Macrowine 2021
IVES 9 IVES Conference Series 9 Characterization of non-Saccharomyces yeast and its interaction with Saccharomyces cerevisiae with investigation of fermentation kinetics and aromatic composition

Characterization of non-Saccharomyces yeast and its interaction with Saccharomyces cerevisiae with investigation of fermentation kinetics and aromatic composition

Abstract

There is growing evidence that non-Saccharomyces yeasts can be utilized to enhance wine aroma although little research has been done on most non-Saccharomyces species. This study was designed to genetically and phenotypically characterize two local South African non-Saccharomyces species, Kazachstania aerobia and Wickerhamomyces anomalus, in addition to determining their fermentation potential and volatile profiles in synthetic grape must. Genetic differences between isolates were investigated using the RAPD method and phenotypic heterogeneity was determined using plate spotting. Isolates were assessed for heat, alcohol, saline, osmotic and oxidative stress tolerance for phenotypic strain characterization. Eight K. aerobia and thirteen W. anomalus isolates were used to ferment synthetic grape must. After characterization three K. aerobia strains and two W. anomalus strains were then selected for the co-culture fermentations with S. cerevisiae VIN13 and EC1118. Fermentations were done by inoculating yeast simultaneously as well as sequentially, 48 hours apart. Single culture fermentations were used as controls. Aroma compounds in the synthetic wine were quantified using GC-FID. RAPD analysis classified W. anomalus isolates into four distinct strains in accordance to place of origin. Phenotypic variations were also evident in the proposed strains’ resistance to oxidative, saline and osmotic stresses compared to VIN13. Interestingly, there were phenotypic differences observed within the same strain groupings. The K. aerobia isolates showed no marked genetic differences, but with slight variations in stress response. Overall, the CBS strain had a higher growth performance than the other strains with K. aerobia Y965 showing the least growth. In co-inoculation experiments of VIN13 and K. aerobia, the latter persisted until day 9 when VIN13 was introduced on day 2 and until day 7 when VIN13 was introduced on day 0 regardless of isolate’s phenotype. When fermenting with EC1118, W. anomalus had higher cell densities compared to when fermented with VIN13. In sequential fermentations W. anomalus survived until day 9 (when fermenting with strain Y934-C) and day 7 (when fermenting with strain LO632). When inoculating simultaneously with both strains of S. cerevisiae, W. anomalus was detected in the must until day five. Kazachstania aerobia and W. anomalus gives a unique aroma profile to wines. Although as single cultures these yeast do not ferment wines to dryness, they are capable of conferring favourable wine aroma when in association S. cerevisiae strains with no risk of sluggish fermentation. Inoculating S. cerevisiae sequentially to the non-Saccharomyces yeast allows sufficient time for the non-Saccharomyces to impart valuable aroma compounds. This study provides a basis for further work on wine quality improvement through exploitation of non-Saccharomyces yeasts.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Judy Lombard*, Florian Bauer, Hannibal Musarurwa, Sandra du Toit

*IWBT

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Measurements of the oxygen dissolved in white wines elaborated in barrels without to open the bung of the barrels

Bases on oxoluminescence, we have developed an innovative device for measuring dissolved oxygen in wines in barrels without opening the bung. This system is directly inserted into the wood during the barrel elaboration and can be positioned at different locations of the barrel (the head, the hull …). During two successive vintages we have used this device notably to follow the oxygen dissolved of whites wines elaborated in barrels. This allowed us initially to monitor the oxygen levels of the harvest to bottling the whole elaboration process in barrels of white wines without using techniques of measurement suitable to modify the real values in wines (opening the bung to plunge an oximeter).

Analysis of peptide fraction from white wines

Among nitrogen compounds included in white wines, the peptide fraction is certainly the least studied, however this fraction is quantitatively the most important (Feuillat, 1974). Existing studies concern the fraction below 1 kDa and only for white and sparkling wines (Bartolomé et al, 1997, Desportes et al 2000). In this report, we have developed methods to isolate peptides from reference white wines. Then, we have applied this methodology with bitter wine to answer a research question: is there a relation between peptides and the bitterness of white wine as for some cheese for example (Furtado, 1984)?

The impact of different yeasts and harvest time on the wine quality of Beihong and Beimei (<I>V. vinifera x V. amurensis</I>)

Beihong and Beimei are two wine cultivars from ‘Muscat Hamberg’ (V. vinifera L.) and wild V. amurensis Rupr., which were released in China in 2008. Here,two enology practices were reported. Firstly, the impact of different yeasts including D254, GRE, K1, D21 and BDX on dry wine quality of Beihong and Beimei was investigated. For Beihong, among wines fermented by all yeasts, residual sugar content was the lowest, total anthocyanin and resveratrol contents were the highest in the wine by D254. However, the wine by D254 had lower titrable acid than those by the other yeasts except BDX.

Study of the content of amino acids and biogenic amines in sparkling red wines

The production of red sparkling wines is lower in Spain in comparison with the winemaking of white or rosé sparkling wines. In red sparkling wine processing it is essential to obtain suitable base wines that should have moderate alcohol content, high acidity, good color values, an adequate mouth-feel and a sweet tannin. Grapes for sparkling wine production have to be harvested at low maturity stages, with lower alcohol contents and higher acidities, which will that the phenolic maturity of the grapes is also low, showing green tannins. This paper analyses different treatments in order to minimize these inconveniences: cold maceration-prefermentation and delestage to elaborate the grapes with lower maturity, must nanofiltration, and the partial osmosis of the wines made from grapes with an adequate maturity degree.

Comprehensive exploration of wine aroma-related compounds as promoted by alternative vinification procedures in case of Zelen (Vitis vinifera L.) grapes processing

Not only vintner’s decisions in the vineyard, but also winemaker’s choices of technology approaches in the cellar play a significant role in the final wine style and quality. Whereas traditional technologies within chosen terroir are quite well explored and thus somehow predictable, there is no proper knowledge available on possible outcomes in case of implementing novel, alternative winemaking strategies. To reveal their effects on wine aroma compounds and sensory characteristics, two alternative strategies
(cryoextraction or addition of whole grape berries during last stages of fermentation) were compared to classical Vipava valley winemaking approach as normally used for an autochthonous variety Zelen. After separate vinification and bottling, all the experimental wines were subjected to semiquantitative metabolic profiling of volatile compounds (VOCs) by means of GC/MS and were then also sensorialy evaluated by pre-trained panel.