Macrowine 2021
IVES 9 IVES Conference Series 9 Anthocyanin accumulation and extractability during the maturation of the grapes of three varieties

Anthocyanin accumulation and extractability during the maturation of the grapes of three varieties

Abstract

Anthocyanin accumulation and extractability were studied in Tannat, Cabernet Sauvignon and Merlot grapes produced in the south of Uruguay in two consecutive seasons. Typical cultivation situations employed in the region for each variety were considered. A follow-up was carried out, considering 60 plants per vineyard, and the harvest was determined according to the technological indices of maturity. Samples of grapes were taken in duplicate in each vineyard periodically along grape maturation. The basic composition, polyphenolic potential and anthocyanin extractability were determined. Also, half of grapes were frozen and later peeled; skin extractions over 24 hs with a solution of 12% ethanol and pH 3.2 were carried out. The anthocyanin contents of the extracts obtained were determined by HPLC-DAD. The levels of anthocyanins reached the highest values before technological maturity. Anthocyanin extractability had a decrease during grape maturation. The highest levels of anthocyanins were found in Tannat skins altough those of Cabernet Sauvignon were higher than those of Merlot grapes. Anthocyanin profiles of skins changing significantly after veraison but these changes were very lower just before maturity, found the typical values of each variety The prevalent anthocyanin derivatives at the start of the maturation showed a decrease during the process. Malvidin proportions increased and cyanidin proportions decreased along the first stages of maturation, in agree with the biosynthesis of anthocyanins. Malvidin derivatives showed the fastest accumulation. At harvest, Tannat berries presented the highest sugar contents, total acidity, total polyphenol richness, total potential in anthocyanins and potential in extractable anthocyanins, and the lowest pH values. Tannat had the highest values of EA%, corresponding to the lowest extractability of anthocyanins. Significant differences among the anthocyanin profiles and contents of the grapes of each variety were found. The tri-hydroxylated molecules were prevalent respect to the di-hydroxylated anthocyanins in the three varieties. Tannat skins had the highest proportion of non-acylated glucosides, petunidin and delphinidin derivatives. Cabernet Sauvignon skins had the highest proportions of acetylated glucosides and malvidin derivatives. Merlot skins presented the highest proportions of coumaroyl glucosides and peonidin derivatives. It can be concluded that the grape variety and the environmental conditions determined important differences in the anthocyanin composition of grapes and the extractability of these compounds from the skins. The anthocyanin contents and profiles of the grapes of each variety presented high differences that might determine important variations in the colour and composition of the respective wines.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Gustavo González Neves*, Diego Piccardo, Graciela Gil, Guzmán Favre, Laura Barreiro, Milka Ferrer

*Universidad de la república

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Fractionation of copper and iron in wine: Assessment of potential macromolecule and sulfur binding agents

Copper and iron are known to substantially impact wine stability through oxidative, reductive or colloidal phenomena. However, the binding of metal ions to different wine components under wine conditions, and the impact of this binding on the ability of the metal ions to induce spoilage processes, is not well understood. This study surveyed a range of red and white wines for an understanding of the variability of broad metal categories within the wines. The techniques utilized included an electrochemical constant current stripping potentiometry technique (ccSP), and solid phase extraction (SPE) fractionation of wine with subsequent analysis of the metal content of each fraction by inductively coupled plasma – optical emission spectroscopy (ICP-OES).

DNA and type of grain: which factor does better explain sensory differences of sessile and pedunculate oaks?

Sessile oak and pedunculate oak have shown several differences of interest for enological purposes. Tannic and aromatic composition among sessile oak or pedonculate oak has been well studied. Sessile oak is generally more aromatic than pedunculated, while the later is more tannic. This scientific point of view is rarely applied to classify oak in cooperages. Most coopers use the type of grain to distinguish wide and thin grain.

Sensory impacts of the obturator used for the Chasselas: study over the time

Many parameters affect the organoleptic characteristics of wine: internal parameters like the chemical composition or polyphenol content and external as for example storage conditions or the type of obturator. The aim of this study was to characterize sensorally the impacts of several type of obturator on a white wine: Chasselas. To determine the organoleptic characteristics of this wine, a quantitative descriptive analysis could be used. But rapid sensory methods were preferred in this project. Indeed these methods are an appropriate alternative to conventional descriptive methods for quickly assessing sensory product discrimination.

Interaction between the enzymes of central carbon metabolism and anthocyanin biosynthesis during grape berry development

Primary and secondary metabolites are major components of grape quality and wine typicity. Their accumulation is interconnected through a complex metabolic network, which is still not well understood. This study aims to investigate how the enzymes of central carbon metabolism interact with anthocyanin biosynthesis during grape berry development: does the accumulation of anthocyanins, which represents a non-negligible diversion of carbon metabolic fluxes, require reprogramming of central enzymes or is it controlled downstream of central metabolism? To this end, 23 enzymes involved in central carbon metabolism pathways have been analyzed in the berries of 3 grape cultivars, which have close genetic background but distinct temporal dynamics of anthocyanin accumulation.

Simultaneous monitoring of dissolved CO2 and collar from Rosé sparkling wine glasses: the impact of yeast macromolecules

Champagne or sparkling wines elaborated through the same traditional method, which consists in two major yeast-fermented steps, typically hold about 10 to 12 g/L of dissolved CO2 after the second fermentation in a closed bottle. Hundreds of molecules and macromolecules originating from grape and yeast cohabit with dissolved CO2; they are essential compounds contributing to many organoleptic characteristics (effervescence, foam, aroma, taste, colour…). Indeed, the second alcoholic fermentation and the maturation on lees (which may last from 12 months up to several years) both induce various quantitative and qualitative changes in the wine through the action of yeast, as listed hereafter: development of aromas during aging on lees, release of nitrogen compounds during autolysis and release of macromolecules (polysaccharides, lipids, nucleic acids) in wine.