Macrowine 2021
IVES 9 IVES Conference Series 9 Extraction of pathogenesis-related proteins and phenolics in Sauvignon Blanc as affected by different

Extraction of pathogenesis-related proteins and phenolics in Sauvignon Blanc as affected by different

Abstract

The composition of wine is largely determined by the composition of pre-fermentation juice, which is influenced by extraction of grape components. Different grape harvesting and processing conditions could affect the extraction of grape components into juice. Among these grape components, pathogenesis-related (PR) proteins are of great concern for white wine maker as they are the main cause of haze formation in finished white wine. If not removed before bottling, these PR proteins may progress into haze through the formation of complex with phenolics under certain conditions. Thaumatin-like proteins (TLPs) and chitinases are the main constituents of PR proteins found in protein haze. The aim of this study was to investigate the effects of grape harvesting and processing conditions on extraction of haze-forming PR proteins and phenolics, and the consequent impact on protein stability of wine. Sauvignon Blanc grapes were harvested by hand and by machine in 2011 and 2012 in Marlborough, New Zealand. Hand harvested grapes were processed by two different treatments: whole bunch pressing without skin contact, and pressing after destemming and crushing followed by 3 h skin contact; machine harvested grapes were processed by pressing after destemming and crushing followed by 3 h skin contact. For each treatment, the juice pressing procedure was carried out by gradually increasing the pressing pressure to three different levels (0.4 MPa, 0.8 MPa and 1.6 MPa), and juice samples were collected at each pressure point and bottle-fermented separately. Results of this study showed that pressing after destemming and crushing followed by 3 h skin contact can result in greater extraction of proteins and phenolics from grapes into juice compared with whole bunch pressing. Juice and wine obtained from machine harvested grapes showed a tendency of having lower concentration of proteins, as well as, PR proteins. Juice and wine obtained from higher pressing pressure showed the highest concentration of phenolics but the lowest concentration of proteins, suggesting that proteins in grapes are easily extracted at low pressing pressure, but greater extraction of phenolics in grapes requires higher pressing pressure. Analysis of wine protein stability showed a linear correlation between bentonite requirement and the concentration of chitinases in wine, indicating the importance of removal of chitinases to achieve protein stabilization. The findings presented here contribute to an improved understanding of the variable concentration of haze-forming PR proteins in juice as affected by grape harvesting and processing conditions, and hence the variation in bentonite requirement for resultant wine.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Article

Authors

Bin Tian*, Claire Grose, James Morton, Marlene Jaspers, Mike Trought, Roland Harrison

*Lincoln University

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Effects of bottle closure type on sensory characteristics of Chasselas wines

Several winemaking operations, such as filtration, pumping, and racking, are known to potentially facilitate the incorporation of atmospheric O2 into the wine. Control of grape must oxidation is one key aspect in the management of white wine aroma expression, color stability and shelf-life extension. On the one hand, controlled must oxidation may help to remove highly reactive phenolic compounds, which otherwise could contribute to premature oxidation. And on the other hand, in certain cases of extreme protection of the must from O2 (e.g. pressing under inert atmosphere), it can help to preserve varietal aromas and natural must antioxidants.

Impact of non-fruity compounds on red wines fruity aromatic expression: the role of higher alcohols

A part, at least, of the fruity aroma of red wines is the consequence of perceptive interactions between various aromatic compounds, particularly ethyl esters and acetates, which may contribute to the perception of fruity aromas, specifically thanks to synergistic effects.1,2 The question of the indirect impact of non-fruity compounds on this particular aromatic expression has not yet been widely investigated. Among these compounds higher alcohols (HA) represent the main group, from a quantitative standpoint, of volatiles in many alcoholic beverages. Moreover, some bibliographic data suggested their contribution to the aromatic complexity by either increasing or masking flavors of wine, depending of their concentrations.

Evaluating South African Chenin blanc wine styles using an LC-MS screening method

Sorting Chenin blanc is one of the most important white wine cultivars in South Africa. It has received a lot of attention and accolades in the past years and more research than ever is dedicated to this versatile cultivar. According to the Chenin blanc association of South Africa, there are three recognized dry wine styles, Fresh and Fruity (FF), Rich and Ripe Unwooded
(RRU), and Rich and Ripe Wooded (RRW). They are traditionally established with the aid of expert sensory evaluation, but the cost and the (subjective) human factor are aspects to be taken into account. A more objective and possibly robust way of assessing and attributing these styles can be the use of chemical analysis.

Study of the content of amino acids and biogenic amines in sparkling red wines

The production of red sparkling wines is lower in Spain in comparison with the winemaking of white or rosé sparkling wines. In red sparkling wine processing it is essential to obtain suitable base wines that should have moderate alcohol content, high acidity, good color values, an adequate mouth-feel and a sweet tannin. Grapes for sparkling wine production have to be harvested at low maturity stages, with lower alcohol contents and higher acidities, which will that the phenolic maturity of the grapes is also low, showing green tannins. This paper analyses different treatments in order to minimize these inconveniences: cold maceration-prefermentation and delestage to elaborate the grapes with lower maturity, must nanofiltration, and the partial osmosis of the wines made from grapes with an adequate maturity degree.

Characterization of various groups of pyranoanthocyanins in Merlot red wine

In red wines, anthocyanins evolve during the wine-making process and ageing. They react with other compounds (such as vinylphenols, acetaldehyde, pyruvic acid…) to form a stable family of compounds called pyranoanthocyanins. Furthermore, the oxidation process can modify the anthocyanic profile of a red wine. It is also interesting to evaluate the occurrence of the different subclasses of pyranoanthocyanins and to characterize their chemical properties. The first objective of this study is to evaluate the occurrence of the different groups of pyranoanthocyanins in an oxidised Merlot wine by a centrifugal partition chromatography strategy. The second goal is to evaluate their relative impact in red wines from Bordeaux region by measuring their concentrations.