Macrowine 2021
IVES 9 IVES Conference Series 9 Chemical markers in wine related to low levels of yeast available nitrogen in the grape

Chemical markers in wine related to low levels of yeast available nitrogen in the grape

Abstract

Nitrogen is an important nutrient of yeast and its low content in grape must is a major cause for sluggish fermentations. To prevent problems during fermentation, a supplementation of the must with ammonium salts or more complex nitrogen mixtures is practiced in the cellar. However this correction seems to improve only partially the quality of wine [1]. In fact, yeast is using nitrogen in many of its metabolic pathways and depending of the sort of the nitrogen source (ammonium or amino acids) it produces different flavor active compounds. A limitation in amino acids can lead to a change in the metabolic pathways of yeast and consequently alter wine quality. A better management of nitrogen fertilization of vineyards can significantly increase the quantity of yeast available nitrogen (YAN) in the grape and consequently the wine quality. A metabolomics study comparing Chasselas and Doral wines produced from grapes of two variants, nitrogen deficient culture versus supplemented with foliar urea, indicated several markers related to nitrogen supplementation of grapes [2]. Four substances from these potential markers were chosen for the present study: 2-phenylethanol, 2- plus 3-methylbutanol, succinic acid and prolin. The production of these compounds is known to be influenced by the nitrogen content of the must and they are in easily measurable concentrations in the wine. The objective of this work is to study the correlation between the concentration of YAN in must and the concentration of the four potential chemical markers in the wine using a significant number of samples ( > 130) including different grape varieties (13), harvests (2009-2014) and yeast types. The goal is to create a model for the retro-prediction of YAN concentration in the original must based on the chemical analysis of the wine. This information can be used by winemakers to optimize nitrogen fertilization of their vineyards. Wines produced in the cellar of AGROSCOPE using a standard protocol without addition of ammonium salt were used for this study. The concentration of YAN in the must was determined before the fermentation. The concentrations of the potential markers in the wine were measured for: the higher alcohols (2-phenylethanol, 2- and 3-methylbutanol) by GC-FID, for succinic acid by enzymatic method and for prolin by HPLC-MS. The four markers show a good correlation (R2 > 0.7) with YAN content in must. Grape variety and yeast variety strongly influence these correlations whereas harvest is less important. The distribution of YAN between ammonium and different individual amino acids explains the major part of the effect from the grape varieties. Reference 1. Bréant, L., Marti, G., Dienes-Nagy, Á., Zufferey, V., Rösti, J., Lorenzini, F., Gindro, K., Viret, O., Wolfender, J-L., In Vino Analytica Scientia 2013, Abstract Book, Poster 80 2. Lorenzini, F.; Vuichard, F.; Revue Suisse de Viticulture Arboriculture Horticulture, 44 (2012), pp 96-103

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Agnes Dienes-Nagy*, Carole Koestel, Fabrice Lorenzini, Johannes Rösti

*AGROSCOPE

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Analysis of peptide fraction from white wines

Among nitrogen compounds included in white wines, the peptide fraction is certainly the least studied, however this fraction is quantitatively the most important (Feuillat, 1974). Existing studies concern the fraction below 1 kDa and only for white and sparkling wines (Bartolomé et al, 1997, Desportes et al 2000). In this report, we have developed methods to isolate peptides from reference white wines. Then, we have applied this methodology with bitter wine to answer a research question: is there a relation between peptides and the bitterness of white wine as for some cheese for example (Furtado, 1984)?

Directed Evolution of Oenococcus oeni: optimising yeast-bacteria interactions for improved malolactic fermentation

Malolactic fermentation (MLF) is a secondary step in the vinification process and it follows alcoholic fermentation (AF) which is predominantly carried out by Saccharomyces cerevisiae. These two processes result in the degradation of metabolites to produce secondary metabolites which also contribute to the final wine flavour and quality. AF results in the production of ethanol and carbon dioxide from sugars and MLF stems from the degradation of L-malic acid (a dicarboxylic acid) to L-lactic acid (a monocarboxylic acid). The latter process results in a smoother texture as the acidity of the wine is reduced by the process, it also adds to the flavour complexity of the wine.

The moment of preharvest elicitor application influence its final effect on winegrapes quality

Phenolic compounds are secondary metabolites of grapes. Plants produce a wide variety of this type of metabolites through diverse biosynthesis pathways and their production is sometimes a response to external stimuli, either environmental or biotic stresses. Some of them may act as chemical defenses against pathogens or herbivores and their synthesis is increased when the attack exists. However, it is remarkable that the synthesis of these interesting compounds can be activated even when the stimulus is not present, with the use of elicitors. These are substances that when applied exogenously trigger the biosynthetic pathways conducting to the synthesis of these defense compounds.

Analysis of the oenological potentials of different oak forests in Hungary

Like France, Hungary has many oak forests used for making barrels since many years. But if the differences between the woods of the North, the East and the South-West forests of France are well known, this is probably not the case of Hungarian forests. However taking into account the essential differences of climates and soils, differences must be significant and the general name “Hungarian oak” must not have any real meaning. We have studied precisely (determination of concentrations of volatile and non-volatile wood compounds, anatomical criteria, measurement of antioxidant capacity) of oaks collected from northeastern Hungary and others collected from the Danube valley in the northwest of the country.

Study of the volatil profile of minority white varieties

The genetic material preservation is a priority issue in winemaking research. The recovery of minority grape varieties can control the genetic erosion, contributing also to preserve wine typical characteristics. In D.O.Ca. Rioja (Spain) the number of grown white varieties has been very limited, representing Viura the 91% of the cultivated white grape area in 2005, while the others, Garnacha Blanca and Malvasía riojana, hardly were grown. For this reason, a recovery and characterization study of plant material was carried out in this region. In 2008, the results obtained allowed the authorization of three minority white varieties: Tempranillo Blanco, Maturana Blanca and Turruntés.