terclim by ICS banner
IVES 9 IVES Conference Series 9 ASSESSMENT OF GRAPE QUALITY THROUGH THE MONITORING OFPHENOLIC RIPENESS AND THE APPLICATION OF A NEW RAPID METHOD BASED ON RAMAN SPECTROSCOPY

ASSESSMENT OF GRAPE QUALITY THROUGH THE MONITORING OFPHENOLIC RIPENESS AND THE APPLICATION OF A NEW RAPID METHOD BASED ON RAMAN SPECTROSCOPY

Abstract

The chemical composition of grape berries at harvest is one of the key aspects influencing wine quality and depends mainly on the ripeness level of grapes. Climate change affects this trait, unbalancing technological and phenolic ripeness, and this further raises the need for a fast determination of the grape maturity in order to quickly and efficiently determine the optimal time for harvesting. To this end, the characterization of variety-specific ripening curves and the development of new and rapid methods for determining grape ripeness are of key importance.

As part of this ongoing project, 35 vineyards (26 cv. Nebbiolo, 9 cv. Barbera) from Langhe, Roero, and Monferrato terroirs (Piemonte, Italy) were monitored during two consecutive vintages (2021-2022). The Nebbiolo vineyards were further classified, based on historical data, into ripening classes according to the harvest period estimation (early, medium, and late Nebbiolo). To study the evolution of grape ripening, four grape samples were taken from each vineyard during the ripening period (mid-August – late September), and grape quality assessment was performed by means of parameters commonly used in wine industry: juice technological maturity and phenolic ripeness parameters (total and extractable anthocyanins-EA%, share of tannins from seeds-Mp%). Preliminary results showed differences among cultivars and ripening classes, with a strong influence of the climatic conditions of the vintage, being both hot vintages with a strong water deficit (and decrease in berry weights and anthocyanin accumulation) for the 2022 vintage.

To have a more in-depth insight into the phenolic changes of the grapes during ripening, total extractions of the skins and seeds phenolics were carried out to better characterize the composition of Nebbiolo and Barbera berries. Lastly, this data was used to train a new approach based on Raman spectroscopy (RS), in an attempt to develop a method for the rapid determination of berry quality. At each sampling point, the acquisition of the grape Raman spectra was carried out in parallel with the other chemical analyses, developing a prediction model by correlating technological and phenolic ripening parameters with RS results.

Acknowledgments: The QUALSHELL project is funded by the PSR 2014-2020 Regione Piemonte (Italy), op. 16.1, European Agricultural Fund for Rural Development. We thank Martina Tarditi, Daniele Ronco, Alessandro Bottallo and the wineries supplying grape samples.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Lorenzo Ferrero¹, Alessio Sacco², Massimo Guaita³, Walter Salvano⁴, Andrea M. Rossi², Luca Rolle¹, Antonella Bosso³, Simone Giacosa¹

1. Università degli Studi di Torino, Dipartimento di Scienze Agrarie, Forestali e Alimentari. Corso Enotria 2/C, 12051 Alba, Italy
2. Istituto Nazionale di Ricerca Metrologica. Strada delle Cacce 91, 10135 Torino, Italy
3. Consiglio per la Ricerca e la Sperimentazione in Agricoltura, Centro di Ricerca Viticoltura ed Enologia. Via P. Micca 35, 14100 Asti, Italy
4. Terre del Barolo. Via Alba-Barolo 8, 12060 Castiglione Falletto, Italy

Contact the author*

Keywords

Grape quality, Phenolic ripeness, Anthocyanins, Red wines

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

Molecular approaches for understanding and modulating wine taste

Wine consumers generally demand wines having a perception of softer tannins and less ripe, having a heaviness and richness on palate (full-body wine) with a limpid and stable color. However, polyphenol
(tannins)-rich wines have been also correlated with unpleasant taste properties such as astringency and
bitterness when perceived at high intensities. Modulating these unpleasant properties could be important for consumer’s approval of wines.

OENOLOGICAL TANNINS FOR PREVENTING THE LIGHT-STRUCK TASTE IN WHITE AND ROSÉ WINES

The light exposure of wine can be detrimental as a relevant loss of aromas takes place [1] and light-induced reactions can occur. The latter involves riboflavin (RF), a photosensitive compound, that is fully reduced by acquiring two electrons. When the electron-donor is methionine, the light-struck taste (LST) can appear leading to cooked cabbage, onion and garlic odours-like [2]. The use of oenological tannins can limit the appearance of LST in both model wine [3] and white wine [4]. This research aimed to evaluate the impact of certain oenological tannins, selected in a previous study as the most effective against LST [5], in both white and rosé wines.

ANTHOCYANINS EXTRACTION FROM GRAPE POMACE USING EUTECTIC SOLVENTS

Grape pomace is one of the main by-products generated after pressing in winemaking.Emerging methods, such as ultrasound-assisted extraction with eutectic mixtures, have great potential due to their low toxicity, and high biodegradability. Choline chloride (ChCl) was used as a hydrogen bond acceptor and its corresponding hydrogen bond donor (malic acid, citric acid, and glycerol: urea). Components were heated at 80 °C and stirred until a clear liquid was obtained. Distilled water was added (30 % v/v). A solid-liquid ratio of 1 g pomace per 10 ml of eutectic solvent was used.

IMPACT OF NEW BIO STIMULANTS ON GRAPE SECONDARY METABOLITES UNDER CLIMATE CHANGE CONDITIONS

In a context of climate change and excessive use of agrochemical products, sustainable approaches for environmental and human health such as the use of bio stimulants in viticulture represent a potential option, against abiotic and biotic threats. Bio stimulants are organic compounds, microbes, or a combination of both, that stimulate plant’s vital processes, allowing high yields and good quality products. In vines, may trigger an innate immune response leading to the synthesis of secondary metabolites, key compounds for the organoleptic properties of grapes and wines.

AROMA ASSESSMENT OF COMMERCIAL SFORZATO DI VALTELLINA WINES BYINSTRUMENTAL AND SENSORY METHODOLOGIES

Sforzato di Valtellina DOCG is a special dry red wine produced from partially dehydrated Nebbiolo wine-grapes growing in the Rhaetian Alps valley of Valtellina (Lombardy, Italy). Valtellina terraced vineyards are located at an altitude of 350–800 m according to ‘heroic’ viticulture on steep slopes. The harvested grape bunches are naturally dehydrated indoors, where a slow and continuous withering occurs (about 20% w/w of weight loss), until at least 1st December when the grapes reach the desired sugar content and can be processed following a normal winemaking with maceration.