Macrowine 2021
IVES 9 IVES Conference Series 9 New biological tools to control and secure malolactic fermentation in high pH wines

New biological tools to control and secure malolactic fermentation in high pH wines

Abstract

Originally, the role of the malolactic fermentation (MLF) was simply to improve the microbial stability of wine via biological deacidification. However, there is an accumulation of evidence to support the fact that lactic acid bacteria (LAB) also contribute positively to the taste and aroma of wine. Many different LAB enter into grape juice and wine from the surface of grape berries, cluster stems, vine leaves, soil and winery equipment. Due to the highly selective environment of juices and wine, only a few types of LAB are able to grow. Wine pH is most selective, and at pH below 3.5 generally only strains of Oenococcus oeni can survive and express malolactic activity, while wines with pH above 3.5 can contain various species of Pediococcus, as well as strains of Lactobacillus. The trend toward harvesting higher maturity grapes has resulted in the processing of higher pH musts and the production of wines containing increased levels of alcohol. These conditions favor the growth of indigenous bacteria and often O. oeni does not prevail at the end of alcoholic fermentation. More Lactobacillus sp. predominate and are often responsible for spontaneous MLF (du Toit et al. 2011). Some L. plantarum strains can tolerate the high alcohol concentrations and SO2 levels normally encountered in wine. Due to their very complex and diverse metabolism a range of compositional changes can be induced, which may affect the quality of the final product positively or negatively. A recent isolate have shown most interesting results, not only for its capacity to induce MLF after direct inoculation in freeze-dried form, but also for their positive contribution to the wine aroma. Co-inoculation (inoculation of selected wine LAB 24 hours after the yeast) can ensure the early implantation and dominance of the selected strain, the early onset and completion of MLF, and can possibly prevent the appearance of the spoilage yeast and bacteria. Applying an important L. plantarum inoculum with high malolactic activity assures an immediate dominance, as well as predictable and complete MLF in short time and allows an early stabilization of the wine. Since it degrades hexose sugars by the homo-fermentative pathway, which poses no risk of acetic acid production from the residual sugars that may be present in high pH wines, it is an interesting alternative to control MLF in high pH wines.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Sibylle Dr. Krieger-Weber*, Anthony Silvano, Magali Deleris-Bou

*Lallemand SAS

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Cover crops influence on soil N availability and grapevine N status, and its relationship with biogenic

The type of soil management, tillage versus cover crops, can modify the soil microbial activity, which causes the mineralization of organic N to NO3–N and, therefore, may change the soil NO3–N availability in vineyard. The soil NO3–N availability could influence the grapevine nutritional status and the grape amino acid composition. Amino acids are precursors of biogenic amines, compounds mainly formed during the malolactic fermentation. Biogenic amines have negative effects on consumer health and on the wine organoleptic quality. The objective was to study if the effect of conventional tillage and two different cover crops (leguminous versus gramineous) on grapevine N status, could relate to the wine biogenic amines composition.

Impact of glutathione and elemental sulphur juice addition on the volatile thiol production in South African Sauvignon blanc wine

Three compounds, 3-mercaptohexanol (3MH), 3-mercaptohexyl-acetate (3MHA) and 4-mercapto-4-methylpentan-2-one (4MMP), also known as varietal thiols, have been identified to contribute positively to wine aroma and are responsible for the distinct gooseberry, grapefruit, guava and box tree character found in Sauvignon blanc wines. Certain volatile thiol compounds though, can cause off-aromas of onion, garlic, rubber and rotten egg, this group of molecules is known as reductive sulphur compounds (RSC). This study looks into how the addition of sulphur-compounds to Sauvignon blanc juice contributes to the varietal thiol (3MH and 3MHA) concentration and reductive sulphur compound concentration in South African Sauvignon blanc wine.

Evaluation of colloidal stability in white and rosé wines investing Dynamic Light Scattering technology

Proteins constitute one of the three main components of grape juice and white wine, phenolic compounds and polysaccharides being the others. A specific group of the total grape-derived proteins resists degradation or adsorption during the winemaking process and remains in finished wine if not removed by the commonplace commercial practice of bentonite fining. While bentonite is effective in removing the problematic proteins, it is claimed to adversely affect the quality of the treated wine under certain conditions, through the removal of colour, flavor and texture compounds. A number of studies have indicated that different protein fractions require distinct bentonite concentrations for protein removal and consequent heat stabilization.

A preliminary study of clonal selection in cv. Viura in relation to varietal aroma profile

Viura is a synonym for Macabeo and currently it is the most widely planted white grape variety in D.O.Ca. Rioja, with 3,569 ha, representing 84% of the white grape cultivated area. It is a generous-yielding grape, presenting low values of titratable acidity and with large and compact clusters which makes it susceptible to Botrytis cinerea. Thus, this variety not always satisfies the wine grower’s prospects. Nowadays, the available plant material is scarce, moreover, it was selected on the basis of other quality criteria, not currently requested.

Effect of malolactic fermentation in barrels or stainless steel tanks on wine composition. Influence of the barrel toasting

Ellagitannin, anthocyanin and woody volatile composition of Cabernet Sauvignon wines aged in oak barrels for 12 months was evaluated. Depending on the container where malolactic fermentation (MLF) was carried out, two wine modalities were investigated: wines with MLF carried out in stainless steel tanks and barrel-fermented wines. Three toasting methods (medium toast, MT; medium toast with watering, MTAA; noisette) were considered for ageing of each wine modality. Sensory analyses (triangle and rating tests) were also performed. Two-way ANOVA of the raw experimental data revealed that the toasting method and the container where MLF took place, as well as the interaction between both factors, have a significant influence (p < 0.05) on ellagitannin, anthocyanin and woody volatile profiles of Cabernet Sauvignon wines.