Macrowine 2021
IVES 9 IVES Conference Series 9 Extraction of pathogenesis-related proteins and phenolics in Sauvignon Blanc as affected by different

Extraction of pathogenesis-related proteins and phenolics in Sauvignon Blanc as affected by different

Abstract

The composition of wine is largely determined by the composition of pre-fermentation juice, which is influenced by extraction of grape components. Different grape harvesting and processing conditions could affect the extraction of grape components into juice. Among these grape components, pathogenesis-related (PR) proteins are of great concern for white wine maker as they are the main cause of haze formation in finished white wine. If not removed before bottling, these PR proteins may progress into haze through the formation of complex with phenolics under certain conditions. Thaumatin-like proteins (TLPs) and chitinases are the main constituents of PR proteins found in protein haze. The aim of this study was to investigate the effects of grape harvesting and processing conditions on extraction of haze-forming PR proteins and phenolics, and the consequent impact on protein stability of wine. Sauvignon Blanc grapes were harvested by hand and by machine in 2011 and 2012 in Marlborough, New Zealand. Hand harvested grapes were processed by two different treatments: whole bunch pressing without skin contact, and pressing after destemming and crushing followed by 3 h skin contact; machine harvested grapes were processed by pressing after destemming and crushing followed by 3 h skin contact. For each treatment, the juice pressing procedure was carried out by gradually increasing the pressing pressure to three different levels (0.4 MPa, 0.8 MPa and 1.6 MPa), and juice samples were collected at each pressure point and bottle-fermented separately. Results of this study showed that pressing after destemming and crushing followed by 3 h skin contact can result in greater extraction of proteins and phenolics from grapes into juice compared with whole bunch pressing. Juice and wine obtained from machine harvested grapes showed a tendency of having lower concentration of proteins, as well as, PR proteins. Juice and wine obtained from higher pressing pressure showed the highest concentration of phenolics but the lowest concentration of proteins, suggesting that proteins in grapes are easily extracted at low pressing pressure, but greater extraction of phenolics in grapes requires higher pressing pressure. Analysis of wine protein stability showed a linear correlation between bentonite requirement and the concentration of chitinases in wine, indicating the importance of removal of chitinases to achieve protein stabilization. The findings presented here contribute to an improved understanding of the variable concentration of haze-forming PR proteins in juice as affected by grape harvesting and processing conditions, and hence the variation in bentonite requirement for resultant wine.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Article

Authors

Bin Tian*, Claire Grose, James Morton, Marlene Jaspers, Mike Trought, Roland Harrison

*Lincoln University

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Effect of different foliar nitrogen applications on the must amino acids and glutathione composition in Cabernet Sauvignon vineyard

Cabernet Sauvignon is one of the most important winegrape varieties in Chile. However, temperature raise and decreased rainfall due to climate change can lead to grape quality decrease in certain areas. Amino acids are essential as nitrogen source for yeast but also directly affect grape quality serving as precursors of certain volatile compounds that enhance the wine bouquet. Besides, glutathione is an important tripeptide acting as antioxidant, preventing the appearance of browning pigments in must and exerts a protective effect in volatile compounds.

Effect of intra‐vineyard ripeness variation on the efficiency of commercial enzymes on berry cell wall deconstruction under winemaking conditions

Intra-vineyard variation grape berry ripening occurs within bunches, between bunches on the same vine and between vines. Although it is assumed that such variation also occurs at the grape berry cell wall level, no study to data has investigated in any depth. Here we have used a intra-vineyard panel design to investigate pooled bunches from six vines (per panel) in the context of a winemaking scenario. The dissected vineyard was harvested by separate panels, where each panel was then subjected to a standard winemaking procedure with or without the addition of three different enzyme preparations for maceration.

Influence of SO2 and Zinc on the formation of volatile aldehydes during alcoholic fermentation

Laboratório de Análisis del Aroma y Enologia (LAAE). Department of Analytical Chemistry, Faculty of Sciences, Universidad de Zaragoza, 50009, Zaragoza, Spain, During alcoholic fermentation, fusel (or Strecker) aldehydes are intermediates in the amino acid catabolism to form fusel alcohols following the Ehrlich Pathway (1). One of the main enzymes involved in this pathway is Alcohol Dehydrogenase (ADH), whose activity is highly strain dependent and determines the rate of conversion of aldehydes into fusel alcohols (2). This enzyme has a Zn2+ catalytic binding site, which suggests that the must Zn2+ levels will most likely influence the rate of reduction of aldehydes into alcohols. On the other hand, SO2 is commonly used in winemaking for its antiseptic and antioxidant properties.

The effect of cropload on the volatile aroma characteristics of ‘Beihong’ and ‘Beimei’ red wine

Beihong and Beimei were bred as winemaking cultivars released by Institute of Botany, the Chinese Academy of Sciences in 2008. The cultivars are selected from the population of ‘Muscat Hamburg’ (Vitis vinifera) ×V. amurensis. They are extended to most provinces in North of China because they have strong resistance to cold and disease and need not be buried in soil in winter. To better understand the effect of cropload on volatile compounds during wine-making, we surveyed volatiles composition and content of different cropload level in 3-years-old ‘Beihong’ and ‘Beimei’ vines which planted in east foot of Helan mountain of Ningxia (EHN).

A combination of biotechnology tools and coopers elements for an alternative the addition of SO2 at the end of the malolactic fermentation in red wines or at the “mutage” for the “liquoreux” wines

In red wines the post-MLF SO2 addition is an essential event. It is also the case for the “mutage” during the elaboration of the “liquoreux”. At these moments SO2 plays an antimicrobial action and an antioxidant effect. But at current pH of wines, ensuring a powerful molecular SO2 has become very difficult. Recent work on Brettanomyces strains have also shown that some strains are resistant up to 1.2 mg / L of molecular SO2. It’s also the case of the some Saccharomuces or Zygosaccharomyces strains suitable to re-ferment “liquoreux” wines after the “mutage”.