Macrowine 2021
IVES 9 IVES Conference Series 9 Characterization of non-Saccharomyces yeast and its interaction with Saccharomyces cerevisiae with investigation of fermentation kinetics and aromatic composition

Characterization of non-Saccharomyces yeast and its interaction with Saccharomyces cerevisiae with investigation of fermentation kinetics and aromatic composition

Abstract

There is growing evidence that non-Saccharomyces yeasts can be utilized to enhance wine aroma although little research has been done on most non-Saccharomyces species. This study was designed to genetically and phenotypically characterize two local South African non-Saccharomyces species, Kazachstania aerobia and Wickerhamomyces anomalus, in addition to determining their fermentation potential and volatile profiles in synthetic grape must. Genetic differences between isolates were investigated using the RAPD method and phenotypic heterogeneity was determined using plate spotting. Isolates were assessed for heat, alcohol, saline, osmotic and oxidative stress tolerance for phenotypic strain characterization. Eight K. aerobia and thirteen W. anomalus isolates were used to ferment synthetic grape must. After characterization three K. aerobia strains and two W. anomalus strains were then selected for the co-culture fermentations with S. cerevisiae VIN13 and EC1118. Fermentations were done by inoculating yeast simultaneously as well as sequentially, 48 hours apart. Single culture fermentations were used as controls. Aroma compounds in the synthetic wine were quantified using GC-FID. RAPD analysis classified W. anomalus isolates into four distinct strains in accordance to place of origin. Phenotypic variations were also evident in the proposed strains’ resistance to oxidative, saline and osmotic stresses compared to VIN13. Interestingly, there were phenotypic differences observed within the same strain groupings. The K. aerobia isolates showed no marked genetic differences, but with slight variations in stress response. Overall, the CBS strain had a higher growth performance than the other strains with K. aerobia Y965 showing the least growth. In co-inoculation experiments of VIN13 and K. aerobia, the latter persisted until day 9 when VIN13 was introduced on day 2 and until day 7 when VIN13 was introduced on day 0 regardless of isolate’s phenotype. When fermenting with EC1118, W. anomalus had higher cell densities compared to when fermented with VIN13. In sequential fermentations W. anomalus survived until day 9 (when fermenting with strain Y934-C) and day 7 (when fermenting with strain LO632). When inoculating simultaneously with both strains of S. cerevisiae, W. anomalus was detected in the must until day five. Kazachstania aerobia and W. anomalus gives a unique aroma profile to wines. Although as single cultures these yeast do not ferment wines to dryness, they are capable of conferring favourable wine aroma when in association S. cerevisiae strains with no risk of sluggish fermentation. Inoculating S. cerevisiae sequentially to the non-Saccharomyces yeast allows sufficient time for the non-Saccharomyces to impart valuable aroma compounds. This study provides a basis for further work on wine quality improvement through exploitation of non-Saccharomyces yeasts.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Judy Lombard*, Florian Bauer, Hannibal Musarurwa, Sandra du Toit

*IWBT

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Red wine substituted esters involved in fruity aromatic expression: an enantiomeric approach to understand their sensory impact and their pathway formation

Among red wines ethyl esters, those from short hydroxylated and branched-chain aliphatic acids constitute a family with a particular behavior and sensory importance. They have been previously discussed in the literature [1] and recent studies have established that some of them were strongly involved in of red wines’ fruity aroma [2]. As some among them have an asymmetrical carbon atom, it seemed important to separate their different enantiomers to obtain an accurate assessment of their organoleptic impact. Three chiral esters have been identified, presenting alkyl and/or hydroxyle substituants: ethyl 2-hydroxy-4-methylpentanoate, ethyl 2-methylbutanoate, and ethyl 3-hydroxybutanoate.

How pressing techniques affect must composition and wine quality of Pinot blanc

This study investigates how the sensory profile of Pinot Blanc is affected from different maceration and pressing techniques. Grapes were sourced from four vineyards in the village Tramin in South Tyrol. For the experiment 200 kg of grapes from each vineyard site were hand picked the day before harvest for the commercial winery took place. Grapes were stored over night at 4°C, homogenized and processed in the experimental winery at Laimburg research centre the day after harvest. Four different pressing techniques were applied in duplicates of 100kg each.

Foam characteristics of white, rosé and red sparkling wines elaborated by the champenoise method

Contribution Foam is the characteristic that differentiates sparkling wines from still wines, being the first sensory attribute that tasters and consumers perceive and that determines the final quality of sparkling wines [1]. The foaming properties mainly depend on the chemical composition of wines [2-3], and different factors involved in wine composition will have an effect on foam quality. In Spain, the sparkling wine market focuses on the production of white and rosé sparkling wine, with very low production of red sparkling wines. However, this type of wines is elaborated in countries like Australia, South-Africa, Argentina, Italy or Portugal, with a great acceptance by consumers. No studies on the foaming characteristics of red sparkling wines have been found.

Testing the effectiveness of Cell-Wall material from grape pomace as fining agent for red wines

Lately several works highlighted the capacity of grape cell-wall material (CWM) to interact with proanthocyanidins (PA), indicating its potential use as fining agent for red wines.1–4 However, those studies were performed by using purified PAs and very high doses of CWM (almost ten-fold higher than those used in wine industry for other commercial fining agents). The present study focuses on the applicability of CWM from Cabernet sauvignon pomace as fining agent for red wines under real winery conditions. Grapes of cultivar Cabernet sauvignon were harvested at three different maturity levels
(unripe, mature, and overripe) and used for red winemaking. The pomace of such vinifications were used as source of CWM, and applied into red wines at two different concentrations: 0.2 g/L and 2.5 g/L.

Impact of non-fruity compounds on red wines fruity aromatic expression: the role of higher alcohols

A part, at least, of the fruity aroma of red wines is the consequence of perceptive interactions between various aromatic compounds, particularly ethyl esters and acetates, which may contribute to the perception of fruity aromas, specifically thanks to synergistic effects.1,2 The question of the indirect impact of non-fruity compounds on this particular aromatic expression has not yet been widely investigated. Among these compounds higher alcohols (HA) represent the main group, from a quantitative standpoint, of volatiles in many alcoholic beverages. Moreover, some bibliographic data suggested their contribution to the aromatic complexity by either increasing or masking flavors of wine, depending of their concentrations.