Macrowine 2021
IVES 9 IVES Conference Series 9 Fining-Derived Allergens in Wine: from Detection to Quantification

Fining-Derived Allergens in Wine: from Detection to Quantification

Abstract

Since 2012, EU Commission approved compulsory labeling of wines treated with allergenic additives or processing aids “if their presence can be detected in the final product” (EU Commission Implementing Regulation No. 579/2012 of 29 June 2012). The list of potential allergens to be indicated on wine labels comprises sulphur dioxide and milk- and egg- derived fining agents, including hen egg lysozyme, which is usually added in wines as preservative. In some non-EU countries, the list includes gluten, tree nuts and fish gelatins. With the exception of lysozyme, all these fining proteins were long thought to be totally removed by subsequent winemaking processings (e.g. bentonite addition). Suitable methods to detect and quantify potential residual allergens are necessary to ensure compliance with legislation. ELISA kits are routinely used to ensure the absence of allergenic proteins in wines, since they are easy to perform. The required LOD and LOQ for milk and egg products with ELISA methods are 0.25 ppm and 0.5 ppm (OIV Comex 502- 2012). In a recent paper (1), no detectable egg white protein residue was found in red wines by ELISA, even when coadiuvant was added at 660 ppm, without following bentonite treatment. In 2011, a clinical survey (2) reported that, although no allergen residue was detected by ELISA kits in the fined wines, positive skin prick test reactions and basophil activation to the treated wines were observed in the majority of patients with allergy to milk, egg or fish, correlating with the concentration of the fining agents used. From these findings, some uncentainty about the safety of wines certified as “allergen-free” through ELISA methods can exist. More recently, mass spectrometry have been proposed, mainly as a confirmatory method of results from ELISA. The two main drawbacks for MS-based methods, especially in the past, were insufficient LOD and LOQ, and high costs. At present, some methods with competitive performance and linearity have been set up for milk and egg protein based fining aids. Aim of the present presentation is to review the different approaches in the detection of allergenic residues in wines, including recent metrological approaches, and development of innovative biosensors. Preliminary data on MS-based method that is currently being developed in our laboratory will be presented, as well future perspectives on the topic and possible implications for consumers health, safety and ethical preferences.

1- Uberti F., et al. Immunochemical investigation of allergenic residues in experimental and commercially-available wines fined with egg white proteins. Food Chem. 2014;159:343-52. 2- Vassilopoulou E., et al. Risk of allergic reactions to wine, in milk, egg and fish-allergic patients. Clin Transl Allergy. 2011;1:10.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Maria Gabriella Giuffrida*, Cristina Lamberti, Daniela Gastaldi, Laura Cavallarin, Marzia Giribaldi

*ISPA

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Effect of supplementation with inactive yeast during alcoholic fermentation in base wine for sparkling

INTRODUCTION: Foam stability of sparkling wines is significantly favored by the presence of surface active agents such as proteins and polysaccharides [1]. For that reason, the renowned sparkling wines are aged after the second fermentation in contact with the lees for several months (even years). Thereby wines are enriched in these macromolecules due to yeast autolysis. Since this practice is slow and costly, winemakers are seeking for alternative procedures to increase their concentration in base wines. In that sense, the supplementation with inactive yeast during alcoholic fermentation has been proposed [2]. The aim of this study was to determine whether this new strategy is really useful for enriching base wines in macromolecules and for improving foam properties of the base wines.

Characterization of free and glycosidically bound simple phenols in hybrid grape varieties using liquid chromatography coupled to high resolution mass (q-orbitrap)

Vitis vinifera is one of the most diffused grapevines over the word and it is the raw material for high quality wines production. The availability of more resistant interspecific hybrid vine varieties, developed from crosses between Vitis vinifera and other Vitis species, has generating much interest, also due to the low environmental effect of production. However, hybrid grape wine composition and varietal differences between interspecific hybrids are not well defined. Different studies revealed that wine consumption has health effects due to its high content of antioxidants, as phenolic compounds. In particular, simple phenols are appreciated not only for their physiological health benefits, including antioxidant, anti-inflammatory and cardioprotective effects, but also because they affect wines organoleptic profile and have a significant role in defining their nutritional characteristics.

Fingerprinting the origin of rosé wines with a new high throughput polyphenomics method

Wine is a widely consumed alcoholic beverage with a high commercial value. More specifically, the worldwide consumption of rosé wine has increased by 20% since 2002[1]. But because of its high commercial value, it can become a subject of fraud, and authenticity control is necessarily required. More than one hundred polyphenols have been recently quantified in various rosé wines [2]. They are key components defining color, taste and quality of wines. Their amount and composition depend on many different factors such as grape variety, winemaking and age of the wine. In this study, the influence of geographic origin of some rosé French wines was investigated. An original and very fast UPLC-QTOF-MS method was developed and used to predict the geographic origin authenticity of rosé wines.

Impact of smoke exposure on the chemical composition of grapes

Vineyard exposure to smoke can lead to grapes and wine which exhibit objectionable smoky and ashy aromas and flavours, more commonly known as ‘smoke taint’ [1, 2]. In the last decade, significant bushfires have occurred around the world, including near wine regions in Australia, Canada, South Africa and the USA, as a consequence of the warmer, drier conditions associated with climate change. Considerable research has subsequently been undertaken to determine the chemical, sensory and physiological consequences of grapevine exposure to smoke. The sensory attributes associated with smoke-tainted wine have been linked to the presence of several smoke-derived volatile phenols, such as guaiacols, syringols and cresols [2].

Analysis of the oenological potentials of different oak forests in Hungary

Like France, Hungary has many oak forests used for making barrels since many years. But if the differences between the woods of the North, the East and the South-West forests of France are well known, this is probably not the case of Hungarian forests. However taking into account the essential differences of climates and soils, differences must be significant and the general name “Hungarian oak” must not have any real meaning. We have studied precisely (determination of concentrations of volatile and non-volatile wood compounds, anatomical criteria, measurement of antioxidant capacity) of oaks collected from northeastern Hungary and others collected from the Danube valley in the northwest of the country.