terclim by ICS banner
IVES 9 IVES Conference Series 9 GiESCO 9 GiESCO 2023 9 Identification of arbuscular mycorrhizal fungi species preferentially associated with grapevine roots inoculated with commercial bioinoculants 

Identification of arbuscular mycorrhizal fungi species preferentially associated with grapevine roots inoculated with commercial bioinoculants 


Context and purpose of the study – Arbuscular mycorrhizal fungi (AMF) form symbiotic associations with plant roots and can help plants acquire nutrients from the soil in exchange for photosynthetic carbon. Commercial bioinoculants containing AMF are widely available and represent a potential opportunity to reduce the dependence of grapevines on agrochemicals. However, which commercially available AMF species colonize vine roots and affect vine growth remains unknown. The aim of this study was to identify the AMF species from commercial bioinoculants that colonize grapevine roots using high-throughput sequencing, and to evaluate the performance of five commercial bioinoculants and their effects on own-rooted Cabernet sauvignon.

Material and methods – Two-year-old own-rooted Cabernet sauvignon vines were potted into a non-sterile orchard-collected soil and placed in a greenhouse. The silt loam soil was low in available nitrogen, phosphorus, potassium and organic matter and had a neutral pH.  Pots were inoculated with one of five commercial bioinoculants. Root length colonized by AMF, petiole nitrogen concentration, plant biomass and root morphology were evaluated. The  AMF community present in the grapevine roots growing in non-inoculated (control) and inoculated soil were profiled using high-throughput sequencing of 18S and ITS2 rRNA gene regions.

Results – The proportion of roots colonized with AMF fungal structures significantly increased for plants in inoculated soil, but the degree of colonization differed among commercial bioinoculants. Petiole nitrogen concentration increased and carbon to nitrogen ratio decreased for plants in inoculated soil. Shoot and root dry weight were increased for plants in inoculated soil when compared with plants in non-inoculated soil. Root diameter decreased and root length density and specific root length increased with greater AMF root colonization for plants in inoculated soil. The most predominant genus of AMF species from commercial bioinoculants colonizing roots in the inoculated soil was Rhizophagus, Glomus and Funneliformis, while Diversispora, Paraglomus, and Mortierella showed a low relative abundance respectively. A high relative abundance of the genus Glomus and Rhizophagus were found in roots of plants growing in the non-inoculated soil controls while Funneliformis, Paraglomus and Mortierella  were less predominant. Interestingly the  endophytic fungus Mortierella from the order Mortierellales is considered as a beneficial root colonizing fungus. These results suggest that the native AMF community of non-inoculated soil controls had limited effect on grapevine adaptative traits while inoculated soil with commercial bioinoculants containing specific species from the genus Glomus and Rhizophagus can modify root traits under soil nutrient deficiency and may be considered an alternative to replace chemical fertilizers .


Publication date: June 21, 2023

Issue: GiESCO 2023

Type: Article



1School of Integrative Plant Science, Cornell University, Ithaca, New York, United States
2School of Integrative Plant Science, Plant Pathology & Plant-Microbe Biology, Cornell University, Ithaca, New York, United States

Contact the author*


grapevine, bioinoculants, nitrogen, biomass, root traits, meta-barcoding


GiESCO | GIESCO 2023 | IVES Conference Series


Related articles…

Effect of foliar application of Ca, Si and their combination on grape volatile composition

Calcium (Ca) is an important nutrient for plants which plays key signaling and structural roles. It has been observed that exogenous Ca application favors the pectin accumulation and inhibition of polygalacturonase enzymes, minimizing fruit spoilage. Silicon (Si) is a non-essential element which has been found to be beneficial for improving crop yield and quality, as well as plant tolerance to diverse abiotic and biotic stress factors. The effect of Si supply to grapevine has been assessed in few investigations, which reported positive changes in grape quality and must composition.

Late winter pruning induces a maturity delay under temperature-increased conditions in cv. Merlot from Chile

Chile is considered vulnerable to climate change; and these phenomena affect several mechanisms in the grape physiology and quality. The global temperature increase affects sugar contents, organic acids, and phenolic compounds in grapes, producing an imbalance maturity. In this sense, an alternative to reduce the impact is to perform pruning after vine budburst, known as “Late Pruning” (LP).

Effect of two water deficit regimes on the agronomic response of 12 grapevine varieties cultivated in a semi-arid climate

The Mediterranean basin is one of the most vulnerable regions to Climate Change effects. According to unanimous forecasts, the vineyards of Castilla-La Mancha will be among the most adversely affected by rising temperatures and water scarcity during the vine’s vegetative period. One potential strategy to mitigate the negative impacts of these changes involves the identification of grapevine varieties with superior water use efficiency, while ensuring satisfactory yields and grape quality.

Influence of irrigation frequency on berry phenolic composition of red grape varieties cultivated in four spanish wine-growing regions

The global warming phenomenon involves the frequency of extreme meteorological events accompanied by a change in rainfall distribution. Irrigation frequency (IF) affects the spatial and temporal soil water distribution but its effects on the phenolic composition of the grape have been scarcely studied. The aim of this work was to evaluate the effects of four deficit irrigation frequencies of 30 % ETo: one irrigation per day (T01), two irrigations per week (T03), one irrigation per week (T07) and one irrigation every two weeks (T15) on berry phenolic composition at harvest.

Response of red grape varieties irrigated during the summer to water availability at the end of winter in four Spanish wine-growing regions: berry phenolic composition

Water availability is the most limiting factor for vineyard productivity under Mediterranean conditions. Due to the effects caused by the current climate change, wine-growing regions may face serious soil moisture conservation problems, due to the lower water retention capacity of the soil and higher soil irradiation. The aim of this work was to evaluate the effects of soil recharge irrigation in pre-sprouting and summer irrigation every week (30 % ETo) from the pea size state until the end of ripening (RP) compared to exclusively summer irrigation every week (R) in the same way that RP, on berry phenolic composition at harvest.