OENO IVAS 2019 banner
IVES 9 IVES Conference Series 9 Grape seed powder as an alternative to bentonite for wine fining

Grape seed powder as an alternative to bentonite for wine fining

Abstract

PR proteins can cause haze in wines, and the risk is to keep the wine unsold. Generally, in winemaking bentonite solves this problem by removing proteins, but it is not a renewable resource, has poor settling, which means difficulty in filtering after use and a considerable loss of wine, it is not a specific adsorbent and may reduce aromas and flavor compounds. This work studied the use of grape seeds powder (GSP) to remove haze-forming proteins from wine and grape juice. GSP was tried both roasted 180°C x 10 minutes and unroasted, while contact time was set at one hour and two hours for comparison. GSP was tried first on four different heat-unstable wines in small-scale experiments. The results showed that GSP removed PR proteins and permitted to achieve heat stability (DNTU<2) but with high doses (25-32 g/L) of addition. A similar reduction of PR proteins was obtained in all the wines after 1-h contact time with unroasted GSP as wells as with roasted GSP, which suggests that roasting did not substantially alter the protein-binding capability of GSP. Contact time (1 or 2 hours) did not change the efficacy of protein removal suggesting that the reaction between grape tannins and proteins occurs within one hour. Treated wines showed changes in the matrix composition, with increased phenolic contents (A280) and improved yellow color (CIELAB b* parameter). As for the experiments with grape juice, GSP was added in two juices before fermentation to observe the impact on the composition of the finished wines. Roasted GSP was chosen as the fining agent and the contact time was 1 hour. A lower amount of GSP (5 g/L) was observed to be needed to heat-stabilize (DNTU<2) the juices. The corresponding wines showed minor changes in the matrix composition, perhaps because of phenolic-protein interaction and precipitation during the fermentation or degradation via non-enzymatic processes. These results suggested that GSP may be a viable alternative to bentonite. Furthermore, being a by-product of winemaking, GSP utilization would improve the environmental sustainability of winemaking processes.

DOI:

Publication date: June 10, 2020

Issue: OENO IVAS 2019

Type: Article

Authors

Elia Romanini, Jacqui M. McRae, Donato Colangelo, Milena Lambri

The Australian Wine Research Institute, Waite Precinct, Hartley Grove cnr Paratoo Road, Urrbrae (Adelaide), PO Box 197, Glen Osmond, SA 5064, Australia.
UniversitàCattolica del Sacro Cuore – DiSTAS Via Emilia Parmense, 84, 29122 Piacenza, Italy.

Contact the author

Keywords

grape seeds, bentonite, fining, hazing proteins 

Tags

IVES Conference Series | OENO IVAS 2019

Citation

Related articles…

Berry weight loss in Vitis vinifera (L.) cultivars during ripening

Berry shriveling (BS) in vineyards are caused by numerous factors such as sunburn, dehydration, stem necrosis. Climate change results in an increase in day and night temperatures, rainfall throughout the year, changes in the timing and quantities, long dry summers and a combination of climatic variability such as floods, droughts and heatwaves). Grape development and its composition at harvest is influenced by the latter as grape metabolites are sensitive to the environmental conditions. The grape berry experiences water loss and an increase in flavour development as a result of the BS. An increased sugar content in grapes will result in higher alcohol wines and concentration of grape aromas which may be detrimental to the final wine quality.

Phenological stage dependency of Cabernet Sauvignon and Grenache response to water and nutrient limitation 

As the frequency and intensity of drought events increase, understanding the mechanisms of plant resilience to water deficit is crucial. To maintain an appropriate plant yield, a common practice is the application of high amounts of fertilizers with negative environmental impacts. The single and combined effect of water deficit and nutrient availability, namely nitrogen (N) and potassium (K), in Vitis Vinifera L. cv. Cabernet Sauvignon and Grenache was evaluated. Two-year-old grapevine plants grafted on SO4 rootstock were transferred in pots under semi-environmental conditions. During the growing season, plants were either maintained well-watered (100% ETc) or subjected to a controlled water deficit irrigation (33% ETc).

Effect of one-year cover crop and arbuscular mycorrhiza inocululation in the microbial soil community of a vineyard

The microbial composition of the soil is an important factor to consider in viticulture, since its influence on the “terroir” and on the organoleptic properties of the wine have been demonstrated. Different agronomic techniques have the potential to modify the composition and functionality of the soil microbial community. Maintaining green covers is known to increase soil microbial diversity. The direct application of inoculum of beneficial microorganisms to the soil has also been used to increase their abundance. However, the environmental conditions of each site seem to have a determining weight in the result of these practices. In this study, we compared the effect on the microbial community of a cover crop with legumes in autumn and the inoculation of grapevines with commercial inoculum bases on Rhizophagus irregularis and Funeliformis mosseae in the previous spring. The study has been carried out in a vineyard in Binissalem, Mallorca, Spain. After applying the treatments, we will analyze the soil microbial communities using the data obtained from Illumina amplification of soil DNA from the 16S and ITS regions to analyze bacteria and fungi community, respectively. In addition, we will record the physicochemical characteristics of the soil at each sampling point. The result showed that agronomic management, in the short term, has less influence than soil characteristics on the composition of the soil microbiome. With these results, we can conclude that in a vineyard, agricultural techniques should focus on improving the characteristics of the soil to improve the biodiversity of the soil microbiota.

The French grapevine breeding program resdur: state of the art and perspectives

The French grapevine breeding program for durable resistance to downy and powdery mildew (INRAE-ResDur) was initiated more than 20 years ago to help reduce the heavy use of plant protection products and provide a durable mean to cope with a strong pathogen pressure. This program has now proved to be effective, with about ten new varieties already officially registered. However, there is still a lot to be done (1) to reduce the duration of each breeding cycle, (2) to diversify disease factors’ pyramiding and anticipate emerging diseases, (3) to work towards larger adoption of the new resistant varieties. New breeding schemes incorporating for example genomic prediction of breeding values are being evaluated to accelerate genetic gains, saving cost and time while handling complex traits.

Grape metabolites, aroma precursors and the complexities of wine flavour

A critical aspect of wine quality from a consumer perspective is the overall impression of wine flavour, which is formed by the interplay of volatile aroma compounds, their precursors, and taste and matrix components. Grapes contribute some potent aroma compounds, together with a large pool of non-volatile precursors (e.g. glycoconjugates and amino acid conjugates). Aroma precursors can break down through chemical hydrolysis reactions, or through the action of yeast or enzymes, significantly changing the aroma profile of a wine during winemaking and storage. In addition, glycoconjugates of monoterpenes, norisoprenoids and volatile phenols, together with sulfur-conjugates in wine, provide a reservoir of additional flavour through the in-mouth release of volatiles which may be perceived retro-nasally.