Macrowine 2021
IVES 9 IVES Conference Series 9 Fining-Derived Allergens in Wine: from Detection to Quantification

Fining-Derived Allergens in Wine: from Detection to Quantification

Abstract

Since 2012, EU Commission approved compulsory labeling of wines treated with allergenic additives or processing aids “if their presence can be detected in the final product” (EU Commission Implementing Regulation No. 579/2012 of 29 June 2012). The list of potential allergens to be indicated on wine labels comprises sulphur dioxide and milk- and egg- derived fining agents, including hen egg lysozyme, which is usually added in wines as preservative. In some non-EU countries, the list includes gluten, tree nuts and fish gelatins. With the exception of lysozyme, all these fining proteins were long thought to be totally removed by subsequent winemaking processings (e.g. bentonite addition). Suitable methods to detect and quantify potential residual allergens are necessary to ensure compliance with legislation. ELISA kits are routinely used to ensure the absence of allergenic proteins in wines, since they are easy to perform. The required LOD and LOQ for milk and egg products with ELISA methods are 0.25 ppm and 0.5 ppm (OIV Comex 502- 2012). In a recent paper (1), no detectable egg white protein residue was found in red wines by ELISA, even when coadiuvant was added at 660 ppm, without following bentonite treatment. In 2011, a clinical survey (2) reported that, although no allergen residue was detected by ELISA kits in the fined wines, positive skin prick test reactions and basophil activation to the treated wines were observed in the majority of patients with allergy to milk, egg or fish, correlating with the concentration of the fining agents used. From these findings, some uncentainty about the safety of wines certified as “allergen-free” through ELISA methods can exist. More recently, mass spectrometry have been proposed, mainly as a confirmatory method of results from ELISA. The two main drawbacks for MS-based methods, especially in the past, were insufficient LOD and LOQ, and high costs. At present, some methods with competitive performance and linearity have been set up for milk and egg protein based fining aids. Aim of the present presentation is to review the different approaches in the detection of allergenic residues in wines, including recent metrological approaches, and development of innovative biosensors. Preliminary data on MS-based method that is currently being developed in our laboratory will be presented, as well future perspectives on the topic and possible implications for consumers health, safety and ethical preferences.

1- Uberti F., et al. Immunochemical investigation of allergenic residues in experimental and commercially-available wines fined with egg white proteins. Food Chem. 2014;159:343-52. 2- Vassilopoulou E., et al. Risk of allergic reactions to wine, in milk, egg and fish-allergic patients. Clin Transl Allergy. 2011;1:10.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Maria Gabriella Giuffrida*, Cristina Lamberti, Daniela Gastaldi, Laura Cavallarin, Marzia Giribaldi

*ISPA

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Effect of intra‐vineyard ripeness variation on the efficiency of commercial enzymes on berry cell wall deconstruction under winemaking conditions

Intra-vineyard variation grape berry ripening occurs within bunches, between bunches on the same vine and between vines. Although it is assumed that such variation also occurs at the grape berry cell wall level, no study to data has investigated in any depth. Here we have used a intra-vineyard panel design to investigate pooled bunches from six vines (per panel) in the context of a winemaking scenario. The dissected vineyard was harvested by separate panels, where each panel was then subjected to a standard winemaking procedure with or without the addition of three different enzyme preparations for maceration.

Testing the effectiveness of Cell-Wall material from grape pomace as fining agent for red wines

Lately several works highlighted the capacity of grape cell-wall material (CWM) to interact with proanthocyanidins (PA), indicating its potential use as fining agent for red wines.1–4 However, those studies were performed by using purified PAs and very high doses of CWM (almost ten-fold higher than those used in wine industry for other commercial fining agents). The present study focuses on the applicability of CWM from Cabernet sauvignon pomace as fining agent for red wines under real winery conditions. Grapes of cultivar Cabernet sauvignon were harvested at three different maturity levels
(unripe, mature, and overripe) and used for red winemaking. The pomace of such vinifications were used as source of CWM, and applied into red wines at two different concentrations: 0.2 g/L and 2.5 g/L.

Oak wood seasoning: impact on oak wood chemical composition and sensory quality of wine

Oak wood selection and maturation are essential steps in the course of barrel fabrication. Given the existence of many factors involved in the choice of raw material and in natural seasoning of oak wood, it is very difficult to determine the real impact of seasoning and selection factors on oak wood composition. A sampling was done to study the evolution of oak wood chemical composition during four seasoning steps: non matured, 12 months, 18 months and 24 months. For this sampling, three selection factors were taken into account: age, grain type and the Polyphenolic Index measured by Oakscan®. Besides extractables
(~10%), three polymers constitute the main part of oak wood: cellulose, hemicelluloses and lignins.

South Africa’s top 10 Sauvignon blanc wines. How do the chemical and sensory profiles compare?

FNB Top 10 Sauvignon Blanc competition, presented by the Sauvignon Blanc Interest Group of South Africa and sponsored by First National Bank, is the country’s foremost platform for producers of this cultivar to showcase and benchmark their wines. Wines entered in the competition originated from all over the winegrowing regions of the country and the winning wines showed good representation of quality South African Sauvignon blanc wines. The ten selected wines were subjected to various chemical analyses including volatile thiol and methoxypyrazine determination, while the sensory profile of each wine was determined using projective mapping.

Evaluation of colloidal stability in white and rosé wines investing Dynamic Light Scattering technology

Proteins constitute one of the three main components of grape juice and white wine, phenolic compounds and polysaccharides being the others. A specific group of the total grape-derived proteins resists degradation or adsorption during the winemaking process and remains in finished wine if not removed by the commonplace commercial practice of bentonite fining. While bentonite is effective in removing the problematic proteins, it is claimed to adversely affect the quality of the treated wine under certain conditions, through the removal of colour, flavor and texture compounds. A number of studies have indicated that different protein fractions require distinct bentonite concentrations for protein removal and consequent heat stabilization.