terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Characterization of spoilage yeasts from Malbec grapes from San Rafael wine region (Argentina)

Characterization of spoilage yeasts from Malbec grapes from San Rafael wine region (Argentina)

Abstract

The yeast ecosystem in grape musts is quite broad and depends on the region and the health of the grapes. Within this, there are yeasts that can generate fermentative deviations and/or cause defects in the wine. It is very important to address this issue because there are significant economic losses in the wine industry when the fermentation process and/or the organoleptic characteristics of the wine are negatively affected, even more today since climate change has a marked effect on the composition of this ecosystem. The aim of this work is to characterize the behavior regarding detrimental oenological features of potential spoilage yeasts isolated from viticultural environments. Therefore, 36 non-Saccharomyces yeasts belonging to 9 species previously isolated from wine grapes cv. Malbec from 4 districts of the winemaking region DO San Rafael (Mendoza, Argentina) were evaluated for their ability to produce acetic acid in calcium carbonate medium, hydrogen sulfide (H2S) in BIGGY agar medium and undesirable aromas by a sensory evaluation after incubation in pasteurized grape juice with p-coumaric acid. In general, Pichia kudriavzevii strains were the larger producers of aroma defects, different from “Brett”, and H2S, but there was no sign of acid production. Strains of Hanseniaspora uvarum and Hanseniaspora guilliermondii produced unpleasant aromas, high acetic acid and low values of H2S production. Other strains that showed interesting results were Zygosaccharomyces bailii and Hanseniaspora vineae; they were H2S producers and acidogenic, however they did not produce significant defects in the grape must screening test. There are some strains that could be consider as possible wine spoilage yeasts but deeper studies need to be done. It is important to study them in order to know the main species responsible for the more significant damage in oenology with the purpose of controlling them.

DOI:

Publication date: October 10, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Juliana Garau1, María Gabriela Merín1, María Carolina Martín1, Eugenia Sevillano1, Vilma Inés Morata de Ambrosini1

1Instituto de Ciencias Aplicadas a la Industria- ICAI (Consejo Nacional de Investigaciones Científicas y Técnicas -CONICET/ Facultad de Ciencias Aplicadas a la Industria, Universidad Nacional de Cuyo). Bernardo De Irigoyen 375, CP: 5600, San Rafael (Argentina).

Contact the author*

Keywords

spoilage yeasts, San Rafael wine region, Malbec, aroma defects, H2S, acidogenic

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Impact of toasting and botanical origin on oak wood (Q. sp.) volatilome using untargeted GCxGC-ToFMS analysis

Many works have been carried out to identify the key aroma volatile compounds of oak wood (e.g., whisky-lactone, furfural, maltol, eugenol, guaiacol, vanillin) using conventional gas chromatography coupled with olfactometry and mass spectrometry (GC-O-MS). Inspired by recent untargeted approaches in the field of food “omics”, this work aims to extend our knowledge on the impact of cooperage process on the volatile composition of oak wood using two-dimensional comprehensive gas chromatography coupled with time of flight mass spectrometry (GCxGC-ToFMS).

Oenococcus oeni clonal diversity in the carbonic maceration winemaking

This essay was aimed to describe the clonal diversity of Oenococcus oeni in the malolactic fermentation of the carbonic maceration (CM) winemaking. The free and the pressed liquids from CM were sampled and compared to the wine from a standard winemaking with previous destemming and crushing (DC) of grapes [1]. O. oeni strain typification was performed by PFGE as González-Arenzana et al. described (2014) [2]. Results showed that 13 genotypes, referred as to letters, were distinguished from the 49 isolated strains, meaning the genotype “a” the 27%, the “b” the 14%, the “c” the 12%, the “d and e” the 10 % each other, and the remaining ones less than the 8% each one.

White grape must processed by UHPH as an alternative to SO2 addition: Effect on the phenolic composition in three varieties

The quantity and distribution of polyphenols in musts play a fundamental role in the white winemaking. This is because these substances are exposed to oxidation reactions, which are catalysed by the polyphenol oxidase (PPO), leading to a decrease in the quality of the wines produced. PPO is inactivated by SO2, but currently, due to the restrictions of the legislation, other methodologies are being investigated. Ultra-High Pressure Homogenization (UHPH) is a non-thermal physic technology that exerts an ultrahigh pressure pumping (>200 MPa) of a fluid through a valve in a continuous system.

Potential of new genetic resources to improve drought adaptation of grapevine rootstocks

Grapevines are grown mainly as grafts worldwide, but the rootstocks most commonly used were selected between the late 19th and early 20th centuries and are based on reduced genetic diversity[1]. In the context of climate change, it is indeed urgent to diversify the range of rootstocks with genotypes much more adapted to drier environments, than the existing ones[2]. The aim of this study was to evaluate the potential of new genetic resources for grapevine rootstock breeding programs. For this purpose, 12 American and Asian wild Vitis species (3 to 5 accessions per species = 50 accessions) were evaluated for their rooting ability and drought response.

Exploring the prevalence of esca-induced leaf symptoms in French vineyards and the role of climate: a national scale analysis

Esca, a severe trunk disease affecting vineyards, is caused by fungal pathogens that induce wood necrosis and decay, leaf symptoms, yield losses, and potentially a rapid death of the vine. The prevalence of this disease varies across years, regions, cultivars, and plot ages. Despite its significance in understanding and predicting dieback risk in different vineyards, the role of climate in trunk diseases remains a relatively unexplored research area. While some studies have demonstrated the impact of certain climatic conditions on the prevalence of the disease, they often focus on a limited number of plots and yield conflicting results.We conducted a statistical analysis, using a Bayesian approach on a national database comprising prevalence data of esca from over 500 different plots in France, spanning the years 2003 to 2022 and encompassing various cultivars.