Macrowine 2021
IVES 9 IVES Conference Series 9 Phenolic profiles of minor red grape cultivars autochthonous from the Spanish region of La Mancha

Phenolic profiles of minor red grape cultivars autochthonous from the Spanish region of La Mancha

Abstract

The phenolic profiles of little known red grape cultivars, namely Garnacho, Moribel and Tinto Fragoso, which are autochthonous from the Spanish region of La Mancha (ca. 600,000 ha of vineyards) have been studied over the consecutive seasons of years 2013 and 2014. The study was separately performed over the skins, the pulp and the seeds, and comprised the following phenolic types: anthocyanins, flavonols, hydroxycinnamic acid derivatives (HCADs), total proanthocyanidins (PAs) and their structural features. The selected grape cultivars belong to the Vine Germplasm Bank created in this region in order to preserve the great diversity of genotypes grown in La Mancha. This study aims to evaluate the enological potential of these minor grape cultivars. Regarding anthocyanins, the three studied cultivars showed the occurrence of the 3-glucosides of the common grape anthocyanidins, with the prevalence of malvidin-based anthocyanins, followed by peonidin derivatives. However, Garnacho showed a characteristic profile in which anthocyanins based on peonidin and malvidin accounted for similar proportions. Among the acylated anthocyanins, the coumaroyl derivatives dominated over the acetylated ones and some minor caffeoyl derivatives were also found. In addition, minor anthocyanidin 3,5-diglucosides were detected, mainly malvidin 3,5-diglucoside, although its coumaroyl derivative was also found in Tinto Fragoso and peonidin 3,5-diglucoside in the case of Garnacho. Tinto Fragoso showed the highest content of anthocyanins in both years. Flavonol profiles were in agreement with those previously described for V. vinifera grapes: the 3-glucosides, the 3-galactosides and the 3-glucuronides of the six common aglycones, namely kaempferol, quercetin, isorhamnetin, myricetin, laricitrin and syringetin. Moreover, high resolution MS and MS/MS evidence of the occurrence of dihexosides of myricetin was found in grapes. The qualitative content of HCADs showed no differences according to grape cultivar and was dominated by caftaric acid. The pulp accumulated most HCADs. The content of PAs in grape skins does not vary according to grape variety and season year, around 8-13 mg/g skin (as catechin). In contrast, the content of PAs in seeds was lower in Moribel (53-58 mg/g, vs. 75-79 mg/g in Garnacho and 81-86 mg/g in Tinto Fragoso), but no differences were found according to season year. The mean degree of polymerization was similar in the three cultivars and two season years: 9-10 for skin PAs and 6-7 for seed PAs. The percentage of prodelphinidins in skin PAs ranged within 17-18% for the three cultivars, whereas the percentage of galloylation in seed PAs was lower in Moribel (13%, vs. 16-17% in the other two cultivars). Finally, the season year of 2014 was characterized by adverse climatic conditions with regards to the development of grapes and the contents of anthocyanins and flavonols were affected showing lower concentrations for the three grape cultivars.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Isidro Hermosín-Gutíerrez*, Esteban García-Romero, Jesús Martínez-Gascueña, José Luís Chacón-Vozmedian, José Pérez-Navarro, Pedro Izquierdo-Cañas, Sergio Gómez-Alonso

*Universidad de Castilla-La Mancha

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Metabolomic profile of red non-V. vinifera genotypes

Vitis vinifera L. is the most widely cultivated Vitis species which includes numerous cultivars. Owing to their superior quality of grapes, these cultivars were long considered the only suitable for the production of fine wines. However, the lack of resistance genes in V. vinifera against major grapevine pathogens, requires for its cultivation frequent spraying with large amount of fungicides. Thus, the search for alternative and more sustainable methods to control the grapevine pathogens have brought the breeders to focus their attention on other Vitis species. In fact, wild Vitis genotypes present multiple resistance traits against pathogens, such as powdery mildew, downy mildew and phylloxera.

Analysis of the oenological potentials of different oak forests in Hungary

Like France, Hungary has many oak forests used for making barrels since many years. But if the differences between the woods of the North, the East and the South-West forests of France are well known, this is probably not the case of Hungarian forests. However taking into account the essential differences of climates and soils, differences must be significant and the general name “Hungarian oak” must not have any real meaning. We have studied precisely (determination of concentrations of volatile and non-volatile wood compounds, anatomical criteria, measurement of antioxidant capacity) of oaks collected from northeastern Hungary and others collected from the Danube valley in the northwest of the country.

Impact of sulfur compounds to the antioxidant stability of white wines

The chemical mechanisms involved in oxidation/reduction potential of wine during winemaking and aging are affecting its color, aroma and taste. Chemical oxidation is one of the major causes of development of off-flavors during ageing1. Thus, the chemical changes in wine during storage should be controlled to ensure the sensory quality of the product and avoid consumer rejection that will compromise the economic value of the product. The 1-hydroxyethyl radical has been recognized as the key radical intermediate in the oxidative reactions in wine2. Based on the kinetic study of POBN-1-hydroxyethyl spin adduct formation in wines initiated via the Fenton reaction, a novel tool was recently developed in our laboratory to quantify the resistance of wines against oxidation3.

Prevention of wine oxidation during barrel aging: an innovative method to measure antioxidant

Wine oxidation is a problem that affects the freshness, the aromatic profile, the colour and also the mouthfeel of the wine. It mainly concerns white wines. Oxygen interactions with wine compounds lead to the phenomena cited above that are responsible for the depreciation of these wines. Barrel aging is a crucial step in the wine process because it allows many modifications as wine enrichment, colour stabilization, clarification and also a slow oxygenation of the wine. Effects of the oak barrel have to be known to prevent oxidation of the wine. We have been interested in the main antioxidant compounds released by oak barrels to the wine and we have developed an innovative method to reach directly these antioxidant compounds at the oak stave surface.

The role of tomato juice serum in malolactic fermentation in wine

Introduction: Malolactic fermentation (MLF) is a common process in winemaking to reduce wine acidity, maintain microbial stability and modify wine aroma. However, successful MLF is often hampered by their sluggish or stuck activity of malolactic bacteria (MLB) which may be caused by nutrient deficiency, especially when MLB are inoculated after alcoholic fermentation (Alexandre et al., 2004; Lerm et al., 2010). Identification and characterization of essential nutrients and growth factors for MLB allows for production of highly efficient nutrient supplements for MLF.