Macrowine 2021
IVES 9 IVES Conference Series 9 Extraction of pathogenesis-related proteins and phenolics in Sauvignon Blanc as affected by different

Extraction of pathogenesis-related proteins and phenolics in Sauvignon Blanc as affected by different

Abstract

The composition of wine is largely determined by the composition of pre-fermentation juice, which is influenced by extraction of grape components. Different grape harvesting and processing conditions could affect the extraction of grape components into juice. Among these grape components, pathogenesis-related (PR) proteins are of great concern for white wine maker as they are the main cause of haze formation in finished white wine. If not removed before bottling, these PR proteins may progress into haze through the formation of complex with phenolics under certain conditions. Thaumatin-like proteins (TLPs) and chitinases are the main constituents of PR proteins found in protein haze. The aim of this study was to investigate the effects of grape harvesting and processing conditions on extraction of haze-forming PR proteins and phenolics, and the consequent impact on protein stability of wine. Sauvignon Blanc grapes were harvested by hand and by machine in 2011 and 2012 in Marlborough, New Zealand. Hand harvested grapes were processed by two different treatments: whole bunch pressing without skin contact, and pressing after destemming and crushing followed by 3 h skin contact; machine harvested grapes were processed by pressing after destemming and crushing followed by 3 h skin contact. For each treatment, the juice pressing procedure was carried out by gradually increasing the pressing pressure to three different levels (0.4 MPa, 0.8 MPa and 1.6 MPa), and juice samples were collected at each pressure point and bottle-fermented separately. Results of this study showed that pressing after destemming and crushing followed by 3 h skin contact can result in greater extraction of proteins and phenolics from grapes into juice compared with whole bunch pressing. Juice and wine obtained from machine harvested grapes showed a tendency of having lower concentration of proteins, as well as, PR proteins. Juice and wine obtained from higher pressing pressure showed the highest concentration of phenolics but the lowest concentration of proteins, suggesting that proteins in grapes are easily extracted at low pressing pressure, but greater extraction of phenolics in grapes requires higher pressing pressure. Analysis of wine protein stability showed a linear correlation between bentonite requirement and the concentration of chitinases in wine, indicating the importance of removal of chitinases to achieve protein stabilization. The findings presented here contribute to an improved understanding of the variable concentration of haze-forming PR proteins in juice as affected by grape harvesting and processing conditions, and hence the variation in bentonite requirement for resultant wine.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Article

Authors

Bin Tian*, Claire Grose, James Morton, Marlene Jaspers, Mike Trought, Roland Harrison

*Lincoln University

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Quantification of red wine phenolics using ultraviolet-visible, near and mid-infrared spectroscopy combined with chemometrics

The use of multivariate statistics to correlate chemical data to spectral information seems as a valid alternative for the quantification of red wine phenolics. The advantages of these techniques include simplicity and cost effectiveness together with the limited time of analysis required. Although many
publications on this subject are nowadays available in the literature most of them only reported feasibility
studies. In this study 400 samples from thirteen fermentations including five different cultivars plus 150
wine samples from a varying number of vintages were submitted to spectrophotometric and chromatographic phenolic analysis.

On the losses of dissolved CO2 during champagne aging

A misconception lingers in the minds of some wine consumers that Champagne wines don’t age. It’s largely a myth, certainly as far as the best cuvees are concerned. Actually, during the so-called autolysis period of time (in the closed bottle, after the “prise de mousse”), complex chemical reactions take place when the wine remains in contact with the dead yeast cells, which progressively bring complex and very much sought-after aromas to champagne. Nevertheless, despite their remarkable impermeability to liquid and air, caps or natural cork stoppers used to cork the bottles are not 100% hermetic with regard to gas transfers. Gas species therefore very slowly diffuse through the cap or cork stopper, along their respective inverse partial pressure. After the “prise de mousse”, because the partial pressure of CO2 in the bottleneck reaches up to 6 bars (at 12 °C), gaseous CO2 progressively diffuse from the bottle to the ambient air
(where the partial pressure of gaseous CO2 is only of order of 0,0004 bar).

Effect of malolactic fermentation in barrels or stainless steel tanks on wine composition. Influence of the barrel toasting

Ellagitannin, anthocyanin and woody volatile composition of Cabernet Sauvignon wines aged in oak barrels for 12 months was evaluated. Depending on the container where malolactic fermentation (MLF) was carried out, two wine modalities were investigated: wines with MLF carried out in stainless steel tanks and barrel-fermented wines. Three toasting methods (medium toast, MT; medium toast with watering, MTAA; noisette) were considered for ageing of each wine modality. Sensory analyses (triangle and rating tests) were also performed. Two-way ANOVA of the raw experimental data revealed that the toasting method and the container where MLF took place, as well as the interaction between both factors, have a significant influence (p < 0.05) on ellagitannin, anthocyanin and woody volatile profiles of Cabernet Sauvignon wines.

Effects of a new vacuum evaporation method on chemical and sensory properties of must and wine

A new process for vacuum evaporation was developed where evaporation takes place near the inner surface of a vortex produced by a rotor submerged in the liquid. Contrary to the state of the art the Vortex rotor process does not need a vacuum vessel but the rotating liquid creates a geometrically stable low pressure void surrounded by a vortex stabilized by the equilibrium between centrifugal forces and the pressure difference. First tests with water and sugar solutions at concentrations similar to grape must were conducted to verify the theoretical predictions, test the performance under different conditions and study the effect of various process parameters (Rösti et al 2015).

Bentonite fining in cold wines: prediction tests, reduced efficiency and possibilities to avoid additional fining treatments

Bentonite fining is widely used to prevent protein haze in white wines. Most wineries use laboratory-scale fining trials to define the appropriate amount of bentonite to be used in the cellar. Those pre-tests need to mimic as much as possible the industrial scale fining procedure to determine the exact amount of bentonite necessary for protein stability. Nevertheless it is frequent that, after fining with the recommended amount of bentonite, wines appear still unstable and need an additional fining treatment. It remains a major challenge to understand why the same wine, fined with the same dosage of the same bentonite, achieves stability in the lab, but not in the cellar.