Macrowine 2021
IVES 9 IVES Conference Series 9 Chemical markers in wine related to low levels of yeast available nitrogen in the grape

Chemical markers in wine related to low levels of yeast available nitrogen in the grape

Abstract

Nitrogen is an important nutrient of yeast and its low content in grape must is a major cause for sluggish fermentations. To prevent problems during fermentation, a supplementation of the must with ammonium salts or more complex nitrogen mixtures is practiced in the cellar. However this correction seems to improve only partially the quality of wine [1]. In fact, yeast is using nitrogen in many of its metabolic pathways and depending of the sort of the nitrogen source (ammonium or amino acids) it produces different flavor active compounds. A limitation in amino acids can lead to a change in the metabolic pathways of yeast and consequently alter wine quality. A better management of nitrogen fertilization of vineyards can significantly increase the quantity of yeast available nitrogen (YAN) in the grape and consequently the wine quality. A metabolomics study comparing Chasselas and Doral wines produced from grapes of two variants, nitrogen deficient culture versus supplemented with foliar urea, indicated several markers related to nitrogen supplementation of grapes [2]. Four substances from these potential markers were chosen for the present study: 2-phenylethanol, 2- plus 3-methylbutanol, succinic acid and prolin. The production of these compounds is known to be influenced by the nitrogen content of the must and they are in easily measurable concentrations in the wine. The objective of this work is to study the correlation between the concentration of YAN in must and the concentration of the four potential chemical markers in the wine using a significant number of samples ( > 130) including different grape varieties (13), harvests (2009-2014) and yeast types. The goal is to create a model for the retro-prediction of YAN concentration in the original must based on the chemical analysis of the wine. This information can be used by winemakers to optimize nitrogen fertilization of their vineyards. Wines produced in the cellar of AGROSCOPE using a standard protocol without addition of ammonium salt were used for this study. The concentration of YAN in the must was determined before the fermentation. The concentrations of the potential markers in the wine were measured for: the higher alcohols (2-phenylethanol, 2- and 3-methylbutanol) by GC-FID, for succinic acid by enzymatic method and for prolin by HPLC-MS. The four markers show a good correlation (R2 > 0.7) with YAN content in must. Grape variety and yeast variety strongly influence these correlations whereas harvest is less important. The distribution of YAN between ammonium and different individual amino acids explains the major part of the effect from the grape varieties. Reference 1. Bréant, L., Marti, G., Dienes-Nagy, Á., Zufferey, V., Rösti, J., Lorenzini, F., Gindro, K., Viret, O., Wolfender, J-L., In Vino Analytica Scientia 2013, Abstract Book, Poster 80 2. Lorenzini, F.; Vuichard, F.; Revue Suisse de Viticulture Arboriculture Horticulture, 44 (2012), pp 96-103

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Agnes Dienes-Nagy*, Carole Koestel, Fabrice Lorenzini, Johannes Rösti

*AGROSCOPE

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Study of the content of amino acids and biogenic amines in sparkling red wines

The production of red sparkling wines is lower in Spain in comparison with the winemaking of white or rosé sparkling wines. In red sparkling wine processing it is essential to obtain suitable base wines that should have moderate alcohol content, high acidity, good color values, an adequate mouth-feel and a sweet tannin. Grapes for sparkling wine production have to be harvested at low maturity stages, with lower alcohol contents and higher acidities, which will that the phenolic maturity of the grapes is also low, showing green tannins. This paper analyses different treatments in order to minimize these inconveniences: cold maceration-prefermentation and delestage to elaborate the grapes with lower maturity, must nanofiltration, and the partial osmosis of the wines made from grapes with an adequate maturity degree.

The impact of branched chain and aromatic amino acids on fermentation kinetics and aroma biosynthesis by wine yeast Saccharomyces cerevisiae

One of the major determinants of wine quality is the aroma. Wine aroma is the human perception of the matrix of grape and yeast derived volatiles and their interaction that contribute to flavour wine. Most common are higher alcohols, ester and aldehydes. In previous studies the formation of characteristic volatile compounds have been linked to the metabolism of branched-chain and aromatic amino acids
(BCAAs) in synthetic grape must. Here we report on an investigation to assess the impact of the initial amino acid concentration on the production of aroma compounds by the industrial yeast VIN13 grown in both synthetic and real grape musts.

Study of the colour and phenolic evolution of three different tannin/anthocyanin ratios over time in a model wine

Phenolic compounds are important quality indicators in red wine. A large number of polyphenols play an important role in wine development, contributing to the colour and the sensory perception of the wines. Anthocyanins are the pigments responsible for the colour in young red wines while tannins are the principal contributors to the bitterness and the astringency of the wines. Wine polyphenols are considered more complex molecules than grape phenolics, due to the enormous number of chemical reactions which take place during the entire winemaking process and storage, forming more stable compounds.

Bentonite fining in cold wines: prediction tests, reduced efficiency and possibilities to avoid additional fining treatments

Bentonite fining is widely used to prevent protein haze in white wines. Most wineries use laboratory-scale fining trials to define the appropriate amount of bentonite to be used in the cellar. Those pre-tests need to mimic as much as possible the industrial scale fining procedure to determine the exact amount of bentonite necessary for protein stability. Nevertheless it is frequent that, after fining with the recommended amount of bentonite, wines appear still unstable and need an additional fining treatment. It remains a major challenge to understand why the same wine, fined with the same dosage of the same bentonite, achieves stability in the lab, but not in the cellar.

Characterization of Glycosidically Bound Aroma Compounds of País cv. grapes of different Chilean zones

País grape has been estimated to arrive to Chile almost 500 years ago, being the first strain grown in this country. Traditionally, this grape has been used to mix with other varieties, to produce poor quality wines, but today is beginning to be used in the production of high quality wines. However, very little is known about the chemical characteristics of this variety. The aroma is one of the most important quality attributes of wine. Volatile compounds of this beverage may come from the grape (varietal aromas), from the fermentation process, from the ageing. The aromatic compounds are found in the grape in two forms: as free volatile compounds and as non-volatile compounds. The last ones, are aroma precursors present mainly as glycoconjugates formed by a sugar and an aglycone…