IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Optimization Of Glutathione Extraction From White Wine Lees By Doelhert Matrix

Optimization Of Glutathione Extraction From White Wine Lees By Doelhert Matrix

Abstract

Glutathione (L-g-glutamyl-L-cysteinyl-glycine) is a tripeptide which contains three constitutive amino acids: glutamate, cysteine and glycine. It is present in plants and foods, and fruits like grapes. In must, wine or even yeast, glutathione can be found under its reduced (GSH) or oxidized form (GSSG) [1,2].  Many studies have proven that GSH plays a key role in wine quality and longevity [3]. It is well proved that during winemaking, particularly during wine aging on lees, the GSH concentration in wine increases and protects it from oxidation phenomena [4]. Nevertheless, the amount of GSH present in wine lees is often unknown and the choice of operating conditions (amount of lees and aging time) remains empirical. The aim of this study was to propose an optimized method to extract and to quantify the GSH potential of wine lees. In order to assess the main parameters affecting GSH extraction, the type of solvent, extraction time and solid-liquid ratio were investigated. A Response Surface Methodology (RSM) by Doehlert matrix, an useful tool for optimizing specific compound extraction, was applied to optimize GSH extraction from white lees. The results show that water is a suitable solvent for GSH extraction and that the solid-liquid ratio (< 15 g/L) and the extraction time (< 1h) are the main parameters that influence GSH extraction from lees. This approach was extended to the analysis of GSH present in enological product as yeast derivatives. This work in very helpful for developing a cost effective process for extraction of GSH from winemaking wastes as well as to analyze the GSH evolution in lees during winemaking in order to control operating condition of wine aging.

References

[1] Amir B.A.  and Ghobadi S., 2016. Studies on oxidants and antioxidants with a brief glance at their relevance to the immune system. Life Science, 146:163-73.
[2] Foyer C. and Noctor G., 2005 . Oxidant and antioxidant signalling in plants: a re-evaluation of the concept of oxidative stress in a physiological context. Plant Cell and Environemental, 28, 8: 1056-1071.
[3] Pons A., Lavigne V., Darriet P. and  Dubourdieu D., 2015. Glutathione preservation during winemaking with vitis vinifera white varieties: example of sauvignon blanc grapes. American Journal of Enology and Viticulture, 66- 2: 187-194.
[4] Lavigne, V. and Dubourdieu, D. 2002. Role of glutathione on development of aroma defects in dry white wines.  In 13th International Enology Symposium (Montpellier).

DOI:

Publication date: June 24, 2022

Issue: IVAS 2022

Type: Poster

Authors

Nioi Claudia1, Ren Yi1, Hastoy Xavier1 and Redon Pascaline 

1Institut des Sciences de la Vigne et du Vin, UMR OENOLOGIE (OENO)1366 Univ. Bordeaux, INRAE, Bordeaux INP

Contact the author

Keywords

Glutathione, Extraction, factorial design, Doelhert matrix, wine lees

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Measurement of redox potential as a new analytical winegrowing tool

Excell laboratory has initiated the development of an analytical method based on electrochemistry to evaluate the ability of wines to undergo or resist to oxidative phenomena. Electrochemistry is a powerful tool to probe reactions involving electron transfers and offers possibility of real-time measurements. In that context, the laboratory has implemented electrochemical analysis to assess oxidation state of different wine matrices but also in order to evaluate oxidative or reduced character of leaf and soil. Initially, our laboratory focused on dosage of compounds involved in responses of plant stresses and we were also interested in microbiological activity of soils. These analyses were compared with the measurement of redox potential (Eh) and pH which are two fundamental variables involved in the modulation of plant metabolism. Indeed, the variation of redox states of the plant reflects its biological activity but also its capacity to absorb nutriments. The Eh-pH conditions mainly determine metabolic processes involved in soil and leaf and our goal is to determine if this combined analytical approach will be sufficiently precise to detect biological evolutions (plant health, parasitic attack…).

Late frost protection in Champagne

Probably one of the most counterintuitive impacts of climate change on vine is the increased frequency of late frost. Champagne, due to its septentrional position is historically and regularly affected by this meteorological hazard. Champagne has therefore developed a strong experience in frost protection with first experiments dating from the end of 19th century. Frost protection can be divided in two parts: passive and active. Passive protection includes all the methods that do not seek to modify the vine’s environment or resistance at the time of frost. The most iconic passive protection in Champagne is the establishment of the individual reserve. This reserve allows to stock a certain quantity of clear wine during a surplus year to compensate a meteorological hazard like frost during the following years. Other common passive methods are the control of planting area (walls, bushes, topography), the choice of grape variety, late pruning, or the impact of grass cover and tillage. Active frost protection is also divided in two parts. Most of the existing techniques tend to modify vine’s environment. Most of the time they provide warmth (candles, heaters, windmills, heating cables…), or stabilise bud’s temperature above a lethal threshold (water sprinkling). The other way to actively fight is to enhance the resistance of buds to frost (elicitors). The Comité Champagne evaluates frost protection methods following three main axes: the efficiency, the profitability, and the environmental impact through a lifecycle assessment. This study will present the results on both passive and active protection following these three axes.

A predictive model of spatial Eca variability in the vineyard to support the monitoring of plant status

[lwp_divi_breadcrumbs home_text="IVES" use_before_icon="on" before_icon="||divi||400" module_id="publication-ariane" _builder_version="4.19.4" _module_preset="default" module_text_align="center" module_font_size="16px" text_orientation="center"...

Grape must quality and mesoclimatic variability in Fruška Gora wine-growing region, Serbia

The Fruška Gora mountain is a traditional wine-growing region in Serbia situated in the Pannonian Basin. Due to such a position, the vicinity of the Danube River and the presence of concave configuration, it is suitable for grape production. This paper provides analyses of spatial variations in meteorological parameters and grape juice quality within Fruška Gora wine region over three consecutive vintages (2018-2020). The examined period can be defined as warm with cool nights during September (AVG 18,9°C; GDD 1918°C; CI 12°CF) and with the presence of mesoclimatic variability. The East part of the study area was somewhat drier and hotter compared to other parts of the region. The analyses of grape must samples (190 in total) of five cultivars (Cabernet-Sauvignon, Merlot, Chardonnay, Sauvignon blanc and Grašac (Welschriesling)) commonly grown across the region (19 sites), were performed using Fourier Transform Infrared Technology (FTIR). Among all cultivars, Sauvignon blanc was harvested first in the East area (DOY=246±5, GDD at harvest=1552±74, 22.2±0.7 °Brix), while the latest harvest was recorded for Cabernet-Sauvignon in the West (DOY=283±5, GDD at harvest=1936±187, 23.4±1.0 °Brix ). Both the red and white cultivars had higher acidity and YAN in the grape must if the vines were grown in the North and East compared to South and West areas. According to PCA analysis, Grašac showed the lowest variation in grape must chemical composition. Thus, the results confirm that Grašac is the most stable cultivar in Fruška Gora. All monitored cultivars reached technological fruit ripeness by the end of the growing season. However, it was difficult to reach full ripeness of red cultivars, mostly beacuse of uncoupling of technolocical and phenolic ripeness. Thus, Cabernet-Sauvignon had higher variations in GDD sums at harvest compared to other cultivars, which probably increased variations in grape must quality.

Extreme canopy management for vineyard adaptation to climate change: is it a good idea?

Climate change constitutes an enormous challenge for humankind and for all human activities, viticulture not being an exception. Long-term strategic changes are probably needed the most, but growers also need to deal with short-term changes: summers that are getting progressively warmer, earlier harvest dates and higher pH in musts and wines. In the last 10-15 years, a relevant corpus of research is being developed worldwide in order to evaluate to which extent extreme canopy management operations, aimed at reducing leaf area and, thus, limiting the source to sink ratio, could be useful to delay ripening. Although extreme canopy management can result in relevant delays in harvest dates, longer term studies, as well as detailed analysis of their implications on carbohydrate reserves, bud fertility and future yield are desirable before these practices can be recommended.