Macrowine 2021
IVES 9 IVES Conference Series 9 Analysis of peptide fraction from white wines

Analysis of peptide fraction from white wines

Abstract

Among nitrogen compounds included in white wines, the peptide fraction is certainly the least studied, however this fraction is quantitatively the most important (Feuillat, 1974). Existing studies concern the fraction below 1 kDa and only for white and sparkling wines (Bartolomé et al, 1997, Desportes et al 2000). In this report, we have developed methods to isolate peptides from reference white wines. Then, we have applied this methodology with bitter wine to answer a research question: is there a relation between peptides and the bitterness of white wine as for some cheese for example (Furtado, 1984)? First, after splitting reference wines by means of tangential ultrafiltration we got 3 different fractions: proteins above 10 kDa, peptides between 3 and 10 kDa and small peptides and free amino acids below 3 kDa. The amount of total nitrogen for each fraction was quantified by method of Kjedhal. We confirm that peptides represent the largest fraction of the nitrogen compounds in white wine. We expanded the range of molecular weight and studied the peptide fraction between 1 kDa and 10 kDa. This fraction of interest obtained by tangential ultrafiltration was diafiltrated against water and was concentrated by lyophilization. After, extracts from this fraction was separated by gel exclusion chromatography with the superdex 30 specific for peptides. Each fraction was read by absorbance at the 275 nm and then specifically detected by fluorescence with o-phtalaldehyde (OPA) to differentiate peptides from other molecules like polyphenols which are also detected at this wavelength. This isolation strategy was subsequently applied to white wines more or less bitter to investigate a potential relation between the peptides and the bitter taste. We obtained different peptide profiles between the most and least bitter wine for peptides corresponding to a high molecular weight. Every white wines studied here have similar peptide profiles made of two pools of different peptides. For the bitterest wine, the first pool corresponding to the higher molecular weight is greater. Thus, we may have revealed a relation between a class of peptides and the bitterness of these white wines.

REFERENCE LIST • Bartolomé, B., Moreno-Arribas, V., Pueyo, E., Polo, M.C. (1997) – On-line HPLCL photodiode array detection and derivatization for partial identification of small peptides from white wine. J. Agric. Food Chem. 45, 3374-3381. • Desportes, C., Charpentier, M.,Duteurtre, B. Maujean, A., Duchiron, F. (2000) – Liquid chromatographic fractionation of small peptides from wine. Journal of chromatography A. 893, pages 281-291. • Feuillat, M. (1974) – Contribution à l’étude des composés azotés dans les moûts de raisin et dans les vins. Thèse de Doctorat, université de Dijon. • Furtado, M.M. (1984) – Prevention of bitter taste in cheeses. Bulletin de la fédération Internationale de Laiterie. 177, 113-122.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Francois-Xavier Sauvage*, Caty Chabalier

*INRA

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Effect of mixed Torulaspora delbrueckii-Saccharomyces cerevisiae culture on rose quality wine

Alcoholic fermentation using no Saccharomyces wine is an effective means of modulating wine aroma. This study investigated the impact of coinoculating Torulaspora delbruecki with two Saccharomyces cerevisiae commercial yeast (QA23, Lallemand; Red Fruit, Sepsa-Enartis) on enological quality parameters, volatile composition and sensory analysis. The following assays were performed on Tempranillo variety: Saccharomyces QA23 (CTQA), Saccharomyces Red Fruit (CTRF), coinoculated T. delbrueckii + S.cerevisiae QA23 (CIQA) and coinoculated T. delbrueckii + S.cerevisiae (CIRF).

Multivariate strategies for red wines classification using stilbenes and flavonols content

Bioactive polyphenols from grapes and wines, like stilbenes and flavonols (SaF), are often determined to nutritional evaluation, but also for many other purposes. The objective of this study was to quantify SaF in red wines from “Campanha Gaúcha”, a large and young viticultural region from South Brazil. Moreover, through statistical analysis, evaluate the influence of these compounds according to varieties, production process, harvest years and micro-regions of cultivation. A total of 58 samples of red wines were analyzed by high-performance liquid chromatography coupled to diode array detector (HPLC-DAD) for determination of trans-resveratrol (R), quercetin (Q), myricetin (M), kaempferol (K), trans-e-viniferin (V) and their precursor, cinnamic acid (C).

HEAT BERRY : Sensitivity of berries ripening to higher temperature and impact on phenolic compounds in wine

The grapevine is an important economical crop that is very sensitive to climate changes and microclimate. The observations made during the last decades at a vineyard scale all concur to show the impact of climate change on vine physiology, resulting in accelerated phenology and earlier harvest (Jones and Davis 2000). It is well-known that berry content is affected by the ambient temperature. While the first experiences were primarily conducted on the impact of temperature on anthocyanin accumulation in the grape, few studies have focused on others component of phenolic metabolism, such as tannins.

Ellagitannins and flavano-ellagitannins: concentration ranges in different areas and sensory evaluation

C-Glucosidic ellagitannins, which are the main polyphenolic compounds in oak heartwood, are extracted by wine during aging in oak barrels. Although such maturing of alcoholic beverages in oak barrels is a multi-centennial practice, very little is known on the impact of these ellagitannins on the organoleptic properties of red wine. The objectives of the present investigation were (i) to isolate oak ellagitannins and to hemisynthesize some made-in-wine flavano-ellagitannins, such as acutissimin A; (ii) to analyse their concentration ranges depending on the cultivar area and (iii) to evaluate their sensory impact on the basis of their human threshold concentrations and dose/response relationships in different types of solutions.

Characterization of Glycosidically Bound Aroma Compounds of País cv. grapes of different Chilean zones

País grape has been estimated to arrive to Chile almost 500 years ago, being the first strain grown in this country. Traditionally, this grape has been used to mix with other varieties, to produce poor quality wines, but today is beginning to be used in the production of high quality wines. However, very little is known about the chemical characteristics of this variety. The aroma is one of the most important quality attributes of wine. Volatile compounds of this beverage may come from the grape (varietal aromas), from the fermentation process, from the ageing. The aromatic compounds are found in the grape in two forms: as free volatile compounds and as non-volatile compounds. The last ones, are aroma precursors present mainly as glycoconjugates formed by a sugar and an aglycone…