Macrowine 2021
IVES 9 IVES Conference Series 9 Full automation of oenological fermentations and its application to the processing of must containing high sugar or acetic acid concentrations

Full automation of oenological fermentations and its application to the processing of must containing high sugar or acetic acid concentrations

Abstract

Climate change and harvest date decisions have led to the evolution of must quality over the last decades. Increases in must sugar concentrations are among the most obvious consequences, quantitatively. Saccharomyces cerevisiae is a robust and acid tolerant organism. These properties, its sugar to ethanol conversion rate and ethanol tolerance make it the ideal production organism for wine fermentations. Unfortunately, high sugar concentrations may affect S. cerevisiae and lead to growth inhibition or yeast lysis, and cause sluggish or stuck fermentations. Even sublethal conditions cause a hyperosmotic stress response in S. cerevisiae which leads to increased formation of fermentation by-products, including acetic acid, which may exceed legal limits in some wines. Recently, an innovative fermentation system based on FT-NIR and modern process technology was developed by our group, allowing fully automated alcoholic fermentations. The system provides accurate real-time information about key-fermentation parameters including glucose, fructose, and ethanol concentrations throughout fermentations. This allows carrying out fed-batch fermentations at constant and low sugar concentrations thus reducing the hyperosmotic stress response of S. cerevisiae. In this research project, the automated fed-batch technique was compared to the traditional batch method and applied to the vinification of a white Chasselas (Gutedel) grape must under practical winery conditions. A research grade FT-NIR spectrophotometer with an InGaAs detector and an external transflectance probe was used providing non-destructive and non-diffusion limited in-line measurement of sugars. The population dynamics of Saccharomyces cerevisiae and apiculate yeast were followed throughout the fermentations, and samples were also analyzed for organic acids, glycerol, primary amino acids, ammonia, and aldehydes. The final wines were subjected to discrimination (2/5) and descriptive sensory (free sorting) analyses by a trained expert panel. The fed-batch technique allowed drastically reducing the titer (1-2 orders of magnitude) and impact of apiculate yeast. This lead to significantly different wines that were rated as being free of defects and fruitier by the trained panel. The kinetics of several key-wine compounds also differed considerably. Wine produced with the fed-batch technique contained no acetic acid and significantly reduced acetaldehyde levels. The research demonstrates the potential for the application of the fed-batch technique for high gravity musts, but also for musts with a high microbiological load. The drastic reduction of acetic acid concentrations offers a biological alternative to the membrane technology based reduction of acetic acid in musts and wines.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Ramon Mira de Orduna*, Arnaud Pernet, Charles Frohmann, Danielle Widmer, Jean-Pascal Bourgeois, Julien Richard, Olivier Vorlet

*HES-SO

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Study of the colour and phenolic evolution of three different tannin/anthocyanin ratios over time in a model wine

Phenolic compounds are important quality indicators in red wine. A large number of polyphenols play an important role in wine development, contributing to the colour and the sensory perception of the wines. Anthocyanins are the pigments responsible for the colour in young red wines while tannins are the principal contributors to the bitterness and the astringency of the wines. Wine polyphenols are considered more complex molecules than grape phenolics, due to the enormous number of chemical reactions which take place during the entire winemaking process and storage, forming more stable compounds.

Dissecting the polysaccharide‐rich grape cell wall matrix during the red winemaking process, using high‐throughput and fractionation methods

Limited information is available on grape wall-derived polymeric structure/composition and how this changes during fermentation. Commercial winemaking operations use enzymes that target the polysaccharide-rich polymers of the cell walls of grape tissues to clarify musts and extract pigments during the fermentations. In this study we have assessed changes in polysaccharide composition/ turnover throughout the winemaking process by applying recently developed cell wall profiling approaches to both wine and pomace polysaccharides. The methods included gas chromatography for monosaccharide composition (GC-MS), infra-red (IR) spectroscopy and comprehensive microarray polymer profiling
(CoMPP) using cell wall probes.

South Africa’s top 10 Sauvignon blanc wines. How do the chemical and sensory profiles compare?

FNB Top 10 Sauvignon Blanc competition, presented by the Sauvignon Blanc Interest Group of South Africa and sponsored by First National Bank, is the country’s foremost platform for producers of this cultivar to showcase and benchmark their wines. Wines entered in the competition originated from all over the winegrowing regions of the country and the winning wines showed good representation of quality South African Sauvignon blanc wines. The ten selected wines were subjected to various chemical analyses including volatile thiol and methoxypyrazine determination, while the sensory profile of each wine was determined using projective mapping.

Microbial stabilization of wines using innovative coiled UV-C reactor process: impact on chemical and organoleptic proprieties

For several years, numerous studies aimed at limiting the use of SO2 in wines (thermal treatments, pulsed electric fields, microwaves …). Processes must be able to preserve the organoleptic qualities of wines with low energy consumption. In this context, ultraviolet radiations (UV-C), at 254 nm, are well known for their germicidal proprieties. In order to inactivate microorganisms in grape juice and wine without affecting the quality of the product, efficiency of UV-C treatment process should be optimized.

Impact of industrial-scale serial filtration on macromolecules in red wines

Filtration is a critical step in ensuring the clarity and microbial stability of wine prior to bottling. However the process of filtering potentially reduces red wine quality by removing some of the macromolecules that contribute to the texture of the wine. Commercial red wines, Cabernet Sauvignon (CAS) and Shiraz (SHZ), of two vintages and two grades (premium grade wines from the older vintage: CAS13 and SHZ13; and standard grade wines from a younger vintage: CAS14 and SHZ14) were filtered through industrial-scale commercial filtration units prior to bottling. Samples were taken before and after cross-flow filtration, lenticular filters, 0.65 µm and 0.45 µm pore size nylon membrane filters. The concentration and composition of macromolecules, including tannins and polysaccharides, were measured in all samples as well as particle size distribution and wine colour.