Macrowine 2021
IVES 9 IVES Conference Series 9 Glutathione content evolution during spontaneous alcoholic fermentations of Sangiovese grapes

Glutathione content evolution during spontaneous alcoholic fermentations of Sangiovese grapes

Abstract

Glutathione is a tripeptide (γ-Glu-Cys-Gly), which can occur in grapes, in must and in wine prevalently in the reduced form as well as in the oxidized form as glutathione disulfide. The importance of the reduced form of glutathione lies in its antioxidant activity. In must, it limits browning by reducing o-quinones produced by polyphenol oxidase activity on hydroxycinnamic acids; in wine, it exerts a protective effect on various aromatic compounds. Glutathione concentration in wine is lower than in grape juice and variable as it depends on several factors, ranging from the native content of grapes to winemaking technique. During alcoholic fermentation, Saccharomyces cerevisiae metabolism modifies to different extent the native concentration of glutathione in must, depending on the strain. In this study, the evolution of the glutathione content during three spontaneous alcoholic fermentations of Sangiovese grapes (vintage 2015), carried out at commercial-scale in a winery located in Val d’Orcia (Tuscany), was monitored. From a microbiological point of view, S. cerevisiae dominated all the fermentation phases, since non-Saccharomyces yeast did not reach significant population densities. However, a great intraspecific genetic diversity was found within the S. cerevisiae populations. In freshly pressed musts, glutathione concentration ranged from 4 to 7mg/L, and consisted predominantly of the oxidized form. During alcoholic fermentation, glutathione concentration resulted highly variable: in the first days, in correspondence of yeast exponential growth phase, it decreased, possibly due to the consumption of glutathione by yeast cells in the active growth phase; successively, glutathione content increased until the end of alcoholic fermentation, suggesting a glutathione release from yeast cells in correspondence to cell autolysis into the wine; at the end of alcoholic fermentation, glutathione contents of wines ranged from 19 to 23mg/L, and the reduced form represented about 60% of the total. The results suggest that the final glutathione concentration in wine is mostly dependent on the amount of glutathione released by yeast cells rather than on that derived from grapes at the vintage.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Silvia Mangani*, Eleonora Mari, Giacomo Buscioni, Martina Cerretelli, Massimo Vincenzini, Simona Guerrini, Yuri Romboli

*Food Micro Team

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Trans-resveratrol concentrations in wines Cabernet Sauvignon from Chile

This study evaluated the levels of trans-resveratrol in commercial wines made from Cabernet Sauvignon grapes from different valleys of Chile stilbenes. The Cabernet Sauvignon is the most planted variety in Chile, being 38% of the total vineyard country. Chile is the fourth largest wine exporter in the world, so it is important to evaluate the Cabernet-Sauvignon wines in their concentration levels of trans-resveratrol and its relation to the benefits provided to human health in moderate consumption. Evaluation comprises commercial wines from different valleys of Chile and its relationship with climatic characteristics, soil and vineyard handling.

Impact of varying ethanol and carbonation levels on the odor threshold of 1,1,6-trimethyl-1,2-dihydronaphtalene (petrol off-flavor) and role of berry size and Riesling clones

1,1,6-trimethyl-1,2-dihydronaphtelene (TDN) evokes the odor of “petrol” in wine, especially in the variety Riesling. Increasing UV-radiation due to climate change intensifies formation of carotenoids in the berry skins and an increase of TDN-precursors1. Exploring new viticultural and oenological strategies to limit TDN formation in the future requires precise knowledge of TDN thresholds in different matrices. Thresholds reported in the literature vary substantially between 2 µg/L up to 20 µg/L2,3,4 due to the use of different methods. As Riesling grapes are used for very different wine styles such as dry, sweet or sparkling wines, it is essential to study the impact of varying ethanol and carbonation levels.

Proteomic and activity characterization of exocellular laccases from three Botrytis cinerea strains

Botrytis cinerea is a fungus that causes common infection in grapes and other fruits. In winemaking, its presence can be both considered desirable in the case of noble rot infection or undesirable when grey rot is developed. This fungus produces an extracellular enzyme known as laccase which is able to cause oxidation of phenolic compounds present in must and wine, causing most of the times a decrease in its quality and problems during the winemaking process [1]. Material and methods: Three B. cinerea strains (B0510, VA612 and RM344) were selected and grown in a liquid medium adapted from one previously described [2]. The enzyme was isolated by tangential ultrafiltration of the culture medium using a QuixStand system equipped with a 30 KDa filtration membrane.

Bentonite fining in cold wines: prediction tests, reduced efficiency and possibilities to avoid additional fining treatments

Bentonite fining is widely used to prevent protein haze in white wines. Most wineries use laboratory-scale fining trials to define the appropriate amount of bentonite to be used in the cellar. Those pre-tests need to mimic as much as possible the industrial scale fining procedure to determine the exact amount of bentonite necessary for protein stability. Nevertheless it is frequent that, after fining with the recommended amount of bentonite, wines appear still unstable and need an additional fining treatment. It remains a major challenge to understand why the same wine, fined with the same dosage of the same bentonite, achieves stability in the lab, but not in the cellar.

Cover crops influence on soil N availability and grapevine N status, and its relationship with biogenic

The type of soil management, tillage versus cover crops, can modify the soil microbial activity, which causes the mineralization of organic N to NO3–N and, therefore, may change the soil NO3–N availability in vineyard. The soil NO3–N availability could influence the grapevine nutritional status and the grape amino acid composition. Amino acids are precursors of biogenic amines, compounds mainly formed during the malolactic fermentation. Biogenic amines have negative effects on consumer health and on the wine organoleptic quality. The objective was to study if the effect of conventional tillage and two different cover crops (leguminous versus gramineous) on grapevine N status, could relate to the wine biogenic amines composition.