Macrowine 2021
IVES 9 IVES Conference Series 9 Extraction of pathogenesis-related proteins and phenolics in Sauvignon Blanc as affected by different

Extraction of pathogenesis-related proteins and phenolics in Sauvignon Blanc as affected by different

Abstract

The composition of wine is largely determined by the composition of pre-fermentation juice, which is influenced by extraction of grape components. Different grape harvesting and processing conditions could affect the extraction of grape components into juice. Among these grape components, pathogenesis-related (PR) proteins are of great concern for white wine maker as they are the main cause of haze formation in finished white wine. If not removed before bottling, these PR proteins may progress into haze through the formation of complex with phenolics under certain conditions. Thaumatin-like proteins (TLPs) and chitinases are the main constituents of PR proteins found in protein haze. The aim of this study was to investigate the effects of grape harvesting and processing conditions on extraction of haze-forming PR proteins and phenolics, and the consequent impact on protein stability of wine. Sauvignon Blanc grapes were harvested by hand and by machine in 2011 and 2012 in Marlborough, New Zealand. Hand harvested grapes were processed by two different treatments: whole bunch pressing without skin contact, and pressing after destemming and crushing followed by 3 h skin contact; machine harvested grapes were processed by pressing after destemming and crushing followed by 3 h skin contact. For each treatment, the juice pressing procedure was carried out by gradually increasing the pressing pressure to three different levels (0.4 MPa, 0.8 MPa and 1.6 MPa), and juice samples were collected at each pressure point and bottle-fermented separately. Results of this study showed that pressing after destemming and crushing followed by 3 h skin contact can result in greater extraction of proteins and phenolics from grapes into juice compared with whole bunch pressing. Juice and wine obtained from machine harvested grapes showed a tendency of having lower concentration of proteins, as well as, PR proteins. Juice and wine obtained from higher pressing pressure showed the highest concentration of phenolics but the lowest concentration of proteins, suggesting that proteins in grapes are easily extracted at low pressing pressure, but greater extraction of phenolics in grapes requires higher pressing pressure. Analysis of wine protein stability showed a linear correlation between bentonite requirement and the concentration of chitinases in wine, indicating the importance of removal of chitinases to achieve protein stabilization. The findings presented here contribute to an improved understanding of the variable concentration of haze-forming PR proteins in juice as affected by grape harvesting and processing conditions, and hence the variation in bentonite requirement for resultant wine.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Article

Authors

Bin Tian*, Claire Grose, James Morton, Marlene Jaspers, Mike Trought, Roland Harrison

*Lincoln University

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Foam characteristics of white, rosé and red sparkling wines elaborated by the champenoise method

Contribution Foam is the characteristic that differentiates sparkling wines from still wines, being the first sensory attribute that tasters and consumers perceive and that determines the final quality of sparkling wines [1]. The foaming properties mainly depend on the chemical composition of wines [2-3], and different factors involved in wine composition will have an effect on foam quality. In Spain, the sparkling wine market focuses on the production of white and rosé sparkling wine, with very low production of red sparkling wines. However, this type of wines is elaborated in countries like Australia, South-Africa, Argentina, Italy or Portugal, with a great acceptance by consumers. No studies on the foaming characteristics of red sparkling wines have been found.

Characterization of Glycosidically Bound Aroma Compounds of País cv. grapes of different Chilean zones

País grape has been estimated to arrive to Chile almost 500 years ago, being the first strain grown in this country. Traditionally, this grape has been used to mix with other varieties, to produce poor quality wines, but today is beginning to be used in the production of high quality wines. However, very little is known about the chemical characteristics of this variety. The aroma is one of the most important quality attributes of wine. Volatile compounds of this beverage may come from the grape (varietal aromas), from the fermentation process, from the ageing. The aromatic compounds are found in the grape in two forms: as free volatile compounds and as non-volatile compounds. The last ones, are aroma precursors present mainly as glycoconjugates formed by a sugar and an aglycone…

An excessive leaf-fruit ratio reduces the yeast assimilable nitrogen in the must

Yeast assimilable nitrogen (YAN) in the grape must is a key variable for wine quality as a source of aroma precursors. In a situation of YAN deficiency, a foliar urea application upon the vine at veraison enhances YAN concentration and facilitates must fermentation. In 2013, Agroscope investigated the impact of leaf-fruit ratio on the nitrogen (N) assimilation and partitioning in grapevine Vitis vinifera cv. Chasselas following foliar-urea application with the aim of improving its efficiency on the YAN concentration.

Effects of post-fermentative cold maceration on chemical and sensory characteristics of Syrah, Cabernet Franc and Montepulciano wines

Astringency sensation decreases slowly during the aging of red wine. Complex reactions of condensation and precipitation of wine polyphenols are involved in this phenomenon. Wine composition and conditions of aging, such as temperature and oxygen availability, strongly influence evolution of the phenol matrix. Recently, a Post-Fermentative cold Maceration (PFM) technique was tested with the aim of accelerating reactions leading to the reduction of astringency and exploiting chemical compounds not extracted from the solid parts of grapes during the previous traditional maceration phase. To this purpose, an innovative maceration system was engineered and used to perform PFM trials on marc derived from vinification of different varieties of red grapes.

Comprehensive two-dimensional gas chromatography coupled with Tof-MS, a powerful tool for analysis of the volatomes of grapes and wines

Comprehensive two-dimensional gas chromatography (GCxGC) has emerged as a powerful analytical technique for unraveling the volatile composition of complex matrices. This work will present three applications of GCxGC Tof-MS to the oenological field, aimed to identify novel biomarkers to be used in the quality control process of the wine industry. Comprehensive mapping of volatile compounds was conducted in a large sample of 70 sparkling wines, produced by 48 different wineries across 6 vintages and representative of the two main production areas for premium Italian sparkling wines (Franciacorta (FC) and Trentodoc (TN)), using HS-SPME followed by GCxGC-Tof-MS and multivariate analysis. Selection and identification of 196 putative biomarkers allowed clear separation of sparkling wines from FC and TN.