Macrowine 2021
IVES 9 IVES Conference Series 9 Glutathione content evolution during spontaneous alcoholic fermentations of Sangiovese grapes

Glutathione content evolution during spontaneous alcoholic fermentations of Sangiovese grapes

Abstract

Glutathione is a tripeptide (γ-Glu-Cys-Gly), which can occur in grapes, in must and in wine prevalently in the reduced form as well as in the oxidized form as glutathione disulfide. The importance of the reduced form of glutathione lies in its antioxidant activity. In must, it limits browning by reducing o-quinones produced by polyphenol oxidase activity on hydroxycinnamic acids; in wine, it exerts a protective effect on various aromatic compounds. Glutathione concentration in wine is lower than in grape juice and variable as it depends on several factors, ranging from the native content of grapes to winemaking technique. During alcoholic fermentation, Saccharomyces cerevisiae metabolism modifies to different extent the native concentration of glutathione in must, depending on the strain. In this study, the evolution of the glutathione content during three spontaneous alcoholic fermentations of Sangiovese grapes (vintage 2015), carried out at commercial-scale in a winery located in Val d’Orcia (Tuscany), was monitored. From a microbiological point of view, S. cerevisiae dominated all the fermentation phases, since non-Saccharomyces yeast did not reach significant population densities. However, a great intraspecific genetic diversity was found within the S. cerevisiae populations. In freshly pressed musts, glutathione concentration ranged from 4 to 7mg/L, and consisted predominantly of the oxidized form. During alcoholic fermentation, glutathione concentration resulted highly variable: in the first days, in correspondence of yeast exponential growth phase, it decreased, possibly due to the consumption of glutathione by yeast cells in the active growth phase; successively, glutathione content increased until the end of alcoholic fermentation, suggesting a glutathione release from yeast cells in correspondence to cell autolysis into the wine; at the end of alcoholic fermentation, glutathione contents of wines ranged from 19 to 23mg/L, and the reduced form represented about 60% of the total. The results suggest that the final glutathione concentration in wine is mostly dependent on the amount of glutathione released by yeast cells rather than on that derived from grapes at the vintage.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Silvia Mangani*, Eleonora Mari, Giacomo Buscioni, Martina Cerretelli, Massimo Vincenzini, Simona Guerrini, Yuri Romboli

*Food Micro Team

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

New biological tools to control and secure malolactic fermentation in high pH wines

Originally, the role of the malolactic fermentation (MLF) was simply to improve the microbial stability of wine via biological deacidification. However, there is an accumulation of evidence to support the fact that lactic acid bacteria (LAB) also contribute positively to the taste and aroma of wine. Many different LAB enter into grape juice and wine from the surface of grape berries, cluster stems, vine leaves, soil and winery equipment. Due to the highly selective environment of juices and wine, only a few types of LAB are able to grow.

Correlations between sensory characteristics and colloidal content in dry white wines

Must clarification is an important step occurring just after grape extraction in the elaboration of white wine, consisting in a solid-liquid separation. Traditionally, low must turbidity, around 50-150 NTU, is generally reached in white winemaking in order to prevent reductive aromas and facilitating alcoholic fermentation. Alternatively, a higher turbidity (300 NTU or above) can be sought for reasons such as a better expression of grapes identity (terroir), or for getting a must matrix that could supposedly lead to wines having greater ageing potential.

Impact of drought stress on concentration and composition of wine proteins in Riesling

Protein haze in white wines is a major technological and economic problem of the wine industry. Field tests were carried out in steep slope vineyards planted with Riesling grapes over 3 dry growing seasons to study the effect of drought stress on the concentration of proteins in the resulting wines. Plots suffering from drought stress were compared with surrounding drip irrigated plots. Riesling grapes were processed into wines by conventional procedures. Protein amounts of the isolated wine colloids of the stressed samples were always higher than those of the watered samples(mean watered 13.8 ± 0.44, mean stressed 17.4 ± 0.40 g 100 g-1). As a consequence, higher bentonite doses were needed to achieve protein haze stability of the drought stressed treatments.

Trans-resveratrol concentrations in wines Cabernet Sauvignon from Chile

This study evaluated the levels of trans-resveratrol in commercial wines made from Cabernet Sauvignon grapes from different valleys of Chile stilbenes. The Cabernet Sauvignon is the most planted variety in Chile, being 38% of the total vineyard country. Chile is the fourth largest wine exporter in the world, so it is important to evaluate the Cabernet-Sauvignon wines in their concentration levels of trans-resveratrol and its relation to the benefits provided to human health in moderate consumption. Evaluation comprises commercial wines from different valleys of Chile and its relationship with climatic characteristics, soil and vineyard handling.

Impact of varying ethanol and carbonation levels on the odor threshold of 1,1,6-trimethyl-1,2-dihydronaphtalene (petrol off-flavor) and role of berry size and Riesling clones

1,1,6-trimethyl-1,2-dihydronaphtelene (TDN) evokes the odor of “petrol” in wine, especially in the variety Riesling. Increasing UV-radiation due to climate change intensifies formation of carotenoids in the berry skins and an increase of TDN-precursors1. Exploring new viticultural and oenological strategies to limit TDN formation in the future requires precise knowledge of TDN thresholds in different matrices. Thresholds reported in the literature vary substantially between 2 µg/L up to 20 µg/L2,3,4 due to the use of different methods. As Riesling grapes are used for very different wine styles such as dry, sweet or sparkling wines, it is essential to study the impact of varying ethanol and carbonation levels.