Macrowine 2021
IVES 9 IVES Conference Series 9 The role of tomato juice serum in malolactic fermentation in wine

The role of tomato juice serum in malolactic fermentation in wine

Abstract

Malolactic fermentation (MLF) is a common process in winemaking to reduce wine acidity, maintain microbial stability and modify wine aroma. However, successful MLF is often hampered by their sluggish or stuck activity of malolactic bacteria (MLB) which may be caused by nutrient deficiency, especially when MLB are inoculated after alcoholic fermentation (Alexandre et al., 2004; Lerm et al., 2010). Identification and characterization of essential nutrients and growth factors for MLB allows for production of highly efficient nutrient supplements for MLF. While the growth-inducing effect of tomato juice, the so-called ‘tomato juice factor’ (glycosylated pantothenic acid) on lactic acid bacteria in the culture medium without ethanol has been described a long time ago (Imamoto et al. 1972; Eto and Nakagawa 1975; Okada et al, 2000), still, the effect in high alcohol wine matrix remains to be elucidated. Aim: The aim of the current work was to study the possible role of TJF in inducing the malolactic conversion in wine. Materials and Methods: The synthetic grape must was fermented with EC-1118 wine yeast (Lalvin®, Lallemand Inc.) to the final ethanol concentration of 10-11%, pH 3.5 and glucose/fructose concentration of less than 3 g/L. The model wine was transferred into 100 ml fermenters and the whole freeze-dried tomato juice serum (TJS) or its molecular fractions obtained with size exclusion chromatography (SEC) were added. The wines were then inoculated with a commercial Oenococcus oeni strain VP41 (Lalvin®, Lallemand Inc.). The MLF was followed for 22 days and the conversion of malic acid to lactic acid was measured with HPLC. Glycosylated pantothenic acid was determined with indirect enzymatic method after hydrolysis of β-Glucosidase and liberated pantothenic acid was quantified by LC-MS. Results and Discussion: Our experiments showed that the addition of lyophilized TJS to model wine enabled to complete malolactic conversion in 18 days, while in control fermentation only 10 % of malic acid was consumed in the same time. The TJS was then fractionated using SEC and the effect of the collected fractions on MLF performance was tested using the same experimental setup. We observed the significant variation of MLF activity between different SEC fractions. The treatment of TJS with β-glucosidase revealed that from all pantothenic acid ~58% is glycosylated. These results suggest that TJS is a vital supplement, containing essential nutrients like glycosylated pantothenic acid for MLB, which results in quicker and more reliable MLF in wine.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Mary-Liis Kütt*, Ildar Nisamedtinov, Kaspar Kevvai, Triinu Kapp

*Competence Center of Food and Fermentation Technology

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Use of computational modelling for selecting adsorbents for improved fining of wine

The occurrence of faults and taints in wine, such as those caused by microbial spoilage or various taints, have resulted in significant financial losses to wine producers. The wine industry commits significant financial resources towards fining and taint removal processes each year. Fining involves the addition of one or more adsorptive substrates to juice or wine to bind certain components, thus reducing their concentration [1]. However, these processes are often not selective and can also remove desirable flavour and aroma compounds.

Merging fast sensory profiling with non-targeted GC-MS analysis for multifactorial experimental wine making

Wine aroma is influenced by several viticultural and oenological factors. In this study we used experimental wine making in a full factorial design to determine the impact of grapevine age, must turbidity, and yeast strain on the aroma of Vitis vinifera L. cv. Riesling wines. A recently developed, non-targeted SPME-GC-MS fingerprinting approach for wine volatiles was used. This approach includes the segmentation and mathematical transformation of chromatograms in combination with Parallel Factor Analysis (PARAFAC) and subsequent deconvolution of important chromatogram segments.

Characterizing the effects of nitrogen on grapevines with different scion/rootstock combinations: agronomic, metabolomic and transcriptomic approaches

Most vineyards are grafted and include a variety (Vitis vinifera) grafted over a wild Vitis rootstock (hybrids of V. berlandieri, riparia and rupestris). Grape berry quality at harvest depends on a subtle balance between acidity and the concentrations of sugars, polyphenols and precursors of aroma compounds. The mechanisms controlling the balance of sugars/acids/polyphenols are influenced by the abiotic environment, in particular nitrogen supply, and interact with the genotypes of both the scion variety and the rootstock. Previous work suggests that some of the effects of water stress are in fact linked to a nitrogen deficiency driven indirectly by the reduction of water absorption.

Study of the content of amino acids and biogenic amines in sparkling red wines

The production of red sparkling wines is lower in Spain in comparison with the winemaking of white or rosé sparkling wines. In red sparkling wine processing it is essential to obtain suitable base wines that should have moderate alcohol content, high acidity, good color values, an adequate mouth-feel and a sweet tannin. Grapes for sparkling wine production have to be harvested at low maturity stages, with lower alcohol contents and higher acidities, which will that the phenolic maturity of the grapes is also low, showing green tannins. This paper analyses different treatments in order to minimize these inconveniences: cold maceration-prefermentation and delestage to elaborate the grapes with lower maturity, must nanofiltration, and the partial osmosis of the wines made from grapes with an adequate maturity degree.

Modulating role of SO2 in white wine protein haze formation

Despite the extensive research performed during the last decades, the multifactorial mechanism responsible for the white wine protein haze formation is not fully characterized. Herein, a new model is proposed, which is based on the experimental identification of sulfur dioxide as a major modulating factor inducing wine protein haze upon heating. As opposed to other reducing agents, such as 2-mercaptoethanol, dithiothreitol and tris(2-carboxyethyl)phosphine hydrochloride (TCEP), the addition of SO2 to must/wine upon heating cleaves intraprotein disulfide bonds, hinders thiol-disulfide exchange during protein interactions and can lead to the formation of novel inter/intraprotein disulfide bonds. Those are eventually responsible for wine protein aggregation which follows a nucleation-growth kinetic model as shown by dynamic light scattering [1].