Macrowine 2021
IVES 9 IVES Conference Series 9 Analysis of peptide fraction from white wines

Analysis of peptide fraction from white wines

Abstract

Among nitrogen compounds included in white wines, the peptide fraction is certainly the least studied, however this fraction is quantitatively the most important (Feuillat, 1974). Existing studies concern the fraction below 1 kDa and only for white and sparkling wines (Bartolomé et al, 1997, Desportes et al 2000). In this report, we have developed methods to isolate peptides from reference white wines. Then, we have applied this methodology with bitter wine to answer a research question: is there a relation between peptides and the bitterness of white wine as for some cheese for example (Furtado, 1984)? First, after splitting reference wines by means of tangential ultrafiltration we got 3 different fractions: proteins above 10 kDa, peptides between 3 and 10 kDa and small peptides and free amino acids below 3 kDa. The amount of total nitrogen for each fraction was quantified by method of Kjedhal. We confirm that peptides represent the largest fraction of the nitrogen compounds in white wine. We expanded the range of molecular weight and studied the peptide fraction between 1 kDa and 10 kDa. This fraction of interest obtained by tangential ultrafiltration was diafiltrated against water and was concentrated by lyophilization. After, extracts from this fraction was separated by gel exclusion chromatography with the superdex 30 specific for peptides. Each fraction was read by absorbance at the 275 nm and then specifically detected by fluorescence with o-phtalaldehyde (OPA) to differentiate peptides from other molecules like polyphenols which are also detected at this wavelength. This isolation strategy was subsequently applied to white wines more or less bitter to investigate a potential relation between the peptides and the bitter taste. We obtained different peptide profiles between the most and least bitter wine for peptides corresponding to a high molecular weight. Every white wines studied here have similar peptide profiles made of two pools of different peptides. For the bitterest wine, the first pool corresponding to the higher molecular weight is greater. Thus, we may have revealed a relation between a class of peptides and the bitterness of these white wines.

REFERENCE LIST • Bartolomé, B., Moreno-Arribas, V., Pueyo, E., Polo, M.C. (1997) – On-line HPLCL photodiode array detection and derivatization for partial identification of small peptides from white wine. J. Agric. Food Chem. 45, 3374-3381. • Desportes, C., Charpentier, M.,Duteurtre, B. Maujean, A., Duchiron, F. (2000) – Liquid chromatographic fractionation of small peptides from wine. Journal of chromatography A. 893, pages 281-291. • Feuillat, M. (1974) – Contribution à l’étude des composés azotés dans les moûts de raisin et dans les vins. Thèse de Doctorat, université de Dijon. • Furtado, M.M. (1984) – Prevention of bitter taste in cheeses. Bulletin de la fédération Internationale de Laiterie. 177, 113-122.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Francois-Xavier Sauvage*, Caty Chabalier

*INRA

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

How small amounts of oxygen introduced during bottling and storage can influence the metabolic fingerprint and SO2 content of white wines

The impact of minute amounts of headspace oxygen on the post-bottling development of wine is generally considered to be very important, since oxygen, packaging and storage conditions can either damage or improve wine quality. This is reflected in the generalised use of inert bottling lines, where the headspace between the white wine and the stopper is filled with an inert gas. This experiment aimed to address some open questions about the chemistry of the interaction between wine and oxygen, crucial for decisions regarding optimal closure. While it is known that similar amounts of oxygen affect different wines to a variable extent, our knowledge of chemistry is not sufficient to construct a predictive method.

Identification, quantification and organoleptic impact of « dried fruit » molecular markers in Merlot and Cabernet Sauvignon grapes and in red wines

The aromas found in young Bordeaux red wines made with Merlot and Cabernet Sauvignon suggest a complex mixture of aromas of fresh red fruits such as cherry or blackberry for Merlot, and strawberry or blackcurrant for Cabernet Sauvignon. The aromas of these wines are closely linked with the maturity of the grapes. The climate change that has occurred during the last decade in Bordeaux has induced changes in the ripening conditions of grape berries. It is now widely admitted that over-ripening of the berries during hot and dry summers results in the development of characteristic flavors reminiscent of cooked fruits (fig, prune). The presence of these overriding odors found in both musts and young wines affects the quality and subtlety of the wine flavor and may shorten its shelf life.

Using elicitors in different grape varieties. Effect over their phenolic composition

Phenolic compounds are very important in crop plants and have been the subject of a large number of studies. Three main reasons can be cited for optimizing the level of phenolic compounds in crop plants: their physiological role in plants, their technological significance for food processing, and their nutritional characteristics1 Indeed, an enormous diversity of phenolic antioxidants is found in fruits and vegetables, and their presence and roles can be affected or modified by several pre- and postharvest cultural practices and/or food processing technologies (Ruiz-García et al. 2012, Goldman et al. 1999, Tudela et al. 2002). In winegrapes, the technological importance of phenolic compounds, mainly flavonoids, is well-known.

Effect of malolactic fermentation in barrels or stainless steel tanks on wine composition. Influence of the barrel toasting

Ellagitannin, anthocyanin and woody volatile composition of Cabernet Sauvignon wines aged in oak barrels for 12 months was evaluated. Depending on the container where malolactic fermentation (MLF) was carried out, two wine modalities were investigated: wines with MLF carried out in stainless steel tanks and barrel-fermented wines. Three toasting methods (medium toast, MT; medium toast with watering, MTAA; noisette) were considered for ageing of each wine modality. Sensory analyses (triangle and rating tests) were also performed. Two-way ANOVA of the raw experimental data revealed that the toasting method and the container where MLF took place, as well as the interaction between both factors, have a significant influence (p < 0.05) on ellagitannin, anthocyanin and woody volatile profiles of Cabernet Sauvignon wines.

Multivariate strategies for red wines classification using stilbenes and flavonols content

Bioactive polyphenols from grapes and wines, like stilbenes and flavonols (SaF), are often determined to nutritional evaluation, but also for many other purposes. The objective of this study was to quantify SaF in red wines from “Campanha Gaúcha”, a large and young viticultural region from South Brazil. Moreover, through statistical analysis, evaluate the influence of these compounds according to varieties, production process, harvest years and micro-regions of cultivation. A total of 58 samples of red wines were analyzed by high-performance liquid chromatography coupled to diode array detector (HPLC-DAD) for determination of trans-resveratrol (R), quercetin (Q), myricetin (M), kaempferol (K), trans-e-viniferin (V) and their precursor, cinnamic acid (C).