terclim by ICS banner
IVES 9 IVES Conference Series 9 RED WINE AGING THROUGH 1H-NMR METABOLOMICS

RED WINE AGING THROUGH 1H-NMR METABOLOMICS

Abstract

Premium red wines are often aged in oak barrel. This widespread winemaking process is used, among others, to provide roundness and complexity to the wine. The study of wine evolution during barrel aging is crucial to better ensure control of wine quality.

¹H-NMR has already been proved to be an efficient tool to monitor winemaking process [1]. Indeed, it is a non-destructive technique, it requires a small amount of sample and a short time of analysis, yet it provides clues about several chemical families. The aim of the present study is to investigate the evolution of wine during aging in oak barrels with NMR-based metabolomics.

Red wines, produced in an estate of Bordeaux region, were kept in oak barrels from three different manufacturers. They were firstly sampled after one month of aging. They were then resampled after twelve months of storage in oak barrels within the estate cellar. The evolution of wine constituents during aging was measured by ¹H-NMR-based metabolomics. NMR spectra were submitted to targeted and untargeted approaches.

Data were then statistically processed through multivariate statistical analysis such as principal component analysis (PCA), and orthogonal projections to latent structures discriminant analysis (OPLS-DA). It was used to better watch the distribution of metabolic variance, and to sharpen the separation between observations groups. The results of supervised models were validated using cross permutation tests and ANOVA. Statistical significances were then assessed for the potential discriminant compounds thanks to analysis of variance (ANOVA) or t-test. Based on this analysis, wine maturation effect was monitored, and discriminant metabolites were identified.

Regarding aging effect, wines analyzed after one month of aging exhibit higher contents of amino acids, catechin and epicatechin, acetoin and choline. On another side, wines analyzed after twelve months of aging present higher contents of acetic acid, ethyl lactate, arabinose, and glucose.

As it concerns barrel origins, samples showed higher heterogeneity after one month than after twelve months. However, significant differences were observed between wines depending on the barrel manufacturers.

 

1. Le Mao, I., Da Costa, G., & Richard, T. (2023). 1 H-NMR metabolomics for wine screening and analysis. OENO One, 57(1), 15-31. https://doi.org/10.20870/oeno-one.2023.57.1.7134 

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Guillaume Leleu, Gregory Da Costa, Inès Le Mao, Tristan Richard

University of Bordeaux, Bordeaux INP, UMR OENO, UMR 1366, ISVV, F-33140 Villenave d’Ornon, France

Contact the author*

Keywords

wine aging, NMR metabolomics, oak barrels, fingerprinting

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

METHYL SALICYLATE: A TRENDY COMPOUND MARKER OF ZELEN, A UNIQUE SLOVENIAN VARIETY

The wine market interest for autochthonous varieties, particularly from less known wine regions, has significantly raised in the past few years. In that context, Slovenia, a small country from central Europe with a long winemaking tradition, is getting more and more attention, particularly through its range of unique regional varieties. Among them, Zelen, meaning “green” in Slovene, can only be found in the Vipava valley region, located on the western side of the country, near the border with Italy. When they are young, Zelen wines display very singular aromas reminiscent of rosemary, sage and white fruit. Despite its uniqueness, Zelen wine aromatic typicality is poorly documented in the literature.

Microbial ecosystems in wineries – molecular interactions between species and modelling of population dynamics

Microbial ecosystems are primary drivers of viticultural, oenological and other cellar-related processes
such as wastewater treatment. Metagenomic datasets have broadly mapped the vast microbial species
diversity of many of the relevant ecological niches within the broader wine environment, from vineyard
soils to plants and grapes to fermentation. The data highlight that species identities and diversity
significantly impact agronomic performance of vineyards as well as wine quality, but the complexity
of these systems and of microbial growth dynamics has defeated attempts to offer actionable
tools to guide or predict specific outcomes of ecosystem-based interventions.

PROFILING OF LIPIDS IN WINES FROM MONOCULTURE FERMENTATION WITH INDIGENOUS METSCHNIKOWIA YEAST SPECIES

Lipids are a diverse group of organic compounds essential for living systems. They are vital compounds for yeast which makes them an important modulator of yeast metabolism in alcoholic fermentation. This study presents a comprehensive lipidome analysis of wine samples from the Vitis vinifera L., Maraština. The fermentation trails were set up in monoculture with different indigenous yeast strains selected from a collection of native yeasts established at the Institute for Adriatic Crops and Karst Reclamation in 2021, previously isolated from Croatian Maraština grapes: Metschnikowia pulcherrima, Metshnikowia sinensis/shanxiensis , and Metschnikowia chyrsoperlae.

AN AUTOMATIC CANOPY COOLING SYSTEM TO COPE WITH THE THERMAL-RADIATIVE STRESSES IN THE PIGNOLETTO WHITE GRAPE

In recent years characterized by hot dry summers, the implementation of innovative irrigation tools in the vineyard represents a crucial challenge to ensure optimal production and to avoid excess of water consumption. It is known that the grapevine reacts to multiple stresses – i.e., high temperatures and wa- ter shortage – through adaptive mechanisms that are detrimental to the yield. Furthermore, this condi- tion is usually aggravated by high solar radiation, which could negatively affect the phenolic composi- tion of the grapes. Therefore, a cooling system has been developed aiming to reduce bunches’ sunburn damage.

OENOLOGICAL AND SUSTAINABILITY POTENTIAL OF WINES PRODUCED FROM DISEASE RESISTANT GRAPE CULTIVARS (PIWI WINES)

The strategy for sustainability in the wine sector of the EU refers to a set of practices and principles that aim to minimize the negative impact of wine production on the environment, social and economic sustainability. Sustainable wine production involves a range of practices that are designed to reduce waste, conserve resources, and promote the well-being of workers and communities.