terclim by ICS banner
IVES 9 IVES Conference Series 9 GiESCO 9 GiESCO 2023 9 Hyperspectral imaging for the appraisal of varietal aroma composition along maturation in intact Vitis vinifera L. Tempranillo Blanco berries

Hyperspectral imaging for the appraisal of varietal aroma composition along maturation in intact Vitis vinifera L. Tempranillo Blanco berries


Context and purpose of the study – The knowledge of the grape aromatic composition during ripening provides very important information for winegrowers, who may carry out different viticultural practices, or determine the harvest date more accurately. However, there are currently no tools that allow this measurement to be carried out in a non-invasive and rapid way.  For this reason, the aim of this work was to design a non-invasive methodology, based on hyperspectral imaging to estimate the aromatic composition and total soluble solids (TSS) of Tempranillo Blanco berries during ripening. 

Material and methods – A total of 236 spectra of intact grape berries were acquired, under laboratory conditions, by hyperspectral imaging (HSI) in the visible + short wave near infrared (VIS+SW-NIR) range (400-1000 nm) to estimate the aromatic composition, and the TSS, of Vitis vinifera L. Tempranillo Blanco berries during ripening. Calibration, cross-validation and prediction models were built by partial least squares (PLS), using as reference the concentration of 20 grape berries’ volatile compounds (terpenoids, C13norisoprenoids, benzenoids, fatty acids, and C6 compounds), measured by gas chromatography – mass spectrometry (GC-MS), and the concentration of total soluble solids (TSS), measured by refractometry. 

Results – Values of determination coefficients of cross-validation (Rcv2) ≥ 0.70, were obtained for all terpenoids (α-terpineol citral, linalool, and p-cymene), all C13 norisoprenoids (β-damascenone and β-ionone) and their total, all benzenoids (benzaldehyde, 2-phenylethanol, and benzyl alcohol) and their total, two fatty acids (octanoic acid, and nonanoic acid), four C6 compounds (2-hexenal, hexanal, 2-hexen-1-ol, and (Z)-3-hexen-1-ol) and their total, the sum of all families except C6 compounds (called as total positive compounds), and TSS.  Therefore, it can be affirmed that hyperspectral imaging in the VIS+SW-NIR range could be a suitable tool to estimate the aromatic and industrial maturities of Tempranillo Blanco grape berries in a simultaneous, contactless and non-destructive way.


Publication date: July 3, 2023

Issue: GiESCO 2023

Type: Poster


S. Marín-San Román1, J. Fernández-Novales2,3*, C. Cebrián-Tarancón4, R. Sánchez-Gómez4, M. P. Diago2,3, T. Garde-Cerdán1

1Grupo VIENAP, Instituto de Ciencias de la Vid y del Vino (CSIC, Universidad de La Rioja, Gobierno de La Rioja). Ctra. de Burgos, Km. 6. 26007 Logroño, Spain
2Grupo TELEVITIS, Instituto de Ciencias de la Vid y del Vino (Universidad de La Rioja, CSIC, Gobierno de La Rioja). Ctra. de Burgos, Km. 6. 26007 Logroño, Spain
3Department of Agriculture and Food Science. University of La Rioja. Madre de Dios 53. 26007. Logroño, Spain
4Cátedra de Química Agrícola, E.T.S.I. Agrónomos y Montes, Departamento de Ciencia y Tecnología Agroforestal y Genética, Universidad de Castilla-La Mancha. Avda. de España, s/n. 02071 Albacete, Spain

Contact the author*


volatile compounds, partial least squares, total soluble solids, non-invasive, VIS+SW-NIR, TF-SPME


GiESCO | GIESCO 2023 | IVES Conference Series


Related articles…

Effect of foliar application of Ca, Si and their combination on grape volatile composition

Calcium (Ca) is an important nutrient for plants which plays key signaling and structural roles. It has been observed that exogenous Ca application favors the pectin accumulation and inhibition of polygalacturonase enzymes, minimizing fruit spoilage. Silicon (Si) is a non-essential element which has been found to be beneficial for improving crop yield and quality, as well as plant tolerance to diverse abiotic and biotic stress factors. The effect of Si supply to grapevine has been assessed in few investigations, which reported positive changes in grape quality and must composition.

Late winter pruning induces a maturity delay under temperature-increased conditions in cv. Merlot from Chile

Chile is considered vulnerable to climate change; and these phenomena affect several mechanisms in the grape physiology and quality. The global temperature increase affects sugar contents, organic acids, and phenolic compounds in grapes, producing an imbalance maturity. In this sense, an alternative to reduce the impact is to perform pruning after vine budburst, known as “Late Pruning” (LP).

Effect of two water deficit regimes on the agronomic response of 12 grapevine varieties cultivated in a semi-arid climate

The Mediterranean basin is one of the most vulnerable regions to Climate Change effects. According to unanimous forecasts, the vineyards of Castilla-La Mancha will be among the most adversely affected by rising temperatures and water scarcity during the vine’s vegetative period. One potential strategy to mitigate the negative impacts of these changes involves the identification of grapevine varieties with superior water use efficiency, while ensuring satisfactory yields and grape quality.

Influence of irrigation frequency on berry phenolic composition of red grape varieties cultivated in four spanish wine-growing regions

The global warming phenomenon involves the frequency of extreme meteorological events accompanied by a change in rainfall distribution. Irrigation frequency (IF) affects the spatial and temporal soil water distribution but its effects on the phenolic composition of the grape have been scarcely studied. The aim of this work was to evaluate the effects of four deficit irrigation frequencies of 30 % ETo: one irrigation per day (T01), two irrigations per week (T03), one irrigation per week (T07) and one irrigation every two weeks (T15) on berry phenolic composition at harvest.

Response of red grape varieties irrigated during the summer to water availability at the end of winter in four Spanish wine-growing regions: berry phenolic composition

Water availability is the most limiting factor for vineyard productivity under Mediterranean conditions. Due to the effects caused by the current climate change, wine-growing regions may face serious soil moisture conservation problems, due to the lower water retention capacity of the soil and higher soil irradiation. The aim of this work was to evaluate the effects of soil recharge irrigation in pre-sprouting and summer irrigation every week (30 % ETo) from the pea size state until the end of ripening (RP) compared to exclusively summer irrigation every week (R) in the same way that RP, on berry phenolic composition at harvest.