OENO IVAS 2019 banner
IVES 9 IVES Conference Series 9 OENO IVAS 9 OENO IVAS 2019 9 Chemical and Biochemical reactions, including grape and wines microorganisms impact 9 Impact of glutathione-rich inactivated yeast on wine chemical diversity

Impact of glutathione-rich inactivated yeast on wine chemical diversity

Abstract

Glutathione-rich inactivated dry yeasts (GSH-IDY) are claimed to accumulate intracellularly and then release glutathione in the must. Glutathione is beneficial to the wine quality, but scientists also highlighted that GSH-IDYs have a greater effect than only increase the pool of this antioxidant in the wine. This work unveils the extent of diversity of compounds potentially released by three different IDYs with increasing GSH contents.

Unsupervised analysis of IDYs released compounds in model wine was performed with the ultra-high-resolution Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR-MS). This powerful tool allows to have an instant picture of the released compounds chemical diversity. Bioinformatics strategy (chemometric analysis and network annotation) were then applied to visualize and refine the generated data.

Our results clearly show an impact of the GSH accumulation process not only visible on the glutathione itself, but also on the global diversity of compounds. The ratio of annotated CHONS/CHO ions increased from 0.2 to 2.1 respectively with the accumulation of GSH. The IDY with the highest concentration of GSH released 36 unique CHONS annotated ions compared to the two others IDYs. Since the bioprocess dedicated to accumulate the intracellular glutathione used cysteine rich medium, the possibility to attribute this diversity to notably a larger number of cysteinyl residues in peptides raised. Within the 1699 detected ions by (-)FT-ICR-MS, 193 were annotated as peptide sequences (from 2 to 5 residues). Within this pool of peptides, the IDY specific diversity increased with the level of glutathione from 5 to 45 unique m/z. Besides the global diversity, m/z attributed to cysteine containing peptides were much more abundant in the GSH-rich IDY. Within the 25 peptides containing cysteine, and common to the three IDYs, 64 % were more intense in GSH-rich IDY. Thus, the process leading to accumulate glutathione is also involved in other metabolic pathways which contribute to increase CHONS containing compounds and notably peptides.

This work gives new clues on the potential of biotechnology to improve the efficiency of natural yeast derivatives to produce potential active compounds such as cysteine containing peptides. This could lead to substitute partially the chemical additives and thus leading to a better control of wine quality and a better consumer acceptability.

DOI:

Publication date: June 11, 2020

Issue: OENO IVAS 2019

Type: Article

Authors

Florian Bahut, Youzhong Liu, Rémy Romanet, Nathalie Sieczkowski, Hervé Alexandre, Christian Coelho, Philippe Schmitt-Kopplin, Maria Nikolantonaki, Régis D. Gougeon

Lallemand SAS, 19 rue des Briquetiers, 31702 Blagnac, France
Research Unit Analytical BioGeoChemistry, Helmholtz Zentrum Muenchen, 85764 Neuherberg, Germany
Technische Universität München, Analytical Food Chemistry, Akademie 10, 85354 Freising, Germany

Contact the author

Email address (with mailto: link)

Keywords

yeast derivative, glutathione enrichment, metabolomic, peptide diversity 

Tags

IVES Conference Series | OENO IVAS 2019

Citation

Related articles…

Chemical boundaries of wine identity: rationalizing grape and wine aroma diversity for improved terroir management

Aims: Wine perceived quality lies on a number of different factors. Among these, sensory features, which are in turn dependent on chemical composition, play a primary role. There is traditionally a great emphasis on producing wines that have specific sensory profiles, particularly aroma, that reflect identity features connected to the place and the variety of origin. In the case of high quality

Rationalising the impact of time, light, temperature, and oxygen on the evolution of rosé wines by means of a surface response methodology approach

The widespread use of flint glass bottles for rosé wines is driven by consumer preference for color as a key choice factor.

Critical investigation on additions to improve the sensory characteristics of dealcoholized wine

The demand for dealcoholized wine has been progressively increasing in recent years. Moreover, the attention for such products is probably increasing even more. Due to that increasing demand and market awareness the legal authorities are about changing rules for that products. Also, at OIV level, these products are being intensively discussed for certain time. The production of dealcoholized wine bases on wine as initial product. This wine is then reduced by physical methods to an alcohol content of less than 0.5% vol., or in other words, to less than 4g/l of alcohol. There are various technologies are possible for producing dealcoholized wine (Schmitt and Christmann 2019).

Grapevine yield estimation in a context of climate change: the GraY model

Grapevine yield is a key indicator to assess the impacts of climate change and the relevance of adaptation strategies in a vineyard landscape. At this scale, a yield model should use a number of parameters and input data in relation to the information available and be able to reproduce vineyard management decisions (e.g. soil and canopy management, irrigation). In this study, we used data from six experimental sites in Southern France (cv. Syrah) to calibrate a model of grapevine yield limited by water constraint (GraY). Each yield component (bud fertility, number of berries per bunch, berry weight) was calculated as a function of the soil water availability simulated by the WaLIS water balance model at critical phenological phases. The model was then evaluated in 10 grapegrowers’ plots, covering a diversity of biophysical and technical contexts (soil type, canopy size, irrigation, cover crop). We identified three critical periods for yield formation: after flowering on the previous year for the number of bunches and berries, around pre-veraison and post-veraison of the same year for mean berry weight. Yields were simulated with a model efficiency (EF) of 0.62 (NRMSE = 0.28). Bud fertility and number of berries per bunch were more accurately simulated (EF = 0.90 and 0.77, NRMSE = 0.06 and 0.10, respectively) than berry weight (EF = -0.31, NRMSE = 0.17). Model efficiency on the on-farm plots reached 0.71 (NRMSE = 0.37) simulating yields from 1 to 8 kg/plant. The GraY model is an original model estimating grapevine yield evolution on the basis of water availability under future climatic conditions.  It allows to evaluate the effects of various adaptation levers such as planting density, cover crop management, fruit/leaf ratio, shading and irrigation, in various production contexts.

Unveiling the bioactive potential of aglianco grape pomace: oleanolic acid as a promising natural product

The winemaking industry generates a substantial amount of byproducts, including grape pomace, which is often discarded as waste. However, this seemingly useless material holds a wealth of bioactive compounds with potential health benefits. Recognizing the value of circular economy principles, this study delves into the comprehensive chemical analysis of aglianco grape pomace, aiming to transform this byproduct into a valuable resource.