Macrowine 2021
IVES 9 IVES Conference Series 9 Analysis of peptide fraction from white wines

Analysis of peptide fraction from white wines

Abstract

Among nitrogen compounds included in white wines, the peptide fraction is certainly the least studied, however this fraction is quantitatively the most important (Feuillat, 1974). Existing studies concern the fraction below 1 kDa and only for white and sparkling wines (Bartolomé et al, 1997, Desportes et al 2000). In this report, we have developed methods to isolate peptides from reference white wines. Then, we have applied this methodology with bitter wine to answer a research question: is there a relation between peptides and the bitterness of white wine as for some cheese for example (Furtado, 1984)? First, after splitting reference wines by means of tangential ultrafiltration we got 3 different fractions: proteins above 10 kDa, peptides between 3 and 10 kDa and small peptides and free amino acids below 3 kDa. The amount of total nitrogen for each fraction was quantified by method of Kjedhal. We confirm that peptides represent the largest fraction of the nitrogen compounds in white wine. We expanded the range of molecular weight and studied the peptide fraction between 1 kDa and 10 kDa. This fraction of interest obtained by tangential ultrafiltration was diafiltrated against water and was concentrated by lyophilization. After, extracts from this fraction was separated by gel exclusion chromatography with the superdex 30 specific for peptides. Each fraction was read by absorbance at the 275 nm and then specifically detected by fluorescence with o-phtalaldehyde (OPA) to differentiate peptides from other molecules like polyphenols which are also detected at this wavelength. This isolation strategy was subsequently applied to white wines more or less bitter to investigate a potential relation between the peptides and the bitter taste. We obtained different peptide profiles between the most and least bitter wine for peptides corresponding to a high molecular weight. Every white wines studied here have similar peptide profiles made of two pools of different peptides. For the bitterest wine, the first pool corresponding to the higher molecular weight is greater. Thus, we may have revealed a relation between a class of peptides and the bitterness of these white wines.

REFERENCE LIST • Bartolomé, B., Moreno-Arribas, V., Pueyo, E., Polo, M.C. (1997) – On-line HPLCL photodiode array detection and derivatization for partial identification of small peptides from white wine. J. Agric. Food Chem. 45, 3374-3381. • Desportes, C., Charpentier, M.,Duteurtre, B. Maujean, A., Duchiron, F. (2000) – Liquid chromatographic fractionation of small peptides from wine. Journal of chromatography A. 893, pages 281-291. • Feuillat, M. (1974) – Contribution à l’étude des composés azotés dans les moûts de raisin et dans les vins. Thèse de Doctorat, université de Dijon. • Furtado, M.M. (1984) – Prevention of bitter taste in cheeses. Bulletin de la fédération Internationale de Laiterie. 177, 113-122.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Francois-Xavier Sauvage*, Caty Chabalier

*INRA

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

An excessive leaf-fruit ratio reduces the yeast assimilable nitrogen in the must

Yeast assimilable nitrogen (YAN) in the grape must is a key variable for wine quality as a source of aroma precursors. In a situation of YAN deficiency, a foliar urea application upon the vine at veraison enhances YAN concentration and facilitates must fermentation. In 2013, Agroscope investigated the impact of leaf-fruit ratio on the nitrogen (N) assimilation and partitioning in grapevine Vitis vinifera cv. Chasselas following foliar-urea application with the aim of improving its efficiency on the YAN concentration.

Impact of drought stress on concentration and composition of wine proteins in Riesling

Protein haze in white wines is a major technological and economic problem of the wine industry. Field tests were carried out in steep slope vineyards planted with Riesling grapes over 3 dry growing seasons to study the effect of drought stress on the concentration of proteins in the resulting wines. Plots suffering from drought stress were compared with surrounding drip irrigated plots. Riesling grapes were processed into wines by conventional procedures. Protein amounts of the isolated wine colloids of the stressed samples were always higher than those of the watered samples(mean watered 13.8 ± 0.44, mean stressed 17.4 ± 0.40 g 100 g-1). As a consequence, higher bentonite doses were needed to achieve protein haze stability of the drought stressed treatments.

Moscatel vine-shoot extracts as grapevine biostimulant to increase the varietal aroma of Airén wines

There is a growing interest in the exploitation of vine-shoots waste, since they are often left or burned. Sánchez-Gómez et al. [1] have shown that vines-shoots aqueous extracts have significant contents of bioactive compounds, among which several polyphenols and volatiles are highlighted. Recent studied had demonstrated that the chemical composition of vine-shoots is enhanced when vine-shoots are toasted
[2,3]. The application of vegetable products in the vineyards has led to significant changes towards a more “Sustainable Viticulture”. An innovative foliar application for Airén vine-shoot extracts have been carried out to the vineyard. It has been shown that they act as grape biostimulants, improving certain wine quality characteristics [4].

How do different oak treatment affect the sensory composition of Chenin blanc wines over time?

Wooden barrels have been the preferred method for oak maturation for wines, but the use of alternative oak products, such as staves and oak chips have increased in South Africa due to lower production costs. This study investigated the effect of different oak products used during fermentation and ageing on the sensory profile, degree of liking and perceived quality of a South African Chenin blanc wine. The different wine treatments included an unoaked tank control wine, wines matured in 5th fill barrels, wines matured in new barrels from three different cooperages, and wines matured in 5th fill barrels with stave inserts from two different cooperages.

Analysis of the oenological potentials of different oak forests in Hungary

Like France, Hungary has many oak forests used for making barrels since many years. But if the differences between the woods of the North, the East and the South-West forests of France are well known, this is probably not the case of Hungarian forests. However taking into account the essential differences of climates and soils, differences must be significant and the general name “Hungarian oak” must not have any real meaning. We have studied precisely (determination of concentrations of volatile and non-volatile wood compounds, anatomical criteria, measurement of antioxidant capacity) of oaks collected from northeastern Hungary and others collected from the Danube valley in the northwest of the country.