Macrowine 2021
IVES 9 IVES Conference Series 9 The role of tomato juice serum in malolactic fermentation in wine

The role of tomato juice serum in malolactic fermentation in wine

Abstract

Malolactic fermentation (MLF) is a common process in winemaking to reduce wine acidity, maintain microbial stability and modify wine aroma. However, successful MLF is often hampered by their sluggish or stuck activity of malolactic bacteria (MLB) which may be caused by nutrient deficiency, especially when MLB are inoculated after alcoholic fermentation (Alexandre et al., 2004; Lerm et al., 2010). Identification and characterization of essential nutrients and growth factors for MLB allows for production of highly efficient nutrient supplements for MLF. While the growth-inducing effect of tomato juice, the so-called ‘tomato juice factor’ (glycosylated pantothenic acid) on lactic acid bacteria in the culture medium without ethanol has been described a long time ago (Imamoto et al. 1972; Eto and Nakagawa 1975; Okada et al, 2000), still, the effect in high alcohol wine matrix remains to be elucidated. Aim: The aim of the current work was to study the possible role of TJF in inducing the malolactic conversion in wine. Materials and Methods: The synthetic grape must was fermented with EC-1118 wine yeast (Lalvin®, Lallemand Inc.) to the final ethanol concentration of 10-11%, pH 3.5 and glucose/fructose concentration of less than 3 g/L. The model wine was transferred into 100 ml fermenters and the whole freeze-dried tomato juice serum (TJS) or its molecular fractions obtained with size exclusion chromatography (SEC) were added. The wines were then inoculated with a commercial Oenococcus oeni strain VP41 (Lalvin®, Lallemand Inc.). The MLF was followed for 22 days and the conversion of malic acid to lactic acid was measured with HPLC. Glycosylated pantothenic acid was determined with indirect enzymatic method after hydrolysis of β-Glucosidase and liberated pantothenic acid was quantified by LC-MS. Results and Discussion: Our experiments showed that the addition of lyophilized TJS to model wine enabled to complete malolactic conversion in 18 days, while in control fermentation only 10 % of malic acid was consumed in the same time. The TJS was then fractionated using SEC and the effect of the collected fractions on MLF performance was tested using the same experimental setup. We observed the significant variation of MLF activity between different SEC fractions. The treatment of TJS with β-glucosidase revealed that from all pantothenic acid ~58% is glycosylated. These results suggest that TJS is a vital supplement, containing essential nutrients like glycosylated pantothenic acid for MLB, which results in quicker and more reliable MLF in wine.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Mary-Liis Kütt*, Ildar Nisamedtinov, Kaspar Kevvai, Triinu Kapp

*Competence Center of Food and Fermentation Technology

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Merging fast sensory profiling with non-targeted GC-MS analysis for multifactorial experimental wine making

Wine aroma is influenced by several viticultural and oenological factors. In this study we used experimental wine making in a full factorial design to determine the impact of grapevine age, must turbidity, and yeast strain on the aroma of Vitis vinifera L. cv. Riesling wines. A recently developed, non-targeted SPME-GC-MS fingerprinting approach for wine volatiles was used. This approach includes the segmentation and mathematical transformation of chromatograms in combination with Parallel Factor Analysis (PARAFAC) and subsequent deconvolution of important chromatogram segments.

Effects of bottle closure type on sensory characteristics of Chasselas wines

Several winemaking operations, such as filtration, pumping, and racking, are known to potentially facilitate the incorporation of atmospheric O2 into the wine. Control of grape must oxidation is one key aspect in the management of white wine aroma expression, color stability and shelf-life extension. On the one hand, controlled must oxidation may help to remove highly reactive phenolic compounds, which otherwise could contribute to premature oxidation. And on the other hand, in certain cases of extreme protection of the must from O2 (e.g. pressing under inert atmosphere), it can help to preserve varietal aromas and natural must antioxidants.

Trans-resveratrol concentrations in wines Cabernet Sauvignon from Chile

This study evaluated the levels of trans-resveratrol in commercial wines made from Cabernet Sauvignon grapes from different valleys of Chile stilbenes. The Cabernet Sauvignon is the most planted variety in Chile, being 38% of the total vineyard country. Chile is the fourth largest wine exporter in the world, so it is important to evaluate the Cabernet-Sauvignon wines in their concentration levels of trans-resveratrol and its relation to the benefits provided to human health in moderate consumption. Evaluation comprises commercial wines from different valleys of Chile and its relationship with climatic characteristics, soil and vineyard handling.

Estimation of chemical age of red wines with the use of Fourier transform infrared spectroscopy (FT-IR) and chemometrics

The color of a red wine is one of the most important parameters of its quality, giving much information on its status, such as the grape variety used or the winemaking style. As the result of a complex equilibrium between different forms of anthocyanins and polymerization reactions which occur over the course of time, color can also serve as an indication of a wines’ age. For this purpose the “chemical age” i and ii indexes have been introduced by Somers in 1977. The chemical age index i measures the color absorbance after the addition of acetaldehyde while chemical index ii provides an indication of how much of the total red pigments are resistant to SO2 bleaching.

DNA and type of grain: which factor does better explain sensory differences of sessile and pedunculate oaks?

Sessile oak and pedunculate oak have shown several differences of interest for enological purposes. Tannic and aromatic composition among sessile oak or pedonculate oak has been well studied. Sessile oak is generally more aromatic than pedunculated, while the later is more tannic. This scientific point of view is rarely applied to classify oak in cooperages. Most coopers use the type of grain to distinguish wide and thin grain.