Macrowine 2021
IVES 9 IVES Conference Series 9 Impact of varying ethanol and carbonation levels on the odor threshold of 1,1,6-trimethyl-1,2-dihydronaphtalene (petrol off-flavor) and role of berry size and Riesling clones

Impact of varying ethanol and carbonation levels on the odor threshold of 1,1,6-trimethyl-1,2-dihydronaphtalene (petrol off-flavor) and role of berry size and Riesling clones

Abstract

1,1,6-trimethyl-1,2-dihydronaphtelene (TDN) evokes the odor of “petrol” in wine, especially in the variety Riesling. Increasing UV-radiation due to climate change intensifies formation of carotenoids in the berry skins and an increase of TDN-precursors1. Exploring new viticultural and oenological strategies to limit TDN formation in the future requires precise knowledge of TDN thresholds in different matrices. Thresholds reported in the literature vary substantially between 2 µg/L up to 20 µg/L2,3,4 due to the use of different methods. As Riesling grapes are used for very different wine styles such as dry, sweet or sparkling wines, it is essential to study the impact of varying ethanol and carbonation levels. Therefore we determined the odor detection threshold (DT) with a three alternatives forced choice (3-AFC) test and calculated the best estimate threshold (BET) for each panelist, followed by the calculation of the BET for the whole panel. Matrices varied from water, a model wine, a dry Riesling wine and sparkling wine; the latter two exceptionally low in TDN. Carbonation in water, model wine and Riesling wine ranged from no addition to 2.5 and 6 bar pressure. Ethanol altered from 8 to 14% alc. in 2% alc. increments. Carbonation yielded an inconsistent effect due to a better volatilization leading to lower thresholds and masking of the TDN perception leading to slightly higher thresholds. Increasing ethanol levels however showed a clear tendency to raise the TDN thresholds, presumably due to better solubility and masking by its own pungent odor. Absolute thresholds varied in water between 2.6 and 4.0 µg/L and in wine between 8.5 and 15.2 µg/L. Since TDN is formed by a breakdown of carotenes, its occurrence correlates positively with the degree of sun exposure. This is partially governed by berry size and cluster density given by clonal differences. Thus we studied free and bound TDN in grapes and wines from 8 different Riesling clones. Furthermore, berries were divided in a two fractions according to a diameter smaller and larger than 13 mm. The berry fractions were separately crushed, pressed and fermented. Preliminary results indicate that the clonal influence on the formation of free TDN in the wines was substantially larger than the impact of berry size.

Literature: 1: Winterhalter, P; Goek, R. 2013; Carotenoid Cleavage Products. ACS Symp. Series 1134, 125-137. 2: Sacks, G. L.; Gates, M. J.; Ferry, F. X.; Lavin, E. H.; Kurtz, A. J.; Acree, T. E. 2012; Journal of Agricultural and Food Chemistry 60(12), 2998-3004 3: Ross, C. F.; Zwink, A. C.; Castro, L.; Harrison, R. 2014; Australian Journal of Grape and Wine Research 20(3), 335-339 4: Simpson, R. F. 1978; Chemistry and Industry 1, 37.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Article

Authors

Michael Ziegler*, Hans-Georg Schmarr, Johanna Molenda, Recep Gök, Sandra Klink, Ulrich Fischer

*DLR Rheinpfalz

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Towards multi-purpose valorisation of polyphenols from grape pomace: Pressurized liquid extraction coupled to purification by membrane processes

Grape by-products (including skins, seeds, stems and vine shoots) are rich in health promoting polyphenols. Their extraction from winery waste and their following purification are of special interest to produce extracts with high added value compounds. Meanwhile, the growing concern over environmental problems associated with economic constraints, require the development of environmentally sustainable extraction technologies. The extraction using semi-continuous subcritical water, as a natural solvent at high temperature and high pressure a technology is promising “green” technology that is environmentally friendly, energy efficient and improve the extraction process in plant tissues.

Interactions of wine polyphenols with dead or living Saccharomyces cerevisiae Yeast Cells and Cell Walls: polyphenol location by microscopy

Tannin, anthocyanins and their reaction products play a major role in the quality of red wines. They contribute to their sensory characteristics, particularly colour and astringency. Grape tannins and anthocyanins are extracted during red wine fermentation. However, their concentration and composition change over time, due to their strong chemical reactivity1. It is also well known that yeasts influence the wine phenolic content, either through the release of metabolites involved in the formation of derived pigments1, or through polyphenol adsorption2,3.

Characterizing the effects of nitrogen on grapevines with different scion/rootstock combinations: agronomic, metabolomic and transcriptomic approaches

Most vineyards are grafted and include a variety (Vitis vinifera) grafted over a wild Vitis rootstock (hybrids of V. berlandieri, riparia and rupestris). Grape berry quality at harvest depends on a subtle balance between acidity and the concentrations of sugars, polyphenols and precursors of aroma compounds. The mechanisms controlling the balance of sugars/acids/polyphenols are influenced by the abiotic environment, in particular nitrogen supply, and interact with the genotypes of both the scion variety and the rootstock. Previous work suggests that some of the effects of water stress are in fact linked to a nitrogen deficiency driven indirectly by the reduction of water absorption.

Impact of drought stress on concentration and composition of wine proteins in Riesling

Protein haze in white wines is a major technological and economic problem of the wine industry. Field tests were carried out in steep slope vineyards planted with Riesling grapes over 3 dry growing seasons to study the effect of drought stress on the concentration of proteins in the resulting wines. Plots suffering from drought stress were compared with surrounding drip irrigated plots. Riesling grapes were processed into wines by conventional procedures. Protein amounts of the isolated wine colloids of the stressed samples were always higher than those of the watered samples(mean watered 13.8 ± 0.44, mean stressed 17.4 ± 0.40 g 100 g-1). As a consequence, higher bentonite doses were needed to achieve protein haze stability of the drought stressed treatments.

Metabolomics comparison of non-Saccharomyces yeasts in Sauvignon blanc and Shiraz

Saccharomyces cerevisiae (SC) is the main driver of alcoholic fermentation however, in wine, non-Saccharomyces species can have a powerful effect on aroma and flavor formation. This study aimed to compare untargeted volatile compound profiles from SPME-GC×GC-TOF-MS of Sauvignon blanc and Shiraz wine inoculated with six different non-Saccharomyces yeasts followed by SC. Torulaspora delbrueckii (TD), Lachancea thermotolerans (LT), Pichia kluyveri (PK) and Metschnikowia pulcherrima (MP) were commercial starter strains, while Candida zemplinina (CZ) and Kazachstania aerobia (KA), were isolated from wine grape environments. Each fermentation produced a distinct chemical profile that was unique for both grape musts. The SC-monoculture and CZ-SC sequential fermentations were the most distinctly different in the Sauvignon blanc while the LT-SC sequential fermentations were the most different from the control in the Shiraz fermentations.