Macrowine 2021
IVES 9 IVES Conference Series 9 The role of tomato juice serum in malolactic fermentation in wine

The role of tomato juice serum in malolactic fermentation in wine

Abstract

Malolactic fermentation (MLF) is a common process in winemaking to reduce wine acidity, maintain microbial stability and modify wine aroma. However, successful MLF is often hampered by their sluggish or stuck activity of malolactic bacteria (MLB) which may be caused by nutrient deficiency, especially when MLB are inoculated after alcoholic fermentation (Alexandre et al., 2004; Lerm et al., 2010). Identification and characterization of essential nutrients and growth factors for MLB allows for production of highly efficient nutrient supplements for MLF. While the growth-inducing effect of tomato juice, the so-called ‘tomato juice factor’ (glycosylated pantothenic acid) on lactic acid bacteria in the culture medium without ethanol has been described a long time ago (Imamoto et al. 1972; Eto and Nakagawa 1975; Okada et al, 2000), still, the effect in high alcohol wine matrix remains to be elucidated. Aim: The aim of the current work was to study the possible role of TJF in inducing the malolactic conversion in wine. Materials and Methods: The synthetic grape must was fermented with EC-1118 wine yeast (Lalvin®, Lallemand Inc.) to the final ethanol concentration of 10-11%, pH 3.5 and glucose/fructose concentration of less than 3 g/L. The model wine was transferred into 100 ml fermenters and the whole freeze-dried tomato juice serum (TJS) or its molecular fractions obtained with size exclusion chromatography (SEC) were added. The wines were then inoculated with a commercial Oenococcus oeni strain VP41 (Lalvin®, Lallemand Inc.). The MLF was followed for 22 days and the conversion of malic acid to lactic acid was measured with HPLC. Glycosylated pantothenic acid was determined with indirect enzymatic method after hydrolysis of β-Glucosidase and liberated pantothenic acid was quantified by LC-MS. Results and Discussion: Our experiments showed that the addition of lyophilized TJS to model wine enabled to complete malolactic conversion in 18 days, while in control fermentation only 10 % of malic acid was consumed in the same time. The TJS was then fractionated using SEC and the effect of the collected fractions on MLF performance was tested using the same experimental setup. We observed the significant variation of MLF activity between different SEC fractions. The treatment of TJS with β-glucosidase revealed that from all pantothenic acid ~58% is glycosylated. These results suggest that TJS is a vital supplement, containing essential nutrients like glycosylated pantothenic acid for MLB, which results in quicker and more reliable MLF in wine.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Mary-Liis Kütt*, Ildar Nisamedtinov, Kaspar Kevvai, Triinu Kapp

*Competence Center of Food and Fermentation Technology

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Comprehensive exploration of wine aroma-related compounds as promoted by alternative vinification procedures in case of Zelen (Vitis vinifera L.) grapes processing

Not only vintner’s decisions in the vineyard, but also winemaker’s choices of technology approaches in the cellar play a significant role in the final wine style and quality. Whereas traditional technologies within chosen terroir are quite well explored and thus somehow predictable, there is no proper knowledge available on possible outcomes in case of implementing novel, alternative winemaking strategies. To reveal their effects on wine aroma compounds and sensory characteristics, two alternative strategies
(cryoextraction or addition of whole grape berries during last stages of fermentation) were compared to classical Vipava valley winemaking approach as normally used for an autochthonous variety Zelen. After separate vinification and bottling, all the experimental wines were subjected to semiquantitative metabolic profiling of volatile compounds (VOCs) by means of GC/MS and were then also sensorialy evaluated by pre-trained panel.

Effect of the winemaking technology on the phenolic compounds, foam parameters in sparklig wines

Contribution Sparkling wines elaborated following the traditional method undergo a second fermentation in closed bottles of base wines, followed by aging of wines with lees for at least 9 months. Most of the sparkling wines elaborated are white and rosé ones, although the production of red ones is highly increasing. One of the initial problems in red sparkling wine processing is to obtain suitable base wines that should have moderate alcohol content and astringency and adequate color intensity; which is difficult to obtain when grapes must be harvested at low phenolic and industrial maturity stage. The low phenolic maturity degree in the red grapes makes essential to choose an adequate winemaking methodology to obtain the base wines because the extracted polyphenols will vary according the winemaking technique: carbonic maceration or destemmed-crushed grapes.

On the losses of dissolved CO2 from laser-etched champagne glasses under standard tasting conditions

Under standard champagne tasting conditions, the complex interplay between the level of dissolved CO2 found in champagne, its temperature, the glass shape, and the bubbling rate, definitely impacts champagne tasting by modifying the neuro-physico-chemical mechanisms responsible for aroma release and flavor perception. Based on theoretical principles combining heterogeneous bubble nucleation, ascending bubble dynamics and mass transfer equations, a global model is proposed (depending on various parameters of both the wine and the glass itself), which quantitatively provides the progressive losses of dissolved CO2 from laser-etched champagne glasses.

Anthropogenic factors in modulations of fungal populations from grapes to wines and their repercussions on wine characteristics

The effects of anthropogenic activities on vineyard (different plant protections) and in winery
(pressing/clarification step, addition of sulfur dioxide) on fungal populations from grape to wine were studied. The studied anthropogenic activities modify the fungal diversity. Thus, lower biodiversity of grapes from organic modality was measured for the three vintages considered compared to biodiversity from ecophyto modality and conventional modality. The pressing / clarification steps strongly modify fungal populations and the influence of the winery flora is highlighted.

Effect of malolactic fermentation in barrels or stainless steel tanks on wine composition. Influence of the barrel toasting

Ellagitannin, anthocyanin and woody volatile composition of Cabernet Sauvignon wines aged in oak barrels for 12 months was evaluated. Depending on the container where malolactic fermentation (MLF) was carried out, two wine modalities were investigated: wines with MLF carried out in stainless steel tanks and barrel-fermented wines. Three toasting methods (medium toast, MT; medium toast with watering, MTAA; noisette) were considered for ageing of each wine modality. Sensory analyses (triangle and rating tests) were also performed. Two-way ANOVA of the raw experimental data revealed that the toasting method and the container where MLF took place, as well as the interaction between both factors, have a significant influence (p < 0.05) on ellagitannin, anthocyanin and woody volatile profiles of Cabernet Sauvignon wines.