Macrowine 2021
IVES 9 IVES Conference Series 9 Glutathione content evolution during spontaneous alcoholic fermentations of Sangiovese grapes

Glutathione content evolution during spontaneous alcoholic fermentations of Sangiovese grapes

Abstract

Glutathione is a tripeptide (γ-Glu-Cys-Gly), which can occur in grapes, in must and in wine prevalently in the reduced form as well as in the oxidized form as glutathione disulfide. The importance of the reduced form of glutathione lies in its antioxidant activity. In must, it limits browning by reducing o-quinones produced by polyphenol oxidase activity on hydroxycinnamic acids; in wine, it exerts a protective effect on various aromatic compounds. Glutathione concentration in wine is lower than in grape juice and variable as it depends on several factors, ranging from the native content of grapes to winemaking technique. During alcoholic fermentation, Saccharomyces cerevisiae metabolism modifies to different extent the native concentration of glutathione in must, depending on the strain. In this study, the evolution of the glutathione content during three spontaneous alcoholic fermentations of Sangiovese grapes (vintage 2015), carried out at commercial-scale in a winery located in Val d’Orcia (Tuscany), was monitored. From a microbiological point of view, S. cerevisiae dominated all the fermentation phases, since non-Saccharomyces yeast did not reach significant population densities. However, a great intraspecific genetic diversity was found within the S. cerevisiae populations. In freshly pressed musts, glutathione concentration ranged from 4 to 7mg/L, and consisted predominantly of the oxidized form. During alcoholic fermentation, glutathione concentration resulted highly variable: in the first days, in correspondence of yeast exponential growth phase, it decreased, possibly due to the consumption of glutathione by yeast cells in the active growth phase; successively, glutathione content increased until the end of alcoholic fermentation, suggesting a glutathione release from yeast cells in correspondence to cell autolysis into the wine; at the end of alcoholic fermentation, glutathione contents of wines ranged from 19 to 23mg/L, and the reduced form represented about 60% of the total. The results suggest that the final glutathione concentration in wine is mostly dependent on the amount of glutathione released by yeast cells rather than on that derived from grapes at the vintage.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Silvia Mangani*, Eleonora Mari, Giacomo Buscioni, Martina Cerretelli, Massimo Vincenzini, Simona Guerrini, Yuri Romboli

*Food Micro Team

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Intelligent article to control the internal pressure in continue in bottles

An intelligent packaging might, among others, provide information and allow monitoring of the quality of the packed product or its surrounding environment. A recent project on micro-flow wine bottles closed with aluminium screw cap and tightness liner, highlighted the importance of monitoring the internal overpressure continuously, in real-time and at least for 72 hours, since the internal pressure on the tightness liner and the micro-flow are related. Real-time and continuous measurements are not the standard methods of measurement of the overpressure, yet. The most used equipment for the determination of the pressure in wine bottle is the aphrometer, a destructive device that supplies a single value of pressure.

Use of computational modelling for selecting adsorbents for improved fining of wine

The occurrence of faults and taints in wine, such as those caused by microbial spoilage or various taints, have resulted in significant financial losses to wine producers. The wine industry commits significant financial resources towards fining and taint removal processes each year. Fining involves the addition of one or more adsorptive substrates to juice or wine to bind certain components, thus reducing their concentration [1]. However, these processes are often not selective and can also remove desirable flavour and aroma compounds.

Influence of preflowering basal leaf removal on aromatic composition of cv. Tempranillo wine from semiarid climate (Extremadura Western Spain)

Abstract In this work the effects of early leaf removal performed manually at preflowering phenological stage, on the volatile composition of Tempranillo (Vitis vinifera L.) wines were studied. From 2009-2011 vintages 34 wine volatile compounds were identified and quantified by gas chromatography-mass spectrometry (GC-MS) where early leaf removal only modified 25 of them. The total C6 compounds, acetates and volatiles acids (with exception of isobutyric acid) were affected by defoliation, whereas alcohols and esters showed a minor effect. Furthermore the vintage effect also was shown.

Novel contribution to the study of mouth-feel properties in wines

In general, there is a well-established lexicon related to wine aroma and taste properties; however mouth-feel-related vocabulary usually includes heterogeneous, multimodal and personalized terms. Gawel et al.
(2000) published a wheel related to mouthfeel properties of red wine. However, its use in scientific publications has been limited. The authors accepted that the approach had certain limitations as it included redundant and terms with hedonic tone and some others were absent. It is of high interest to generate a mouth-feel lexicon and finding the chemical compound or group of compounds responsible for such properties in red wine. In the present work a chemical fractionation method has been developed.

Foam characteristics of white, rosé and red sparkling wines elaborated by the champenoise method

Contribution Foam is the characteristic that differentiates sparkling wines from still wines, being the first sensory attribute that tasters and consumers perceive and that determines the final quality of sparkling wines [1]. The foaming properties mainly depend on the chemical composition of wines [2-3], and different factors involved in wine composition will have an effect on foam quality. In Spain, the sparkling wine market focuses on the production of white and rosé sparkling wine, with very low production of red sparkling wines. However, this type of wines is elaborated in countries like Australia, South-Africa, Argentina, Italy or Portugal, with a great acceptance by consumers. No studies on the foaming characteristics of red sparkling wines have been found.