Macrowine 2021
IVES 9 IVES Conference Series 9 Chemical markers in wine related to low levels of yeast available nitrogen in the grape

Chemical markers in wine related to low levels of yeast available nitrogen in the grape

Abstract

Nitrogen is an important nutrient of yeast and its low content in grape must is a major cause for sluggish fermentations. To prevent problems during fermentation, a supplementation of the must with ammonium salts or more complex nitrogen mixtures is practiced in the cellar. However this correction seems to improve only partially the quality of wine [1]. In fact, yeast is using nitrogen in many of its metabolic pathways and depending of the sort of the nitrogen source (ammonium or amino acids) it produces different flavor active compounds. A limitation in amino acids can lead to a change in the metabolic pathways of yeast and consequently alter wine quality. A better management of nitrogen fertilization of vineyards can significantly increase the quantity of yeast available nitrogen (YAN) in the grape and consequently the wine quality. A metabolomics study comparing Chasselas and Doral wines produced from grapes of two variants, nitrogen deficient culture versus supplemented with foliar urea, indicated several markers related to nitrogen supplementation of grapes [2]. Four substances from these potential markers were chosen for the present study: 2-phenylethanol, 2- plus 3-methylbutanol, succinic acid and prolin. The production of these compounds is known to be influenced by the nitrogen content of the must and they are in easily measurable concentrations in the wine. The objective of this work is to study the correlation between the concentration of YAN in must and the concentration of the four potential chemical markers in the wine using a significant number of samples ( > 130) including different grape varieties (13), harvests (2009-2014) and yeast types. The goal is to create a model for the retro-prediction of YAN concentration in the original must based on the chemical analysis of the wine. This information can be used by winemakers to optimize nitrogen fertilization of their vineyards. Wines produced in the cellar of AGROSCOPE using a standard protocol without addition of ammonium salt were used for this study. The concentration of YAN in the must was determined before the fermentation. The concentrations of the potential markers in the wine were measured for: the higher alcohols (2-phenylethanol, 2- and 3-methylbutanol) by GC-FID, for succinic acid by enzymatic method and for prolin by HPLC-MS. The four markers show a good correlation (R2 > 0.7) with YAN content in must. Grape variety and yeast variety strongly influence these correlations whereas harvest is less important. The distribution of YAN between ammonium and different individual amino acids explains the major part of the effect from the grape varieties. Reference 1. Bréant, L., Marti, G., Dienes-Nagy, Á., Zufferey, V., Rösti, J., Lorenzini, F., Gindro, K., Viret, O., Wolfender, J-L., In Vino Analytica Scientia 2013, Abstract Book, Poster 80 2. Lorenzini, F.; Vuichard, F.; Revue Suisse de Viticulture Arboriculture Horticulture, 44 (2012), pp 96-103

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Agnes Dienes-Nagy*, Carole Koestel, Fabrice Lorenzini, Johannes Rösti

*AGROSCOPE

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Oenological features of Sangiovese wine from vinification of whole grape berries

The present study was performed in a traditional winery located in the viticultural area of Brunello di Montalcino, Siena, Italy, in the vintage 2015. Actually, in this winery Sangiovese grape musts are fermented in large oak barrels by a single strain of Saccharomyces cerevisiae previously isolated in the same winery. Pumping over operations are carried out once or twice a day until the end of alcoholic fermentations. The aim of this work was to investigate on the oenological properties of Sangiovese wine produced with the traditional winemaking process adopted by the winery under study obtained from the fermentation of whole berries compared to that from crushed grape must. In particular, two lots of 65q of Sangiovese grapes from the same 3ha vineyard were vinified in 150hL oak barrels.

Characterization of free and glycosidically bound simple phenols in hybrid grape varieties using liquid chromatography coupled to high resolution mass (q-orbitrap)

Vitis vinifera is one of the most diffused grapevines over the word and it is the raw material for high quality wines production. The availability of more resistant interspecific hybrid vine varieties, developed from crosses between Vitis vinifera and other Vitis species, has generating much interest, also due to the low environmental effect of production. However, hybrid grape wine composition and varietal differences between interspecific hybrids are not well defined. Different studies revealed that wine consumption has health effects due to its high content of antioxidants, as phenolic compounds. In particular, simple phenols are appreciated not only for their physiological health benefits, including antioxidant, anti-inflammatory and cardioprotective effects, but also because they affect wines organoleptic profile and have a significant role in defining their nutritional characteristics.

Effect of ageing with Specific Inactivated Dry Yeasts on the volatile composition of Sauvignon Blanc and Carménère wines

Úbeda-Aguilera, C a, b, Peña-Neira, A.b Del Barrio-Galán, R.b, c a Biomedical Sciences Institute, Science Faculty, Universidad Autónoma de Chile, Chile. b Department of Agro-Industry and Enology, Faculty of Agronomical Sciences, University of Chile, Post Office Box 1004, Santa Rosa 11315, La Pintana, Santiago, Chile c Lallemand Inc. Chile y Compañía Limitada, Rosario Norte 407, piso 6, Las Condes, Santiago, Chile The wine is a complex matrix made up of several compounds which can interact among themselves throughout the wine ageing process, thereby modifying their sensorial characteristics. It is well known that during ageing of wines on lees, polysaccharides (mainly mannoproteins) can be released and can interact with the aromatic fraction modifying its volatility.

Microbial life in the grapevine: what can we expect from the leaf microbiome?

The above-ground parts of plants, which constitute the phyllosphere, have long been considered devoid of bacteria and fungi, at least in their internal tissues and microbial presence there was long considered a sign of disease. However, recent studies have shown that plants harbour complex bacterial communities, the so-called “microbiome”[1]. We are only beginning to unravel the origin of these bacterial plant inhabitants, their community structure and their roles, which in analogy to the gut microbiome, are likely to be of essential nature. Among their multifaceted metabolic possibilities, bacteria have been recently demonstrated to emit a wide range of volatile organic compounds (VOCs), which can greatly impact the growth and development of both the plant and its disease-causing agents.

Analysis of the oenological potentials of different oak forests in Hungary

Like France, Hungary has many oak forests used for making barrels since many years. But if the differences between the woods of the North, the East and the South-West forests of France are well known, this is probably not the case of Hungarian forests. However taking into account the essential differences of climates and soils, differences must be significant and the general name “Hungarian oak” must not have any real meaning. We have studied precisely (determination of concentrations of volatile and non-volatile wood compounds, anatomical criteria, measurement of antioxidant capacity) of oaks collected from northeastern Hungary and others collected from the Danube valley in the northwest of the country.