Macrowine 2021
IVES 9 IVES Conference Series 9 Oligosaccharides in red wines: could their structure and composition be influenced by the grape-growing

Oligosaccharides in red wines: could their structure and composition be influenced by the grape-growing

Abstract

Oligosaccharides have only recently been characterized in wine, and the information on composition and content is still limited. In wine, these molecules are mainly natural byproducts of the degradation of grape berry cell wall polysaccharides. Wine oligosaccharides present several physicochemical properties, being one relevant factor linked to the astringency perception of wines (1,2). A terroir can be defined as a grouping of homogeneous environmental units based on the typicality of the products obtained. This notion is particularly associated with wine, being the climate and the soil two of the major elements of terroir concept. Monastrell red wines, predominant varietal wines from the Southern of Spain, were elaborated with grapes from four different terroirs: Cañada Judío, Albatana, Bullas and Montealegre. Climate and soil data from different terroirs were gathered to properly distinguish them. Oligosaccharide fractions from wines were isolated, after removal of phenolic compounds, by high resolution size-exclusion chromatography. The glycosyl–linkages composition was determined by GC–MS of the partially methylated alditol acetates. Results show differences in the glycosyl–linkages composition of oligosaccharides from wines, according to their terroir. The molar percentage of glucose, rhamnose, arabinose, xylose and mannose residues exhibit marked differences depending on the terroir. The ratio of the terminal to the branched residues for Cañada Judío, Albatana, Bullas and Montealegre oligosaccharides is, respectively, 0.75, 0.85, 0.99 and 0.89. Proportions of oligosaccharides families have been calculated from glycosyl-linkage data (3,4,5). Montealegre wine clearly presents the lowest relative molar percentage for the oligosaccharides from yeasts (the sum of OligoGlucans and OligoMannans) and also for OligoXyloGlucans, whereas Bullas wine exhibits by far the lowest release of OligoRhamnogalacturonans. OligoArabinans and OligoArabiGalactans type II also show differences according to the terroir. All these data were treated by PCA to permit a best understanding. The projections on the first axis show obvious separation of Montealegre, whereas a clear separation of Albatana is observed in the projections on the second axis. The first and second principal components represent, respectively, 69% and 19% of the variability for samples. In summary, our results suggest the impact of “terroir” on the structure and the composition of wine oligosaccharide fraction, which could affect their physicochemical and sensory properties.

1.Quijada-Morín et al. (2014). Food Chem. 154, 44–51. 2.Boulet et al. (2016). Food Chem. 190, 357–363 3.Ducasse et al. (2011). J Agric Food Chem. 59, 6558–6567. 4.Ballou (1982). In Strathern, Jones & Broach (Eds.), Metabolism and gene expression (335–360), NY. 5.Fry et al. (1983). Plant Physiol. 89, 1–3.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Rafael Apolinar-Valiente*, Encarna Gómez-Plaza, José María Ros-García, Pascale Williams, Thierry Doco

*INRA Montpellier

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Grape byproducts as source of resveratrol oligomers for the development of antifungal extracts

Grape canes are a non-recycled byproduct of wine industry (1-5 tons per hectare per year) containing valuable phytochemicals of medicine and agronomical interest. Resveratrol and wine polyphenols are known to exert a plethora of health-promoting effects including antioxidant capacity, cardioprotection, anticancer activity, anti-inflammatory effects, and estrogenic/antiestrogenic properties (Guerrero et al. 2009). Additionally, resveratrol is a major phytoalexin produced by plants in response to various stresses and promotes disease resistance (Chang et al. 2011). Our project aims to develop polyphenol-rich grape cane extracts to fight phytopathogenic or clinically relevant fungi. We initiate the project with the development of analytical methods to analyze resveratrol mono- and oligomers (dimers, trimers and tetramers) from grape canes and we evaluate their potential activity against clinically relevant opportunistic fungal pathogens (Houillé et al. 2014).

Correlations between N,S,O-heterocycle levels and age of Champagne base wines

Champagne regulation allows winegrowers to stock small amounts of still wines in order to compensate vintages’ quality shifts mainly due to climate variations. According to their technical requirements and house style some Champagne producers (commonly named “Champagne houses”) use these stored wines in the blend in order to introduce an element of complexity. These wines possess the particularity of being aged on fine lees in thermo-regulated stainless steel tanks. The Champagne house of Veuve Clicquot Ponsardin has several wines stored this way.

Impact of drought stress on concentration and composition of wine proteins in Riesling

Protein haze in white wines is a major technological and economic problem of the wine industry. Field tests were carried out in steep slope vineyards planted with Riesling grapes over 3 dry growing seasons to study the effect of drought stress on the concentration of proteins in the resulting wines. Plots suffering from drought stress were compared with surrounding drip irrigated plots. Riesling grapes were processed into wines by conventional procedures. Protein amounts of the isolated wine colloids of the stressed samples were always higher than those of the watered samples(mean watered 13.8 ± 0.44, mean stressed 17.4 ± 0.40 g 100 g-1). As a consequence, higher bentonite doses were needed to achieve protein haze stability of the drought stressed treatments.

Impact of smoke exposure on the chemical composition of grapes

Vineyard exposure to smoke can lead to grapes and wine which exhibit objectionable smoky and ashy aromas and flavours, more commonly known as ‘smoke taint’ [1, 2]. In the last decade, significant bushfires have occurred around the world, including near wine regions in Australia, Canada, South Africa and the USA, as a consequence of the warmer, drier conditions associated with climate change. Considerable research has subsequently been undertaken to determine the chemical, sensory and physiological consequences of grapevine exposure to smoke. The sensory attributes associated with smoke-tainted wine have been linked to the presence of several smoke-derived volatile phenols, such as guaiacols, syringols and cresols [2].

HEAT BERRY : Sensitivity of berries ripening to higher temperature and impact on phenolic compounds in wine

The grapevine is an important economical crop that is very sensitive to climate changes and microclimate. The observations made during the last decades at a vineyard scale all concur to show the impact of climate change on vine physiology, resulting in accelerated phenology and earlier harvest (Jones and Davis 2000). It is well-known that berry content is affected by the ambient temperature. While the first experiences were primarily conducted on the impact of temperature on anthocyanin accumulation in the grape, few studies have focused on others component of phenolic metabolism, such as tannins.