terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Characterization of spoilage yeasts from Malbec grapes from San Rafael wine region (Argentina)

Characterization of spoilage yeasts from Malbec grapes from San Rafael wine region (Argentina)

Abstract

The yeast ecosystem in grape musts is quite broad and depends on the region and the health of the grapes. Within this, there are yeasts that can generate fermentative deviations and/or cause defects in the wine. It is very important to address this issue because there are significant economic losses in the wine industry when the fermentation process and/or the organoleptic characteristics of the wine are negatively affected, even more today since climate change has a marked effect on the composition of this ecosystem. The aim of this work is to characterize the behavior regarding detrimental oenological features of potential spoilage yeasts isolated from viticultural environments. Therefore, 36 non-Saccharomyces yeasts belonging to 9 species previously isolated from wine grapes cv. Malbec from 4 districts of the winemaking region DO San Rafael (Mendoza, Argentina) were evaluated for their ability to produce acetic acid in calcium carbonate medium, hydrogen sulfide (H2S) in BIGGY agar medium and undesirable aromas by a sensory evaluation after incubation in pasteurized grape juice with p-coumaric acid. In general, Pichia kudriavzevii strains were the larger producers of aroma defects, different from “Brett”, and H2S, but there was no sign of acid production. Strains of Hanseniaspora uvarum and Hanseniaspora guilliermondii produced unpleasant aromas, high acetic acid and low values of H2S production. Other strains that showed interesting results were Zygosaccharomyces bailii and Hanseniaspora vineae; they were H2S producers and acidogenic, however they did not produce significant defects in the grape must screening test. There are some strains that could be consider as possible wine spoilage yeasts but deeper studies need to be done. It is important to study them in order to know the main species responsible for the more significant damage in oenology with the purpose of controlling them.

DOI:

Publication date: October 10, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Juliana Garau1, María Gabriela Merín1, María Carolina Martín1, Eugenia Sevillano1, Vilma Inés Morata de Ambrosini1

1Instituto de Ciencias Aplicadas a la Industria- ICAI (Consejo Nacional de Investigaciones Científicas y Técnicas -CONICET/ Facultad de Ciencias Aplicadas a la Industria, Universidad Nacional de Cuyo). Bernardo De Irigoyen 375, CP: 5600, San Rafael (Argentina).

Contact the author*

Keywords

spoilage yeasts, San Rafael wine region, Malbec, aroma defects, H2S, acidogenic

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Climate change and viticulture in Nordic Countries and the Helsinki area

The first vineyards in Northern Europe were in Denmark in the 15th century, in the southern parts of Sweden and Finland in the 18th century at 55–60 degrees latitude. The grapes grown there have not been made into wine, but the grapes have been eaten at festive tables. The resurgence of viticulture has started with global warming, and currently the total area of viticulture in the Nordic countries, including Norway, is estimated to be 400–500 hectares, most of which is in Denmark. Southern Finland, like all southern parts of Northern Europe, belongs to the cool-cold winegrowing area.

Metabolomic profiling of botrytized grape berries: unravelling the dynamic chemical transformations during noble rot

Botrytis cinerea, a fungal pathogen commonly known as grey mold, which under specific climatic conditions can develop into a desirable form known as noble rot. In this process the fungus penetrates the grape skin, allowing water evaporation and concentration of sugars and flavors, while profoundly affects the metabolite composition of grapes, leading to the production of unique and desirable compounds in the resulting wines. The result is a unique and complex wine with a luscious sweetness, heightened aromatics, and a distinct character.

Effect of biological control agents on grapevine rhizosphere microbiome and grapevine defenses

Plant diseases are a major obstacle to crop production. The main approaches to battle plant diseases, consist of synthetic chemicals to attack infecting pathogens. However, concerns are increasing about the effects of chemicals in the environment, leading to an increase in the use of biocontrol agents (BCAs), due to their assets, such as, antagonism, and competition. In this study, we tested the hypothesis that the introduction of Bacillus subtilis PTA-271 (Bs PTA-271) and Trichoderma atroviride SC1 (Ta SC1) produce distinctive modifications in the composition and network structure of the grapevine rhizosphere microbial community, as well as grapevine induced defenses.

Development and validation of a free solvent UHPLC/MS-MS method to analyse melatonin and its precursors in Spanish commercial wines  

Melatonin is a bioactive compound present in foods and beverages such as wines. During alcoholic fermentation, yeast transforms tryptophan into certain indole compounds, including melatonin. This paper aims to develop and validate a free solvent analytical method by ultra-high performance liquid chromatography coupled with high resolution mass spectrometry (UHPLC/MS-MS) to determine melatonin and its precursors (L-tryptophan, tryptamine, serotonin, tryptophol, N-acetylserotonin, 5-hydroxytryptophan, and 3- indoleacetic) that appropriately prevent the matrix effect.

Role of anthocyanins and copigmentation in flavonol solubility in red wines 

Over the last years, due to climate change, several red wines, such as the Sangiovese wines, have been often subjected to loss of clarity due to the formation of deposits of fine needle-shaped crystals. This phenomenon turned out to be due to an excess of quercetin (Q) and its glycosides (Q-Gs) in wines. These compounds are synthesized to a large extent when grapes are excessively exposed to UVB radiations in vineyards[1]. Unfortunately, it is not easy to predict the degree of Q precipitation because its solubility strongly depends on the wine and matrix composition[2].