Macrowine 2021
IVES 9 IVES Conference Series 9 Reduction of herbaceous aromas by wine lactic acid bacteria mediated degradation of volatile aldehydes

Reduction of herbaceous aromas by wine lactic acid bacteria mediated degradation of volatile aldehydes

Abstract

Consumers typically prefer wines with floral and fruity aromas over those presenting green-pepper, vegetal or herbaceous notes. Pyrazines have been identified as causatives for herbaceous notes in wines, especially Bordeaux reds. However, pyrazines are not universally responsible for herbaceousness, and several other wine volatile compounds are known to produce distinct vegetal/herbaceous aromas in wines. Specifically, volatile aldehydes elicit sensations of herbaceousness or grassiness and have been described in wines well above their perception thresholds. Acetaldehyde is quantitatively the most important aldehyde and formed by yeast metabolism or through the auto-oxidation of ethanol during and after fermentations. Its grassy-green aroma typically is prevented by addition of SO2 that strongly binds to acetaldehyde hence masking its aroma. Hetero- and homofermentative wine lactic acid bacteria are responsible for the secondary malolactic fermentation in most red and some white wines and can degrade acetaldehyde. During malolactic fermentation, wine lactic acid bacteria are capable of reducing acetaldehyde levels significantly (~90%). Two reaction pathways were previously described by our group, the chemical reduction of acetaldehyde to ethanol by alcohol dehydrogenase (ADH), or its oxidation to acetic acid by aldehyde dehydrogenase (Al-DH). ADH and Al-DH are known to have a broad substrate specificity. Hence, it is possible that wine lactic acid bacteria may be able to degrade other volatile aldehydes that are known to contribute to herbaceousness in wines. Hexanal, methional, 2-methylbutanal, 3-methylbutanal, 2-methylpropanal, E-2-nonenal and phenyl-acetaldehyde are aldehydes and powerful herbaceous aroma compounds with odour thresholds between 0.5 and 16 µg/l. The odour thresholds of their corresponding alcohols are 100 to 14’000 times higher. Thus, chemical reduction of these aldehydes to the corresponding alcohols by wine lactic acid bacteria may lead to a reduction of herbaceous notes. Within the scope of this investigation, highly concentrated solutions of resting cells of several heterofermentative and facultative homofermentative wine lactic acid bacteria of the genera Oenococcus and Lactobacillus were tested for their ability to degrade these volatile aldehydes. A careful incubation and sample-taking protocol was applied in order to prevent sample evaporation. The analysis of volatile aldehydes was performed by liquid-liquid micro-extraction followed by GC-MS analysis. It could be demonstrated that all bacteria were able to degrade all volatile aldehydes efficiently. Within 50 minutes, an average of 95% of the initial aldehyde concentration was degraded with minima and maxima of 63 and 100%, respectively. The results suggest that wine lactic acid bacteria may be able to degrade volatile aldehydes during malolactic fermentation thus reducing their sensory impact and increasing sensory perception of compounds with fruity character.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Ramon Mira de Orduna*, Alexandra Le Boursier, Marilyn Cléroux, Tatevik Gabrielyan

*HES-SO

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Sensory impacts of the obturator used for the Chasselas: study over the time

Many parameters affect the organoleptic characteristics of wine: internal parameters like the chemical composition or polyphenol content and external as for example storage conditions or the type of obturator. The aim of this study was to characterize sensorally the impacts of several type of obturator on a white wine: Chasselas. To determine the organoleptic characteristics of this wine, a quantitative descriptive analysis could be used. But rapid sensory methods were preferred in this project. Indeed these methods are an appropriate alternative to conventional descriptive methods for quickly assessing sensory product discrimination.

Identification of green, aggressive and hard character of wines by a chemo-sensory directed methodology

With climate change, it is progressively more often to obtain grapes with an acceptable content in sugars or acids but with immature tannins described as green, aggressive or hard (noted as GAH onwards). During winemaking, the oenologist has to make decisions related to the elaboration of such grapes based mainly on empirical experience, given the lack of objective criteria to this concern. An increase in the chemical and sensory knowledge of immature tannins would allow managing this GAH character of grapes with the maximum possible efficiency during winemaking processes. The present work aims at isolating and identifying the group of compounds responsible for the GAH character present in wines.

The moment of preharvest elicitor application influence its final effect on winegrapes quality

Phenolic compounds are secondary metabolites of grapes. Plants produce a wide variety of this type of metabolites through diverse biosynthesis pathways and their production is sometimes a response to external stimuli, either environmental or biotic stresses. Some of them may act as chemical defenses against pathogens or herbivores and their synthesis is increased when the attack exists. However, it is remarkable that the synthesis of these interesting compounds can be activated even when the stimulus is not present, with the use of elicitors. These are substances that when applied exogenously trigger the biosynthetic pathways conducting to the synthesis of these defense compounds.

A multivariate approach using attenuated total reflectance mid-infrared spectroscopy to measure the surface mannoproteins and β-glucans of yeast cell walls during wine fermentations

Yeast cells possess a cell wall comprising primarily glycoproteins, mannans, and glucan polymers. Several yeast phenotypes relevant for fermentation, wine processing, and wine quality are correlated with cell wall properties. To investigate the effect of wine fermentation on cell wall composition, a study was performed using mid-infrared (MIR) spectroscopy coupled with multivariate methods (i.e., PCA and OPLS-DA). A total of 40 yeast strains were evaluated, including Saccharomyces strains (laboratory and industrial) and non-Saccharomyces species. Cells were fermented in both synthetic MS300 and Chardonnay grape must to stationery phase, processed, and scanned in the MIR spectrum.

Effect of supplementation with inactive yeast during alcoholic fermentation in base wine for sparkling

INTRODUCTION: Foam stability of sparkling wines is significantly favored by the presence of surface active agents such as proteins and polysaccharides [1]. For that reason, the renowned sparkling wines are aged after the second fermentation in contact with the lees for several months (even years). Thereby wines are enriched in these macromolecules due to yeast autolysis. Since this practice is slow and costly, winemakers are seeking for alternative procedures to increase their concentration in base wines. In that sense, the supplementation with inactive yeast during alcoholic fermentation has been proposed [2]. The aim of this study was to determine whether this new strategy is really useful for enriching base wines in macromolecules and for improving foam properties of the base wines.