have been selected for application of magnesium sulfate and magnesium chloride. Leaf and berry nutrient analysis, as well as the main quality parameters during ripening have been performed. As soon as “green berries” symptoms appeared, incidence and severity have been evaluated. Most of the symptomatic vineyards of the 2016 monitoring showed light to clear magnesium deficit symptoms on their foliage. Only during the seasons 2020 and 2021 “green berries” symptoms could be found in the leaf fertilizer treatment vineyards. Both seasons showed a significant effect of the magnesium treatments to reduce the incidence and severity of the symptom. It seems that the appearance of the “green berries” symptom on Gewürztraminer is correlated to a disturbed uptake of magnesium of the vines.

Effects of organic mulches on the soil environment and yield of grapevine

Farming management practices aiming at conserving soil moisture have been developed in arid and semiarid-areas facing water scarcity problems. Organic mulching is an effective method to manipulate the crop-growing microclimate increasing crop yield by controlling soil temperature, and retaining soil moisture by reducing soil evaporation. In this sense, the effectiveness of different organic mulching materials (straw mulch and grapevine pruning debris) applied within the row of a vineyard was evaluated on the soil and on the vine in a Tempranillo vineyard located in La Rioja (Spain). Organic mulches were compared with a traditional bare soil management technique (based on the use of herbicides to avoid weed incidence). Mulching coverages favourably influenced the soil water retention throughout all the grapevine vegetative cycle. However, the soil-moisture variation was not the same under different mulching materials, being the straw mulch (SM) the one that retained more water in comparison with grapevine pruning debris (GPD) based-cover. The changes of soil moisture in the upper surface layer (0–10 cm) were highly dynamic, probably due to water vapour fluxes across the soil-atmospheric interface. However, both, SM and GPD reduced these fluctuations as compared with bare soils. A similar trend occurred with soil temperature. Both organic mulches altered soil temperature in comparison with bare soil by reducing soil temperature in summer and raising it in winter. Moreover, the same buffering effect for the temperature on the covered soil also remains in the deeper layers. To conclude, we could see that organic mulching had a positive impact on soil-moisture storage and soil temperature and the extent of this effect depends on the type of mulching materials. These changes led to higher rates of photosynthesis and stomatal conductivity compared to bare soils, also favouring crop growth and grape yields.

Variety and climatic effects on quality scores in the Western US winegrowing regions

Wine quality is strongly linked to climate. Quality scores are often driven by climate variation across different winegrowing regions and years, but also influenced by other aspects of terroir, including variety. While recent work has looked at the relationship between quality scores and climate across many European regions, less work has examined New World winegrowing regions. Here we used scores from three major rating systems (Wine Advocate, Wine Enthusiast and Wine Spectator) combined with daily climate and phenology data to understand what drives variation across wine quality scores in major regions of the Western US, including regions in California, Oregon and Washington. We examined effects of variety, region, and in what phenological period climate was most predictive of quality. As in other studies, we found climate, based mainly on growing degree day (GDD) models, was generally associated with quality—with higher GDD associated with higher scores—but variety and region also had strong effects. Effects of region were generally stronger than variety. Certain varieties received the highest scores in only some areas, while other varieties (e.g., Merlot) generally scored lower across regions. Across phenological stages, GDD during budbreak was often most strongly associated with quality. Our results support other studies that warmer periods generally drive high quality wines, but highlight how much region and variety drive variation in scores outside of climate.