terclim by ICS banner
IVES 9 IVES Conference Series 9 GiESCO 9 Techniques to study graft union formation in grapevine 

Techniques to study graft union formation in grapevine 

Abstract

Context and purpose of the study – Grapevines are grown grafting in most viticultural regions. Grapevine rootstocks are either hybrids or pure species of different American Vitis spp. (particularly V. berlandieri, V. rupestris and V. riparia), which are primarily used to provide root resistance to the insect pest Phylloxera. In addition to Phylloxera resistance, ideally grapevine rootstocks should be resistant to other soil borne pathogens and adapted to abiotic stress conditions. New rootstocks have the potential to adapt agriculture to climate change without changing the characteristics of the harvested product. However, high grafting success rates are an essential prerequisite. The objective of this work is to develop quantitative techniques to characterize graft union formation in grapevine.

Material and methods – The development of grafts of different scion/rootstocks of grapevine was studied during the first few months after grafting. The quantity of callus produced (fresh and dry mass) and the mechanical strength of the graft union were quantified. We also used x-ray tomography to study functional xylem vessels by labelling functional vessels with the contrast agent Iohexol.

Results –  Equipment to quantify the mechanical strength of the graft union was assembled and tested on different scion/rootstock combinations to determine the suitability of this technique to quantify graft union development. The quantity of callus produced at the graft interface different between different genotypes and was not necessarily related to the mechanical strength of the graft union. Three dimensional reconstruction of x-ray tomography images allowed us to visualize the connections between the scion and rootstock, and this knowledge will be used to develop protocols to quantify xylem vessel connections using simpler methods. 

Significance of the study – Difficulties in quantatively phenotyping the different steps of graft union formation have considerably retarded the identification of the genetic determinants of grafting success in all plant species. We are developing different quantative techniques to overcome this bottleneck with the view to charactering the genetic architecture of graft union formation in grapevine.

DOI:

Publication date: July 7, 2023

Issue: GiESCO 2023

Type: Poster

Authors

M. Camboué1, G. Loupit1, A. Janoueix1, J.-P. Tandonnet1, M. Morel1, E. Marguerit1, F. Cordelières3, J. Teillon3, G. Mathieu2, A.-S. Splimont2, S. J. Cookson1*

1EGFV, Univ. Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV, F-33882, Villenave d’Ornon, France
2Institut Français de la Vigne et du Vin, Domaine de l’Espiguette, Le Grau-du-Roi, France
3Univ. Bordeaux, CNRS, INSERM, Bordeaux Imaging Center, BIC, UMS 3420, US 4, F-33000 Bordeaux, France

Contact the author*

Keywords

grapevine, xylem vessels, grafting, callus, mechanical strength

Tags

GiESCO | GIESCO 2023 | IVES Conference Series

Citation

Related articles…

Physiological and growth reaction of Shiraz/101-14 Mgt to row orientation and soil water status

Advanced knowledge on grapevine row orientation is required to improve establishment, management and outcomes of vineyards on terroirs with different environmental conditions (climate, soil, topography) and in view of a future change to more extreme climatic conditions. The purpose of this study was to determine the combined effect of row orientation, plant water status and ripeness level on the physiological and viticultural reaction of Shiraz/101-14 Mgt.

Effects of mechanical leafing and deficit irrigation on Cabernet Sauvignon grown in warm climate of California

San Joaquin Valley accounts for 40% of wine grape acreage and produces 70% of wine grape in California. Fruit quality is one of most important factors which impact the economical sustainability of farming wine grapes in this region. Due to the recent drought and expected labor cost increase, the wine industry is thrilled to understand how to improve fruit quality while maintaining the yield with less water and labor input. The present study aims to study the interactive effects of mechanical leafing and deficit irrigation on yield and berry compositions of Cabernet Sauvignon grown in warm climate of California.

The effects of cane girdling on berry texture properties and the concentration of some aroma compounds in three table grape cultivars

The marketability of the table grapes is highly influenced by the consumer demand; therefore the market value of the table grapes is mainly characterized by its berry size, colour, taste and texture. Girdling could cause accumulation of several components in plants above the ringing of the phloem including clusters and resulting improved maturity. The aim of the experiments was to examine the effect of girdling on berry texture characteristics and aroma concentration.

Application of a fluorescence-based method to evaluate the ripening process and quality of Pinot Blanc grape

The chemical composition of grape berries at harvest is one of the most important factors that should be considered to produce high quality wines. Among the different chemical classes which characterize the grape juice, the polyphenolic compound, such as flavonoids, contribute to the final taste and color of wines. Recently, an innovative non-destructive method, based on chlorophyll fluorescence, was developed to estimate the phenolic maturity of red grape varieties through the evaluation of anthocyanins accumulated in the berry skin. To date, only few data are available about the application of this method on white grape varieties.

Different yield regulation strategies in semi-minimal-pruned hedge (SMPH) and impact on bunch architecture

Yields in the novel viticulture training system Semi-Minimal-Pruned Hedge (SMPH) are generally higher compared to the traditional Vertical Shoot Positioning (VSP). Excessive yields have a negative impact on the vine and wine quality, which can result in substantial losses in yield in subsequent vintages (alternate bearing) or penalties in fruit quality. Therefore yield regulation is essential. The bunch architecture in SMPH differs from VSP. Generally there is a higher amount but smaller bunches with lower single berry weights in SMPH compared to VSP.