Macrowine 2021
IVES 9 IVES Conference Series 9 Impact of varying ethanol and carbonation levels on the odor threshold of 1,1,6-trimethyl-1,2-dihydronaphtalene (petrol off-flavor) and role of berry size and Riesling clones

Impact of varying ethanol and carbonation levels on the odor threshold of 1,1,6-trimethyl-1,2-dihydronaphtalene (petrol off-flavor) and role of berry size and Riesling clones

Abstract

1,1,6-trimethyl-1,2-dihydronaphtelene (TDN) evokes the odor of “petrol” in wine, especially in the variety Riesling. Increasing UV-radiation due to climate change intensifies formation of carotenoids in the berry skins and an increase of TDN-precursors1. Exploring new viticultural and oenological strategies to limit TDN formation in the future requires precise knowledge of TDN thresholds in different matrices. Thresholds reported in the literature vary substantially between 2 µg/L up to 20 µg/L2,3,4 due to the use of different methods. As Riesling grapes are used for very different wine styles such as dry, sweet or sparkling wines, it is essential to study the impact of varying ethanol and carbonation levels. Therefore we determined the odor detection threshold (DT) with a three alternatives forced choice (3-AFC) test and calculated the best estimate threshold (BET) for each panelist, followed by the calculation of the BET for the whole panel. Matrices varied from water, a model wine, a dry Riesling wine and sparkling wine; the latter two exceptionally low in TDN. Carbonation in water, model wine and Riesling wine ranged from no addition to 2.5 and 6 bar pressure. Ethanol altered from 8 to 14% alc. in 2% alc. increments. Carbonation yielded an inconsistent effect due to a better volatilization leading to lower thresholds and masking of the TDN perception leading to slightly higher thresholds. Increasing ethanol levels however showed a clear tendency to raise the TDN thresholds, presumably due to better solubility and masking by its own pungent odor. Absolute thresholds varied in water between 2.6 and 4.0 µg/L and in wine between 8.5 and 15.2 µg/L. Since TDN is formed by a breakdown of carotenes, its occurrence correlates positively with the degree of sun exposure. This is partially governed by berry size and cluster density given by clonal differences. Thus we studied free and bound TDN in grapes and wines from 8 different Riesling clones. Furthermore, berries were divided in a two fractions according to a diameter smaller and larger than 13 mm. The berry fractions were separately crushed, pressed and fermented. Preliminary results indicate that the clonal influence on the formation of free TDN in the wines was substantially larger than the impact of berry size.

Literature: 1: Winterhalter, P; Goek, R. 2013; Carotenoid Cleavage Products. ACS Symp. Series 1134, 125-137. 2: Sacks, G. L.; Gates, M. J.; Ferry, F. X.; Lavin, E. H.; Kurtz, A. J.; Acree, T. E. 2012; Journal of Agricultural and Food Chemistry 60(12), 2998-3004 3: Ross, C. F.; Zwink, A. C.; Castro, L.; Harrison, R. 2014; Australian Journal of Grape and Wine Research 20(3), 335-339 4: Simpson, R. F. 1978; Chemistry and Industry 1, 37.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Article

Authors

Michael Ziegler*, Hans-Georg Schmarr, Johanna Molenda, Recep Gök, Sandra Klink, Ulrich Fischer

*DLR Rheinpfalz

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Maturation of Agiorgitiko (Vitis vinifera) red wine on its wine lees: Impact on its phenolic composition

Maturation of wine on lees (often referred as sur lie) is a common practice applied by many winemakers around the world. In the past this method was applied mainly on white and/or sparkling wine production but recently also to red wine production. In our experiment, we matured red wine on wine lees of two origins: a) Light wine lees, collected after the completion of the alcoholic fermentation, b) Heavy lees, collected after the completion of the malolactic fermentation. The lees were free of off-odors and were added in the red wine in percentage 3% and 8%, simulating common winemaking addition. The maturation lasted in total six months and samples were collected for analysis after one, three and six months. During storage the lees were stirred.

Comparison of aroma-related compounds of carbonic maceration and traditional young red winemaking in case of Merlot by means of targeted metabolomic approach

Winemaking decisions and techniques are known to affect the final aromatic composition of red wines. Winemakers put a constant effort into the improved controlling of vinification procedures to achieve better quality. Anyway an increased customer’s demand for uniqueness is often forcing them to adjust and offer new and new interesting products. To support the producers, an improved knowledge on aromatic potential as affected by classical and alternative strategies is needed.

Field-grown Sauvignon Blanc berries react to increased exposure by controlling antioxidant homeostasis and displaying UV acclimation responses that are influenced by the level of ambient light

Leaf removal in the bunch zone is a common viticultural practice with several objectives, yet it has been difficult to conclusively link the physiological mechanism(s) and metabolic berry impact to this widely practiced treatment. We used a field-omics approach1 in a Sauvignon blanc high altitude model vineyard, showing that the early leaf removal in the bunch zone caused quantifiable and stable responses (over years) in the microclimate where the main perturbation was increased exposure. We provide an explanation for how leaf removal leads to the shifts in grape metabolites typically linked to this treatment and confirm anecdotal evidence and previous reports that leaf removal treatment at an early stage of berry development affects “quality-associated” metabolites (monoterpenes and norisoprenoids).

Evidence for terroir effect associated with botrytisation relatively to compounds implicated in typical aromas of noble rot sweet wines

Recent studies have demonstrated the role of certain lactones, particularly 2-nonen-4-olide, and volatile thiols (3-sulfanylhexan-1-ol) in the over ripped aromas of noble rot sweet wines (Stamatopoulos et al. 2014ab). These compounds are partly formed during the maturation and under the activity of B. cinerea on grapes. This research was carried out in the vineyard of Sauternes with aim to better understand their genesis depending on the grape over-ripening on two different soil types during 3 vintages. Thus, the study was conducted, with the Sémillon grape, during vintages 2012, 2014 & 2015, at 4 stages of over-maturation of the grapes (healthy, pourri plein, pourri roti, pourri roti + 15 days) considering two vineyard plots with different soil characteristics (calcosol & peyrosol) planted with the 315 Sémillon clone and grafted on 101-14 rootstock respectively in 1981 and 1980 and cultivated with the same vineyard management. Volatile lactones were assayed by liquid-liquid extraction followed by GC/MS analysis and the precursors of 3-sulfanylhexanol by an adaptation of the method by Capone et al. 2010 (SPE-
UPLC/FTMS).

Identification of caffeic acid as a major component of Moscatel wine protein sediment

Proteins play a significant role in the colloidal stability and clarity of white wines [1]. However, under conditions of high temperatures during storage or transportation, the proteins themselves can self-aggregate into light-dispersing particles causing the so-called protein haze [2]. Formation of these unattractive precipitates in bottled wine is a common defect of commercial wines, making them unacceptable for sale [3]. Previous studies identified the presence of phenolic compounds in the natural precipitate of white wine [4], contributing to the hypothesis that these compounds could be involved in the mechanism of protein haze formation.