Macrowine 2021
IVES 9 IVES Conference Series 9 Oligosaccharides in red wines: could their structure and composition be influenced by the grape-growing

Oligosaccharides in red wines: could their structure and composition be influenced by the grape-growing

Abstract

Oligosaccharides have only recently been characterized in wine, and the information on composition and content is still limited. In wine, these molecules are mainly natural byproducts of the degradation of grape berry cell wall polysaccharides. Wine oligosaccharides present several physicochemical properties, being one relevant factor linked to the astringency perception of wines (1,2). A terroir can be defined as a grouping of homogeneous environmental units based on the typicality of the products obtained. This notion is particularly associated with wine, being the climate and the soil two of the major elements of terroir concept. Monastrell red wines, predominant varietal wines from the Southern of Spain, were elaborated with grapes from four different terroirs: Cañada Judío, Albatana, Bullas and Montealegre. Climate and soil data from different terroirs were gathered to properly distinguish them. Oligosaccharide fractions from wines were isolated, after removal of phenolic compounds, by high resolution size-exclusion chromatography. The glycosyl–linkages composition was determined by GC–MS of the partially methylated alditol acetates. Results show differences in the glycosyl–linkages composition of oligosaccharides from wines, according to their terroir. The molar percentage of glucose, rhamnose, arabinose, xylose and mannose residues exhibit marked differences depending on the terroir. The ratio of the terminal to the branched residues for Cañada Judío, Albatana, Bullas and Montealegre oligosaccharides is, respectively, 0.75, 0.85, 0.99 and 0.89. Proportions of oligosaccharides families have been calculated from glycosyl-linkage data (3,4,5). Montealegre wine clearly presents the lowest relative molar percentage for the oligosaccharides from yeasts (the sum of OligoGlucans and OligoMannans) and also for OligoXyloGlucans, whereas Bullas wine exhibits by far the lowest release of OligoRhamnogalacturonans. OligoArabinans and OligoArabiGalactans type II also show differences according to the terroir. All these data were treated by PCA to permit a best understanding. The projections on the first axis show obvious separation of Montealegre, whereas a clear separation of Albatana is observed in the projections on the second axis. The first and second principal components represent, respectively, 69% and 19% of the variability for samples. In summary, our results suggest the impact of “terroir” on the structure and the composition of wine oligosaccharide fraction, which could affect their physicochemical and sensory properties.

1.Quijada-Morín et al. (2014). Food Chem. 154, 44–51. 2.Boulet et al. (2016). Food Chem. 190, 357–363 3.Ducasse et al. (2011). J Agric Food Chem. 59, 6558–6567. 4.Ballou (1982). In Strathern, Jones & Broach (Eds.), Metabolism and gene expression (335–360), NY. 5.Fry et al. (1983). Plant Physiol. 89, 1–3.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Rafael Apolinar-Valiente*, Encarna Gómez-Plaza, José María Ros-García, Pascale Williams, Thierry Doco

*INRA Montpellier

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

New biological tools to control and secure malolactic fermentation in high pH wines

Originally, the role of the malolactic fermentation (MLF) was simply to improve the microbial stability of wine via biological deacidification. However, there is an accumulation of evidence to support the fact that lactic acid bacteria (LAB) also contribute positively to the taste and aroma of wine. Many different LAB enter into grape juice and wine from the surface of grape berries, cluster stems, vine leaves, soil and winery equipment. Due to the highly selective environment of juices and wine, only a few types of LAB are able to grow.

Light-struck taste in white wine: enological approach for its prevention

Light-struck taste is a defect prevalent in white wines bottled in clear glass light-exposed for a considerable amount of time leading to a loss of color and appearance of sulfur-like odors. The reaction involves riboflavin (RF), a highly photosensitive compound that undergoes to intermolecular photoreduction by the uptake of two electron equivalents from an external donor, the methionine. The reaction includes different steps forming methional which is extremely unstable and decomposes to methane thiol and acrolein. The reaction of two molecules of methane thiol yields dimethyl disulfide. Methane thiol is highly volatile, has a low perception threshold (2 to 10 µg/L in wine) and confers aroma-like rotten eggs or cabbage.

Prevention of wine oxidation during barrel aging: an innovative method to measure antioxidant

Wine oxidation is a problem that affects the freshness, the aromatic profile, the colour and also the mouthfeel of the wine. It mainly concerns white wines. Oxygen interactions with wine compounds lead to the phenomena cited above that are responsible for the depreciation of these wines. Barrel aging is a crucial step in the wine process because it allows many modifications as wine enrichment, colour stabilization, clarification and also a slow oxygenation of the wine. Effects of the oak barrel have to be known to prevent oxidation of the wine. We have been interested in the main antioxidant compounds released by oak barrels to the wine and we have developed an innovative method to reach directly these antioxidant compounds at the oak stave surface.

Study of the content of amino acids and biogenic amines in sparkling red wines

The production of red sparkling wines is lower in Spain in comparison with the winemaking of white or rosé sparkling wines. In red sparkling wine processing it is essential to obtain suitable base wines that should have moderate alcohol content, high acidity, good color values, an adequate mouth-feel and a sweet tannin. Grapes for sparkling wine production have to be harvested at low maturity stages, with lower alcohol contents and higher acidities, which will that the phenolic maturity of the grapes is also low, showing green tannins. This paper analyses different treatments in order to minimize these inconveniences: cold maceration-prefermentation and delestage to elaborate the grapes with lower maturity, must nanofiltration, and the partial osmosis of the wines made from grapes with an adequate maturity degree.

Elicitors used as a tool to increase stilbenes in grapes and wines

The economic importance of grapevine as a crop plant makes Vitis vinífera a good model system to study the improvement of the nutraceutical properties of food products (Vezulli et al. 2007). Stilbenes in general, and trans-resveratrol in particular, have been reported to be responsible for various beneficial effects. Resveratrol´s biological properties include antibacteria and antifungal effects, as well as cardioprotective, neuroprotective and anticâncer actions (Guerrero et al. 2010 ). Stilbenes can be induced by biotic and abiotic elicitors since they are phytoalexins (Bavaresco et al. 2001).