Macrowine 2021
IVES 9 IVES Conference Series 9 Metabolomic profile of red non-V. vinifera genotypes

Metabolomic profile of red non-V. vinifera genotypes

Abstract

Vitis vinifera L. is the most widely cultivated Vitis species which includes numerous cultivars. Owing to their superior quality of grapes, these cultivars were long considered the only suitable for the production of fine wines. However, the lack of resistance genes in V. vinifera against major grapevine pathogens, requires for its cultivation frequent spraying with large amount of fungicides. Thus, the search for alternative and more sustainable methods to control the grapevine pathogens have brought the breeders to focus their attention on other Vitis species. In fact, wild Vitis genotypes present multiple resistance traits against pathogens, such as powdery mildew, downy mildew and phylloxera. For this reason, these genotypes have been used in breeding programs in order to introgress resistant traits to pests and diseases in V. vinifera species. The aim of this work was to study the metabolite profiles (simple phenolic compounds, anthocyanins, lipids and proanthocyanidins) of two hybrid varieties (41B and K5BB) and seven American Vitis species (V. andersonii, V. arizonica, V. champinii, V. cinerea, V. riparia, V. slavinii and V. californica) in six different vintages. The results were compared with two V. vinifera cultivars (Pinot Noir and Cabernet Sauvignon). Grape skin anthocyanins were analyzed by HPLC-DAD [1] and twenty different anthocyanins were detected and quantified. In four genotypes less than 5% of the total amount of anthocyanins detected were diglucosides (from 11.6 to 56.9 mg/kg). In the five remaining genotypes more than 50% of the total were found to be diglucosides (from 522.1 to 2657.6 mg/kg). Analysis of phenolic compounds by UHPLC-MS/MS [2] showed that three non-V. vinifera genotypes contained higher average amount of total phenolics compared to V. vinifera cultivars. A rapid LC-MS/MS method [3] was used to identify and quantify thirty-three lipids. The total lipids of eight out of nine non-V. vinifera genotypes was higher compared to V. vinifera cultivars. The differences between the genotypes’ content of anthocyanins, phenolics and lipids were investigated by heatmap analysis. The data was scaled to unit variance, the correlation of variables was used as the distance measure and Ward’s minimum variance method was used for hierarchical clustering. Up to our knowledge this is the most extended metabolomics profiling study on wild Vitis grape genotypes. Altogether, this study highlights the presence of a significant genotypic diversity between the composition of the fruits of V. vinifera and other species. The knowledge of their composition can greatly influence the further breeding programs, since being responsible for both the quality and the resistance traits of new grape interspecific varieties.

[1] Mattivi F. et al. JAFC 2006, 54, 7692-7702 [2] Vrhovsek U. et al. JAFC 2012, 60, 8831-8840 [3] Della Corte A. et al. Talanta 2015, 140, 52-61

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Article

Authors

Silvia Ruocco*, Daniele Perenzoni, Fulvio Mattivi, Jan Stanstrup, Marco Stefanini, Urska Vrhovsek

*Fondazione Edmund Mach

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Impact of drought stress on concentration and composition of wine proteins in Riesling

Protein haze in white wines is a major technological and economic problem of the wine industry. Field tests were carried out in steep slope vineyards planted with Riesling grapes over 3 dry growing seasons to study the effect of drought stress on the concentration of proteins in the resulting wines. Plots suffering from drought stress were compared with surrounding drip irrigated plots. Riesling grapes were processed into wines by conventional procedures. Protein amounts of the isolated wine colloids of the stressed samples were always higher than those of the watered samples(mean watered 13.8 ± 0.44, mean stressed 17.4 ± 0.40 g 100 g-1). As a consequence, higher bentonite doses were needed to achieve protein haze stability of the drought stressed treatments.

Fractionation of copper and iron in wine: Assessment of potential macromolecule and sulfur binding agents

Copper and iron are known to substantially impact wine stability through oxidative, reductive or colloidal phenomena. However, the binding of metal ions to different wine components under wine conditions, and the impact of this binding on the ability of the metal ions to induce spoilage processes, is not well understood. This study surveyed a range of red and white wines for an understanding of the variability of broad metal categories within the wines. The techniques utilized included an electrochemical constant current stripping potentiometry technique (ccSP), and solid phase extraction (SPE) fractionation of wine with subsequent analysis of the metal content of each fraction by inductively coupled plasma – optical emission spectroscopy (ICP-OES).

The effect of Nitrogen and Sulphur foliar applications in hot climates

ine nitrogen deficiency can negatively influence the aroma profile and ageing potential of white wines. Canopy management can alter vine microclimate, affect the nitrogen availability and influence the response of leaf senescence. Increasing the nitrogen availability to vines can increase the Yeast Assimilable Nitrogen (YAN) levels in harvested fruit and wine. Studies show that foliar nitrogen and sulphur applications at véraison, on low YAN Sauvignon blanc grapes have an effect on the level of amino acids (Jreij et al. 2009) and on S-containing compounds such as glutathione and thiols (Lacroux et al. 2008), which in turn can influence the formation of major volatiles and the aroma profile of the wine.

Nitrogen – Lipid Balance in alcoholic fermentations. Example of Champagne musts

Nutrient availability – nitrogen, lipids, vitamins or oxygen – has a major impact on the kinetics of winemaking fermentations. Nitrogen is usually the growth-limiting nutrient and its availability determines the fermentation rate, and therefore the fermentation duration. In some cases, in particular in Champagne, grape musts have high nitrogen concentrations and are sometimes clarified with turbidity below 50 NTU. In these conditions, lipid deficiencies may occur and longer fermentations can be observed. To better understand this situation, a study was realized using a synthetic medium simulating the composition of a Champagne must : 180 g/L of sugar, 360 mg/L of assimilable nitrogen and a lipid content ranging from 1 to 8 mg/L of phytosterols (mainly β-sitosterol).

Analysis of voltammetric fingerprints of different white grape musts reveals genotype-related oxidation patterns

Must oxidation is a complex process involving multiple enzymatic transformations, including the oxidation of phenolics containing an ortho-diphenol function. The latter process has a primary influence on wine aroma characteristics and stability, due to the central role of ortho-diphenols in the non-enzymatic oxidative reactions taking place during winemaking and in finished wine. Although oxidation of must is traditionally avoided, in recent years its contribution to wine quality has been revisited, and in some cases improvements to wine aroma have been observed with the application of controlled must oxidation. Nowadays there is a great interest in the wine industry towards the identification of specific markers or patterns to characterize and classify the response of grape must to oxidation.