Macrowine 2021
IVES 9 IVES Conference Series 9 New biological tools to control and secure malolactic fermentation in high pH wines

New biological tools to control and secure malolactic fermentation in high pH wines

Abstract

Originally, the role of the malolactic fermentation (MLF) was simply to improve the microbial stability of wine via biological deacidification. However, there is an accumulation of evidence to support the fact that lactic acid bacteria (LAB) also contribute positively to the taste and aroma of wine. Many different LAB enter into grape juice and wine from the surface of grape berries, cluster stems, vine leaves, soil and winery equipment. Due to the highly selective environment of juices and wine, only a few types of LAB are able to grow. Wine pH is most selective, and at pH below 3.5 generally only strains of Oenococcus oeni can survive and express malolactic activity, while wines with pH above 3.5 can contain various species of Pediococcus, as well as strains of Lactobacillus. The trend toward harvesting higher maturity grapes has resulted in the processing of higher pH musts and the production of wines containing increased levels of alcohol. These conditions favor the growth of indigenous bacteria and often O. oeni does not prevail at the end of alcoholic fermentation. More Lactobacillus sp. predominate and are often responsible for spontaneous MLF (du Toit et al. 2011). Some L. plantarum strains can tolerate the high alcohol concentrations and SO2 levels normally encountered in wine. Due to their very complex and diverse metabolism a range of compositional changes can be induced, which may affect the quality of the final product positively or negatively. A recent isolate have shown most interesting results, not only for its capacity to induce MLF after direct inoculation in freeze-dried form, but also for their positive contribution to the wine aroma. Co-inoculation (inoculation of selected wine LAB 24 hours after the yeast) can ensure the early implantation and dominance of the selected strain, the early onset and completion of MLF, and can possibly prevent the appearance of the spoilage yeast and bacteria. Applying an important L. plantarum inoculum with high malolactic activity assures an immediate dominance, as well as predictable and complete MLF in short time and allows an early stabilization of the wine. Since it degrades hexose sugars by the homo-fermentative pathway, which poses no risk of acetic acid production from the residual sugars that may be present in high pH wines, it is an interesting alternative to control MLF in high pH wines.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Sibylle Dr. Krieger-Weber*, Anthony Silvano, Magali Deleris-Bou

*Lallemand SAS

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

How pressing techniques affect must composition and wine quality of Pinot blanc

This study investigates how the sensory profile of Pinot Blanc is affected from different maceration and pressing techniques. Grapes were sourced from four vineyards in the village Tramin in South Tyrol. For the experiment 200 kg of grapes from each vineyard site were hand picked the day before harvest for the commercial winery took place. Grapes were stored over night at 4°C, homogenized and processed in the experimental winery at Laimburg research centre the day after harvest. Four different pressing techniques were applied in duplicates of 100kg each.

Comparison of various storage conditions to preserve polyphenols in red-grape pomace

Red grape pomace, a waste from wine production, can be valorised by extracting polyphenols, high-added value compounds used in cosmetics or oenology. For use at an industrial level, using green extraction techniques, pomace need to be stored before being processed. The aim of this study is to test various storage conditions in order to maintain high level of polyphenols over 180 days, while keeping storage cost economically interesting. In a first step, different storage conditions (ambient temperature or cooled (4°C) temperature, anaerobic (saturation with N2) or aerobic conditions, and addition of sulphur dioxide (SO2)) were compared on small samples (1 kg) packed in plastic pockets. The quality of storage was assessed by following the optical density of the pomace extract at 280 nm (DO 280 expressed as mg/l eq gallic acid), which is an indication of the amount of remaining extractable polyphenols.

Ellagitannins and flavano-ellagitannins: concentration ranges in different areas and sensory evaluation

C-Glucosidic ellagitannins, which are the main polyphenolic compounds in oak heartwood, are extracted by wine during aging in oak barrels. Although such maturing of alcoholic beverages in oak barrels is a multi-centennial practice, very little is known on the impact of these ellagitannins on the organoleptic properties of red wine. The objectives of the present investigation were (i) to isolate oak ellagitannins and to hemisynthesize some made-in-wine flavano-ellagitannins, such as acutissimin A; (ii) to analyse their concentration ranges depending on the cultivar area and (iii) to evaluate their sensory impact on the basis of their human threshold concentrations and dose/response relationships in different types of solutions.

Comparison of fortified, sfursat and passito winemaking techniques for the enhancement of the oenological potential of the black grape cultivar Moscato nero d’Acqui (Vitis vinifera L.)

One of the key factors of the economical development of viticulture and wine industry in specific limited areas is the exploitation of ancient, local grape varieties. Therefore, in recent years the growing interest to rediscover minor varieties, previously cultivated, has promoted many studies. With this regard, the focus of this study was the Vitis vinifera L. cultivar Moscato nero d’Acqui, nowadays found only in old vineyards in the Acqui zone (North-West Italy). In particular, the aims of this work were: i) to investigate secondary metabolites profile of the grapes, and ii) to evaluate the attitude to the production of special wines.

A preliminary study of clonal selection in cv. Viura in relation to varietal aroma profile

Viura is a synonym for Macabeo and currently it is the most widely planted white grape variety in D.O.Ca. Rioja, with 3,569 ha, representing 84% of the white grape cultivated area. It is a generous-yielding grape, presenting low values of titratable acidity and with large and compact clusters which makes it susceptible to Botrytis cinerea. Thus, this variety not always satisfies the wine grower’s prospects. Nowadays, the available plant material is scarce, moreover, it was selected on the basis of other quality criteria, not currently requested.