Macrowine 2021
IVES 9 IVES Conference Series 9 Reduction of herbaceous aromas by wine lactic acid bacteria mediated degradation of volatile aldehydes

Reduction of herbaceous aromas by wine lactic acid bacteria mediated degradation of volatile aldehydes

Abstract

Consumers typically prefer wines with floral and fruity aromas over those presenting green-pepper, vegetal or herbaceous notes. Pyrazines have been identified as causatives for herbaceous notes in wines, especially Bordeaux reds. However, pyrazines are not universally responsible for herbaceousness, and several other wine volatile compounds are known to produce distinct vegetal/herbaceous aromas in wines. Specifically, volatile aldehydes elicit sensations of herbaceousness or grassiness and have been described in wines well above their perception thresholds. Acetaldehyde is quantitatively the most important aldehyde and formed by yeast metabolism or through the auto-oxidation of ethanol during and after fermentations. Its grassy-green aroma typically is prevented by addition of SO2 that strongly binds to acetaldehyde hence masking its aroma. Hetero- and homofermentative wine lactic acid bacteria are responsible for the secondary malolactic fermentation in most red and some white wines and can degrade acetaldehyde. During malolactic fermentation, wine lactic acid bacteria are capable of reducing acetaldehyde levels significantly (~90%). Two reaction pathways were previously described by our group, the chemical reduction of acetaldehyde to ethanol by alcohol dehydrogenase (ADH), or its oxidation to acetic acid by aldehyde dehydrogenase (Al-DH). ADH and Al-DH are known to have a broad substrate specificity. Hence, it is possible that wine lactic acid bacteria may be able to degrade other volatile aldehydes that are known to contribute to herbaceousness in wines. Hexanal, methional, 2-methylbutanal, 3-methylbutanal, 2-methylpropanal, E-2-nonenal and phenyl-acetaldehyde are aldehydes and powerful herbaceous aroma compounds with odour thresholds between 0.5 and 16 µg/l. The odour thresholds of their corresponding alcohols are 100 to 14’000 times higher. Thus, chemical reduction of these aldehydes to the corresponding alcohols by wine lactic acid bacteria may lead to a reduction of herbaceous notes. Within the scope of this investigation, highly concentrated solutions of resting cells of several heterofermentative and facultative homofermentative wine lactic acid bacteria of the genera Oenococcus and Lactobacillus were tested for their ability to degrade these volatile aldehydes. A careful incubation and sample-taking protocol was applied in order to prevent sample evaporation. The analysis of volatile aldehydes was performed by liquid-liquid micro-extraction followed by GC-MS analysis. It could be demonstrated that all bacteria were able to degrade all volatile aldehydes efficiently. Within 50 minutes, an average of 95% of the initial aldehyde concentration was degraded with minima and maxima of 63 and 100%, respectively. The results suggest that wine lactic acid bacteria may be able to degrade volatile aldehydes during malolactic fermentation thus reducing their sensory impact and increasing sensory perception of compounds with fruity character.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Ramon Mira de Orduna*, Alexandra Le Boursier, Marilyn Cléroux, Tatevik Gabrielyan

*HES-SO

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Technological possibilities of grape marc cell walls as wine fining agent. Effect on wine phenolic composition

Fining is a technique that is used to remove unwanted wine components that affect clarification, astringency, color, bitterness, and aroma. Fining involves the addition of adsorptive or reactive material in order to reduce or eliminate the presence of certain less desirable wine components and to ensure that a wine remains in a particular stable state for a given period of time Recently concerns have been raised about the addition of animal proteins, such as gelatin, to wine due to the disease known as bovine spongiform encephalopathy (Mad Cow disease). Although the origin of gelatins has been moved to porcine, winemakers are asking for substitute products with properties and application protocols similar to the traditional animal-derived ones, making the use of plant-derived proteins in fining a practically viable possibility. As a consequence, various fining agents derived from plants have been proposed, including proteins from cereals, legumes, and potato.

Partial dealcoholisation of red wine by reverse osmosis-evaporative perstraction: impact on wine composition

Around the world, the alcohol content of wine has been steadily increasing; partly as a consequence of climate change, but also due to improvements in viticultural management practices and winemaking techniques [1,2]. Concurrently, market demand for wines with lower alcohol levels has increased as consumers seek to reduce alcohol intake for social and/or health reasons [3]. As such, there is increasing demand for both innovative methods that allow winemakers to produce ‘reduced alcohol wines’ (RAW) and a better understanding of the impact of such methods on the composition of RAW. This study therefore aimed to investigate compositional changes in two red wines resulting from partial alcohol removal following treatment by one such method, involving a combination of reverse osmosis and evaporative perstraction (RO-EP).

Ageing of Sauvignon Blanc white wines with Specific Inactivated Dry Yeasts: Effect on physical and chemical characteristics

Del Barrio-Galán, R.a, b, Gómez-Parrini, A.a, Peña-Neira, A.b a Lallemand Inc. Chile y Compañía Limitada, Rosario Norte 407, piso 6, Las condes, Santiago, Chile b Department of Agro-Industry and Enology, Faculty of Agronomical Sciences, University of Chile, Post Office Box 1004, Santa Rosa 11315, La Pintana, Santiago, Chile It is well known that polysaccharides, mainly mannoproteins, play an important role on physical, chemical and sensory quality of wines. The ageing of white wines on lees is used in order to release higher amounts of polysaccharides by the autolytic processes in order to obtain higher-quality wines. However, this technique is too slow, because the temperature and pH conditions are not the most suitable for this process. In addition, it can also involve certain disadvantages such as a greater demand on winery resources, a longer period of wine storage, the appearance of reduction notes and some microbiological alterations.

Anthocyanin accumulation and extractability during the maturation of the grapes of three varieties

Anthocyanin accumulation and extractability were studied in Tannat, Cabernet Sauvignon and Merlot grapes produced in the south of Uruguay in two consecutive seasons. Typical cultivation situations employed in the region for each variety were considered. A follow-up was carried out, considering 60 plants per vineyard, and the harvest was determined according to the technological indices of maturity. Samples of grapes were taken in duplicate in each vineyard periodically along grape maturation. The basic composition, polyphenolic potential and anthocyanin extractability were determined. Also, half of grapes were frozen and later peeled; skin extractions over 24 hs with a solution of 12% ethanol and pH 3.2 were carried out. The anthocyanin contents of the extracts obtained were determined by HPLC-DAD. The levels of anthocyanins reached the highest values before technological maturity. Anthocyanin extractability had a decrease during grape maturation.

Application of high power ultrasounds during red wine vinification

Wine color is one of the main organoleptic characteristics influencing its quality. It is of especial interest in red vinifications due to the economic resources that wineries have to invest for the extraction of the phenolic compounds responsible of wine color, compounds that are mainly located inside the skin cell vacuoles. Moreover, these phenolic compounds not only influence color but also other organoleptic properties such as body, mouthfeel, astringency and flavour. The transference of phenolic compounds from grapes to must during vinification is closely related with the type of grapes and the winemaking technique.