terclim by ICS banner
IVES 9 IVES Conference Series 9 PROFILING OF LIPIDS IN WINES FROM MONOCULTURE FERMENTATION WITH INDIGENOUS METSCHNIKOWIA YEAST SPECIES

PROFILING OF LIPIDS IN WINES FROM MONOCULTURE FERMENTATION WITH INDIGENOUS METSCHNIKOWIA YEAST SPECIES

Abstract

Lipids are a diverse group of organic compounds essential for living systems. They are vital compounds for yeast which makes them an important modulator of yeast metabolism in alcoholic fermentation. This study presents a comprehensive lipidome analysis of wine samples from the Vitis vinifera L., Maraština. The fermentation trails were set up in monoculture with different indigenous yeast strains selected from a collection of native yeasts established at the Institute for Adriatic Crops and Karst Reclamation in 2021, previously isolated from Croatian Maraština grapes: Metschnikowia pulcherrima, Metshnikowia sinensis/shanxiensis , and Metschnikowia chyrsoperlae. Commercial yeast M. pulcherrima FLAVIA served to control fermentation. Regarding the good sensitivity, specificity, and dynamic range of the UHPLC–MS/MS method, different classes of lipids were identified and quantified: free saturated fatty acids (6), free unsaturated fatty acids (5), triterpenoid (1), glycerophospholipid (1), glycerolipid (1), and free fatty acid esters (6). Methyl stearate was the only compound that is identified and quantified in wine but not found in must. The most abundant lipid compound in the Maraština musts and all experimental Maraština wines was palmitic acid (C16:0). Fermentation trials with M. chyrsoperlae yeast strain showed the highest concentrations of glycerolipid, triterpenoid, and free fatty acids. Mystric, linoleic acid, and glycerophospholipid had the highest concentrations in the fermentation with the M. sinensis/shanxiensis yeast strain, whereas M. pulcherima dominated in the production of ethyl free fatty esters.

 

1. Fahy, E.; Cotter, D.; Sud, M.; Subramaniam, S. Lipid classification, structures and tools. Biochim. Biophys.Acta-Mol. Cell Biol. Lipids 2011, 1811, 637-647.
2. Subramaniam, S.; Fahy, E.; Gupta, S.; Sud, M.; Byrnes, R. W.; Cotter, D.; Maurya, M. R. Bioinformatics and systems biology of the lipidome. Chemical Reviews 2011, 111(10), 6452-6490.
3. Pérez-Navarro, J.; Da Ros, A.; Masuero, D.; Izquierdo-Cañas, P. M.; Hermosín-Gutiérrez, I.; Gómez-Alonso, S.; Mattivi, F. LC-MS/MS analysis of free fatty acid composition and other lipids in skins and seeds of Vitis vinifera grape cultivars. Food Research International 2019, 125, 108556.
4. Della Corte, A.; Chitarrini, G.; Di Gangi, Iole M.; Masuero, D.; Soini, E.; Mattivi, F.; Vrhovšek, U. A rapid LC-MS/MS method for quantitative profiling of fatty acids, sterols, glycerolipids, glycerophospholipids and sphingolipids in grapes. Talanta, 2015, 140, 53-61.
5. Masuero, D.; Škrab, D.; Chitarrini, G.; Garcia-Aloy, M.; Franceschi, P.; Sivilotti, P.; Guella, G.; Vrhovsek, U. Grape lipidomics: an extensive profiling thorough UHPLC–MS/MS method. Metabolites 2021, 11(12), 827.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Ana BOBAN¹, Vesna MILANOVIò, Urska VRHOVSEK³, Domenico MASUERO³, Zvonimir JURUN¹, Irena BUDIĆ-LETO¹

1. Institute for Adriatic Crops and Karst Reclamation, 21 000 Split, Croatia
2. Dipartimento di Scienze Agrarie, Alimentari ed Ambientali, Universit`a Politecnica delle Marche, via Brecce Bianche, 60131 Ancona, Italy
3. Metabolomics Unit, Research and Innovation Centre, Fondazione Edmund Mach, 38098 San Michele all’Adige,TN, Italy

Contact the author*

Keywords

lipids, Metschnikowia species, indigenous yeast, Maraština wine

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

CHARACTERIZATION OF THE VOLATILE COMPOUNDS PROFILE OF COMMERCIAL GRAPPAS OBTAINED FROM THE POMACE OF AMARONE WINES

Grappa is a traditional Italian alcoholic beverage, with an alcohol content generally between 40-60% vol., obtained from the distillation of grape pomace used for the production of wine. Grappa are often aged in wooden barrels. There are various types of grappa: young, aromatic, aged, extra-aged depending on whether the distillate comes from aromatic vines or is aged in wooden barrels for shorter or longer periods. There is also flavored grappa if herbs, fruit or roots are added. All this makes it an extremely heterogeneous product both from an organoleptic and compositional point of view.

FOURIER TRANSFORM INFRARED SPECTROSCOPY IN MONITORING THE WINE PRODUCTION

The complexity of the wine matrix makes the monitoring of the winemaking process crucial. Fourier Transform Infrared Spectroscopy (FTIR) along with chemometrics is considered an effective analytical tool combining good accuracy, robustness, high sample throughput, and “green character”. Portable and non-portable FTIR devices are already used by the wine industry for routine analysis. However, the analytical calibrations need to be enriched, and some others are still waiting to be thoroughly developed.

OENOLOGICAL STRATEGIES FOR THE REMOVAL OF PINKING IN WHITE WINE

The pinking of in white wine is the turning of color from yellow to salmon hue. White wines obtained from certain grape varieties (e.g. Chardonnay, Sauvignon blanc, Riesling, Trebbiano di Lugana) showed to be susceptible to pinking [1] that has been evaluated by an assay providing the addition of hydrogen peroxide. Even if its appearance does not seem to affect the sensory properties [2], strategies are necessary for its removal. Nowadays, the treatment with polyvinylpolipirroline (PVPP) was reported to significantly decrease the pink color [3].

DEVELOPMENT OF DISTILLATION SENSORS FOR SPIRIT BEVERAGES PRODUCTION MONITORING BASED ON IMPEDANCE SPECTROSCOPY MEASUREMENT AND PARTIAL LEAST SQUARES REGRESSION (PLS-R)

During spirit beverages production, the distillate is divided in three parts: the head, the heart, and the tail. Acetaldehyde and ethanol are two key markers which allow the correct separation of distillate. Being toxic, the elimination of the head part, which contains high concentration of acetaldehyde, is crucial to guarantee the consumer’s health and security. Plus, the tail should be separated from the heart based on ethanol concentration.

INSIGHT THE IMPACT OF GRAPE PRESSING ON MUST COMPOSITION

The pre-fermentative steps play a relevant role for the characteristics of white wine [1]. In particular, the grape pressing can affect the chemical composition and sensory profile and its optimized management leads to the desired extraction of aromas and their precursors, and phenols resulting in a balanced wine [2-4]. These aspects are important especially for must addressed to the sparkling wine as appropriate extraction of phenols is expected being dependent to grape composition, as well.