Macrowine 2021
IVES 9 IVES Conference Series 9 Characterization of non-Saccharomyces yeast and its interaction with Saccharomyces cerevisiae with investigation of fermentation kinetics and aromatic composition

Characterization of non-Saccharomyces yeast and its interaction with Saccharomyces cerevisiae with investigation of fermentation kinetics and aromatic composition

Abstract

There is growing evidence that non-Saccharomyces yeasts can be utilized to enhance wine aroma although little research has been done on most non-Saccharomyces species. This study was designed to genetically and phenotypically characterize two local South African non-Saccharomyces species, Kazachstania aerobia and Wickerhamomyces anomalus, in addition to determining their fermentation potential and volatile profiles in synthetic grape must. Genetic differences between isolates were investigated using the RAPD method and phenotypic heterogeneity was determined using plate spotting. Isolates were assessed for heat, alcohol, saline, osmotic and oxidative stress tolerance for phenotypic strain characterization. Eight K. aerobia and thirteen W. anomalus isolates were used to ferment synthetic grape must. After characterization three K. aerobia strains and two W. anomalus strains were then selected for the co-culture fermentations with S. cerevisiae VIN13 and EC1118. Fermentations were done by inoculating yeast simultaneously as well as sequentially, 48 hours apart. Single culture fermentations were used as controls. Aroma compounds in the synthetic wine were quantified using GC-FID. RAPD analysis classified W. anomalus isolates into four distinct strains in accordance to place of origin. Phenotypic variations were also evident in the proposed strains’ resistance to oxidative, saline and osmotic stresses compared to VIN13. Interestingly, there were phenotypic differences observed within the same strain groupings. The K. aerobia isolates showed no marked genetic differences, but with slight variations in stress response. Overall, the CBS strain had a higher growth performance than the other strains with K. aerobia Y965 showing the least growth. In co-inoculation experiments of VIN13 and K. aerobia, the latter persisted until day 9 when VIN13 was introduced on day 2 and until day 7 when VIN13 was introduced on day 0 regardless of isolate’s phenotype. When fermenting with EC1118, W. anomalus had higher cell densities compared to when fermented with VIN13. In sequential fermentations W. anomalus survived until day 9 (when fermenting with strain Y934-C) and day 7 (when fermenting with strain LO632). When inoculating simultaneously with both strains of S. cerevisiae, W. anomalus was detected in the must until day five. Kazachstania aerobia and W. anomalus gives a unique aroma profile to wines. Although as single cultures these yeast do not ferment wines to dryness, they are capable of conferring favourable wine aroma when in association S. cerevisiae strains with no risk of sluggish fermentation. Inoculating S. cerevisiae sequentially to the non-Saccharomyces yeast allows sufficient time for the non-Saccharomyces to impart valuable aroma compounds. This study provides a basis for further work on wine quality improvement through exploitation of non-Saccharomyces yeasts.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Judy Lombard*, Florian Bauer, Hannibal Musarurwa, Sandra du Toit

*IWBT

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Using elicitors in different grape varieties. Effect over their phenolic composition

Phenolic compounds are very important in crop plants and have been the subject of a large number of studies. Three main reasons can be cited for optimizing the level of phenolic compounds in crop plants: their physiological role in plants, their technological significance for food processing, and their nutritional characteristics1 Indeed, an enormous diversity of phenolic antioxidants is found in fruits and vegetables, and their presence and roles can be affected or modified by several pre- and postharvest cultural practices and/or food processing technologies (Ruiz-García et al. 2012, Goldman et al. 1999, Tudela et al. 2002). In winegrapes, the technological importance of phenolic compounds, mainly flavonoids, is well-known.

Impact of glutathione and elemental sulphur juice addition on the volatile thiol production in South African Sauvignon blanc wine

Three compounds, 3-mercaptohexanol (3MH), 3-mercaptohexyl-acetate (3MHA) and 4-mercapto-4-methylpentan-2-one (4MMP), also known as varietal thiols, have been identified to contribute positively to wine aroma and are responsible for the distinct gooseberry, grapefruit, guava and box tree character found in Sauvignon blanc wines. Certain volatile thiol compounds though, can cause off-aromas of onion, garlic, rubber and rotten egg, this group of molecules is known as reductive sulphur compounds (RSC). This study looks into how the addition of sulphur-compounds to Sauvignon blanc juice contributes to the varietal thiol (3MH and 3MHA) concentration and reductive sulphur compound concentration in South African Sauvignon blanc wine.

Impact of smoke exposure on the chemical composition of grapes

Vineyard exposure to smoke can lead to grapes and wine which exhibit objectionable smoky and ashy aromas and flavours, more commonly known as ‘smoke taint’ [1, 2]. In the last decade, significant bushfires have occurred around the world, including near wine regions in Australia, Canada, South Africa and the USA, as a consequence of the warmer, drier conditions associated with climate change. Considerable research has subsequently been undertaken to determine the chemical, sensory and physiological consequences of grapevine exposure to smoke. The sensory attributes associated with smoke-tainted wine have been linked to the presence of several smoke-derived volatile phenols, such as guaiacols, syringols and cresols [2].

Effect of post-harvest ozone treatments on the skin phenolic composition and extractability of red winegrapes cv Nebbiolo and Barbera

Wine industry is looking forward for innovative, safe and eco-friendly antimicrobial products allowing the reduction of chemical treatments in the grape defense and the winemaking process that can affect negatively the quality of the product. Ozone has been tested in food industry giving good results in preventing fungi and bacteria growth on a wide spectrum of vegetables and fruits, due to its oxidant activity and ability to attack numerous cellular constituents. Ozone leaves no chemical residues on the food surface, decomposing itself rapidly in oxygen. Gaseous ozone has been already tested for table grapes storage and on wine grapes during withering.

Multivariate strategies for red wines classification using stilbenes and flavonols content

Bioactive polyphenols from grapes and wines, like stilbenes and flavonols (SaF), are often determined to nutritional evaluation, but also for many other purposes. The objective of this study was to quantify SaF in red wines from “Campanha Gaúcha”, a large and young viticultural region from South Brazil. Moreover, through statistical analysis, evaluate the influence of these compounds according to varieties, production process, harvest years and micro-regions of cultivation. A total of 58 samples of red wines were analyzed by high-performance liquid chromatography coupled to diode array detector (HPLC-DAD) for determination of trans-resveratrol (R), quercetin (Q), myricetin (M), kaempferol (K), trans-e-viniferin (V) and their precursor, cinnamic acid (C).