Development, validation and application of a fast UHPLC-HRMS method for the analysis of amino acids and biogenic amines in wines and musts.

The importance of the non-Saccharomyces yeasts (NSY) in winemaking has been extensively reviewed in the past for their aromatic or bioprotective capacity while, recently their antioxidant/antiradical potential has emerged under winemaking conditions. In the literature the antioxidant potential of NSY was solely explored through their capacity to improve glutathione (GSH) content during alcoholic fermen- tation [1], while more and more studies pointed out the activity of the non-glutathione soluble fraction released by yeasts [2].

In this work, these four red varieties were characterized in terms of phenolic composition. Thus, the anthocyanin accumulation and the extractability evolution during ripening were compared.

The development of interspecific hybrid varieties (ihvs) resistant to diseases such as powdery mildew and downy mildew allows for a decrease in the use of inputs in vineyards. In this pers-pective, ihvs represent a response to societal demand for reducing environmental impact and are increasingly used in viticulture. At the same time, wines resulting from so-called sponta-neous fermentations, based on indigenous flora, have recently gained popularity.

Over the last decade, the use of non-Saccharomyces yeasts in the winemaking process has been re-assessed and accepted by winemakers. These yeasts can be used to achieve specific objectives such as lowering the ethanol content, preventing wine spoilage and increasing the production of specific aroma compounds. Since these species are unable to complete alcoholic fermentation, strategies of co- and sequential inoculation of non-Saccharomyces and Saccharomyces cerevisiae have been developed. However, when mixed starter cultures are used, several parameters (e.g. strain yeast, inoculation timing and nutrient competitions) impact the growth of the individual yeasts, the fermentation kinetics and the metabolites/aroma production. In particular, competition for nitrogen compounds could have a major impact, potentially leading to sluggish fermentation when the yeast assimilable nitrogen (YAN) availability is low. Moreover, many aroma compounds produced by the yeasts are directly produced and influenced by nitrogen metabolism such as higher alcohols, acetate esters and ethyl esters which participate in the organoleptic complexity of wine.

In the Cognac region in France, Ugni blanc is the most planted grape variety (98% of the 80 500 ha). This vine region is in expansion due to the success of the associated well-known brandy and the need of high grape yield to guarrantee the production of base wine for distillation. About 2 to 3000 ha are newly planted each year and rootstocks are one powerfull tool for vineyard adaptation to soil or climate change. As rootstocks ensure water and mineral nutrient supplies to the scion, it is important to better understand their effect on berry compostionnal parameters such as sugars and nitrogen compounds, which are the main precursors for fermentary aroma metabolites, the latter being quality markers for Cognac after distillation.