Macrowine 2021
IVES 9 IVES Conference Series 9 Full automation of oenological fermentations and its application to the processing of must containing high sugar or acetic acid concentrations

Full automation of oenological fermentations and its application to the processing of must containing high sugar or acetic acid concentrations

Abstract

Climate change and harvest date decisions have led to the evolution of must quality over the last decades. Increases in must sugar concentrations are among the most obvious consequences, quantitatively. Saccharomyces cerevisiae is a robust and acid tolerant organism. These properties, its sugar to ethanol conversion rate and ethanol tolerance make it the ideal production organism for wine fermentations. Unfortunately, high sugar concentrations may affect S. cerevisiae and lead to growth inhibition or yeast lysis, and cause sluggish or stuck fermentations. Even sublethal conditions cause a hyperosmotic stress response in S. cerevisiae which leads to increased formation of fermentation by-products, including acetic acid, which may exceed legal limits in some wines. Recently, an innovative fermentation system based on FT-NIR and modern process technology was developed by our group, allowing fully automated alcoholic fermentations. The system provides accurate real-time information about key-fermentation parameters including glucose, fructose, and ethanol concentrations throughout fermentations. This allows carrying out fed-batch fermentations at constant and low sugar concentrations thus reducing the hyperosmotic stress response of S. cerevisiae. In this research project, the automated fed-batch technique was compared to the traditional batch method and applied to the vinification of a white Chasselas (Gutedel) grape must under practical winery conditions. A research grade FT-NIR spectrophotometer with an InGaAs detector and an external transflectance probe was used providing non-destructive and non-diffusion limited in-line measurement of sugars. The population dynamics of Saccharomyces cerevisiae and apiculate yeast were followed throughout the fermentations, and samples were also analyzed for organic acids, glycerol, primary amino acids, ammonia, and aldehydes. The final wines were subjected to discrimination (2/5) and descriptive sensory (free sorting) analyses by a trained expert panel. The fed-batch technique allowed drastically reducing the titer (1-2 orders of magnitude) and impact of apiculate yeast. This lead to significantly different wines that were rated as being free of defects and fruitier by the trained panel. The kinetics of several key-wine compounds also differed considerably. Wine produced with the fed-batch technique contained no acetic acid and significantly reduced acetaldehyde levels. The research demonstrates the potential for the application of the fed-batch technique for high gravity musts, but also for musts with a high microbiological load. The drastic reduction of acetic acid concentrations offers a biological alternative to the membrane technology based reduction of acetic acid in musts and wines.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Ramon Mira de Orduna*, Arnaud Pernet, Charles Frohmann, Danielle Widmer, Jean-Pascal Bourgeois, Julien Richard, Olivier Vorlet

*HES-SO

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Maturation of Agiorgitiko (Vitis vinifera) red wine on its wine lees: Impact on its phenolic composition

Maturation of wine on lees (often referred as sur lie) is a common practice applied by many winemakers around the world. In the past this method was applied mainly on white and/or sparkling wine production but recently also to red wine production. In our experiment, we matured red wine on wine lees of two origins: a) Light wine lees, collected after the completion of the alcoholic fermentation, b) Heavy lees, collected after the completion of the malolactic fermentation. The lees were free of off-odors and were added in the red wine in percentage 3% and 8%, simulating common winemaking addition. The maturation lasted in total six months and samples were collected for analysis after one, three and six months. During storage the lees were stirred.

Attractiveness and sweetness of red wines: Synergies between American oak barrels and mannoproteins

In partnership with a Bordeaux property wanting to improve the quality of its second wine, the effects of two factors, American oak barrels and mannoproteins were studied. Their impact on the attractiveness and sweetness of wines were characterized during two successive vintages (2012 and 2013). Vinification took place with a homogeneous batch of Cabernet Sauvignon. The wine was then divided up into various groups of five barrels of French and American oak, new or reused. Analyses of volatile and non-volatile wood compounds were undertaken at four months and eight months of wood ageing, by LC-MS and GC-MS.

Spontaneous fermentation dynamics of indigenous yeast populations and their effect on the sensory properties of Riesling

Varietal Riesling aroma relies strongly on the formation and liberation of bound aroma compounds. Floral monoterpenes, green C6-alcohols, fruity C13-norisoprenoids and spicy volatile phenols are predominantly bound to disaccharides, which are produced and stored in the grape berry during berry maturation. Grape processing aims to extract maximum amount of the precursors from the berry skin to increase the potential for a strong varietal aroma in the wine. Subsequent yeast selection plays an important part in this process.

Intelligent article to control the internal pressure in continue in bottles

An intelligent packaging might, among others, provide information and allow monitoring of the quality of the packed product or its surrounding environment. A recent project on micro-flow wine bottles closed with aluminium screw cap and tightness liner, highlighted the importance of monitoring the internal overpressure continuously, in real-time and at least for 72 hours, since the internal pressure on the tightness liner and the micro-flow are related. Real-time and continuous measurements are not the standard methods of measurement of the overpressure, yet. The most used equipment for the determination of the pressure in wine bottle is the aphrometer, a destructive device that supplies a single value of pressure.

Screening sensory-directed methodology for the selection of non-saccharomyces wine yeasts based on perceived aroma quality

The present work contributes by developing a rapid sensory-directed methodology for the screening and selection of high quality wines with different sensory profiles Therefore, Verdejo and Tempranillo musts were fermented with 50 different yeasts each under controlled laboratory conditions. Resulting samples were firstly categorized according to five levels of quality by a panel of wine professionals (Sáenz-Navajas, Ballester et al. 2013). Higher quality samples were described by flash profiling by a semi-trained panel
(Valentin, Chollet et al. 2012) and most distinctive samples were screened by gas chromatography-olfactometry (GC-O) (López, Aznar et al. 2002).