IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 The influence of vine row position in terraced Merlot vineyards on water deficit and polyphenols – case study in the Vipava Valley, Slovenia

The influence of vine row position in terraced Merlot vineyards on water deficit and polyphenols – case study in the Vipava Valley, Slovenia

Abstract

A study was conducted in the Vipava Valley (Slovenia) to understand the effects of positioning rows of Merlot (Vitis vinifera L.) vines on terraces on plant available water, yield, and grape composition. Seven vineyards planted on dry, (hiper) skeletal Eutric Cambisol terrace slopes in a relatively limited area with similar mesoclimate were studied. The vines are planted in two rows: the inner rows, which are close to the slope and where the soil is more compact, are expected to have less available water, while the vines in the outer rows are more likely not to experience drought. Vineyard parameters (e.g., crop size, leaf area to yield ratio) were standardised in all selected vineyards as described by Sivilotti et al. (2020), and stem water potential (SWP) was measured during the growing season (Deloire and Heyns, 2011). Berry samples were randomly collected in triplicate from the inner and outer rows of the terraces at harvest time in 2019 and 2020. The skins and seeds were separated and extracted by accelerated extraction in organic solvents. The content of total anthocyanins (TA), free anthocyanins, total phenols (TP), proanthocyanidins (PAs), catechins and PAs reactive with vanillin (vanillin index) in grape skins and seeds was determined spectrophotometrically (Rigo et al., 2000). The structural characteristics of PAs (mean degree of polymerization (mDP), percent galloylation (%G), and percent prodelphinidins (%P)) in seeds and skins were analysed by UHPLC-DAD-MS /MS (Calderan et al., 2021). The profile of free anthocyanins in the skins was determined using HPLC-DAD (Vanzo et al., 2008). In early July 2019 at pre-verasion, statistically significant higher levels of SWP were measured on vines growing in the inner rows, while in late July and August, higher water deficit was measured in the outer rows of the terraces. The water deficit in early July 2019 was most likely related to the lower leaf area and yield in the inner rows. Grape seeds from inner rows contained more TP, vanillin reactive PAs, and PAs in dry weight and PAs had higher % G. Grape skins from inner rows contained more PAs in dry weight. On the other hand, grape skins from outer rows contained more TP, TA and more anthocyanidin 3-glucosides in fresh weight. In 2020 there was significantly higher SWP in late August and yield in the outer rows, and no differences in grape composition. The results indicated that the position of the vines on the terraces could influence the grape phenolic content and PAs structural characteristics. In this study, we confirmed differences in phenolic composition of Merlot grapes at the microscale within the vineyard, where the water deficit of the vines could have an important effect. Differences between row position of the vines suggest that it would be possible to improve the mechanical work with an excavator when establish new terraces in order to reduce water deficit of vines.

References

Calderan A., Sivilotti P., Braidotti R., Mihelčič A., Lisjak K., Vanzo A. 2021. Managing moderate water deficit increased anthocyanin concentration and proanthocyanidins galloylation in “Refošk” grapes in Northeast Italy. Agricultural water management, 246: 106684
Deloire A., Heyns D. 2011. The leaf potentials: Principles, method and thresholds. WineLand: 129-131
Rigo A., Vianello F., Clementi G., Rossetto M., Scarpa M., Vrhovšek U., Mattivi F. 2000. Contribution of proanthocyanidins to the peroxy radical scavenging capacity of some Italian red wines. Journal of agricultural and food chemistry, 48: 1996-2002
Sivilotti P., Falchi R., Vanderweide J., Sabbatini P., Bubola M., Vanzo A., Lisjak K., Peterlunger E., Herrera J. C. 2020. Yield reduction through cluster or selective berry thinning similarly modulates anhtocyanins and proanthocyanidins composition in Refosco dal peduncolo rosso (Vitis vinifera L.) grapes. Scientia Horticultura, 264: 1-9
Vanzo A., Terdoslavich M., Brandoni A., Torres A. M., Vrhovšek U., Passamonti S. 2008. Uptake of grape anthocyanins into the rat kidney and the involvement of bilitranslocase. Molecular nutrition and food research, 52: 1106-1116

DOI:

Publication date: June 24, 2022

Issue: IVAS 2022

Type: Poster

Authors

Mihelčič Alenka¹, Vanzo Andreja¹, Vrscaj Borut¹, Sivilotti Paolo² and Lisjak Klemen¹

1Agricultural Institute of Slovenia
2University of Udine

Contact the author

Keywords

Terraces, vine row position, stem water potential, grapes skin and seeds, polyphenols

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Characterization of variety-specific changes in bulk stomatal conductance in response to changes in atmospheric demand and drought stress

In wine growing regions around the world, climate change has the potential to affect vine transpiration and overall vineyard water use due to related changes in atmospheric demand and soil water deficits. Grapevines control their transpiration in response to a changing environment by regulating conductance of water through the soil-plant-atmosphere continuum. Most vineyard water use models currently estimate vine transpiration by applying generic crop coefficients to estimates of reference evapotranspiration, but this does not account for changes in vine conductance associated with water stress, nor differences thought to exist between varieties. The response of bulk stomatal conductance to daily weather variability and seasonal drought stress was studied on Cabernet-Sauvignon, Merlot, Tempranillo, Ugni blanc, and Semillon vines in a non-irrigated vineyard in Bordeaux France. Whole vine sap flow, temperature and humidity in the vine canopy, and net radiation absorbed by the vine canopy were measured on 15-minute intervals from early July through mid-September 2020, together with periodic measurement of leaf area, canopy porosity, and predawn leaf water potential. From this data, bulk stomatal conductance was calculated on 15-minute intervals, and multiple regression analysis was performed to identify key variables and their relative effect on conductance. Attention was focused on addressing multicollinearity and time-dependency in the explanatory variables and developing regression models that were readily interpretable. Variability of vapor pressure deficit over the day, and predawn water potential over the season explained much of the variability in conductance, with relative differences in response coefficients observed across the five varieties. By characterizing this conductance response, the dynamics of vine transpiration can be better parameterized in vineyard water use modeling of current and future climate scenarios.

Impact of changes in pruning practices on vine growth and yield

A gradual decline in vineyards has been observed over the past twenty years worldwide. This might be explained by the climate change, practices change or the increase of dieback diseases. To increase the longevity of vines, we studied the impact of different pruning strategies in four adult and four young vineyards located in France and Spain. In France, vineyards were planted with Cabernet franc on 3309C while Spanish trials were planted with Tempranillo grafted on 110R. Vegetative expression, yield, quality of berries and wood vessels conductivity were measured. The distribution of vegetative expression, yield and berry composition between primary and secondary vegetation were quantified. Finally, tomography was used to evaluate the implication of the treatments on sap flows.
First results show that i) the respectful pruning leads to an increase of 30 to 50% more secondary shoots than the aggressive pruning in France and between 15 and 20% in Spain, ii) there is no major effect on the yield over the first two years following the implementation of the new pruning practices, although the proportion of clusters from suckers is higher on the respectful pruning method. On young vines, the development of the trunk according to a respectful pruning leads to a loss of harvest 2 years after planting. This is due to the removal, on the future trunk, of the green suckers which carrying bunches. This operation carried out in spring rather than during winter pruning, would promote a better leaf / fruit balance when the plant comes into production, and could lead to better hydraulic conduction in the vessels of the trunk. Maintaining these trials for several years will provide more robust data to assess the impact of these practices on the vines over the long term.

Underpinning terroir with data: rethinking the zoning paradigm

Agriculture, natural resource management and the production and sale of products such as wine are increasingly data-driven activities. Thus, the use of remote and proximal crop and soil sensors to aid management decisions is becoming commonplace and ‘Agtech’ is proliferating commercially; mapping, underpinned by geographical information systems and complex methods of spatial analysis, is widely used. Likewise, the chemical and sensory analysis of wines draws on multivariate statistics; the efficient winery intake of grapes, subsequent production of wines and their delivery to markets relies on logistics; whilst the sales and marketing of wines is increasingly driven by artificial intelligence linked to the recorded purchasing behaviour of consumers. In brief, there is data everywhere!

Opinions will vary on whether these developments are a good thing. Those concerned with the ‘mystique’ of wine, or the historical aspects of terroir and its preservation, may find them confronting. In contrast, they offer an opportunity to those interested in the biophysical elements of terroir, and efforts aimed at better understanding how these impact on vineyard performance and the sensory attributes of resultant wines. At the previous Terroir Congress, we demonstrated the potential of analytical methods used at the within-vineyard scale in the development of Precision Viticulture, in contributing to a quantitative understanding of regional terroir. For this conference, we take this approach forward with examples from contrasting locations in both the northern and southern hemispheres. We show how, by focussing on the vineyards within winegrowing regions, as opposed to all of the land within those regions, we might move towards a more robust terroir zoning than one derived from a mixture of history, thematic mapping, heuristics and the whims of marketers. Aside from providing improved understanding by underpinning terroir with data, such methods should also promote improved management of the entire wine value chain.

Phenolic composition of Tempranillo Blanco grapes changes after foliar application of urea

Our research aimed to determine the effect and efficiency of foliar application of urea on the phenolic composition of Tempranillo Blanco grapes. The field experiment was carried out in 2019 and 2020 seasons and the plot was located in D.O.Ca Rioja (North of Spain). The vineyard was Vitis vinifera L. Tempranillo Blanco and grafted on Richter-110 rootstock. The treatments were control (C), whose plants were sprayed with water and three doses of urea: plants were sprayed with urea 3 kg N/ha (U3), 6 kg N/ha (U6) and 9 kg N/ha (U9). The applications were performed in two phenological stages, pre-veraison (Pre) and veraison (Ver). Also, each of the treatments was repeated one week later. Control and treatments were performed in triplicate and arranged in a randomised block design. Grapes were harvested at optimum ripening stage. High-performance liquid chromatography was used to analyse the phenolic composition of the grapes. Finally, the results obtained from the analytical determinations – flavonols, flavanols and non-flavonoid (hydroxybenzoic acids, hydroxycinnamic acids and stilbenes) – were studied statistically by analysis of variance. The results showed that, in 2019, U6-Pre and U9-Pre treatments increased the hydroxybenzoic acid content in grapes, and also all foliar treatments applied at Pre enhanced the stilbene concentration. Moreover, U3-Ver was the only treatment that rose flavonol and stilbene contents in the Tempranillo Blanco grapes. In 2020, all treatments applied at Pre enhanced the flavonol concentration in grapes. Furthermore, U3-Pre and U9-Pre treatments increased stilbene content in grapes. Nevertheless, the hydroxybenzoic acid content was improved by U6-Ver and U9-Ver and besides, hydroxycinnamic acid concentration in grapes was increased by all treatments applied at Ver. In conclusion, the lower and highest dose of urea (U3 and U9), applied at pre-veraison, were the best treatments to improve the Tempranillo Blanco grape phenolic composition.

Exploring resilience and competitiveness of wine estates in Languedoc-Roussillon in the recent past: a multi-level perspective

The Languedoc-Roussillon wineries are facing a decline in wine yields particularly PGI yields due to many factors. Climate change is just ones, but is expected to increase in the future. There is also structurally a large heterogeneity of yield profiles among terroirs, varieties and strategies. This work investigates the link between yield, competitiveness and resilience to explore how resilient winegrowers have been in the recent past. To this end two approaches have been combined; (i) an accountancy database analysis at estate scale and (ii) municipality level competitiveness analysis. A new resilience indicator that characterizes the capacity of an estate to absorb yield variation is also defined. The FADN database between 2000 and 2018 of ex-Languedoc-Roussillon (France) and other data are used to analyse the current situation and the past evolution of competitiveness and resilience by type of estate (type of farm: PGI and/or PDO & type of commercialization: bulk and/or bottles). The net margin, which defines competitiveness, is not correlated to yield for all types but depends on the type of commercialization and the level of specialisation. The resilience indicator shows that the net margin of estates specialized in PGI is particularly sensitive to yield declines. We also show that price evolutions seem to compensate the effect of yield losses for the majority of types. Municipality scale analysis shows the links between local pedoclimate, yield, commercialization strategies and price. Overlapping a PDO with a PGI does not always increase a municipality’s PGI competitiveness. It is difficult to make links between causes and effects due to the complexity of the wine production system. Production diversification may be a solution. Resorting to the two level of analysis helps resolving the data gap that is necessary to explore the links between yield and economic performance of the wine estates in the long term.