IVAS 2022 banner

Development, validation and application of a fast UHPLC-HRMS method for the analysis of amino acids and biogenic amines in wines and musts.

Abstract

The amino acids in grape juice are an important nitrogen source for yeast during alcoholic fermentation. Additionally, certain AAs are precursors to some of the volatile compounds found in wine and overall, they have an important role in the aromatic complexity of wines. Biogenic amines are produced during the fermentation process by microbial decarboxylation of the corresponding amino acid precursors. Yet, their fate is not only determined by the presence of microorganisms as they are also produced by the grape berries in response to abiotic factors. The presence of biogenic amines affect the sensory attributes of wines by reducing the varietal character and giving rise to meaty and metallic aromas in wines having higher pH values. Moreover, they also have a detrimental impact on consumer health. Due
to the importance of those compounds, several detection and quantification methods have been designed and published. However, to the best of our knowledge, none of them entailed the use of ultra-high performance liquid chromatography (UHPLC) coupled to a high-resolution mass spectrometry (HRMS). In this study, an innovative UHPLC-HRMS method useful for fast quantification of a broad range of amino acids and amines was developed. Twenty-five amino acids, twelve biogenic amines as well as glutathione and S-methylmethionine were identified and quantified in a single chromatographic run taking only 12 minutes. Additionally, a second run of the same length involving the use of o-phthalaldehyde derivatisation reagent was developed to quantify two more amines and ammonium. Validation of the method was performed in relation to the limit of detection, limit of quantification, linearity range, repeatability, reproducibility, and recovery. Once validated, the method was successfully tested on commercial oenological samples and grape musts, demonstrating its applicability to fast routine analysis of musts and wines

DOI:

Publication date: June 24, 2022

Issue: IVAS 2022

Type: Poster

Authors

Delaiti Simone¹, Nardin Tiziana¹and Larcher Roberto

¹Edmund Mach Foundation (FEM

Contact the author

Keywords

amino acids, amines, UHPLC-HRMS, wine, must

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Evaluation of winegrape anthocyanins in the vineyard using a portable fluorimetric sensor: seasonal and water regime effects

Accumulation of anthocyanins (Anth) on whole winegrape (Vitis vinifera L.) bunches attached to the vine was monitored by a fluorescence-based sensor (Multiplex) on ‘Aleatico’ and ‘Nero d’Avola’. Different water regimes were applied.

Grapevine nitrogen retrieval by hyperspectral sensing at the leaf and canopy level

Grapevine nitrogen (N) monitoring is essential for efficient N management plans that optimize fruit yield and quality while reducing fertilizer costs and the risk of environmental contamination. Unlike traditional vegetative-tissue sampling methods, remote sensing technologies, including hyperspectral imaging, have the potential to allow monitoring of the N status of entire vineyards at a per-vine resolution. However, differential N partitioning, variable spectral properties, and complex canopy structures hinder the development of a robust N retrieval algorithm. The present study aimed to establish a solid understanding of vine spectroscopic response at leaf and canopy levels by evaluating the different nitrogen retrieval approaches, including the radiative transfer model.

Irrigation as a tool for heatwave mitigation: the effect of irrigation intensity and timing in Cabernet Sauvignon

Heatwave events, defined as 2 or more days reaching ≥ 38 °C, are an increasingly frequent phenomenon threatening grape production worldwide. Heat stress has been shown to have negative consequences on grapevine physiology, leading to increased evaporative demand and intensified water stress. Due to heatwaves overlapping with important stages of grapevine reproductive development, spanning from berry set to the ripening stage, severe heat can potentially compromise yield and grape quality. The physiological response of grapevine to heat stress suggests a potential use of irrigation to mitigate heatwaves, however there is limited information regarding the irrigation amounts and timings needed for this purpose. Following up on a pivotal trial conducted between 2019 and 2022, in this study irrigation treatments with varying intensity and timing of application were refined to determine their potential mitigation of heat-associated damage to yield and fruit composition.

Soil management as a key factor on vineyard behavior under semiarid conditions: effects on soil biological activity, plant water and nutrient status, and grape yield and quality

Aims: Viticulture practices linked with soil management, as cover crops and deficit irrigation, can help to regulate the vineyard behavior reducing in most cases plant vigor and modifying plant water and nutrient status, and as a consequence, grape yield and quality. Also, these practices can modify the soil biological activity mostly related to microbiome diversity and functionality.

Evaluation de différents clones du Chardonnay pendant la maturation dans un terroir viticole du Friuli-Venezia Glulia (Nord-Est de l’Italie)

La diffusion récente et “explosive” du Chardonnay dans pratiquement toutes les zones de culture viticole du monde a fait penser, à tort, que cette variété s’adapte facilement à toutes les conditions pédo-climatiques ou presque. Cette thèse a été confirmée par la grande faculté d’adaptation dont a fait preuve le vignoble et par la popularité dont jouit le vin auprès des consommateur du monde entier.