IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Effect of the plant sink/source balance on the chemical content of red table grapes (Vitis vinifera L.).

Effect of the plant sink/source balance on the chemical content of red table grapes (Vitis vinifera L.).

Abstract

PPhloem transport of assimilates provides the materials needed for the growth and development of reproductive structures, storage and developing organs, and has long been recognized as a major determinant in crop yield. Thus, the understanding of the mechanisms and regulations of sugar transport into sink tissues has an important basic and applied relevance. The grapevine is a good example of a crop where sugar accumulation in the fruit has an important economic role. Massive sugar transport and compartmentation into the grape berry mesocarp cells (up to 1 M glucose and fructose) start at veraison and continues until the harvest. Sucrose transported in the phloem is cleaved into hexoses by invertases and stored in the vacuole. The sugar content determines the sweetness of table grapes and regulates gene expression, including, for example, several genes involved in the synthesis of secondary metabolites which contribute to grape quality. Many viticultural practices affect source/sink relationships, thus altering sugar concentration in the berry.  Considering this, the aim of this work was determined the effect of change of source/sink relations by using treatments of cluster thinning, cane girdling and leaf removal, on anthocyanin and volatile composition in grape berries during ripening. Berry grapes from varieties Red Globe and TimcoTM, cultivated in the same place under the 3 agronomical treatments (cluster thinning, cane girdling and leaf removal) were sampled from veraison to commercial maturity each ≈10-12 days. The anthocyanin composition was analyzed spectrophotometrically and by HPLC-DAD [1]. The volatile profiles from grapes were analyzed employing for the extraction solid-phase microextraction and gas chromatography coupled with mass spectrometry [2]. For both varieties, cluster thinning affect the concentration of total soluble solids and some anthocyanins in relation with the control, increasing their concentration. In relation with volatile compounds forty-one volatile compounds were determined in the two grape varieties analyzed. Among them, the terpene chemical group was the most abundant (qualitatively), accounting for 14 compounds, followed by aldehydes (13), alcohols (9), ketones (3), C13-norisoprenoids (1), and acids (1). Both varieties presented a different behavior in the evolution for the total volatiles during ripening, with a decrease during ripening for Red Globe and an increase in the case of TimcoTM berries. Except for esters with an increase in the treatment with cluster thinning and cane girdling respect the control for TimcoTM samples at commercial maturity, no effect was observed for the rest of the volatile chemical groups among the treatments. Data suggest that use of some of the plant management practices studied may improve berry color, but with a very low and/or inconsistent impact of the plant sink/source on the berry volatile fraction.

References

[1].Cortiella, MG; Ubeda, C; del Barrio-Galan, R; Pena-Neira, A. 2020. Impact of berry size at harvest on red wine composition: a winemaker’s approach. Journal of the Science of Food and agriculture. 100(2):836-845
[2]. Ubeda, C.; Gil i Cortiella, M.; Villalobos-González, L.; Gómez, C.
Pastenes, C.; Peña-Neira, Á. 2020. Ripening and Storage Time Effects on the Aromatic Profile of New Table Grape Cultivars in Chile. Molecules, 25(24), 5790.

DOI:

Publication date: June 24, 2022

Issue: IVAS 2022

Type: Poster

Authors

Peña-Neira Alvaro1, Vega Rommyna1, Gil i Cortella Mariona2, Gomez-Celis Camila1, Ubeda-Aguilera Cristina2, Villalobos Luis3 and Pastenes Claudio3

1Departamento de Agroindustria y Enología. Facultad de Ciencias Agronómicas, Universidad de Chile.
2Instituto de Ciencias Químicas Aplicadas, Inorganic Chemistry and Molecular Material Center, Facultad de Ingeniería, Universidad Autónoma de Chile
3Departamento de Producción Agrícola. Facultad de Ciencias Agronómicas, Universidad de Chile. Santa Rosa 11315, Santiago, Chile.

Contact the author

Keywords

Red Globe; TimcoTM; phenolic compounds; aroma; anthocyanins.

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Use of a new, miniaturized, low-cost spectral sensor to estimate and map the vineyard water status from a mobile 

Optimizing the use of water and improving irrigation strategies has become increasingly important in most winegrowing countries due to the consequences of climate change, which are leading to more frequent droughts, heat waves, or alteration of precipitation patterns. Optimized irrigation scheduling can only be based on a reliable knowledge of the vineyard water status.

In this context, this work aims at the development of a novel methodology, using a contactless, miniaturized, low-cost NIR spectral tool to monitor (on-the-go) the vineyard water status variability. On-the-go spectral measurements were acquired in the vineyard using a NIR micro spectrometer, operating in the 900–1900 nm spectral range, from a ground vehicle moving at 3 km/h. Spectral measurements were collected on the northeast side of the canopy across four different dates (July 8th, 14th, 21st and August 12th) during 2021 season in a commercial vineyard (3 ha). Grapevines of Vitis vinifera L. Graciano planted on a VSP trellis were monitored at solar noon using stem water potential (Ψs) as reference indicators of plant water status. In total, 108 measurements of Ψs were taken (27 vines per date).

Calibration and prediction models were performed using Partial Least Squares (PLS) regression. The best prediction models for grapevine water status yielded a determination coefficient of cross-validation (r2cv) of 0.67 and a root mean square error of cross-validation (RMSEcv) of 0.131 MPa. This predictive model was employed to map the spatial variability of the vineyard water status and provided useful, practical information towards the implementation of appropriate irrigation strategies. The outcomes presented in this work show the great potential of this low-cost methodology to assess the vineyard stem water potential and its spatial variability in a commercial vineyard.

The impact of sustainable management regimes on amino acid profiles in grape juice, grape skin flavonoids, and hydroxycinnamic acids

One of the biggest challenges of agriculture today is maintaining food safety and food quality while providing ecosystem services such as biodiversity conservation, pest and disease control, ensuring water quality and supply, and climate regulation. Organic farming was shown to promote biodiversity and carbon sequestration, and is therefore seen as one possibility of environmentally friendly production. Consumers expect organically grown crops to be free from chemical pesticides and mineral fertilizers and often presume that the quality of organically grown crops is different or higher compared to conventionally grown crops. Integrated, organic, and biodynamic viticulture were compared in a replicated field trial in Geisenheim, Germany (Vitis vinifera L. cv. Riesling). Amino acid profiles in juice, grape skin flavonoids, and hydroxycinnamic acids were monitored over three consecutive seasons beginning 7 years after conversion to organic and biodynamic viticulture, respectively. In addition, parameters such as soil nutrient status, yield, vigor, canopy temperature, and water stress were monitored to draw conclusions on reasons for the observed changes. Results revealed that the different sustainable management regimes highly differed in their amino acid profiles in juice and also in their skin flavonol content, whereas differences in the flavanol and hydroxycinnamic acid content were less pronounced. It is very likely that differences in nutrient status and yield determined amino acid profiles in juice, although all three systems showed similar amounts of mineralized nitrogen in the soil. Canopy structure and temperature in the bunch zone did not differ among treatments and therefore cannot account for the observed differences in favonols. A different light exposure of the bunches in the respective systems due to differences in vigor together with differences in berry size and a different water status of the vines might rather be responsible for the increase in flavonol content under organic and biodynamic viticulture.

Vineyards and clay minerals: multi-technique analytical approach and correlations with soil properties

Purpose of this research is to quantitatively assess the mineral component of vineyard soils, with particular attention to the mineralogical analysis of clays, which represent an element of high importance in the vineyard culture as well as in general agriculture. An X-ray diffraction (XRD) / thermogravimetric (TG) multi-technique analytical approach was developed, tested on soil samples taken from vineyards around the world. This codified analytical procedure was necessary to obtain precise qualitative and quantitative mineralogical data, globally comparable to distinguish the geopedological identity of the vineyards. Soil samples from vineyards of various locations were analysed, in very different geological conditions. The bulk-rock quantitative phase analysis (QPA) was obtained by the Rietveld method while the detailed composition of the clay-sized fraction was determined by modelling of the oriented X-ray diffraction patterns. The research provided a precise classification of the mineral component of soils, distinguishing the mineral phases of the clays and the so-called mixed-layer clay minerals. We found that the content in mixed layers can be directly correlated with the water retention and the cation exchange capacity ​​of the soil, while the presence of other clayey minerals and phyllosilicates in this research did not affect this CEC parameter, which codes the fertility level of the soils. The study demonstrates that terroir, in particular soils formed in complex or very different geological conditions, can only be effectively interpreted by properly analysing its mineral phases, in particular the mixed-layer clay component. These are characteristic abiotic ecological indicators, which may have specific eco-physiological influences on the plant.

Phenological characterization of a wide range of Vitis Vinifera varieties

In order to study the impact of climate change on Bordeaux grape varieties and to assess the adaptation capacities of candidates to the grape varieties of this wine region to the new climatic conditions, an experimental block design composed of 52 grape varieties was set up in 2009 at the INRAE Bordeaux Aquitaine center. Among the many parameters studied, the three main phenological stages of the vine (budburst, flowering and veraison) have been closely monitored since 2012. Observations for each year, stage and variety were carried out on four independent replicates. Precocity indices have been calculated from the data obtained over the 2012-2021 period (Barbeau et al. 1998). This work allowed to group the phenological behaviour of the grapevine varieties, not only based on the timing of the subsequent developmental stages, but also on the overall precocity of the cycle and the total length of the cycle between budburst and veraison. Results regarding the variability observed among the different grape varieties for these phenological stages are presented as heat maps.

Impact of yeast derivatives to increase the phenolic maturity and aroma intensity of wine

Using viticultural and enological techniques to increase aromatics in white wine is a prized yet challenging technique for commercial wine producers. Equally difficult are challenges encountered in hastening phenolic maturity and thereby increasing color intensity in red wines. The ability to alter organoleptic and visual properties of wines plays a decisive role in vintages in which grapes are not able to reach full maturity, which is seen increasingly more often as a result of climate change. A new, yeast-based product on the viticultural market may give the opportunity to increase sensory properties of finished wines. Manufacturer packaging claims these yeast derivatives intensify wine aromas of white grape varieties, as well as improve phenolic ripeness of red varieties, but the effects of this application have been little researched until now. The current study applied the yeast derivative, according to the manufacture’s instructions, to the leaves of both neutral and aromatic white wine varieties, as well as on structured red wine varieties. Chemical parameters and volatile aromatics were analyzed in grape musts and finished wines, and all wines were subjected to sensory analysis by a tasting panel. Collective results of all analyses showed that the application of the yeast derivative in the vineyard showed no effect across all varieties examined, and did not intensify white wine aromatics, nor improve phenolic ripeness and color intensity in red wine.