Macrowine 2021
IVES 9 IVES Conference Series 9 Influence of methyl jasmonate foliar application to vineyard on grape volatile composition over three consecutive vintages

Influence of methyl jasmonate foliar application to vineyard on grape volatile composition over three consecutive vintages

Abstract

An alternative to improve grape quality is the application to the vineyard of elicitors. Although these compounds were first used to increase resistance of plants against pathogens, it has been found that they are also able to induce mechanisms involved in the synthesis of phenolic compounds and some amino acids. However, researches about the influence of elicitors on grape volatile composition are scarcely. Therefore, the aim of this work was to study the influence of methyl jasmonate (MeJ) foliar application on grape aroma composition over three consecutive vintages. MeJ was applied to Tempranillo grapevines at a concentration of 10 mM in 2013, 2014, and 2015 years. Control plants were sprayed with water. The treatments were applied to grapevine twice, at veraison and one week later, and for each application, 200 mL/plant were sprayed over leaves. The treatments were carried out in triplicate and were arranged in a complete randomized block design. Grape volatile composition (terpenes, C13 norisoprenoids, esters, benzenoids, and C6 compounds) was determined by HS-SPME-GC-MS. The statistical analysis was performed by ANOVA, considering grape volatile compounds as dependent variables and treatment and vintage as categorical factors. The results showed that the grape volatile content was different for each year. Regarding treatment factor, in the first year of study, foliar application of MeJ positively affected the presence of p-cymene, methyl jasmonate, and hexanal, and negatively to the content of 2-hexen-1-ol acetate, (z)-3-hexen-1-ol, and n-hexanol; the rest of the compounds were unaffected by the treatment. However, in the second year, the application of MeJ to grapevine showed a greater influence on the presence of volatile compounds in grape. The formation of all terpenes, with the exception of p-cymene, was negatively affected by the MeJ foliar application. For C13 norisoprenoids, the effect of the treatment was also negative for
norisoprenoids was unaffected by the MeJ treatment. Likewise, the grape level of esters and benzenoids was also negatively affected by the MeJ treatment. Finally, the MeJ treatment increased the presence of n-hexanol while (z)-3-hexen-1-ol was reduced; for the remaining C6 compounds no effect of the MeJ treatment was observed. It is noteworthy that the influence of the MeJ foliar application was positive for the grape volatile composition in the third year of study. The foliar application of MeJ favoured the synthesis of ten volatile compounds that are considered positive for grape aroma, while the rest of the positive compounds were unaffected, with the exception of geranyl acetone. In conclusion, grape volatile content and the effect of MeJ foliar application on it were dependent on the vintage.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Teresa Garde-Cerdan*, Elisa Baroja, Javier Portu, Pilar Santamaría, Rosa López

*Instituto de Ciencias de la Vid y del Vino

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Pesticide removal in wine with a physical treatment by molecular sieving

All along the winemaking process, conditioning and aging, wine is susceptible to be contaminated by different molecules. Contaminations can have various origins, related to wine microorganisms or as a result of an exogenous contamination. The aforementioned contamination of the wine can be caused by the migration of molecules from the materials in contact with the wine or by a contamination from exogenous molecules present in the air. Regardless of the source of the contamination, mainly two types of consequences can be observed.

Anthropogenic factors in modulations of fungal populations from grapes to wines and their repercussions on wine characteristics

The effects of anthropogenic activities on vineyard (different plant protections) and in winery
(pressing/clarification step, addition of sulfur dioxide) on fungal populations from grape to wine were studied. The studied anthropogenic activities modify the fungal diversity. Thus, lower biodiversity of grapes from organic modality was measured for the three vintages considered compared to biodiversity from ecophyto modality and conventional modality. The pressing / clarification steps strongly modify fungal populations and the influence of the winery flora is highlighted.

Spontaneous fermentation dynamics of indigenous yeast populations and their effect on the sensory properties of Riesling

Varietal Riesling aroma relies strongly on the formation and liberation of bound aroma compounds. Floral monoterpenes, green C6-alcohols, fruity C13-norisoprenoids and spicy volatile phenols are predominantly bound to disaccharides, which are produced and stored in the grape berry during berry maturation. Grape processing aims to extract maximum amount of the precursors from the berry skin to increase the potential for a strong varietal aroma in the wine. Subsequent yeast selection plays an important part in this process.

Comparative proteomic analysis of wines made from Botrytis cinerea infected and healthy grapes reveal interesting parallels to the gushing phenomenon in sparkling wine

In addition to aroma compounds also protein composition strongly influences the quality of wines. Proteins of wine derive mainly from the plant Vitis vinifera and may be influenced by abiotic stress as well as fermentation conditions or fining. Additionally, fungal infections can affect the protein content as well by introducing fungal proteins or affecting grape protein composition. An infection of the vine with the plant pathogenic fungus Botrytis (B.) cinerea was shown to cause a degradation of proteins in the resulting wine. Moreover, it influences the foaming properties in sparkling wine.

Cytochrome P450 CYP71BE5 from grapevine (Vitis vinifera) catalyzes the formation of the spicy aroma compound, (-)-rotundone

(-)-Rotundone, an oxygenated sesquiterpene, is a potent odorant molecule with a characteristic spicy aroma existing in various plants including grapes1. It is considered as a significant compound notably in wines and grapes because of its low sensory threshold (16 ng L-1 in red wine, 8 ng L-1 in water) and aroma properties. (-)-Rotundone was first identified in red wine made from the grape cultivar Syrah (regionally called Shiraz) in Australia1, and then it was found in several grape varieties such as Duras, Grüner Veltliner, Schioppettino and Vespolina from Europe2, 3. Several environmental factors affecting the accumulation of (-)-Rotundone during the grape maturation, were reported such as ambient temperature4, soil properties and topography5, soil moisture from irrigation and light exposure in the bunch zone by leaf removal2.