Macrowine 2021
IVES 9 IVES Conference Series 9 Phenolic profiles of minor red grape cultivars autochthonous from the Spanish region of La Mancha

Phenolic profiles of minor red grape cultivars autochthonous from the Spanish region of La Mancha

Abstract

The phenolic profiles of little known red grape cultivars, namely Garnacho, Moribel and Tinto Fragoso, which are autochthonous from the Spanish region of La Mancha (ca. 600,000 ha of vineyards) have been studied over the consecutive seasons of years 2013 and 2014. The study was separately performed over the skins, the pulp and the seeds, and comprised the following phenolic types: anthocyanins, flavonols, hydroxycinnamic acid derivatives (HCADs), total proanthocyanidins (PAs) and their structural features. The selected grape cultivars belong to the Vine Germplasm Bank created in this region in order to preserve the great diversity of genotypes grown in La Mancha. This study aims to evaluate the enological potential of these minor grape cultivars. Regarding anthocyanins, the three studied cultivars showed the occurrence of the 3-glucosides of the common grape anthocyanidins, with the prevalence of malvidin-based anthocyanins, followed by peonidin derivatives. However, Garnacho showed a characteristic profile in which anthocyanins based on peonidin and malvidin accounted for similar proportions. Among the acylated anthocyanins, the coumaroyl derivatives dominated over the acetylated ones and some minor caffeoyl derivatives were also found. In addition, minor anthocyanidin 3,5-diglucosides were detected, mainly malvidin 3,5-diglucoside, although its coumaroyl derivative was also found in Tinto Fragoso and peonidin 3,5-diglucoside in the case of Garnacho. Tinto Fragoso showed the highest content of anthocyanins in both years. Flavonol profiles were in agreement with those previously described for V. vinifera grapes: the 3-glucosides, the 3-galactosides and the 3-glucuronides of the six common aglycones, namely kaempferol, quercetin, isorhamnetin, myricetin, laricitrin and syringetin. Moreover, high resolution MS and MS/MS evidence of the occurrence of dihexosides of myricetin was found in grapes. The qualitative content of HCADs showed no differences according to grape cultivar and was dominated by caftaric acid. The pulp accumulated most HCADs. The content of PAs in grape skins does not vary according to grape variety and season year, around 8-13 mg/g skin (as catechin). In contrast, the content of PAs in seeds was lower in Moribel (53-58 mg/g, vs. 75-79 mg/g in Garnacho and 81-86 mg/g in Tinto Fragoso), but no differences were found according to season year. The mean degree of polymerization was similar in the three cultivars and two season years: 9-10 for skin PAs and 6-7 for seed PAs. The percentage of prodelphinidins in skin PAs ranged within 17-18% for the three cultivars, whereas the percentage of galloylation in seed PAs was lower in Moribel (13%, vs. 16-17% in the other two cultivars). Finally, the season year of 2014 was characterized by adverse climatic conditions with regards to the development of grapes and the contents of anthocyanins and flavonols were affected showing lower concentrations for the three grape cultivars.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Isidro Hermosín-Gutíerrez*, Esteban García-Romero, Jesús Martínez-Gascueña, José Luís Chacón-Vozmedian, José Pérez-Navarro, Pedro Izquierdo-Cañas, Sergio Gómez-Alonso

*Universidad de Castilla-La Mancha

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Improving the phenolic composition of cv tempranillo wines by blending grapes of different ripening state

The aim of this work was to reduce the alcohol content of Tempranillo wine. Tempranillo wines were produced by grapes harvested at different ripening dates (August 11 which was 21 oBrix and September 28 with 25 oBrix). At the second date, the Tempranillo wines were elaborated as follows: grapes were destemmed, crushed and collected into 50 L stainless-steel vats. Before preferementative maceration in cold, 50 % (M1) and 70 % (M2) of the must have been replaced by the same percentage of must from the first harvest. In addition, a control wine (C) was performed with only grapes from the second harvest.

Grape metabolites, aroma precursors and the complexities of wine flavour

A critical aspect of wine quality from a consumer perspective is the overall impression of wine flavour, which is formed by the interplay of volatile aroma compounds, their precursors, and taste and matrix components. Grapes contribute some potent aroma compounds, together with a large pool of non-volatile precursors (e.g. glycoconjugates and amino acid conjugates). Aroma precursors can break down through chemical hydrolysis reactions, or through the action of yeast or enzymes, significantly changing the aroma profile of a wine during winemaking and storage. In addition, glycoconjugates of monoterpenes, norisoprenoids and volatile phenols, together with sulfur-conjugates in wine, provide a reservoir of additional flavour through the in-mouth release of volatiles which may be perceived retro-nasally.

Impact of smoke exposure on the chemical composition of grapes

Vineyard exposure to smoke can lead to grapes and wine which exhibit objectionable smoky and ashy aromas and flavours, more commonly known as ‘smoke taint’ [1, 2]. In the last decade, significant bushfires have occurred around the world, including near wine regions in Australia, Canada, South Africa and the USA, as a consequence of the warmer, drier conditions associated with climate change. Considerable research has subsequently been undertaken to determine the chemical, sensory and physiological consequences of grapevine exposure to smoke. The sensory attributes associated with smoke-tainted wine have been linked to the presence of several smoke-derived volatile phenols, such as guaiacols, syringols and cresols [2].

Impact of drought stress on concentration and composition of wine proteins in Riesling

Protein haze in white wines is a major technological and economic problem of the wine industry. Field tests were carried out in steep slope vineyards planted with Riesling grapes over 3 dry growing seasons to study the effect of drought stress on the concentration of proteins in the resulting wines. Plots suffering from drought stress were compared with surrounding drip irrigated plots. Riesling grapes were processed into wines by conventional procedures. Protein amounts of the isolated wine colloids of the stressed samples were always higher than those of the watered samples(mean watered 13.8 ± 0.44, mean stressed 17.4 ± 0.40 g 100 g-1). As a consequence, higher bentonite doses were needed to achieve protein haze stability of the drought stressed treatments.

Foam characteristics of white, rosé and red sparkling wines elaborated by the champenoise method

Contribution Foam is the characteristic that differentiates sparkling wines from still wines, being the first sensory attribute that tasters and consumers perceive and that determines the final quality of sparkling wines [1]. The foaming properties mainly depend on the chemical composition of wines [2-3], and different factors involved in wine composition will have an effect on foam quality. In Spain, the sparkling wine market focuses on the production of white and rosé sparkling wine, with very low production of red sparkling wines. However, this type of wines is elaborated in countries like Australia, South-Africa, Argentina, Italy or Portugal, with a great acceptance by consumers. No studies on the foaming characteristics of red sparkling wines have been found.