terclim by ICS banner
IVES 9 IVES Conference Series 9 GiESCO 9 Managing precision irrigation in vineyards: hydraulic and molecular signaling in eight grapevine varieties

Managing precision irrigation in vineyards: hydraulic and molecular signaling in eight grapevine varieties


Context and purpose of the study – Understanding the physiological and molecular bases of grapevine responses to mild to moderate water deficits is fundamental to optimize vineyard irrigation management and identify the most suitable varieties. In Mediterranean regions, the higher frequency of heat waves and droughts highlights the importance of precision irrigation to meet vine water demands and demonstrates the necessity for a deeper understanding of the different physiological responses among varieties under water stress. In this context, previous reports show an interplay between stomatal regulation of transpiration and changes in leaf hydraulic conductivity, also with the involvement of aquaporins (AQPs), particularly under water stress. However, how those signaling mechanisms are regulated in different grapevine varieties along phenological phases is unclear. We aimed to assess the impact of vine water status and phenology on stomatal and hydraulic adjustments, along with aquaporins expression, of leaves of 8 grapevine varieties (Petit Verdot (PV), Alicante Bouschet (AB), Syrah (S), Vinhão (V), Touriga Nacional (TN), Castelão (Cs), Trincadeira (TR) and Tinta Caiada (TC)) selected according to their contrasting carbon isotopic signatures.

Material and methods – Ten-year-old vines grafted on 1103P, grown in the Alentejo region (Portugal) as part of the ampelographic collection of Esporão commercial vineyard, were subjected to three different irrigation treatments since 2018: full irrigation (FI); deficit irrigation (DI, 50% of FI); no-irrigation (NI). Plant monitoring was done during 2021 at four phenological stages: pea-size (PS), veraison (VER), full-maturation (FM) and post-harvest (Post-H). Measurements included stomatal conductance (gs), predawn leaf water potential (ψpd), hydraulic conductivity (Kleaf) and the expression of 10 genes coding AQPs, previously reported to transport water in vitro.

Results – Results show that Kleaf is a function of grapevine phenology and variety. In all varieties and treatments, Kleaf peaked at veraison and declined thereafter (circa 80%). Water stress either increased or decreased Kleaf, likely depending on the ani- (e.g. TN) or isohydric (e.g. V, S) behavior. Interestingly, post-harvest irrigation increased Kleaf, surpassing values observed at FM, except in TN, suggesting that varieties benefit differently from late season irrigation. Stomatal conductance was highest at VER, in all irrigation treatments, except for TN, which peaked at PS. PV maintained constant gs throughout the season in all treatments. Non-irrigated AB, S, V, and TR maintained low and constant gs along the season. A significant correlation between gs and ψpd was observed, except in Cs and PV, indicating that in those varieties additional factors contribute to control stomatal aperture. Studied AQPs were all expressed at PS and VER in all varieties and water treatments, except in TC, TR and AB where some PIPs and NIPs were not expressed under water stress. VviPIP1;2 appears to be constitutive since it is expressed at all phenological stages, irrigation treatments and varieties. Overall, DI and NI induced an up-regulation of AQP expression in anisohydric varieties at PS and VER. In contrast, AQPs were down-regulated by stress at PS in the isohydric varieties. These results indicate that AQPs expression trend is variety and phenology dependent.


Publication date: July 5, 2023

Issue: GiESCO 2023

Type: Poster


Miguel DAMÁSIO1, Clara PINTO1,2, Teresa SOARES-DAVID1,2, Luísa CARVALHO3*, José SILVESTRE1*, Olfa ZARROUK3,4*

1INIAV – Instituto Nacional de Investigação Agrária e Veterinária, I.P., Oeiras, Portugal
2CEF – Centro de Estudos Florestais, Associated Laboratory TERRA, ISA-ULisboa, Lisboa, Portugal
3LEAF—Linking Landscape, Environment, Agriculture and Food Research Center, Associated Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal
4SFCoLAB – Laboratório Colaborativo para a Inovação Digital na Agricultura, Torres Vedras, Portugal

Contact the author*


aquaporins, hydraulic conductivity, stomatal conductance, water deficit


GiESCO | GIESCO 2023 | IVES Conference Series


Related articles…

Effect of foliar application of Ca, Si and their combination on grape volatile composition

Calcium (Ca) is an important nutrient for plants which plays key signaling and structural roles. It has been observed that exogenous Ca application favors the pectin accumulation and inhibition of polygalacturonase enzymes, minimizing fruit spoilage. Silicon (Si) is a non-essential element which has been found to be beneficial for improving crop yield and quality, as well as plant tolerance to diverse abiotic and biotic stress factors. The effect of Si supply to grapevine has been assessed in few investigations, which reported positive changes in grape quality and must composition.

Late winter pruning induces a maturity delay under temperature-increased conditions in cv. Merlot from Chile

Chile is considered vulnerable to climate change; and these phenomena affect several mechanisms in the grape physiology and quality. The global temperature increase affects sugar contents, organic acids, and phenolic compounds in grapes, producing an imbalance maturity. In this sense, an alternative to reduce the impact is to perform pruning after vine budburst, known as “Late Pruning” (LP).

Effect of two water deficit regimes on the agronomic response of 12 grapevine varieties cultivated in a semi-arid climate

The Mediterranean basin is one of the most vulnerable regions to Climate Change effects. According to unanimous forecasts, the vineyards of Castilla-La Mancha will be among the most adversely affected by rising temperatures and water scarcity during the vine’s vegetative period. One potential strategy to mitigate the negative impacts of these changes involves the identification of grapevine varieties with superior water use efficiency, while ensuring satisfactory yields and grape quality.

Influence of irrigation frequency on berry phenolic composition of red grape varieties cultivated in four spanish wine-growing regions

The global warming phenomenon involves the frequency of extreme meteorological events accompanied by a change in rainfall distribution. Irrigation frequency (IF) affects the spatial and temporal soil water distribution but its effects on the phenolic composition of the grape have been scarcely studied. The aim of this work was to evaluate the effects of four deficit irrigation frequencies of 30 % ETo: one irrigation per day (T01), two irrigations per week (T03), one irrigation per week (T07) and one irrigation every two weeks (T15) on berry phenolic composition at harvest.

Response of red grape varieties irrigated during the summer to water availability at the end of winter in four Spanish wine-growing regions: berry phenolic composition

Water availability is the most limiting factor for vineyard productivity under Mediterranean conditions. Due to the effects caused by the current climate change, wine-growing regions may face serious soil moisture conservation problems, due to the lower water retention capacity of the soil and higher soil irradiation. The aim of this work was to evaluate the effects of soil recharge irrigation in pre-sprouting and summer irrigation every week (30 % ETo) from the pea size state until the end of ripening (RP) compared to exclusively summer irrigation every week (R) in the same way that RP, on berry phenolic composition at harvest.