Macrowine 2021
IVES 9 IVES Conference Series 9 Chemical markers in wine related to low levels of yeast available nitrogen in the grape

Chemical markers in wine related to low levels of yeast available nitrogen in the grape

Abstract

Nitrogen is an important nutrient of yeast and its low content in grape must is a major cause for sluggish fermentations. To prevent problems during fermentation, a supplementation of the must with ammonium salts or more complex nitrogen mixtures is practiced in the cellar. However this correction seems to improve only partially the quality of wine [1]. In fact, yeast is using nitrogen in many of its metabolic pathways and depending of the sort of the nitrogen source (ammonium or amino acids) it produces different flavor active compounds. A limitation in amino acids can lead to a change in the metabolic pathways of yeast and consequently alter wine quality. A better management of nitrogen fertilization of vineyards can significantly increase the quantity of yeast available nitrogen (YAN) in the grape and consequently the wine quality. A metabolomics study comparing Chasselas and Doral wines produced from grapes of two variants, nitrogen deficient culture versus supplemented with foliar urea, indicated several markers related to nitrogen supplementation of grapes [2]. Four substances from these potential markers were chosen for the present study: 2-phenylethanol, 2- plus 3-methylbutanol, succinic acid and prolin. The production of these compounds is known to be influenced by the nitrogen content of the must and they are in easily measurable concentrations in the wine. The objective of this work is to study the correlation between the concentration of YAN in must and the concentration of the four potential chemical markers in the wine using a significant number of samples ( > 130) including different grape varieties (13), harvests (2009-2014) and yeast types. The goal is to create a model for the retro-prediction of YAN concentration in the original must based on the chemical analysis of the wine. This information can be used by winemakers to optimize nitrogen fertilization of their vineyards. Wines produced in the cellar of AGROSCOPE using a standard protocol without addition of ammonium salt were used for this study. The concentration of YAN in the must was determined before the fermentation. The concentrations of the potential markers in the wine were measured for: the higher alcohols (2-phenylethanol, 2- and 3-methylbutanol) by GC-FID, for succinic acid by enzymatic method and for prolin by HPLC-MS. The four markers show a good correlation (R2 > 0.7) with YAN content in must. Grape variety and yeast variety strongly influence these correlations whereas harvest is less important. The distribution of YAN between ammonium and different individual amino acids explains the major part of the effect from the grape varieties. Reference 1. Bréant, L., Marti, G., Dienes-Nagy, Á., Zufferey, V., Rösti, J., Lorenzini, F., Gindro, K., Viret, O., Wolfender, J-L., In Vino Analytica Scientia 2013, Abstract Book, Poster 80 2. Lorenzini, F.; Vuichard, F.; Revue Suisse de Viticulture Arboriculture Horticulture, 44 (2012), pp 96-103

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Agnes Dienes-Nagy*, Carole Koestel, Fabrice Lorenzini, Johannes Rösti

*AGROSCOPE

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Characterization of free and glycosidically bound simple phenols in hybrid grape varieties using liquid chromatography coupled to high resolution mass (q-orbitrap)

Vitis vinifera is one of the most diffused grapevines over the word and it is the raw material for high quality wines production. The availability of more resistant interspecific hybrid vine varieties, developed from crosses between Vitis vinifera and other Vitis species, has generating much interest, also due to the low environmental effect of production. However, hybrid grape wine composition and varietal differences between interspecific hybrids are not well defined. Different studies revealed that wine consumption has health effects due to its high content of antioxidants, as phenolic compounds. In particular, simple phenols are appreciated not only for their physiological health benefits, including antioxidant, anti-inflammatory and cardioprotective effects, but also because they affect wines organoleptic profile and have a significant role in defining their nutritional characteristics.

Maturation of Agiorgitiko (Vitis vinifera) red wine on its wine lees: Impact on its phenolic composition

Maturation of wine on lees (often referred as sur lie) is a common practice applied by many winemakers around the world. In the past this method was applied mainly on white and/or sparkling wine production but recently also to red wine production. In our experiment, we matured red wine on wine lees of two origins: a) Light wine lees, collected after the completion of the alcoholic fermentation, b) Heavy lees, collected after the completion of the malolactic fermentation. The lees were free of off-odors and were added in the red wine in percentage 3% and 8%, simulating common winemaking addition. The maturation lasted in total six months and samples were collected for analysis after one, three and six months. During storage the lees were stirred.

Use of chitosan as a secondary antioxidant in juices and wines

Chitosan is a polysaccharide produced from the deacetylation of chitin extracted from crustaceous and fungi. In winemaking chitosan is mainly used in the clarification of grape juice and wine, stabilization of white wines, removal of metals and to prevent wine spoilage by undesired microorganisms. The addition of chitosan to model wine systems was able to retard browning, reduce levels of metallic ions (Fe and Cu) and to protect varietal thiols due to its antiradical activity1. The present experiment was planned in order to evaluate the use of chitosan as a secondary antioxidant at three different stages of Sauvignon blanc fermentation and winemaking. Sauvignon blanc juices from three different locations were obtained at a commercial winery in Marlborough, New Zealand. One lots of grapes was collected from a receival bin and pressed into juice with a water-bag press, and a further juice sample was collected from a commercial pressing operation. Chitosan (1 g/L, low molecular weight, 75 – 85% deacetylated) was added to the juice after pressing, after cold settling, after fermentation, or at all these stages. Controls without any chitosan additions were also prepared.

Supramolecular approaches to the study of the astringency elicited by wine phenolic compounds

The objective of this study is to review the scientific evidences and to advance into the knowledge of the molecular mechanisms of astringency. Astringency has been described as the drying, roughing and puckering sensation perceived when some food and beverages are tasted (1). The main, but possibly not the only, mechanism for the astringency is the precipitation of salivary proteins (2,3). Between phenolic compounds found in red wines, flavan-3-ols are the group usually related to the development of this sensation. Other compounds, phenolic or not, like anthocyanins, polysaccharides and mannoproteins could act modifying or modulating astringency perception by hindering the interaction between flavanols and salivary proteins either because of their interaction with the flavanols or because of their interaction with the salivary proteins.

Interest and impact of PVP/PVI (Polyvinylpyrrolidone/ Polyvinylimidazole) on winemaking and final quality of wines

Céline Sparrow a, Christophe Morge a, a SOFRALAB SAS, 79, av. A.A. Thévenet – CS 11031 – 51530 Magenta, France Consumers’ health and security force authorities to limit, in wine as in others food industry products, the concentration in « dangerous » molecules. Therefore the legal limit in heavy metals keeps on decreasing. As per proof EU regulation just decrease the stain concentration in wine from 0,2 to 0,15 mg/l. Certain changes , such as sodium arsenite treatment in vines, disappearance of brass in wineries to the benefit of stainless steel, limit even more the concentration of heavy metals in wines. But the use of copper derivates in vines treatments is difficult to replace. In the case of wine and its elaboration, the problem is even more complex. Indeed, regulation forces the wine producers to control the concentration of certain heavy metals in final wines.