terclim by ICS banner
IVES 9 IVES Conference Series 9 RED WINE AGING THROUGH 1H-NMR METABOLOMICS

RED WINE AGING THROUGH 1H-NMR METABOLOMICS

Abstract

Premium red wines are often aged in oak barrel. This widespread winemaking process is used, among others, to provide roundness and complexity to the wine. The study of wine evolution during barrel aging is crucial to better ensure control of wine quality.

¹H-NMR has already been proved to be an efficient tool to monitor winemaking process [1]. Indeed, it is a non-destructive technique, it requires a small amount of sample and a short time of analysis, yet it provides clues about several chemical families. The aim of the present study is to investigate the evolution of wine during aging in oak barrels with NMR-based metabolomics.

Red wines, produced in an estate of Bordeaux region, were kept in oak barrels from three different manufacturers. They were firstly sampled after one month of aging. They were then resampled after twelve months of storage in oak barrels within the estate cellar. The evolution of wine constituents during aging was measured by ¹H-NMR-based metabolomics. NMR spectra were submitted to targeted and untargeted approaches.

Data were then statistically processed through multivariate statistical analysis such as principal component analysis (PCA), and orthogonal projections to latent structures discriminant analysis (OPLS-DA). It was used to better watch the distribution of metabolic variance, and to sharpen the separation between observations groups. The results of supervised models were validated using cross permutation tests and ANOVA. Statistical significances were then assessed for the potential discriminant compounds thanks to analysis of variance (ANOVA) or t-test. Based on this analysis, wine maturation effect was monitored, and discriminant metabolites were identified.

Regarding aging effect, wines analyzed after one month of aging exhibit higher contents of amino acids, catechin and epicatechin, acetoin and choline. On another side, wines analyzed after twelve months of aging present higher contents of acetic acid, ethyl lactate, arabinose, and glucose.

As it concerns barrel origins, samples showed higher heterogeneity after one month than after twelve months. However, significant differences were observed between wines depending on the barrel manufacturers.

 

1. Le Mao, I., Da Costa, G., & Richard, T. (2023). 1 H-NMR metabolomics for wine screening and analysis. OENO One, 57(1), 15-31. https://doi.org/10.20870/oeno-one.2023.57.1.7134 

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Guillaume Leleu, Gregory Da Costa, Inès Le Mao, Tristan Richard

University of Bordeaux, Bordeaux INP, UMR OENO, UMR 1366, ISVV, F-33140 Villenave d’Ornon, France

Contact the author*

Keywords

wine aging, NMR metabolomics, oak barrels, fingerprinting

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

FREE TERPENE RESPONSE OF ‘MOSCATO BIANCO’ VARIETY TO GRAPE COLD STORAGE

Temperature control is crucial in wine production, starting from grape harvest to the bottled wine storage. Climate change and global warming affect the timing of grape ripening, and harvesting is often done during hot summer days, influencing berry integrity, secondary metabolites potential, enzyme and oxidation phenomena, and even fermentation kinetics. To curb this phenomenon, pre-fermentative cold storage can help preserve the grapes and possibly increase the concentration of key secondary metabolites. In this study, the effect of grape pre-fermentative cold storage was assessed on the ‘Moscato bianco’ white grape cultivar, known for its varietal terpenes (65% of free terpenes represented by linalool and its derivatives) and widely used in Piedmont (Italy) to produce Asti DOCG wines.

SENSORY EVALUATION OF WINE AROMA: SHOULD COLOR-DRIVEN DESCRIPTORS BE USED?

The vocabulary used to describe wine aroma is commonly organized according to color, raising the question of whether they reflect the reality of olfactory perception. Previous studies have assumed this convention of color-aroma matching, and have investigated color’s influence on the perception of aroma only in dyed white wine or in red wine from particular places of origin. Here 48 white and red varietal wines from around the world were evaluated in black glasses then in clear glasses by a panel of wine experts, who gave intensity ratings for aroma attributes commonly used by wine professionals. In black glasses, aromas conventionally associated with white wine were perceived in the red wines, and vice versa.

WINE AS AN EMOTIONAL AND AESTHETIC OBJECT: IMPACT OF EXPERTISE

Wine tasting has been shown to provide emotions to tasters (Coste et al. 2018). How will expertise impact this emotional response? Burnham and Skilleås (2012) reported that the cultural, experiential, and aesthetic competencies characterize an expert in wine compared to a novice. Although there is no consensual definition of an aesthetic experience, Burnham and Skilleås (2012) reported that aesthetic appreciation is “disinterested, normative for others and communicable” in comparison to sensory pleasure.

ESTIMATING THE INITIAL OXYGEN RELEASE (IOR) OF CORK CLOSURES

Many factors influence aging of bottled wine, oxygen transfer through the closure is included. The maximum uptake of wine before oxidation begins varies from 60 mg.L-¹ to 180 mg.L-1 for white and red wines respectively [1].
The process of bottling may lead to considerable amounts of oxygen. The actual contribution of the transfer through the closure system becomes relevant at the bottle storage, but the amounts are small compared to prepacking operations [2] and to the total oxygen attained during filling.

Grouping Vitis vinifera grapevine varieties based on their aromatic composition

Climate change is likely to impact wine typicity across the globe, raising concerns in wine regions historically renowned for the quality of their terroir1. Amongst several changes in viticultural practices, replacing some of the planting material (i.e. clones, rootstocks and cultivars) is thought to be one of the most promising potential levers to be used for adapting to climate change. But the change of cultivars also involves the issue of protecting the region’s wine typicity. In Bordeaux (France), extensive research has been conducted on identifying meridional varieties that could be good candidates to help guard against the effects of climate change2 while less research has been done concerning their impacts on Bordeaux wine typicity.