terclim by ICS banner
IVES 9 IVES Conference Series 9 RED WINE AGING THROUGH 1H-NMR METABOLOMICS

RED WINE AGING THROUGH 1H-NMR METABOLOMICS

Abstract

Premium red wines are often aged in oak barrel. This widespread winemaking process is used, among others, to provide roundness and complexity to the wine. The study of wine evolution during barrel aging is crucial to better ensure control of wine quality.

¹H-NMR has already been proved to be an efficient tool to monitor winemaking process [1]. Indeed, it is a non-destructive technique, it requires a small amount of sample and a short time of analysis, yet it provides clues about several chemical families. The aim of the present study is to investigate the evolution of wine during aging in oak barrels with NMR-based metabolomics.

Red wines, produced in an estate of Bordeaux region, were kept in oak barrels from three different manufacturers. They were firstly sampled after one month of aging. They were then resampled after twelve months of storage in oak barrels within the estate cellar. The evolution of wine constituents during aging was measured by ¹H-NMR-based metabolomics. NMR spectra were submitted to targeted and untargeted approaches.

Data were then statistically processed through multivariate statistical analysis such as principal component analysis (PCA), and orthogonal projections to latent structures discriminant analysis (OPLS-DA). It was used to better watch the distribution of metabolic variance, and to sharpen the separation between observations groups. The results of supervised models were validated using cross permutation tests and ANOVA. Statistical significances were then assessed for the potential discriminant compounds thanks to analysis of variance (ANOVA) or t-test. Based on this analysis, wine maturation effect was monitored, and discriminant metabolites were identified.

Regarding aging effect, wines analyzed after one month of aging exhibit higher contents of amino acids, catechin and epicatechin, acetoin and choline. On another side, wines analyzed after twelve months of aging present higher contents of acetic acid, ethyl lactate, arabinose, and glucose.

As it concerns barrel origins, samples showed higher heterogeneity after one month than after twelve months. However, significant differences were observed between wines depending on the barrel manufacturers.

 

1. Le Mao, I., Da Costa, G., & Richard, T. (2023). 1 H-NMR metabolomics for wine screening and analysis. OENO One, 57(1), 15-31. https://doi.org/10.20870/oeno-one.2023.57.1.7134 

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Guillaume Leleu, Gregory Da Costa, Inès Le Mao, Tristan Richard

University of Bordeaux, Bordeaux INP, UMR OENO, UMR 1366, ISVV, F-33140 Villenave d’Ornon, France

Contact the author*

Keywords

wine aging, NMR metabolomics, oak barrels, fingerprinting

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

IMPACT OF MINERAL AND ORGANIC NITROGEN ADDITION ON ALCOHOLIC FERMENTATION WITH S. CEREVISIAE

During alcoholic fermentation, nitrogen is one of essential nutrient for yeast as it plays a key role in sugar transport and biosynthesis of and wine aromatic compounds (thiols, esters, higher alcohols). The main issue of a lack in yeast assimilable nitrogen (YAN) in winemaking is sluggish or stuck fermentations promoting the growth of alteration species and leads to economic losses. Currently, grape musts are often characterized by low YAN concentration and an increase of sugars concentration due to global warming, making alcoholic fermentations even more difficult. YAN depletion can be corrected by addition of inorganic (ammonia) or organic (yeast derivatives products) nitrogen during alcoholic fermentation.

HYBRID GRAPEVINE CV BACO BLANC, BETWEEN TRADITION AND MODERNISM: FOCUS ON ENDOGENOUS EUGENOL AS RESISTANCE FACTOR TO BOTRYTIS CINEREA

The well-known antifungal and antibiotic molecule, eugenol, is widely spread in various plants including clove, basil and bay. It is also abundant in the hybrid grapevine cultivar (cv) Baco blanc (Vitis vi-nifera x Vitis riparia x Vitis labrusca), created by François Baco (19th century) in the Armagnac region. This study confirmed this cv as highly resistant to Botrytis cinerea by comparing fruit rot incidence and severity with two Vitis vinifera cultivars: Folle Blanche and Ugni Blanc. We have demonstrated the efficiency of eugenol in vitro, by further investigating the effect of small concentrations of eugenol, 3 to 4 ppm (corresponding to IC10), on B. cinerea. By comparing the two major modes of action (direct or volatile antibiosis), the vapour inhibiting effect of eugenol was more powerful. In the skin of Baco blanc berry, the total eugenol concentration reached a maximum at veraison, i.e. 1118 to 1478 μg/kg.

Influence of agrophotovoltaic on vine and must in a cool climate

The current energy crisis means that interest in agrophotovoltaics has increased significantly. The reason behind this is that the system aims to combine agricultural production with energy production. During the three-year period from 2020 to 2022, the effects of photovoltaic panels on the vine, the yield and the quality of the must were studied in Walenstadt in northern Switzerland, an area with a cool, humid climate. 65 Pinot noir vines were planted in the 160m2 study area. Because of the large edge effects, only 3 repetitions with 4 vines each could be created. A significantly lower leaf infestation by Plasmopara viticola was observed among the panels in each of the three years.

NEW PLANT BIOPOLYMERS FOR THE COLLOIDAL STABILITY OF THE COLORING MATTER OF RED WINES

The color as well as the “clarity” of red wines are ones of the qualities required by the consumers. Red wines must have colloidal stability from its bottling to its consumption. The supplementation of red wines with additives, and especially Acacia senegal gum, contributes to its organoleptic properties such as the colloidal stabilization of the coloring matter. In a global perspective of limitation of additives in the field of enology, one of the objectives is notably (i) to reduce the use of additives in wines, by their number and/or their quantity, and (ii) to favor the use of natural additives while preserving the organoleptic and sensory qualities of wines.

INSIGHT THE IMPACT OF GRAPE PRESSING ON MUST COMPOSITION

The pre-fermentative steps play a relevant role for the characteristics of white wine [1]. In particular, the grape pressing can affect the chemical composition and sensory profile and its optimized management leads to the desired extraction of aromas and their precursors, and phenols resulting in a balanced wine [2-4]. These aspects are important especially for must addressed to the sparkling wine as appropriate extraction of phenols is expected being dependent to grape composition, as well.