Macrowine 2021
IVES 9 IVES Conference Series 9 New biological tools to control and secure malolactic fermentation in high pH wines

New biological tools to control and secure malolactic fermentation in high pH wines

Abstract

Originally, the role of the malolactic fermentation (MLF) was simply to improve the microbial stability of wine via biological deacidification. However, there is an accumulation of evidence to support the fact that lactic acid bacteria (LAB) also contribute positively to the taste and aroma of wine. Many different LAB enter into grape juice and wine from the surface of grape berries, cluster stems, vine leaves, soil and winery equipment. Due to the highly selective environment of juices and wine, only a few types of LAB are able to grow. Wine pH is most selective, and at pH below 3.5 generally only strains of Oenococcus oeni can survive and express malolactic activity, while wines with pH above 3.5 can contain various species of Pediococcus, as well as strains of Lactobacillus. The trend toward harvesting higher maturity grapes has resulted in the processing of higher pH musts and the production of wines containing increased levels of alcohol. These conditions favor the growth of indigenous bacteria and often O. oeni does not prevail at the end of alcoholic fermentation. More Lactobacillus sp. predominate and are often responsible for spontaneous MLF (du Toit et al. 2011). Some L. plantarum strains can tolerate the high alcohol concentrations and SO2 levels normally encountered in wine. Due to their very complex and diverse metabolism a range of compositional changes can be induced, which may affect the quality of the final product positively or negatively. A recent isolate have shown most interesting results, not only for its capacity to induce MLF after direct inoculation in freeze-dried form, but also for their positive contribution to the wine aroma. Co-inoculation (inoculation of selected wine LAB 24 hours after the yeast) can ensure the early implantation and dominance of the selected strain, the early onset and completion of MLF, and can possibly prevent the appearance of the spoilage yeast and bacteria. Applying an important L. plantarum inoculum with high malolactic activity assures an immediate dominance, as well as predictable and complete MLF in short time and allows an early stabilization of the wine. Since it degrades hexose sugars by the homo-fermentative pathway, which poses no risk of acetic acid production from the residual sugars that may be present in high pH wines, it is an interesting alternative to control MLF in high pH wines.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Sibylle Dr. Krieger-Weber*, Anthony Silvano, Magali Deleris-Bou

*Lallemand SAS

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Merging fast sensory profiling with non-targeted GC-MS analysis for multifactorial experimental wine making

Wine aroma is influenced by several viticultural and oenological factors. In this study we used experimental wine making in a full factorial design to determine the impact of grapevine age, must turbidity, and yeast strain on the aroma of Vitis vinifera L. cv. Riesling wines. A recently developed, non-targeted SPME-GC-MS fingerprinting approach for wine volatiles was used. This approach includes the segmentation and mathematical transformation of chromatograms in combination with Parallel Factor Analysis (PARAFAC) and subsequent deconvolution of important chromatogram segments.

IBMP-Polypenol interactions: Impact on volatility and sensory perception in model wine solution

3-Isobutyl-2-methoxypyrazine (IBMP) is one of the key molecules in wine aroma with a bell pepper aroma and a very low threshold in wine, 1-6 ng/L for white wine and 10-16 ng/L in red wine1. The differences in these thresholds are likely due to IBMP-non volatile matrix interactions. It has indeed been shown that polyphenols may influence the volatility of flavor compounds2. In the present study, we focus on IBMP-polyphenols interactions in relation to volatility and sensory perception in model wine solution. Methods: 1. GC-MS Static Headspace Analysis: Samples were analyzed by Static headspace analysis with an Agilent 7890A gas chromatograph coupled to HP 5975C mass spectrometry detector (Agilent Technologies, Santa Clara, CA, USA).

Influence of wood chips addition during alcoholic fermentation on wine phenolic composition

This study investigates the effect of wood chips addition during the alcoholic fermentation on the phenolic
composition of the produced wines. A series of wood chips, originating from American, French, Slavonia
oak and Acacia were added at the beginning of wine alcoholic fermentation. Besides, a mixture consisting
of 50% French and 50% Americal oak chips were added during the experimentation. The wine samples
were analyzed one month after the end of malolactic fermentation, examining various chemical
parameters such as total anthocyanins, total phenolic content, tannins combined with protein (BSA) and
ellagitannin content.

Assessing the effect of oak derived aromas on mouthfeel perception in Chardonnay wine

Mouthfeel is an important quality parameter for Chardonnay wines, particularly those aged in oak. While research on mouthfeel has traditionally focused on the impact of non-aromatic compounds, the role of aroma compounds has largely been over looked. However, in wine as well as other food interactions between retronasal aroma and mouthfeel have been noted. The goal of this research was to investigate the impact of wine aroma on the perception of mouthfeel. Because of the importance of oak aging in the development of Chardonnay mouthfeel, the impact of oak aromas on perceived mouthfeel was explored. Aroma compounds associated with oak (ethyl palmitate, eugenol, furfural, isoeugenol, syringaldehyde, vanillin and whiskey lactone) were added to two different Chardonnay wines; one with no oak influence and one fermented in neutral oak. Low and high concentrations of the compounds were added based on concentrations typically found in barrel aged Chardonnay wine.

An excessive leaf-fruit ratio reduces the yeast assimilable nitrogen in the must

Yeast assimilable nitrogen (YAN) in the grape must is a key variable for wine quality as a source of aroma precursors. In a situation of YAN deficiency, a foliar urea application upon the vine at veraison enhances YAN concentration and facilitates must fermentation. In 2013, Agroscope investigated the impact of leaf-fruit ratio on the nitrogen (N) assimilation and partitioning in grapevine Vitis vinifera cv. Chasselas following foliar-urea application with the aim of improving its efficiency on the YAN concentration.