Macrowine 2021
IVES 9 IVES Conference Series 9 Phenolic profiles of minor red grape cultivars autochthonous from the Spanish region of La Mancha

Phenolic profiles of minor red grape cultivars autochthonous from the Spanish region of La Mancha

Abstract

The phenolic profiles of little known red grape cultivars, namely Garnacho, Moribel and Tinto Fragoso, which are autochthonous from the Spanish region of La Mancha (ca. 600,000 ha of vineyards) have been studied over the consecutive seasons of years 2013 and 2014. The study was separately performed over the skins, the pulp and the seeds, and comprised the following phenolic types: anthocyanins, flavonols, hydroxycinnamic acid derivatives (HCADs), total proanthocyanidins (PAs) and their structural features. The selected grape cultivars belong to the Vine Germplasm Bank created in this region in order to preserve the great diversity of genotypes grown in La Mancha. This study aims to evaluate the enological potential of these minor grape cultivars. Regarding anthocyanins, the three studied cultivars showed the occurrence of the 3-glucosides of the common grape anthocyanidins, with the prevalence of malvidin-based anthocyanins, followed by peonidin derivatives. However, Garnacho showed a characteristic profile in which anthocyanins based on peonidin and malvidin accounted for similar proportions. Among the acylated anthocyanins, the coumaroyl derivatives dominated over the acetylated ones and some minor caffeoyl derivatives were also found. In addition, minor anthocyanidin 3,5-diglucosides were detected, mainly malvidin 3,5-diglucoside, although its coumaroyl derivative was also found in Tinto Fragoso and peonidin 3,5-diglucoside in the case of Garnacho. Tinto Fragoso showed the highest content of anthocyanins in both years. Flavonol profiles were in agreement with those previously described for V. vinifera grapes: the 3-glucosides, the 3-galactosides and the 3-glucuronides of the six common aglycones, namely kaempferol, quercetin, isorhamnetin, myricetin, laricitrin and syringetin. Moreover, high resolution MS and MS/MS evidence of the occurrence of dihexosides of myricetin was found in grapes. The qualitative content of HCADs showed no differences according to grape cultivar and was dominated by caftaric acid. The pulp accumulated most HCADs. The content of PAs in grape skins does not vary according to grape variety and season year, around 8-13 mg/g skin (as catechin). In contrast, the content of PAs in seeds was lower in Moribel (53-58 mg/g, vs. 75-79 mg/g in Garnacho and 81-86 mg/g in Tinto Fragoso), but no differences were found according to season year. The mean degree of polymerization was similar in the three cultivars and two season years: 9-10 for skin PAs and 6-7 for seed PAs. The percentage of prodelphinidins in skin PAs ranged within 17-18% for the three cultivars, whereas the percentage of galloylation in seed PAs was lower in Moribel (13%, vs. 16-17% in the other two cultivars). Finally, the season year of 2014 was characterized by adverse climatic conditions with regards to the development of grapes and the contents of anthocyanins and flavonols were affected showing lower concentrations for the three grape cultivars.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Isidro Hermosín-Gutíerrez*, Esteban García-Romero, Jesús Martínez-Gascueña, José Luís Chacón-Vozmedian, José Pérez-Navarro, Pedro Izquierdo-Cañas, Sergio Gómez-Alonso

*Universidad de Castilla-La Mancha

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

How small amounts of oxygen introduced during bottling and storage can influence the metabolic fingerprint and SO2 content of white wines

The impact of minute amounts of headspace oxygen on the post-bottling development of wine is generally considered to be very important, since oxygen, packaging and storage conditions can either damage or improve wine quality. This is reflected in the generalised use of inert bottling lines, where the headspace between the white wine and the stopper is filled with an inert gas. This experiment aimed to address some open questions about the chemistry of the interaction between wine and oxygen, crucial for decisions regarding optimal closure. While it is known that similar amounts of oxygen affect different wines to a variable extent, our knowledge of chemistry is not sufficient to construct a predictive method.

An excessive leaf-fruit ratio reduces the yeast assimilable nitrogen in the must

Yeast assimilable nitrogen (YAN) in the grape must is a key variable for wine quality as a source of aroma precursors. In a situation of YAN deficiency, a foliar urea application upon the vine at veraison enhances YAN concentration and facilitates must fermentation. In 2013, Agroscope investigated the impact of leaf-fruit ratio on the nitrogen (N) assimilation and partitioning in grapevine Vitis vinifera cv. Chasselas following foliar-urea application with the aim of improving its efficiency on the YAN concentration.

Sensory definition of green aroma concept in red French wines. Evidence for the contribution of novel volatile markers

The aromatic complexity of a wine results from the perception of the association of volatile molecules and each aroma can be categorized into different families. The “green” aromas family in red wines has retained our attention by its close link with the fruity perception. In that study, the “green” olfactory concept of red wines was considered through a strategy combining both sensory analysis and hyphenated chromatographic techniques including HPLC and MDGC (Multidimensional Gas Chromatography). The aromatic space of this concept was specified by lexical generation through a free association task on 22 selected wines by a panel of wine experts. Then, 70 French red wines were scored on the basis of the intensity of their “green” and “fruity” attributes.

Fingerprinting the origin of rosé wines with a new high throughput polyphenomics method

Wine is a widely consumed alcoholic beverage with a high commercial value. More specifically, the worldwide consumption of rosé wine has increased by 20% since 2002[1]. But because of its high commercial value, it can become a subject of fraud, and authenticity control is necessarily required. More than one hundred polyphenols have been recently quantified in various rosé wines [2]. They are key components defining color, taste and quality of wines. Their amount and composition depend on many different factors such as grape variety, winemaking and age of the wine. In this study, the influence of geographic origin of some rosé French wines was investigated. An original and very fast UPLC-QTOF-MS method was developed and used to predict the geographic origin authenticity of rosé wines.

A multivariate approach using attenuated total reflectance mid-infrared spectroscopy to measure the surface mannoproteins and β-glucans of yeast cell walls during wine fermentations

Yeast cells possess a cell wall comprising primarily glycoproteins, mannans, and glucan polymers. Several yeast phenotypes relevant for fermentation, wine processing, and wine quality are correlated with cell wall properties. To investigate the effect of wine fermentation on cell wall composition, a study was performed using mid-infrared (MIR) spectroscopy coupled with multivariate methods (i.e., PCA and OPLS-DA). A total of 40 yeast strains were evaluated, including Saccharomyces strains (laboratory and industrial) and non-Saccharomyces species. Cells were fermented in both synthetic MS300 and Chardonnay grape must to stationery phase, processed, and scanned in the MIR spectrum.