Macrowine 2021
IVES 9 IVES Conference Series 9 Full automation of oenological fermentations and its application to the processing of must containing high sugar or acetic acid concentrations

Full automation of oenological fermentations and its application to the processing of must containing high sugar or acetic acid concentrations

Abstract

Climate change and harvest date decisions have led to the evolution of must quality over the last decades. Increases in must sugar concentrations are among the most obvious consequences, quantitatively. Saccharomyces cerevisiae is a robust and acid tolerant organism. These properties, its sugar to ethanol conversion rate and ethanol tolerance make it the ideal production organism for wine fermentations. Unfortunately, high sugar concentrations may affect S. cerevisiae and lead to growth inhibition or yeast lysis, and cause sluggish or stuck fermentations. Even sublethal conditions cause a hyperosmotic stress response in S. cerevisiae which leads to increased formation of fermentation by-products, including acetic acid, which may exceed legal limits in some wines. Recently, an innovative fermentation system based on FT-NIR and modern process technology was developed by our group, allowing fully automated alcoholic fermentations. The system provides accurate real-time information about key-fermentation parameters including glucose, fructose, and ethanol concentrations throughout fermentations. This allows carrying out fed-batch fermentations at constant and low sugar concentrations thus reducing the hyperosmotic stress response of S. cerevisiae. In this research project, the automated fed-batch technique was compared to the traditional batch method and applied to the vinification of a white Chasselas (Gutedel) grape must under practical winery conditions. A research grade FT-NIR spectrophotometer with an InGaAs detector and an external transflectance probe was used providing non-destructive and non-diffusion limited in-line measurement of sugars. The population dynamics of Saccharomyces cerevisiae and apiculate yeast were followed throughout the fermentations, and samples were also analyzed for organic acids, glycerol, primary amino acids, ammonia, and aldehydes. The final wines were subjected to discrimination (2/5) and descriptive sensory (free sorting) analyses by a trained expert panel. The fed-batch technique allowed drastically reducing the titer (1-2 orders of magnitude) and impact of apiculate yeast. This lead to significantly different wines that were rated as being free of defects and fruitier by the trained panel. The kinetics of several key-wine compounds also differed considerably. Wine produced with the fed-batch technique contained no acetic acid and significantly reduced acetaldehyde levels. The research demonstrates the potential for the application of the fed-batch technique for high gravity musts, but also for musts with a high microbiological load. The drastic reduction of acetic acid concentrations offers a biological alternative to the membrane technology based reduction of acetic acid in musts and wines.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Ramon Mira de Orduna*, Arnaud Pernet, Charles Frohmann, Danielle Widmer, Jean-Pascal Bourgeois, Julien Richard, Olivier Vorlet

*HES-SO

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Ageing of sweet wines: oxygen evolution according to bung and barrel type

Barrel ageing is a crucial step in the wine process because it allows many changes to the wine as enrichment, colour stabilization, clarification and also a slow oxygenation. Effects of the oak barrel have to be known to prevent oxidation of the wine. The type of bung used during ageing is also a parameter to consider. Ageing sweet wines in barrel is a real challenge. These wines may need some oxygen at the beginning of ageing but they should be protected at the end of their maturation, to avoid oxidation.

How pressing techniques affect must composition and wine quality of Pinot blanc

This study investigates how the sensory profile of Pinot Blanc is affected from different maceration and pressing techniques. Grapes were sourced from four vineyards in the village Tramin in South Tyrol. For the experiment 200 kg of grapes from each vineyard site were hand picked the day before harvest for the commercial winery took place. Grapes were stored over night at 4°C, homogenized and processed in the experimental winery at Laimburg research centre the day after harvest. Four different pressing techniques were applied in duplicates of 100kg each.

Partial dealcoholisation of red wine by reverse osmosis-evaporative perstraction: impact on wine composition

Around the world, the alcohol content of wine has been steadily increasing; partly as a consequence of climate change, but also due to improvements in viticultural management practices and winemaking techniques [1,2]. Concurrently, market demand for wines with lower alcohol levels has increased as consumers seek to reduce alcohol intake for social and/or health reasons [3]. As such, there is increasing demand for both innovative methods that allow winemakers to produce ‘reduced alcohol wines’ (RAW) and a better understanding of the impact of such methods on the composition of RAW. This study therefore aimed to investigate compositional changes in two red wines resulting from partial alcohol removal following treatment by one such method, involving a combination of reverse osmosis and evaporative perstraction (RO-EP).

Identification, quantification and organoleptic impact of « dried fruit » molecular markers in Merlot and Cabernet Sauvignon grapes and in red wines

The aromas found in young Bordeaux red wines made with Merlot and Cabernet Sauvignon suggest a complex mixture of aromas of fresh red fruits such as cherry or blackberry for Merlot, and strawberry or blackcurrant for Cabernet Sauvignon. The aromas of these wines are closely linked with the maturity of the grapes. The climate change that has occurred during the last decade in Bordeaux has induced changes in the ripening conditions of grape berries. It is now widely admitted that over-ripening of the berries during hot and dry summers results in the development of characteristic flavors reminiscent of cooked fruits (fig, prune). The presence of these overriding odors found in both musts and young wines affects the quality and subtlety of the wine flavor and may shorten its shelf life.

WineMetrics: A new approach to unveil the “wine-like aroma” chemical feature

“The Human being has an excellent ability to detect and discriminate odors but typically has great difficulty in identifying specific odorants”(1). Furthermore, “from a cognitive point of view the mechanism used to judge wines is closer to pattern recognition than descriptive analysis.” Therefore, when one wants to reveal the volatile “wine-like feature” pattern recognition techniques are required. Sensomics is one of the most recent “omics”, i.e. a holistic perspective of a complex system, which deals with the description of substances originated from microorganism metabolism that are “active” to human senses (2). Depicting the relevant volatile fraction in wines has been an ongoing task in recent decades to which several research groups have allocated important resources. The most common strategy has been the “target approach” in order to identify the “key odorants” for a given wine varietal.