terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Characterization of spoilage yeasts from Malbec grapes from San Rafael wine region (Argentina)

Characterization of spoilage yeasts from Malbec grapes from San Rafael wine region (Argentina)

Abstract

The yeast ecosystem in grape musts is quite broad and depends on the region and the health of the grapes. Within this, there are yeasts that can generate fermentative deviations and/or cause defects in the wine. It is very important to address this issue because there are significant economic losses in the wine industry when the fermentation process and/or the organoleptic characteristics of the wine are negatively affected, even more today since climate change has a marked effect on the composition of this ecosystem. The aim of this work is to characterize the behavior regarding detrimental oenological features of potential spoilage yeasts isolated from viticultural environments. Therefore, 36 non-Saccharomyces yeasts belonging to 9 species previously isolated from wine grapes cv. Malbec from 4 districts of the winemaking region DO San Rafael (Mendoza, Argentina) were evaluated for their ability to produce acetic acid in calcium carbonate medium, hydrogen sulfide (H2S) in BIGGY agar medium and undesirable aromas by a sensory evaluation after incubation in pasteurized grape juice with p-coumaric acid. In general, Pichia kudriavzevii strains were the larger producers of aroma defects, different from “Brett”, and H2S, but there was no sign of acid production. Strains of Hanseniaspora uvarum and Hanseniaspora guilliermondii produced unpleasant aromas, high acetic acid and low values of H2S production. Other strains that showed interesting results were Zygosaccharomyces bailii and Hanseniaspora vineae; they were H2S producers and acidogenic, however they did not produce significant defects in the grape must screening test. There are some strains that could be consider as possible wine spoilage yeasts but deeper studies need to be done. It is important to study them in order to know the main species responsible for the more significant damage in oenology with the purpose of controlling them.

DOI:

Publication date: October 10, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Juliana Garau1, María Gabriela Merín1, María Carolina Martín1, Eugenia Sevillano1, Vilma Inés Morata de Ambrosini1

1Instituto de Ciencias Aplicadas a la Industria- ICAI (Consejo Nacional de Investigaciones Científicas y Técnicas -CONICET/ Facultad de Ciencias Aplicadas a la Industria, Universidad Nacional de Cuyo). Bernardo De Irigoyen 375, CP: 5600, San Rafael (Argentina).

Contact the author*

Keywords

spoilage yeasts, San Rafael wine region, Malbec, aroma defects, H2S, acidogenic

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

How are canned wine drinkers perceived? An investigation involving Swiss nationals and different scenarios of outdoor leisure activities

This study examines how people who consume wine in cans are perceived in terms of their basic personality characteristics, helps understand the role of cultural background on people’s perception, and verify the role played by the consumption context on the perception. Our hypothesis is that prejudice and negative attitudes towards wine in cans might exert a negative effect on the evaluation of people who consume canned wine. To evaluate this hypothesis, the consumption of wine in cans was evoked in four different contexts of use during outdoor leisure activity (beach resort, ski resort, desert safari, and party). In order to examine the effect of culture on subject’s response we use participants from Switzerland, a country where three different cultures, associated with three different languages, cohabit.

A comprehensive study on the effect of foliar mineral treatments on grapevine microbiota, flavonoid gene expression, and berry composition

Recently, foliar treatments with mineral-based compounds have shown positive effects on grapevine production by protecting grape from thermal excesses and reducing the decoupling between technological and phenolic maturity caused by climate change. Unraveling the effect of mineral particle applications on grape-associated microbes is pivotal for successful wine processing, due to the influence of the microbiota on wine composition and stability. To our knowledge, this is the first work that comprehensively studied the effects of kaolin and chabasite-rich zeolitites treatments on grape-related microorganisms (by real-time PCR quantification of total fungi, Hanseniospora uvarum, Metschnikowia pulcherrima, plant-associated bacteria and lactic acid bacteria), the expression of genes related to the flavonoid biosynthesis (PAL1, CHS1, F3H2, DFR, LDOX, UFGT, MYBA1, GST4, FLS4 genes) and the berry composition (°Brix, pH, acidity and anthocyanin concentrations) in cv. Sangiovese during ripening in two growing seasons (2019 and 2020).

The exploitation of Croatian grapevine genetic resources for the breeding of new resistant cultivars 

Croatian viticulture is mainly based on native grapevine varieties susceptible to various diseases and pests, which leads to unsustainable use of large amounts of pesticides. The sustainable development of viticulture in the future will only be possible by increasing the resistance of the grapevine through the development of new resistant varieties. Breeding programs have been launched in the leading wine-growing countries to develop resistant varieties possessing high-quality levels. Native cultivars from Croatia are not included in the breeding programs of other countries.

Model-assisted analysis of the root traits underlying RSA genotypic diversity in Vitis: a promising approach for rootstock selection?

By dissecting the root system architecture (RSA) into its underpinning components (e.g. root emission, axial growth, radial growth, branching, root direction or tropism) and identifying the relationships between them, functional-structural 3D root models are promising tools for analyzing the diversity and complexity of root system phenotypes with Genotype × Environment interactions. The model parameters are assumed to be synthetic traits, less influenced by the environment, and consequently with less polygenic architectures than the integrative RSA traits they drive. Root models can serve as a basis for in silico development of root system ideotypes by highlighting the developmental processes and parameters that most likely influence RSA fitness.

Decoupling the effects of water and heat stress on Sauvignon blanc berries

Climate changes have important consequences in viticulture, heat waves accompanied by periods of drought are encountered more and more frequently. This study aims to evaluate the single and combined effect of water deficit and high temperatures on the thiol precursors biosynthesis in Sauvignon blanc grapes. For this purpose, a protocol has been developed for the cultivation of berries on a solid substrate. The berries, collected at three different times starting from veraison and grown in vitro, were subjected to 4 different treatments: control (C), water stress (WS), heat stress (HS), combined water and heat stress (WSHS). Water stress was simulated by adding abscisic acid to the culture medium, while different temperatures, respectively 25°C and 35°C, were managed with two illuminated climatic chambers.