Macrowine 2021
IVES 9 IVES Conference Series 9 Glutathione content evolution during spontaneous alcoholic fermentations of Sangiovese grapes

Glutathione content evolution during spontaneous alcoholic fermentations of Sangiovese grapes

Abstract

Glutathione is a tripeptide (γ-Glu-Cys-Gly), which can occur in grapes, in must and in wine prevalently in the reduced form as well as in the oxidized form as glutathione disulfide. The importance of the reduced form of glutathione lies in its antioxidant activity. In must, it limits browning by reducing o-quinones produced by polyphenol oxidase activity on hydroxycinnamic acids; in wine, it exerts a protective effect on various aromatic compounds. Glutathione concentration in wine is lower than in grape juice and variable as it depends on several factors, ranging from the native content of grapes to winemaking technique. During alcoholic fermentation, Saccharomyces cerevisiae metabolism modifies to different extent the native concentration of glutathione in must, depending on the strain. In this study, the evolution of the glutathione content during three spontaneous alcoholic fermentations of Sangiovese grapes (vintage 2015), carried out at commercial-scale in a winery located in Val d’Orcia (Tuscany), was monitored. From a microbiological point of view, S. cerevisiae dominated all the fermentation phases, since non-Saccharomyces yeast did not reach significant population densities. However, a great intraspecific genetic diversity was found within the S. cerevisiae populations. In freshly pressed musts, glutathione concentration ranged from 4 to 7mg/L, and consisted predominantly of the oxidized form. During alcoholic fermentation, glutathione concentration resulted highly variable: in the first days, in correspondence of yeast exponential growth phase, it decreased, possibly due to the consumption of glutathione by yeast cells in the active growth phase; successively, glutathione content increased until the end of alcoholic fermentation, suggesting a glutathione release from yeast cells in correspondence to cell autolysis into the wine; at the end of alcoholic fermentation, glutathione contents of wines ranged from 19 to 23mg/L, and the reduced form represented about 60% of the total. The results suggest that the final glutathione concentration in wine is mostly dependent on the amount of glutathione released by yeast cells rather than on that derived from grapes at the vintage.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Silvia Mangani*, Eleonora Mari, Giacomo Buscioni, Martina Cerretelli, Massimo Vincenzini, Simona Guerrini, Yuri Romboli

*Food Micro Team

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Impact of elemental sulfur (S0) residues in Sauvignon blanc juice on the formation of the varietal thiols 3-mercapto hexanol and 3-mercaptohexyl acetate

Elemental sulfur is a fungicide used by grape growers to control the development of powdery mildew, caused by the fungus Erysiphe necator. This compound is effective, cheap and has a low toxicity with no withholding period recommended. However, high levels of S0 residues in the harvested grapes can lead to the formation of reductive sulfur compounds that can impart taints and faults to the wine. Hydrogen sulphide (H2S) is a very volatile and unpleasant sulfur compound which formation is connected to high residues of S0 in juice (10 – 100 mg/L).

On the losses of dissolved CO2 from laser-etched champagne glasses under standard tasting conditions

Under standard champagne tasting conditions, the complex interplay between the level of dissolved CO2 found in champagne, its temperature, the glass shape, and the bubbling rate, definitely impacts champagne tasting by modifying the neuro-physico-chemical mechanisms responsible for aroma release and flavor perception. Based on theoretical principles combining heterogeneous bubble nucleation, ascending bubble dynamics and mass transfer equations, a global model is proposed (depending on various parameters of both the wine and the glass itself), which quantitatively provides the progressive losses of dissolved CO2 from laser-etched champagne glasses.

South Africa’s top 10 Sauvignon blanc wines. How do the chemical and sensory profiles compare?

FNB Top 10 Sauvignon Blanc competition, presented by the Sauvignon Blanc Interest Group of South Africa and sponsored by First National Bank, is the country’s foremost platform for producers of this cultivar to showcase and benchmark their wines. Wines entered in the competition originated from all over the winegrowing regions of the country and the winning wines showed good representation of quality South African Sauvignon blanc wines. The ten selected wines were subjected to various chemical analyses including volatile thiol and methoxypyrazine determination, while the sensory profile of each wine was determined using projective mapping.

The impact of branched chain and aromatic amino acids on fermentation kinetics and aroma biosynthesis by wine yeast Saccharomyces cerevisiae

One of the major determinants of wine quality is the aroma. Wine aroma is the human perception of the matrix of grape and yeast derived volatiles and their interaction that contribute to flavour wine. Most common are higher alcohols, ester and aldehydes. In previous studies the formation of characteristic volatile compounds have been linked to the metabolism of branched-chain and aromatic amino acids
(BCAAs) in synthetic grape must. Here we report on an investigation to assess the impact of the initial amino acid concentration on the production of aroma compounds by the industrial yeast VIN13 grown in both synthetic and real grape musts.

Effect of different foliar nitrogen applications on the must amino acids and glutathione composition in Cabernet Sauvignon vineyard

Cabernet Sauvignon is one of the most important winegrape varieties in Chile. However, temperature raise and decreased rainfall due to climate change can lead to grape quality decrease in certain areas. Amino acids are essential as nitrogen source for yeast but also directly affect grape quality serving as precursors of certain volatile compounds that enhance the wine bouquet. Besides, glutathione is an important tripeptide acting as antioxidant, preventing the appearance of browning pigments in must and exerts a protective effect in volatile compounds.