IVAS 2022 banner

Development, validation and application of a fast UHPLC-HRMS method for the analysis of amino acids and biogenic amines in wines and musts.

Abstract

The amino acids in grape juice are an important nitrogen source for yeast during alcoholic fermentation. Additionally, certain AAs are precursors to some of the volatile compounds found in wine and overall, they have an important role in the aromatic complexity of wines. Biogenic amines are produced during the fermentation process by microbial decarboxylation of the corresponding amino acid precursors. Yet, their fate is not only determined by the presence of microorganisms as they are also produced by the grape berries in response to abiotic factors. The presence of biogenic amines affect the sensory attributes of wines by reducing the varietal character and giving rise to meaty and metallic aromas in wines having higher pH values. Moreover, they also have a detrimental impact on consumer health. Due
to the importance of those compounds, several detection and quantification methods have been designed and published. However, to the best of our knowledge, none of them entailed the use of ultra-high performance liquid chromatography (UHPLC) coupled to a high-resolution mass spectrometry (HRMS). In this study, an innovative UHPLC-HRMS method useful for fast quantification of a broad range of amino acids and amines was developed. Twenty-five amino acids, twelve biogenic amines as well as glutathione and S-methylmethionine were identified and quantified in a single chromatographic run taking only 12 minutes. Additionally, a second run of the same length involving the use of o-phthalaldehyde derivatisation reagent was developed to quantify two more amines and ammonium. Validation of the method was performed in relation to the limit of detection, limit of quantification, linearity range, repeatability, reproducibility, and recovery. Once validated, the method was successfully tested on commercial oenological samples and grape musts, demonstrating its applicability to fast routine analysis of musts and wines

DOI:

Publication date: June 24, 2022

Issue: IVAS 2022

Type: Poster

Authors

Delaiti Simone¹, Nardin Tiziana¹and Larcher Roberto

¹Edmund Mach Foundation (FEM

Contact the author

Keywords

amino acids, amines, UHPLC-HRMS, wine, must

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Impact of climate variability and change on grape yield in Italy

Viticulture is entangled with weather and climate. Therefore, areas currently suitable for grape production can be challenged by climate change. Winegrowers in Italy already experiences the effect of climate change, especially in the form of warmer growing season, more frequent drought periods, and increased frequency of weather extremes.
The aim of this study is to investigate the impact of climate variability and change on grape yield in Italy to provide winegrowers the information needed to make their business more sustainable and resilient to climate change. We computed a specific range of bioclimatic indices, selected by the International Organisation of Vine and Wine (OIV), and correlated them to grape yield data. We have worked in collaboration with some wine consortiums in northern and central Italy, which provided grape yield data for our analysis.
Using climate variables from the E-OBS dataset we investigate how the bioclimatic indices changed in the past, and the impact of this change on grape productivity in the study areas. The climate impact on productivity is also investigated by using high-resolution convection-permitting models (CPMs – 2.2 horizontal resolution), with the purpose of estimating productivity in future emission scenarios. The CPMs are likely the best available option for this kind of impact studies since they allow a better representation of small-scale processes and features, explicitly resolve deep convection, and show an improved representation of extremes. In our study, we also compare CPMs with regional climate models (RCMs – 12 km horizontal resolution) to assess the added value of high-resolution models for impact studies. Further development of our study will lead to assessing the future suitability for vine cultivation and could lead to the construction of a statistical model for future projection of grape yield.

Response of Shiraz/101‐14 mgt to in‐row vine spacing

Knowledge of vine reaction to plant spacing under high potential soil conditions is restricted. This study was done to determine effects of vine spacing

Monitoring gas-phase CO2 in the headspace of champagne glasses through diode laser spectrometry

During Champagne or sparkling wine tasting, gas-phase CO2 and volatile organic compounds invade the headspace above glasses [1], thus progressively modifying the chemical space perceived by the consumer. Gas-phase CO2 in excess can even cause a very unpleasant tingling sensation perturbing both ortho- and retronasal olfactory perception [2]. Monitoring as accurately as possible the level of gas-phase CO2 above glasses is therefore a challenge of importance aimed at better understanding the close relationship between the release of CO2 and a collection of various tasting parameters.

Rapid measurement of phenolic quality as a useful tool for viticultural zoning

Un des principaux objectifs du zonage viticole est l’individuation des zones plus indiquées à la production de vins de haute qualité en relation aux cépages. Ceperrlant depuis beaucqup d’années, entre les paramètres de qualité du raisin, on n’a pas considéré les substances phénoliques par effet de l!l difficulté d’analyse en temps rapides.

How are canned wine drinkers perceived? An investigation involving Swiss nationals and different scenarios of outdoor leisure activities

This study examines how people who consume wine in cans are perceived in terms of their basic personality characteristics, helps understand the role of cultural background on people’s perception, and verify the role played by the consumption context on the perception. Our hypothesis is that prejudice and negative attitudes towards wine in cans might exert a negative effect on the evaluation of people who consume canned wine. To evaluate this hypothesis, the consumption of wine in cans was evoked in four different contexts of use during outdoor leisure activity (beach resort, ski resort, desert safari, and party). In order to examine the effect of culture on subject’s response we use participants from Switzerland, a country where three different cultures, associated with three different languages, cohabit.