Macrowine 2021
IVES 9 IVES Conference Series 9 Glutathione content evolution during spontaneous alcoholic fermentations of Sangiovese grapes

Glutathione content evolution during spontaneous alcoholic fermentations of Sangiovese grapes

Abstract

Glutathione is a tripeptide (γ-Glu-Cys-Gly), which can occur in grapes, in must and in wine prevalently in the reduced form as well as in the oxidized form as glutathione disulfide. The importance of the reduced form of glutathione lies in its antioxidant activity. In must, it limits browning by reducing o-quinones produced by polyphenol oxidase activity on hydroxycinnamic acids; in wine, it exerts a protective effect on various aromatic compounds. Glutathione concentration in wine is lower than in grape juice and variable as it depends on several factors, ranging from the native content of grapes to winemaking technique. During alcoholic fermentation, Saccharomyces cerevisiae metabolism modifies to different extent the native concentration of glutathione in must, depending on the strain. In this study, the evolution of the glutathione content during three spontaneous alcoholic fermentations of Sangiovese grapes (vintage 2015), carried out at commercial-scale in a winery located in Val d’Orcia (Tuscany), was monitored. From a microbiological point of view, S. cerevisiae dominated all the fermentation phases, since non-Saccharomyces yeast did not reach significant population densities. However, a great intraspecific genetic diversity was found within the S. cerevisiae populations. In freshly pressed musts, glutathione concentration ranged from 4 to 7mg/L, and consisted predominantly of the oxidized form. During alcoholic fermentation, glutathione concentration resulted highly variable: in the first days, in correspondence of yeast exponential growth phase, it decreased, possibly due to the consumption of glutathione by yeast cells in the active growth phase; successively, glutathione content increased until the end of alcoholic fermentation, suggesting a glutathione release from yeast cells in correspondence to cell autolysis into the wine; at the end of alcoholic fermentation, glutathione contents of wines ranged from 19 to 23mg/L, and the reduced form represented about 60% of the total. The results suggest that the final glutathione concentration in wine is mostly dependent on the amount of glutathione released by yeast cells rather than on that derived from grapes at the vintage.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Silvia Mangani*, Eleonora Mari, Giacomo Buscioni, Martina Cerretelli, Massimo Vincenzini, Simona Guerrini, Yuri Romboli

*Food Micro Team

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Study of the volatil profile of minority white varieties

The genetic material preservation is a priority issue in winemaking research. The recovery of minority grape varieties can control the genetic erosion, contributing also to preserve wine typical characteristics. In D.O.Ca. Rioja (Spain) the number of grown white varieties has been very limited, representing Viura the 91% of the cultivated white grape area in 2005, while the others, Garnacha Blanca and Malvasía riojana, hardly were grown. For this reason, a recovery and characterization study of plant material was carried out in this region. In 2008, the results obtained allowed the authorization of three minority white varieties: Tempranillo Blanco, Maturana Blanca and Turruntés.

Analysis of the oenological potentials of different oak forests in Hungary

Like France, Hungary has many oak forests used for making barrels since many years. But if the differences between the woods of the North, the East and the South-West forests of France are well known, this is probably not the case of Hungarian forests. However taking into account the essential differences of climates and soils, differences must be significant and the general name “Hungarian oak” must not have any real meaning. We have studied precisely (determination of concentrations of volatile and non-volatile wood compounds, anatomical criteria, measurement of antioxidant capacity) of oaks collected from northeastern Hungary and others collected from the Danube valley in the northwest of the country.

Using combinations of recombinant pectinases to elucidate the deconstruction of the polysaccharide‐rich grape cell wall during winemaking

The effectiveness of enzyme-mediated maceration processes in red winemaking relies on a clear picture of the target (berry cell wall structure) to achieve the optimum combination of specific enzymes to be used. However, we lack the information on both essential factors of the reaction (i.e. specific activities in commercial enzyme preparation and the cell wall structure of berry tissue). In this study, the different combinations of pure recombinant enzymes and the recently validated high throughput cell wall profiling tools were applied to extend our knowledge on the grape berry cell wall polymeric deconstruction during the winemaking following a combinatorial enzyme treatment design.

Comparative proteomic analysis of wines made from Botrytis cinerea infected and healthy grapes reveal interesting parallels to the gushing phenomenon in sparkling wine

In addition to aroma compounds also protein composition strongly influences the quality of wines. Proteins of wine derive mainly from the plant Vitis vinifera and may be influenced by abiotic stress as well as fermentation conditions or fining. Additionally, fungal infections can affect the protein content as well by introducing fungal proteins or affecting grape protein composition. An infection of the vine with the plant pathogenic fungus Botrytis (B.) cinerea was shown to cause a degradation of proteins in the resulting wine. Moreover, it influences the foaming properties in sparkling wine.

Phenolic profiles of minor red grape cultivars autochthonous from the Spanish region of La Mancha

The phenolic profiles of little known red grape cultivars, namely Garnacho, Moribel and Tinto Fragoso, which are autochthonous from the Spanish region of La Mancha (ca. 600,000 ha of vineyards) have been studied over the consecutive seasons of years 2013 and 2014. The study was separately performed over the skins, the pulp and the seeds, and comprised the following phenolic types: anthocyanins, flavonols, hydroxycinnamic acid derivatives (HCADs), total proanthocyanidins (PAs) and their structural features. The selected grape cultivars belong to the Vine Germplasm Bank created in this region in order to preserve the great diversity of genotypes grown in La Mancha.