Macrowine 2021
IVES 9 IVES Conference Series 9 Metabolomic profile of red non-V. vinifera genotypes

Metabolomic profile of red non-V. vinifera genotypes

Abstract

Vitis vinifera L. is the most widely cultivated Vitis species which includes numerous cultivars. Owing to their superior quality of grapes, these cultivars were long considered the only suitable for the production of fine wines. However, the lack of resistance genes in V. vinifera against major grapevine pathogens, requires for its cultivation frequent spraying with large amount of fungicides. Thus, the search for alternative and more sustainable methods to control the grapevine pathogens have brought the breeders to focus their attention on other Vitis species. In fact, wild Vitis genotypes present multiple resistance traits against pathogens, such as powdery mildew, downy mildew and phylloxera. For this reason, these genotypes have been used in breeding programs in order to introgress resistant traits to pests and diseases in V. vinifera species. The aim of this work was to study the metabolite profiles (simple phenolic compounds, anthocyanins, lipids and proanthocyanidins) of two hybrid varieties (41B and K5BB) and seven American Vitis species (V. andersonii, V. arizonica, V. champinii, V. cinerea, V. riparia, V. slavinii and V. californica) in six different vintages. The results were compared with two V. vinifera cultivars (Pinot Noir and Cabernet Sauvignon). Grape skin anthocyanins were analyzed by HPLC-DAD [1] and twenty different anthocyanins were detected and quantified. In four genotypes less than 5% of the total amount of anthocyanins detected were diglucosides (from 11.6 to 56.9 mg/kg). In the five remaining genotypes more than 50% of the total were found to be diglucosides (from 522.1 to 2657.6 mg/kg). Analysis of phenolic compounds by UHPLC-MS/MS [2] showed that three non-V. vinifera genotypes contained higher average amount of total phenolics compared to V. vinifera cultivars. A rapid LC-MS/MS method [3] was used to identify and quantify thirty-three lipids. The total lipids of eight out of nine non-V. vinifera genotypes was higher compared to V. vinifera cultivars. The differences between the genotypes’ content of anthocyanins, phenolics and lipids were investigated by heatmap analysis. The data was scaled to unit variance, the correlation of variables was used as the distance measure and Ward’s minimum variance method was used for hierarchical clustering. Up to our knowledge this is the most extended metabolomics profiling study on wild Vitis grape genotypes. Altogether, this study highlights the presence of a significant genotypic diversity between the composition of the fruits of V. vinifera and other species. The knowledge of their composition can greatly influence the further breeding programs, since being responsible for both the quality and the resistance traits of new grape interspecific varieties.

[1] Mattivi F. et al. JAFC 2006, 54, 7692-7702 [2] Vrhovsek U. et al. JAFC 2012, 60, 8831-8840 [3] Della Corte A. et al. Talanta 2015, 140, 52-61

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Article

Authors

Silvia Ruocco*, Daniele Perenzoni, Fulvio Mattivi, Jan Stanstrup, Marco Stefanini, Urska Vrhovsek

*Fondazione Edmund Mach

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Partial dealcoholisation of red wine by reverse osmosis-evaporative perstraction: impact on wine composition

Around the world, the alcohol content of wine has been steadily increasing; partly as a consequence of climate change, but also due to improvements in viticultural management practices and winemaking techniques [1,2]. Concurrently, market demand for wines with lower alcohol levels has increased as consumers seek to reduce alcohol intake for social and/or health reasons [3]. As such, there is increasing demand for both innovative methods that allow winemakers to produce ‘reduced alcohol wines’ (RAW) and a better understanding of the impact of such methods on the composition of RAW. This study therefore aimed to investigate compositional changes in two red wines resulting from partial alcohol removal following treatment by one such method, involving a combination of reverse osmosis and evaporative perstraction (RO-EP).

Modulating role of SO2 in white wine protein haze formation

Despite the extensive research performed during the last decades, the multifactorial mechanism responsible for the white wine protein haze formation is not fully characterized. Herein, a new model is proposed, which is based on the experimental identification of sulfur dioxide as a major modulating factor inducing wine protein haze upon heating. As opposed to other reducing agents, such as 2-mercaptoethanol, dithiothreitol and tris(2-carboxyethyl)phosphine hydrochloride (TCEP), the addition of SO2 to must/wine upon heating cleaves intraprotein disulfide bonds, hinders thiol-disulfide exchange during protein interactions and can lead to the formation of novel inter/intraprotein disulfide bonds. Those are eventually responsible for wine protein aggregation which follows a nucleation-growth kinetic model as shown by dynamic light scattering [1].

Effect of the winemaking technology on the phenolic compounds, foam parameters in sparklig wines

Contribution Sparkling wines elaborated following the traditional method undergo a second fermentation in closed bottles of base wines, followed by aging of wines with lees for at least 9 months. Most of the sparkling wines elaborated are white and rosé ones, although the production of red ones is highly increasing. One of the initial problems in red sparkling wine processing is to obtain suitable base wines that should have moderate alcohol content and astringency and adequate color intensity; which is difficult to obtain when grapes must be harvested at low phenolic and industrial maturity stage. The low phenolic maturity degree in the red grapes makes essential to choose an adequate winemaking methodology to obtain the base wines because the extracted polyphenols will vary according the winemaking technique: carbonic maceration or destemmed-crushed grapes.

Oak wood seasoning: impact on oak wood chemical composition and sensory quality of wine

Oak wood selection and maturation are essential steps in the course of barrel fabrication. Given the existence of many factors involved in the choice of raw material and in natural seasoning of oak wood, it is very difficult to determine the real impact of seasoning and selection factors on oak wood composition. A sampling was done to study the evolution of oak wood chemical composition during four seasoning steps: non matured, 12 months, 18 months and 24 months. For this sampling, three selection factors were taken into account: age, grain type and the Polyphenolic Index measured by Oakscan®. Besides extractables
(~10%), three polymers constitute the main part of oak wood: cellulose, hemicelluloses and lignins.

Characterization of free and glycosidically bound simple phenols in hybrid grape varieties using liquid chromatography coupled to high resolution mass (q-orbitrap)

Vitis vinifera is one of the most diffused grapevines over the word and it is the raw material for high quality wines production. The availability of more resistant interspecific hybrid vine varieties, developed from crosses between Vitis vinifera and other Vitis species, has generating much interest, also due to the low environmental effect of production. However, hybrid grape wine composition and varietal differences between interspecific hybrids are not well defined. Different studies revealed that wine consumption has health effects due to its high content of antioxidants, as phenolic compounds. In particular, simple phenols are appreciated not only for their physiological health benefits, including antioxidant, anti-inflammatory and cardioprotective effects, but also because they affect wines organoleptic profile and have a significant role in defining their nutritional characteristics.