Macrowine 2021
IVES 9 IVES Conference Series 9 Reduction of herbaceous aromas by wine lactic acid bacteria mediated degradation of volatile aldehydes

Reduction of herbaceous aromas by wine lactic acid bacteria mediated degradation of volatile aldehydes

Abstract

Consumers typically prefer wines with floral and fruity aromas over those presenting green-pepper, vegetal or herbaceous notes. Pyrazines have been identified as causatives for herbaceous notes in wines, especially Bordeaux reds. However, pyrazines are not universally responsible for herbaceousness, and several other wine volatile compounds are known to produce distinct vegetal/herbaceous aromas in wines. Specifically, volatile aldehydes elicit sensations of herbaceousness or grassiness and have been described in wines well above their perception thresholds. Acetaldehyde is quantitatively the most important aldehyde and formed by yeast metabolism or through the auto-oxidation of ethanol during and after fermentations. Its grassy-green aroma typically is prevented by addition of SO2 that strongly binds to acetaldehyde hence masking its aroma. Hetero- and homofermentative wine lactic acid bacteria are responsible for the secondary malolactic fermentation in most red and some white wines and can degrade acetaldehyde. During malolactic fermentation, wine lactic acid bacteria are capable of reducing acetaldehyde levels significantly (~90%). Two reaction pathways were previously described by our group, the chemical reduction of acetaldehyde to ethanol by alcohol dehydrogenase (ADH), or its oxidation to acetic acid by aldehyde dehydrogenase (Al-DH). ADH and Al-DH are known to have a broad substrate specificity. Hence, it is possible that wine lactic acid bacteria may be able to degrade other volatile aldehydes that are known to contribute to herbaceousness in wines. Hexanal, methional, 2-methylbutanal, 3-methylbutanal, 2-methylpropanal, E-2-nonenal and phenyl-acetaldehyde are aldehydes and powerful herbaceous aroma compounds with odour thresholds between 0.5 and 16 µg/l. The odour thresholds of their corresponding alcohols are 100 to 14’000 times higher. Thus, chemical reduction of these aldehydes to the corresponding alcohols by wine lactic acid bacteria may lead to a reduction of herbaceous notes. Within the scope of this investigation, highly concentrated solutions of resting cells of several heterofermentative and facultative homofermentative wine lactic acid bacteria of the genera Oenococcus and Lactobacillus were tested for their ability to degrade these volatile aldehydes. A careful incubation and sample-taking protocol was applied in order to prevent sample evaporation. The analysis of volatile aldehydes was performed by liquid-liquid micro-extraction followed by GC-MS analysis. It could be demonstrated that all bacteria were able to degrade all volatile aldehydes efficiently. Within 50 minutes, an average of 95% of the initial aldehyde concentration was degraded with minima and maxima of 63 and 100%, respectively. The results suggest that wine lactic acid bacteria may be able to degrade volatile aldehydes during malolactic fermentation thus reducing their sensory impact and increasing sensory perception of compounds with fruity character.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Ramon Mira de Orduna*, Alexandra Le Boursier, Marilyn Cléroux, Tatevik Gabrielyan

*HES-SO

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

To a better understanding of the impact of vine nitrogen status on volatile thiols from plot to transcriptome level

Volatile thiols contribute largely to the organoleptic characteristics and typicity of Sauvignon blanc wines. Among this family of odorous compounds, 3-sulfanylhexan-1-ol (3SH) and 4-methyl-4-sulfanylpentan-2-one (4MSP) have a major impact on wine flavor. These thiols are formed during alcoholic fermentation by the yeast from odorless and non-volatile precursors found in the berry and the must. The effect of vine nitrogen status on 3SH and 4MSP in Sauvignon blanc wine and on the glutathionylated and cysteinylated precursors of 3SH (Glut-3SH and Cys-3SH) was investigated in this study.

Analysis of the oenological potentials of different oak forests in Hungary

Like France, Hungary has many oak forests used for making barrels since many years. But if the differences between the woods of the North, the East and the South-West forests of France are well known, this is probably not the case of Hungarian forests. However taking into account the essential differences of climates and soils, differences must be significant and the general name “Hungarian oak” must not have any real meaning. We have studied precisely (determination of concentrations of volatile and non-volatile wood compounds, anatomical criteria, measurement of antioxidant capacity) of oaks collected from northeastern Hungary and others collected from the Danube valley in the northwest of the country.

Bentonite fining in cold wines: prediction tests, reduced efficiency and possibilities to avoid additional fining treatments

Bentonite fining is widely used to prevent protein haze in white wines. Most wineries use laboratory-scale fining trials to define the appropriate amount of bentonite to be used in the cellar. Those pre-tests need to mimic as much as possible the industrial scale fining procedure to determine the exact amount of bentonite necessary for protein stability. Nevertheless it is frequent that, after fining with the recommended amount of bentonite, wines appear still unstable and need an additional fining treatment. It remains a major challenge to understand why the same wine, fined with the same dosage of the same bentonite, achieves stability in the lab, but not in the cellar.

Grape metabolites, aroma precursors and the complexities of wine flavour

A critical aspect of wine quality from a consumer perspective is the overall impression of wine flavour, which is formed by the interplay of volatile aroma compounds, their precursors, and taste and matrix components. Grapes contribute some potent aroma compounds, together with a large pool of non-volatile precursors (e.g. glycoconjugates and amino acid conjugates). Aroma precursors can break down through chemical hydrolysis reactions, or through the action of yeast or enzymes, significantly changing the aroma profile of a wine during winemaking and storage. In addition, glycoconjugates of monoterpenes, norisoprenoids and volatile phenols, together with sulfur-conjugates in wine, provide a reservoir of additional flavour through the in-mouth release of volatiles which may be perceived retro-nasally.

Identification of green, aggressive and hard character of wines by a chemo-sensory directed methodology

With climate change, it is progressively more often to obtain grapes with an acceptable content in sugars or acids but with immature tannins described as green, aggressive or hard (noted as GAH onwards). During winemaking, the oenologist has to make decisions related to the elaboration of such grapes based mainly on empirical experience, given the lack of objective criteria to this concern. An increase in the chemical and sensory knowledge of immature tannins would allow managing this GAH character of grapes with the maximum possible efficiency during winemaking processes. The present work aims at isolating and identifying the group of compounds responsible for the GAH character present in wines.