Macrowine 2021
IVES 9 IVES Conference Series 9 Extraction of pathogenesis-related proteins and phenolics in Sauvignon Blanc as affected by different

Extraction of pathogenesis-related proteins and phenolics in Sauvignon Blanc as affected by different

Abstract

The composition of wine is largely determined by the composition of pre-fermentation juice, which is influenced by extraction of grape components. Different grape harvesting and processing conditions could affect the extraction of grape components into juice. Among these grape components, pathogenesis-related (PR) proteins are of great concern for white wine maker as they are the main cause of haze formation in finished white wine. If not removed before bottling, these PR proteins may progress into haze through the formation of complex with phenolics under certain conditions. Thaumatin-like proteins (TLPs) and chitinases are the main constituents of PR proteins found in protein haze. The aim of this study was to investigate the effects of grape harvesting and processing conditions on extraction of haze-forming PR proteins and phenolics, and the consequent impact on protein stability of wine. Sauvignon Blanc grapes were harvested by hand and by machine in 2011 and 2012 in Marlborough, New Zealand. Hand harvested grapes were processed by two different treatments: whole bunch pressing without skin contact, and pressing after destemming and crushing followed by 3 h skin contact; machine harvested grapes were processed by pressing after destemming and crushing followed by 3 h skin contact. For each treatment, the juice pressing procedure was carried out by gradually increasing the pressing pressure to three different levels (0.4 MPa, 0.8 MPa and 1.6 MPa), and juice samples were collected at each pressure point and bottle-fermented separately. Results of this study showed that pressing after destemming and crushing followed by 3 h skin contact can result in greater extraction of proteins and phenolics from grapes into juice compared with whole bunch pressing. Juice and wine obtained from machine harvested grapes showed a tendency of having lower concentration of proteins, as well as, PR proteins. Juice and wine obtained from higher pressing pressure showed the highest concentration of phenolics but the lowest concentration of proteins, suggesting that proteins in grapes are easily extracted at low pressing pressure, but greater extraction of phenolics in grapes requires higher pressing pressure. Analysis of wine protein stability showed a linear correlation between bentonite requirement and the concentration of chitinases in wine, indicating the importance of removal of chitinases to achieve protein stabilization. The findings presented here contribute to an improved understanding of the variable concentration of haze-forming PR proteins in juice as affected by grape harvesting and processing conditions, and hence the variation in bentonite requirement for resultant wine.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Article

Authors

Bin Tian*, Claire Grose, James Morton, Marlene Jaspers, Mike Trought, Roland Harrison

*Lincoln University

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Effect of nanofiltration on the chemical composition and wine quality

In Enology the conventional processes of filtration for clarification and stabilization are giving place to alternative membrane processes, including nanofiltration (NF). Furthermore, the increased alcohol content in wines recorded in recent years became an important issue for all the main wine producing countries. Among techniques available to the wine industry to reduce the ethanol content, NF is certainly one of the newest. This study is focused on the evaluation of NF influence on wine physical-chemical composition, including mineral content, which in accordance to our best knowledge is a novelty.

Chemical markers in wine related to low levels of yeast available nitrogen in the grape

Nitrogen is an important nutrient of yeast and its low content in grape must is a major cause for sluggish fermentations. To prevent problems during fermentation, a supplementation of the must with ammonium salts or more complex nitrogen mixtures is practiced in the cellar. However this correction seems to improve only partially the quality of wine [1]. In fact, yeast is using nitrogen in many of its metabolic pathways and depending of the sort of the nitrogen source (ammonium or amino acids) it produces different flavor active compounds. A limitation in amino acids can lead to a change in the metabolic pathways of yeast and consequently alter wine quality.

Sensory definition of green aroma concept in red French wines. Evidence for the contribution of novel volatile markers

The aromatic complexity of a wine results from the perception of the association of volatile molecules and each aroma can be categorized into different families. The “green” aromas family in red wines has retained our attention by its close link with the fruity perception. In that study, the “green” olfactory concept of red wines was considered through a strategy combining both sensory analysis and hyphenated chromatographic techniques including HPLC and MDGC (Multidimensional Gas Chromatography). The aromatic space of this concept was specified by lexical generation through a free association task on 22 selected wines by a panel of wine experts. Then, 70 French red wines were scored on the basis of the intensity of their “green” and “fruity” attributes.

Prevention of wine oxidation during barrel aging: an innovative method to measure antioxidant

Wine oxidation is a problem that affects the freshness, the aromatic profile, the colour and also the mouthfeel of the wine. It mainly concerns white wines. Oxygen interactions with wine compounds lead to the phenomena cited above that are responsible for the depreciation of these wines. Barrel aging is a crucial step in the wine process because it allows many modifications as wine enrichment, colour stabilization, clarification and also a slow oxygenation of the wine. Effects of the oak barrel have to be known to prevent oxidation of the wine. We have been interested in the main antioxidant compounds released by oak barrels to the wine and we have developed an innovative method to reach directly these antioxidant compounds at the oak stave surface.

Molecular cloning and characterization of UDP-glucose: furaneol glucosyltransferase gene from Japanese

2,5-Dimethyl-4-hydroxy-3(2H)-furanone (furaneol) is an important aroma compound in fruits, such as pineapple and strawberry, and is reported to contribute to the strawberry-like note in some wines. Several grapevine species are used in winemaking, and furaneol is one of the characteristic aroma compounds in wines made from American grape (Vitis labrusca) and its hybrid grape, similar to methyl anthranilate. Muscat Bailey A is a hybrid grape variety [V. labrusca (Bailey) x V. vinifera (Muscat Hamburg)], and its wine is one of the most popular in Japan. The inclusion of Muscat Bailey A in the ‘International List of Vine and Varieties and their Synonyms’ managed by the ‘International Organisation of Vine and Wine (OIV)’ in 2013 has further fueled its popularity among winemakers and researchers worldwide.