Macrowine 2021
IVES 9 IVES Conference Series 9 Reduction of herbaceous aromas by wine lactic acid bacteria mediated degradation of volatile aldehydes

Reduction of herbaceous aromas by wine lactic acid bacteria mediated degradation of volatile aldehydes

Abstract

Consumers typically prefer wines with floral and fruity aromas over those presenting green-pepper, vegetal or herbaceous notes. Pyrazines have been identified as causatives for herbaceous notes in wines, especially Bordeaux reds. However, pyrazines are not universally responsible for herbaceousness, and several other wine volatile compounds are known to produce distinct vegetal/herbaceous aromas in wines. Specifically, volatile aldehydes elicit sensations of herbaceousness or grassiness and have been described in wines well above their perception thresholds. Acetaldehyde is quantitatively the most important aldehyde and formed by yeast metabolism or through the auto-oxidation of ethanol during and after fermentations. Its grassy-green aroma typically is prevented by addition of SO2 that strongly binds to acetaldehyde hence masking its aroma. Hetero- and homofermentative wine lactic acid bacteria are responsible for the secondary malolactic fermentation in most red and some white wines and can degrade acetaldehyde. During malolactic fermentation, wine lactic acid bacteria are capable of reducing acetaldehyde levels significantly (~90%). Two reaction pathways were previously described by our group, the chemical reduction of acetaldehyde to ethanol by alcohol dehydrogenase (ADH), or its oxidation to acetic acid by aldehyde dehydrogenase (Al-DH). ADH and Al-DH are known to have a broad substrate specificity. Hence, it is possible that wine lactic acid bacteria may be able to degrade other volatile aldehydes that are known to contribute to herbaceousness in wines. Hexanal, methional, 2-methylbutanal, 3-methylbutanal, 2-methylpropanal, E-2-nonenal and phenyl-acetaldehyde are aldehydes and powerful herbaceous aroma compounds with odour thresholds between 0.5 and 16 µg/l. The odour thresholds of their corresponding alcohols are 100 to 14’000 times higher. Thus, chemical reduction of these aldehydes to the corresponding alcohols by wine lactic acid bacteria may lead to a reduction of herbaceous notes. Within the scope of this investigation, highly concentrated solutions of resting cells of several heterofermentative and facultative homofermentative wine lactic acid bacteria of the genera Oenococcus and Lactobacillus were tested for their ability to degrade these volatile aldehydes. A careful incubation and sample-taking protocol was applied in order to prevent sample evaporation. The analysis of volatile aldehydes was performed by liquid-liquid micro-extraction followed by GC-MS analysis. It could be demonstrated that all bacteria were able to degrade all volatile aldehydes efficiently. Within 50 minutes, an average of 95% of the initial aldehyde concentration was degraded with minima and maxima of 63 and 100%, respectively. The results suggest that wine lactic acid bacteria may be able to degrade volatile aldehydes during malolactic fermentation thus reducing their sensory impact and increasing sensory perception of compounds with fruity character.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Ramon Mira de Orduna*, Alexandra Le Boursier, Marilyn Cléroux, Tatevik Gabrielyan

*HES-SO

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Wood from barrique: release of phenolic compounds and permeability to oxygen

Chemical and sensory changes occurring in red wine during ageing in oak barrique are due to the slow and gradual entrance of oxygen along with a release of ellagic tannin from the wood. Though oxygen can enter the cask through the bunghole, it is not clear the role of permeation through the wood staves as well as the amount of oxygen entering by permeation. The distribution of the released ellagic tannins in the wine ageing is also unknown. The oxygen passing through the bunghole may have a different wine ageing effect compared to the oxygen permeating through the wooden staves owing to the uneven ellagic tannin concentration throughout the wine.

Simultaneous monitoring of dissolved CO2 and collar from Rosé sparkling wine glasses: the impact of yeast macromolecules

Champagne or sparkling wines elaborated through the same traditional method, which consists in two major yeast-fermented steps, typically hold about 10 to 12 g/L of dissolved CO2 after the second fermentation in a closed bottle. Hundreds of molecules and macromolecules originating from grape and yeast cohabit with dissolved CO2; they are essential compounds contributing to many organoleptic characteristics (effervescence, foam, aroma, taste, colour…). Indeed, the second alcoholic fermentation and the maturation on lees (which may last from 12 months up to several years) both induce various quantitative and qualitative changes in the wine through the action of yeast, as listed hereafter: development of aromas during aging on lees, release of nitrogen compounds during autolysis and release of macromolecules (polysaccharides, lipids, nucleic acids) in wine.

Estimation of chemical age of red wines with the use of Fourier transform infrared spectroscopy (FT-IR) and chemometrics

The color of a red wine is one of the most important parameters of its quality, giving much information on its status, such as the grape variety used or the winemaking style. As the result of a complex equilibrium between different forms of anthocyanins and polymerization reactions which occur over the course of time, color can also serve as an indication of a wines’ age. For this purpose the “chemical age” i and ii indexes have been introduced by Somers in 1977. The chemical age index i measures the color absorbance after the addition of acetaldehyde while chemical index ii provides an indication of how much of the total red pigments are resistant to SO2 bleaching.

Fingerprinting the origin of rosé wines with a new high throughput polyphenomics method

Wine is a widely consumed alcoholic beverage with a high commercial value. More specifically, the worldwide consumption of rosé wine has increased by 20% since 2002[1]. But because of its high commercial value, it can become a subject of fraud, and authenticity control is necessarily required. More than one hundred polyphenols have been recently quantified in various rosé wines [2]. They are key components defining color, taste and quality of wines. Their amount and composition depend on many different factors such as grape variety, winemaking and age of the wine. In this study, the influence of geographic origin of some rosé French wines was investigated. An original and very fast UPLC-QTOF-MS method was developed and used to predict the geographic origin authenticity of rosé wines.

Monitoring of Pesticide Residues from Vine to Wine

Those previous years, pesticides are often brought to the forefront by media. Questions arose about their toxicity for growers and consumers. Even if a downward trend is underway, the use of pesticides is required to ensure steady quality and quantity of harvests. A large number of active ingredients are authorized but regarding viticulture, mainly insecticides and fungicides are applied, to control pests and diseases and to increase crop yield. Some phytosanitary products, principally fungicides, applied close to the harvest date may frequently be detected in wines.