Macrowine 2021
IVES 9 IVES Conference Series 9 Reduction of herbaceous aromas by wine lactic acid bacteria mediated degradation of volatile aldehydes

Reduction of herbaceous aromas by wine lactic acid bacteria mediated degradation of volatile aldehydes

Abstract

Consumers typically prefer wines with floral and fruity aromas over those presenting green-pepper, vegetal or herbaceous notes. Pyrazines have been identified as causatives for herbaceous notes in wines, especially Bordeaux reds. However, pyrazines are not universally responsible for herbaceousness, and several other wine volatile compounds are known to produce distinct vegetal/herbaceous aromas in wines. Specifically, volatile aldehydes elicit sensations of herbaceousness or grassiness and have been described in wines well above their perception thresholds. Acetaldehyde is quantitatively the most important aldehyde and formed by yeast metabolism or through the auto-oxidation of ethanol during and after fermentations. Its grassy-green aroma typically is prevented by addition of SO2 that strongly binds to acetaldehyde hence masking its aroma. Hetero- and homofermentative wine lactic acid bacteria are responsible for the secondary malolactic fermentation in most red and some white wines and can degrade acetaldehyde. During malolactic fermentation, wine lactic acid bacteria are capable of reducing acetaldehyde levels significantly (~90%). Two reaction pathways were previously described by our group, the chemical reduction of acetaldehyde to ethanol by alcohol dehydrogenase (ADH), or its oxidation to acetic acid by aldehyde dehydrogenase (Al-DH). ADH and Al-DH are known to have a broad substrate specificity. Hence, it is possible that wine lactic acid bacteria may be able to degrade other volatile aldehydes that are known to contribute to herbaceousness in wines. Hexanal, methional, 2-methylbutanal, 3-methylbutanal, 2-methylpropanal, E-2-nonenal and phenyl-acetaldehyde are aldehydes and powerful herbaceous aroma compounds with odour thresholds between 0.5 and 16 µg/l. The odour thresholds of their corresponding alcohols are 100 to 14’000 times higher. Thus, chemical reduction of these aldehydes to the corresponding alcohols by wine lactic acid bacteria may lead to a reduction of herbaceous notes. Within the scope of this investigation, highly concentrated solutions of resting cells of several heterofermentative and facultative homofermentative wine lactic acid bacteria of the genera Oenococcus and Lactobacillus were tested for their ability to degrade these volatile aldehydes. A careful incubation and sample-taking protocol was applied in order to prevent sample evaporation. The analysis of volatile aldehydes was performed by liquid-liquid micro-extraction followed by GC-MS analysis. It could be demonstrated that all bacteria were able to degrade all volatile aldehydes efficiently. Within 50 minutes, an average of 95% of the initial aldehyde concentration was degraded with minima and maxima of 63 and 100%, respectively. The results suggest that wine lactic acid bacteria may be able to degrade volatile aldehydes during malolactic fermentation thus reducing their sensory impact and increasing sensory perception of compounds with fruity character.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Ramon Mira de Orduna*, Alexandra Le Boursier, Marilyn Cléroux, Tatevik Gabrielyan

*HES-SO

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

WineMetrics: A new approach to unveil the “wine-like aroma” chemical feature

“The Human being has an excellent ability to detect and discriminate odors but typically has great difficulty in identifying specific odorants”(1). Furthermore, “from a cognitive point of view the mechanism used to judge wines is closer to pattern recognition than descriptive analysis.” Therefore, when one wants to reveal the volatile “wine-like feature” pattern recognition techniques are required. Sensomics is one of the most recent “omics”, i.e. a holistic perspective of a complex system, which deals with the description of substances originated from microorganism metabolism that are “active” to human senses (2). Depicting the relevant volatile fraction in wines has been an ongoing task in recent decades to which several research groups have allocated important resources. The most common strategy has been the “target approach” in order to identify the “key odorants” for a given wine varietal.

Development of a new sustainable filtering media for wine and beer clarification and sterilisation

Different separation techniques are frequently used during vinification process. Nowadays, clarification and microbiological stabilization of wine or beer can be done using precoat filters or crossflow filters to remove yeast and bacteria. Kieselguhr powders are the most used filter aids for precoat filtration. Their crystalline structure and their pulverulent nature induce ecotoxicological risks when used. Moreover, regeneration and reuse of these filter aids is not efficient and the filtration waste requires cost effective retreatment.

Oenological features of Sangiovese wine from vinification of whole grape berries

The present study was performed in a traditional winery located in the viticultural area of Brunello di Montalcino, Siena, Italy, in the vintage 2015. Actually, in this winery Sangiovese grape musts are fermented in large oak barrels by a single strain of Saccharomyces cerevisiae previously isolated in the same winery. Pumping over operations are carried out once or twice a day until the end of alcoholic fermentations. The aim of this work was to investigate on the oenological properties of Sangiovese wine produced with the traditional winemaking process adopted by the winery under study obtained from the fermentation of whole berries compared to that from crushed grape must. In particular, two lots of 65q of Sangiovese grapes from the same 3ha vineyard were vinified in 150hL oak barrels.

Evaluating South African Chenin blanc wine styles using an LC-MS screening method

Sorting Chenin blanc is one of the most important white wine cultivars in South Africa. It has received a lot of attention and accolades in the past years and more research than ever is dedicated to this versatile cultivar. According to the Chenin blanc association of South Africa, there are three recognized dry wine styles, Fresh and Fruity (FF), Rich and Ripe Unwooded
(RRU), and Rich and Ripe Wooded (RRW). They are traditionally established with the aid of expert sensory evaluation, but the cost and the (subjective) human factor are aspects to be taken into account. A more objective and possibly robust way of assessing and attributing these styles can be the use of chemical analysis.

Ripening of cv. Cabernet Sauvignon grapes: polysaccharides fractions evolution and phenolic extractability

Polysaccharides and more specifically pectins, make up a significant portion of the cell wall material of the plant cells including the grapes. During the fruit ripening the associated softening is related to the breakdown of the cell wall polysaccharides. During this process, it is expected that polysaccharides that are soluble in red wine will be formed influencing its texture. Anthocyanins are responsible for the wine color and tannins for the astringency, body and bitterness of the wine. In the skins, these compounds are located in the cell vacuoles and the barrier that conditions their extractability is the skin cell wall that may determine the mechanical resistance, the texture and the ease of processing berries. The aim of this work was study the evolution of the polysaccharides and the anthocyanin and tannin extractability during the ripening period in Cabernet Sauvignon grapes, trying to correlate these variables.