NMR approach for monitoring the photo-degradation of riboflavin and methionine

Landscapes such as its representation express values, beliefs and intentions of the individuals and the communities that produce them.

Stilbenes are natural bioactive polyphenols produced by grapevine. Recently, we have reviewed the na- tural presence of these compounds in wines [1]. This study showed that the resveratrol and its glycoside, the piceid, are the most abundant stilbenes in wines. Resveratrol is a well-known stilbene with a wide range of biological activities. Due to its specific structure, resveratrol can be oxidized in wines to form various derivatives including oligomers [2]. In this study, we investigate the resveratrol and piceid transformation in wines.

Sulfites (SO2) addition during winemaking is a widespread practice worldwide. This addition is realized at different steps of the winemaking due to the antimicrobial and antioxidant capacity of SO2. In a context of understanding white wines oxidative stability, knowledge about the impact of SO2 on the wine molecular diversity, especially compounds involved in the antioxidant capacity of wine, appears to be very important. In recent years, some studies have shown that SO2 can react with a large number of wine compounds resulting in the formation of numerous adducts. The diversity of compounds involved is important including in particular pyruvic acid, 2-keto-glutaric acid, glyceraldehyde, sugar, phenolics compounds but also amino acids or peptides. Moreover Roullier-Gall et al. have shown using FT-ICR-MS analysis that the molecular composition of wines remains impacted by addition of SO2 to the must (0, 4 and 8 g/hL SO2), several years after winemaking. Indeed, wines made from protected must (8g/hL SO2) contain a larger diversity of CHOS and CHONS compounds than wines made from unprotected must (0 g/hL SO2). The study of the impact of glutathione addition on the sensory oxidative stability has further shown that CHOS and CHONS compounds (amino acids, aromatic compounds and peptides) are markers of the antioxidant metabolome of white wines. This suggests that CHOS and CHONS compounds arise from SO2 adducts formation but also from a protecting effect of SO2 on the antioxidant metabolome of white wines.

Vigor declines in older vineyards and poor vine establishment in replant situations have been attributed to plant-parasitic nematodes. The northern root-knot nematode, Meloidogyne hapla, is the most prevalent plant-parasitic nematode species found in Washington wine grape vineyards. Management for nematodes in established vineyards is limited to the application of post-plant nematicides. We are evaluating new nematicides that are currently not registered in grape for their efficacy in controlling M. hapla and a part of that evaluation includes improving the alignment of nematicide application timing with the vulnerable second-stage juvenile (J2) life stage of M. hapla.

AIM: Biofilm is a resistance mechanism deployed by microorganisms to adapt to stresses, leading to their persistence in the environment. In the case of Brettanomyces bruxellensis, a wine spoilage yeast, knowledge about its capacity to form biofilm remains limited although this potential strategy could explain its recurring presence in cellars.