Macrowine 2021
IVES 9 IVES Conference Series 9 Phenolic profiles of minor red grape cultivars autochthonous from the Spanish region of La Mancha

Phenolic profiles of minor red grape cultivars autochthonous from the Spanish region of La Mancha

Abstract

The phenolic profiles of little known red grape cultivars, namely Garnacho, Moribel and Tinto Fragoso, which are autochthonous from the Spanish region of La Mancha (ca. 600,000 ha of vineyards) have been studied over the consecutive seasons of years 2013 and 2014. The study was separately performed over the skins, the pulp and the seeds, and comprised the following phenolic types: anthocyanins, flavonols, hydroxycinnamic acid derivatives (HCADs), total proanthocyanidins (PAs) and their structural features. The selected grape cultivars belong to the Vine Germplasm Bank created in this region in order to preserve the great diversity of genotypes grown in La Mancha. This study aims to evaluate the enological potential of these minor grape cultivars. Regarding anthocyanins, the three studied cultivars showed the occurrence of the 3-glucosides of the common grape anthocyanidins, with the prevalence of malvidin-based anthocyanins, followed by peonidin derivatives. However, Garnacho showed a characteristic profile in which anthocyanins based on peonidin and malvidin accounted for similar proportions. Among the acylated anthocyanins, the coumaroyl derivatives dominated over the acetylated ones and some minor caffeoyl derivatives were also found. In addition, minor anthocyanidin 3,5-diglucosides were detected, mainly malvidin 3,5-diglucoside, although its coumaroyl derivative was also found in Tinto Fragoso and peonidin 3,5-diglucoside in the case of Garnacho. Tinto Fragoso showed the highest content of anthocyanins in both years. Flavonol profiles were in agreement with those previously described for V. vinifera grapes: the 3-glucosides, the 3-galactosides and the 3-glucuronides of the six common aglycones, namely kaempferol, quercetin, isorhamnetin, myricetin, laricitrin and syringetin. Moreover, high resolution MS and MS/MS evidence of the occurrence of dihexosides of myricetin was found in grapes. The qualitative content of HCADs showed no differences according to grape cultivar and was dominated by caftaric acid. The pulp accumulated most HCADs. The content of PAs in grape skins does not vary according to grape variety and season year, around 8-13 mg/g skin (as catechin). In contrast, the content of PAs in seeds was lower in Moribel (53-58 mg/g, vs. 75-79 mg/g in Garnacho and 81-86 mg/g in Tinto Fragoso), but no differences were found according to season year. The mean degree of polymerization was similar in the three cultivars and two season years: 9-10 for skin PAs and 6-7 for seed PAs. The percentage of prodelphinidins in skin PAs ranged within 17-18% for the three cultivars, whereas the percentage of galloylation in seed PAs was lower in Moribel (13%, vs. 16-17% in the other two cultivars). Finally, the season year of 2014 was characterized by adverse climatic conditions with regards to the development of grapes and the contents of anthocyanins and flavonols were affected showing lower concentrations for the three grape cultivars.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Isidro Hermosín-Gutíerrez*, Esteban García-Romero, Jesús Martínez-Gascueña, José Luís Chacón-Vozmedian, José Pérez-Navarro, Pedro Izquierdo-Cañas, Sergio Gómez-Alonso

*Universidad de Castilla-La Mancha

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Proteomic and activity characterization of exocellular laccases from three Botrytis cinerea strains

Botrytis cinerea is a fungus that causes common infection in grapes and other fruits. In winemaking, its presence can be both considered desirable in the case of noble rot infection or undesirable when grey rot is developed. This fungus produces an extracellular enzyme known as laccase which is able to cause oxidation of phenolic compounds present in must and wine, causing most of the times a decrease in its quality and problems during the winemaking process [1]. Material and methods: Three B. cinerea strains (B0510, VA612 and RM344) were selected and grown in a liquid medium adapted from one previously described [2]. The enzyme was isolated by tangential ultrafiltration of the culture medium using a QuixStand system equipped with a 30 KDa filtration membrane.

South Africa’s top 10 Sauvignon blanc wines. How do the chemical and sensory profiles compare?

FNB Top 10 Sauvignon Blanc competition, presented by the Sauvignon Blanc Interest Group of South Africa and sponsored by First National Bank, is the country’s foremost platform for producers of this cultivar to showcase and benchmark their wines. Wines entered in the competition originated from all over the winegrowing regions of the country and the winning wines showed good representation of quality South African Sauvignon blanc wines. The ten selected wines were subjected to various chemical analyses including volatile thiol and methoxypyrazine determination, while the sensory profile of each wine was determined using projective mapping.

Characterization of various groups of pyranoanthocyanins in Merlot red wine

In red wines, anthocyanins evolve during the wine-making process and ageing. They react with other compounds (such as vinylphenols, acetaldehyde, pyruvic acid…) to form a stable family of compounds called pyranoanthocyanins. Furthermore, the oxidation process can modify the anthocyanic profile of a red wine. It is also interesting to evaluate the occurrence of the different subclasses of pyranoanthocyanins and to characterize their chemical properties. The first objective of this study is to evaluate the occurrence of the different groups of pyranoanthocyanins in an oxidised Merlot wine by a centrifugal partition chromatography strategy. The second goal is to evaluate their relative impact in red wines from Bordeaux region by measuring their concentrations.

Correlations between N,S,O-heterocycle levels and age of Champagne base wines

Champagne regulation allows winegrowers to stock small amounts of still wines in order to compensate vintages’ quality shifts mainly due to climate variations. According to their technical requirements and house style some Champagne producers (commonly named “Champagne houses”) use these stored wines in the blend in order to introduce an element of complexity. These wines possess the particularity of being aged on fine lees in thermo-regulated stainless steel tanks. The Champagne house of Veuve Clicquot Ponsardin has several wines stored this way.

Sensory impacts of the obturator used for the Chasselas: study over the time

Many parameters affect the organoleptic characteristics of wine: internal parameters like the chemical composition or polyphenol content and external as for example storage conditions or the type of obturator. The aim of this study was to characterize sensorally the impacts of several type of obturator on a white wine: Chasselas. To determine the organoleptic characteristics of this wine, a quantitative descriptive analysis could be used. But rapid sensory methods were preferred in this project. Indeed these methods are an appropriate alternative to conventional descriptive methods for quickly assessing sensory product discrimination.