Macrowine 2021
IVES 9 IVES Conference Series 9 Modulating role of SO2 in white wine protein haze formation

Modulating role of SO2 in white wine protein haze formation

Abstract

Despite the extensive research performed during the last decades, the multifactorial mechanism responsible for the white wine protein haze formation is not fully characterized. Herein, a new model is proposed, which is based on the experimental identification of sulfur dioxide as a major modulating factor inducing wine protein haze upon heating. As opposed to other reducing agents, such as 2-mercaptoethanol, dithiothreitol and tris(2-carboxyethyl)phosphine hydrochloride (TCEP), the addition of SO2 to must/wine upon heating cleaves intraprotein disulfide bonds, hinders thiol-disulfide exchange during protein interactions and can lead to the formation of novel inter/intraprotein disulfide bonds. Those are eventually responsible for wine protein aggregation which follows a nucleation-growth kinetic model as shown by dynamic light scattering [1]. Protein aggregates were further studied following heat stress to induce aggregation. We were able to dissolve the aggregates in buffer A (8 M urea, 200 mM NaCl and 30 mM sodium citrate pH 3) and B (4 % SDS, 200 mM NaCl and sodium citrate pH 3). Size-exclusion chromatography (SEC) of the dissolved proteins aggregates allowed the characterization of the different species present in solution under reducing and non-reducing conditions. Determination of free sulfhydryl groups present in native and stressed protein was also performed using 5,5ʹ-dithiobis(2-nitrobenzoic acid) (DTNB). We suggest/demonstrate that protein aggregation due to SO2 modulation under wine model solution occurs as a result of the combination between both hydrophobic interactions and the formation of new interprotein disulfide bonds. DTNB assay revealed that there were no free sulfhydryl groups both in native, heat stressed and heat stressed in the presence of SO2. Future work will focus on the study of the different protein aggregate species and on new methods for wine protein stabilization.

[1] Chagas, R., Ferreira, L. M., Laia, C. A., Monteiro, S. & Ferreira, R. B. (2016). The challenging SO2-mediated chemical build-up of protein aggregates in wines. Food Chemistry, 192, 460-469.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Ricardo Chagas*, César Laia, Luísa Carvalho, Ricardo Ferreira, Sara Monteiro

*FCT/UNL

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Evaluation of colloidal stability in white and rosé wines investing Dynamic Light Scattering technology

Proteins constitute one of the three main components of grape juice and white wine, phenolic compounds and polysaccharides being the others. A specific group of the total grape-derived proteins resists degradation or adsorption during the winemaking process and remains in finished wine if not removed by the commonplace commercial practice of bentonite fining. While bentonite is effective in removing the problematic proteins, it is claimed to adversely affect the quality of the treated wine under certain conditions, through the removal of colour, flavor and texture compounds. A number of studies have indicated that different protein fractions require distinct bentonite concentrations for protein removal and consequent heat stabilization.

Oligosaccharides in red wines: could their structure and composition be influenced by the grape-growing

Oligosaccharides have only recently been characterized in wine, and the information on composition and content is still limited. In wine, these molecules are mainly natural byproducts of the degradation of grape berry cell wall polysaccharides. Wine oligosaccharides present several physicochemical properties, being one relevant factor linked to the astringency perception of wines (1,2). A terroir can be defined as a grouping of homogeneous environmental units based on the typicality of the products obtained. This notion is particularly associated with wine, being the climate and the soil two of the major elements of terroir concept.

How pressing techniques affect must composition and wine quality of Pinot blanc

This study investigates how the sensory profile of Pinot Blanc is affected from different maceration and pressing techniques. Grapes were sourced from four vineyards in the village Tramin in South Tyrol. For the experiment 200 kg of grapes from each vineyard site were hand picked the day before harvest for the commercial winery took place. Grapes were stored over night at 4°C, homogenized and processed in the experimental winery at Laimburg research centre the day after harvest. Four different pressing techniques were applied in duplicates of 100kg each.

Towards multi-purpose valorisation of polyphenols from grape pomace: Pressurized liquid extraction coupled to purification by membrane processes

Grape by-products (including skins, seeds, stems and vine shoots) are rich in health promoting polyphenols. Their extraction from winery waste and their following purification are of special interest to produce extracts with high added value compounds. Meanwhile, the growing concern over environmental problems associated with economic constraints, require the development of environmentally sustainable extraction technologies. The extraction using semi-continuous subcritical water, as a natural solvent at high temperature and high pressure a technology is promising “green” technology that is environmentally friendly, energy efficient and improve the extraction process in plant tissues.

Effect of concentration and competition between different fungicide residues on the adsorption efficiency of activated vegetal fibres for treatment of wine

Vineyards are strongly exposed to fungal diseases, attacks from insects and competition with weeds. Most treatments used on grape vines contain synthetic active substances, which may be transferred to the wine. Such pesticides have a negative image because many active substances are potential health hazards. A specific oenological treatment allowing the reduction of pesticide residues in wine based on activated vegetable fibres (AVF) is under examination by the International Organisation for Vine and Wine. This technique works efficiently and alters the wine only little (Lempereur et al. 2014).