IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Lactiplantibacillus plantarum – A versatile tool for biological deacidification

Lactiplantibacillus plantarum – A versatile tool for biological deacidification

Abstract

Malolactic fermentation (MLF) is a secondary wine fermentation conducted by lactic acid bacteria (LAB). This fermentation is important in winemaking as it deacidifies the wine, converting L-malic acid into L-lactic acid and carbon dioxide, and it contributes to microbial stability. Wine pH is highly selective, and at pH below 3.5 generally only strains of O. oeni can survive and express malolactic activity, while under more favorable growth conditions above pH 3.5, species of Lactobacillus and Pediococcus may conduct the MLF. Among the LAB species Lactiplantibacillus plantarum strains have shown most interesting results under hot climate conditions, not only for their capacity to induce MLF, but also for their homo-fermentative properties towards hexose sugars, which makes them suitable for induction of MLF in high pH and high alcohol wines, when inoculated at the beginning of alcoholic fermentation.
Recently a highly concentrated L. plantarum starter culture proofed not only being able to induce and finish a malolactic fermentation before the end of alcoholic fermentation, when applied in co-inoculation in high pH red wines, but also to be a tool for high acidic white wines, characterized by a low pH (> pH 2.95) and high malic acid concentrations. Due to its good alcohol tolerance (up to 15 %vol) it can be applied in co-inoculation as well as in sequential inoculation.
An inoculation ratio could be used to control the amount of malic acid to be degraded to achieve both: a partial or a complete degradation of malic acid. Since this strain does not metabolize citric acid, no diacetyl is formed and thus the variety typicity is maintained and wine acidity is harmonized. With the partial or complete removal of the malic acid, the complex double salt or in some cases even necessary extended double salt deacidification could be circumvented. This also avoided calcium input and eliminated the resulting problems with tartar stabilization.
For use in sequential inoculation, a simple MLF pretest can be used to determine the success of an MLF with ML-Prime in a short time (maximum 7 days) and to provide exact information which amount of malic acid will be degraded. The results can be transferred directly into practice.

DOI:

Publication date: June 27, 2022

Issue: IVAS 2022

Type: Poster

Authors

Krieger-Weber Sibylle1

1Lallemand Office Korntal-Münchingen

Contact the author

Keywords

Malolactic fermentation; Lactiplantibacillus plantarum; facultative hetero-fermentative; starter cultures; diacetyl.

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

The effects of canopy side on the chemical composition of merlot, Cabernet-Sauvignon, and Carmenère (Vitis vinifera L.) Grapes during ripening

Carmenère fruit during ripening of a Vertical shoot positioning, VSP, trained experimental vineyard with north-south row orientation.

Modulation of berry composition by different vineyard management practices

High concentration of sugars in grapes and alcohol in wines is one of the consequences of climate change on viticulture production in several wine-growing regions. In order to investigate the possibilities of adaptation of vineyard management practices aimed to reduce the accumulation of sugar during the maturation phase without reducing the accumulation of anthocyanins in grapes, a study with severe shoot trimming, shoot thinning, cluster thinning and date of harvest was conducted on Merlot variety in Istria region (Croatia), under the Mediterranean climate. Four factors which may affect grape maturation and its composition at harvest were investigated in a two-years experiment; severe shoot trimming applied at veraison when >80% of berries changed colour (in comparison to untreated control), shoot thinning (0 and 30%), cluster thinning (0 and 30%), and the date of harvest (early and standard harvest dates). Shoot thinning had no significant impact on berry composition, despite the obtained reduction in yield per vine. Lower Brix in grapes were obtained with earlier harvest date and if no cluster thinning was applied, although at the same time a reduction in the concentration of anthocyanins in berries was observed in these treatments. On the other hand, if severe shoot trimming was applied when >80% of berries changed colour, a reduction of Brix was obtained without a negative impact on berry anthocyanins concentration. We conclude that in cases when undesirably high sugar concentrations at harvest are expected, severe shoot trimming at 80% veraison may effectively be used in order to obtain moderate sugar concentration in berries together with the adequate phenolic composition.

Vitis vinifera ‘Ráthay’ on the rootstocks ‘Kober 5BB’, ‘Fercal’, and ‘3309 C’: results of a long-term field trial

Context and purpose of the study. Ráthay is an Austrian red quality wine variety with increased resistance to fungi.

Towards adaptation to climate change in Rioja: Quality evaluation of wines obtained from Grenache x Tempranillo selections

The wine sector is of great relevance and tradition in Mediterranean countries, however, it may be most susceptible to climate change. In recent years, wine production is facing changes worldwide, both at environmental as well as commercial levels, due to global warming and the shift in consumers’ preferences. Wine growers and wine makers are in search of solutions that allow to face these new challenges. One of the most promising initiatives in the long term is the introduction of new plant materials, specifically intraspecific hybridizations between premium varieties that may improve traditional germplasm in its adaptation to climate change. These inter-varietal crosses have the potential to generate quality wines, whilst maintaining the regional typicity, and constitute an attractive alternative for the consumer due to their sensory attributes. In this study, we have evaluated wines from 29 intraspecific Garnacha x Tempranillo hybrids in two different locations, with the aim to assess their oenological potential and sensory attributes. Thirteen of the selections were white and 16 were red. Microvinifications were conducted with two or three replications depending on grape availability. Conventional oenological parameters were determined for all wines. The sensory evaluation and hedonic scores were given by five experts. Red selections obtained higher quality scores than white ones. Among the white selections with higher quality scores, GT-41 Varea and GT-159 Varea outstand, due to their high total acidity and high malic acid content. Regarding red selections, GT-57 Varea and GT-57 UR were perceived as higher in quality, highlighted for their moderate alcoholic and high anthocyanin content. Our results indicate that intraspecific hybridization may be a powerful tool for adapting traditional cultivars to climate change in Rioja.

Importance des propriétés optiques de la surface du sol sur le microclimat de la vigne. Répercussions de l’usage d’un revêtement de sol réfléchissant sur la composition des moûts et sur la qualité du vin

Cette recherche a eu pour but l’étude des effets d’un renforcement radiatif et thermique sur les zones inférieures de la canopée de la vigne (solarisation par des films ou des paillages réfléchissants installés sur le sol, sous les ceps), notamment l’étude de leurs conséquences sur la composition biochimique des moûts à la vendange et sur la qualité des vins.