MICROFLUIDIC PLATFORM FOR SORTING YEAST CELLS ACCORDING TO THEIR MORPHOLOGY
In this work we briefly present a microfluidic device aiming to sort yeast cells according to their morphology. The technology is based upon microfluidic chips made out of Polydimethylsiloxane and glass using soft lithography processes and replica molding. The microfluidic device was used for encapsulating single yeast cells in liquid droplets containing growth medium. Liquid droplet containing yeast cells were sorted using a real time imaging and decision-making process. The technology used is based upon a home-made platform (labview based) for comparison of cells with recorded templates (1). Sorting is achieved by triggering electric fields on chip to actuate the hit droplets in the selection channels. Budding cells, ascospores, zygotes are differentiated at rates of ~30 drops/sec. From freshly mated strains, newly formed F1-zygotes can be sorted, while single ascus can be sorted from sporulating strains. Around 300 positive droplets with the desired event can be collected in a fraction tube using a single chip in one hour. This microfluidic platform constitutes an original device for achieving critical operation of yeast breeding at a Hight ought level.
1. Girault, M., Beneyton, T., del Amo, Y. & Baret, J. C. Microfluidic technology for plankton research. Curr. Opin. Biotechnol. 55, 134–150 (2019)
Issue: OENO Macrowine 2023
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breeding, yeast spores, hybridization, microfluidics