terclim by ICS banner
IVES 9 IVES Conference Series 9 YEAST DERIVATIVE PRODUCTS: CHARACTERIZATION AND IMPACT ON RIBOFLAVIN RELEASE DURING THE ALCOHOLIC FERMENTATION

YEAST DERIVATIVE PRODUCTS: CHARACTERIZATION AND IMPACT ON RIBOFLAVIN RELEASE DURING THE ALCOHOLIC FERMENTATION

Abstract

Light-struck taste (LST) is a wine fault that can occur in white and sparkling wines when exposed to light. This defect is mainly associated to the formation of methanethiol and dimethyl disulfide due to light-induced reactions involving riboflavin (RF) and methionine [1]. The presence of RF in wine is mainly due to the metabolism of yeast [2] which fermenting activity can be favoured by using yeast derivative products (YDPs) as nutrients. Nonetheless, a previous study showed the addition of YDPs before the alcoholic fermentation (AF) led to higher concentrations of RF in wines [3]. Due to the widespread use of YDPs in the winemaking process, this study aimed to understand the possible relation between the content of RF in wine and the YDP adopted as nutrient for AF.

The experimental plan included (i) the characterization of selected YDPs and (ii) their addition as nutrient in microvinification trials using must as medium. Fourteen commercial YDPs of different nature (inactivated yeasts [IYs], mannoproteins [MPs], yeast autolysates [YAs], yeast extract [YEs] and yeast hulls [YHs]) were chosen. They were characterized in terms of flavins (RF, FAD and FMN), amino acid profile and sulfur-containing compounds (e.g. reduced glutathione [GSH], cysteine, cell wall cysteine and adsorbed cysteine). The characterized IYs, YEs and YHs were used for fermentation trials, carried out in Chardonnay must with 4 Saccharomyces cerevisiae strains. Flavins were quantified before and after the alcoholic fermentation.

Both YEs and one YA were the richest in RF which increase up to 20 μg/L was estimated considering an addition in must of 40 g/hL. These YDPs showed also the highest concentration of amino acids (up to 300 mg/L). However, among the flavins, FMN was the major one in most of the analyzed YDPs. With regards to GSH, this tripeptide was found at the highest concentration in the same YA (13.2 mg/g). The addition of YDPs caused a variation in RF released during AF depending on both the yeast strain and YDP nature. The addition of YEs caused an RF increase in must of about 15-20 μg/L, in accordance to RF content found in these products. For one of the yeast strains investigated, RF fatherly increased up to 30 μg/L during AF.

These results evidence the impact of YDPs on RF content indicating that the selection of nutrients combined with the choice of fermenting yeast strain should be considered for preventing the risk of LST appearance.

 

1. Fracassetti D., Di Canito A., Bodon R., Messina N., Vigentini I., Foschino R., Tirelli A. (2021).  Light-struck taste in white wine: Reaction mechanisms, preventive strategies and future perspectives to preserve wine quality. Trends in Food Science & Technology 112, 547-558. https://doi.org/10.1016/j.tifs.2021.04.013
2. Di Canito A., Altomare A., Fracassetti D., Messina N., Foschino R., Vigentini I. (2023). The riboflavin metabolism in four Saccharomyces cerevisiae wine strains: assessment in oenological condition and potential implications with the light-struck taste. Journal of Fungi 9 (2023), 78. https://doi.org/10.3390/jof9010078
3. Fracassetti D., Gabrielli M., Encinas J., Manara M., Pellegrino I., Tirelli A. (2017). Approaches to prevent the light-struck taste in white wine. Australian Journal of Grape and Wine Research 23, 329–333, 2017. https://doi.org/10.1111/ajgw.12295

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Alessio Altomare¹, Alessandra di Canito², Ileana Vigentini², Roberto Foschino², Antonio Tirelli¹, Daniela Fracassetti¹*

1. Department of Food, Environmental and Nutritional Sciences (DeFENS), Università degli Studi di Milano, Via G. Celoria 2, 20133 Milan, Italy
2. Department of Biomedical, Surgical and Dental Sciences (DSBCO), Università degli Studi di Milano, Via della Commenda 10, 20122 Milan, Italy – *Corresponding author

Contact the author*

Keywords

Light-struck taste, Flavins, Gluthathione, Must

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

WINE AS AN EMOTIONAL AND AESTHETIC OBJECT: IMPACT OF EXPERTISE

Wine tasting has been shown to provide emotions to tasters (Coste et al. 2018). How will expertise impact this emotional response? Burnham and Skilleås (2012) reported that the cultural, experiential, and aesthetic competencies characterize an expert in wine compared to a novice. Although there is no consensual definition of an aesthetic experience, Burnham and Skilleås (2012) reported that aesthetic appreciation is “disinterested, normative for others and communicable” in comparison to sensory pleasure.

IMPACT OF RHIZOPUS AND BOTRYTIS ON WINE FOAMING PROPERTIES

A lot of work has been done on the impact of Botrytis on the foam of sparkling wines. This work often concerns wines produced in cool regions, where Botrytis is the dominant fungal pathogen. However, in southern countries such as Spain, in particularly hot years such as 2022, the majority fungal pathogen is sometimes Rhizopus. Like Botrytis, Rhizopus is a fungus that produces an aspartic protease.

HOW DO ROOTSTOCKS AFFECT CABERNET SAUVIGNON AROMATIC EXPRESSION?

Grape quality potential for wine production is strongly influenced by environmental parameters such as climate and agronomic factors such as rootstock. Several studies underline the effect of rootstock on vegetative growth of the scions [1] and on berry composition [2, 3] with an impact on wine quality. Rootstocks are promising agronomic tools for climate change adaptation and in most grape-growing regions the potential diversity of rootstocks is not fully used and only a few genotypes are planted. Little is known about the effect of rootstock genetic variability on the aromatic composition in wines; thus further investigations are needed.

THE EFFECT OF COPPER ON THE PRODUCTION OF VARIETAL THIOLS DURING THE ALCOHOLIC FERMENTATION OF COLOMBARD AND GROS MANSENG GRAPE JUICES

Nowadays, the rapid growth of vineyards with organic practices and the use of copper as the only fun-gicide against downy mildew raises again the question of the effect of copper on varietal thiols in wine, especially 3-sulfanylhexan-1-ol (3SH) and its acetate (3SHA). A few decades ago, several works indicated that the use of copper in the vineyard had a negative effect on the content of varietal thiols in Sauvignon blanc wines [1, 2]. However, these studies only considered the concentration of the reduced form (RSH) of varietal thiols, without quantifying the oxidised ones. For this purpose, we proposed to monitor both reduced and oxidised forms of varietal thiols in wine under copper stress during alcoholic fermentation to have a more complete picture of the biological and chemical mechanisms.

CONSUMER PERCEPTION OF INTERSPECIFIC HYBRID RED WINE COLOR IN RELATION TO ANTHOCYANIN PROFILE AND CHEMICAL COLOR PARAMETERS

Interspecific hybrid winegrapes are of growing interest in the context of climate change based on their disease resistance and cold hardiness. In addition to a need for increased understanding of their chemical composition, there is little empirical evidence on the consumer perception of non-vinifera wine. Phenolic compounds, and particularly color, play an important organoleptic and quality determination role in wine, but can vary significantly in interspecific hybrid wines compared to wines produced from Vitis vinifera cultivars [1, 2, 3]. Anecdotally, the variation in anthocyanin species, interactions, and concentrations in interspecific hybrids could result in a variance from“vinifera-like” wine color.